Correlations of the frequency dependence of the ultrasonic backscatter coefficient with the bone volume fraction and the trabecular thickness in bovine trabecular bone: Application of the binary mixture model.

The ultrasonic backscatter coefficient and the exponent n (frequency dependence of the backscatter coefficient) were measured in 24 bovine femoral trabecular bone samples. The binary mixture model for ultrasonic scattering from trabecular bone was applied to predict the variations of the ultrasound parameters with the bone volume fraction (BV/TV) and the trabecular thickness (Tb.Th) in trabecular bone. The backscatter coefficient exhibited significant, positive correlations with the BV/TV (R = 0.82) and the Tb.Th (R = 0.79). In contrast, the exponent n was found to be significantly, negatively correlated with the BV/TV (R = -0.77) and the Tb.Th, (R = -0.71).

Thickness dependences of acoustic bandgaps with different generation mechanisms in phononic crystals immersed in water.

The thickness dependences of acoustic bandgaps were theoretically and experimentally investigated in two-dimensional phononic crystals (PCs) immersed in water. The acoustic pressure transmission coefficients were measured as a function of the PC thickness in order to understand the characteristics of the transmission loss through the PCs. The acoustic bandgaps can be classified into two types of generation mechanisms from the perspective of acoustic diffraction modes: Bragg bandgap and non-zeroth order diffraction (NZOD) bandgap. The NZOD bandgaps show larger transmission losses and shorter decay lengths with increasing PC thickness than the Bragg bandgaps.

Velocity dispersion and backscatter in marrow-filled and water-filled trabecular bone samples in vitro.

The phase velocity and the backscatter coefficient were measured in 28 bovine femoral trabecular bone samples filled with marrow and water in vitro from 0.2 to 0.6 MHz. The phase velocities decreased approximately linearly with increasing frequency and the average dispersion rate of -34 ms-1 MHz-1 in the marrow-filled samples was higher than that of -42 ms-1 MHz-1 in the water-filled samples. The backscatter coefficients exhibited nonlinear, monotonically increasing dependences on the frequency and the average value of the exponent n = 2.92 (frequency dependence) in the marrow-filled samples was higher than the value of n = 2.79 in the water-filled samples.

Zeroth-order resonance phenomenon in an acoustic composite right/left-handed metamaterial resonator.

This study proposes an acoustic theory that describes the resonance phenomena in a resonator made of acoustic composite right/left-handed (CRLH) metamaterials, and verifies it through numerical simulation. The established theory for a microwave CRLH metamaterial resonator is adapted to explain the resonance phenomena in an acoustic CRLH metamaterial resonator. In particular, attention is focused on the zeroth-order resonance phenomenon which has several interesting properties. When a resonator is composed of a CRLH metamaterial, a resonance with a flat acoustic field distribution may occur at one of the frequencies where the wavenumber becomes zero. This resonance is called zeroth-order resonance. Through numerical simulation, such unusual resonance phenomenon in acoustics is observed in more detail and the proposed theory is verified. The results of the theory and the numerical simulation clearly show that zeroth-order resonance can exist at those frequencies where the acoustic field distribution is flat due to infinite wavelength. It is also shown that the resonance frequency and the Q factor of this resonance depend on the boundary condition at both ends of the resonator, and they basically do not change even when the number of units is reduced or increased.

Relationships of linear and nonlinear ultrasound parameters with porosity and trabecular spacing in trabecular-bone-mimicking phantoms.

The speed of sound (SOS), the normalized broadband ultrasound attenuation (nBUA), and the nonlinear parameter (B/A) were measured in 18 trabecular-bone-mimicking phantoms consisting of water-saturated aluminum foams. The strong slow wave and the very weak fast wave were consistently observed in the signals transmitted through all of the phantoms. It was found that the SOS increased as the porosity and the trabecular spacing increased. In contrast, both the nBUA and the B/A showed opposite dependences on the porosity and the trabecular spacing. All three ultrasound parameters exhibited high correlation coefficients with the porosity and the trabecular spacing.

The anti-hypertensive drug reserpine induces neuronal cell death through inhibition of autophagic flux.

Reserpine is a well-known medicine for the treatment of hypertension and schizophrenia, but its administration can induce Parkinson's disease (PD)-like symptoms in humans and animals. Reserpine inhibits the vesicular transporter of monoamines and depletes the brain of monoamines such as dopamine. However, the cellular function of reserpine is not fully understood. In this report, we present one possible mechanism by which reserpine may contribute to PD-like symptoms. Reserpine treatment induced the formation of enlarged autophagosomes by inhibiting the autophagic flux and led to accumulation of p62, an autophagy adapter molecule. In particular, reserpine treatment increased the level of α-synuclein protein and led to accumulation of α-synuclein in autophagosomes. Treatment with rapamycin enhanced the effect of reserpine by further increasing the level of α-synuclein and neuronal cell death. Drosophila raised on media containing reserpine showed loss of dopaminergic neurons. Furthermore, cotreatment with reserpine and rapamycin aggravated the loss of dopaminergic neurons. Our results suggest that reserpine contributes to the loss of dopaminergic neurons by interfering with autophagic flux.

Dependences of ultrasonic properties on frequency and trabecular spacing in trabecular-bone-mimicking phantoms.

The dependences of ultrasonic properties on the frequency and the trabecular spacing were investigated in 20 trabecular-bone-mimicking phantoms consisting of cellular copper foams. The strong slow waves were consistently observed in the signals transmitted through all of the phantoms. The frequency-dependent phase velocity and attenuation coefficient of the slow wave were measured at frequencies from 0.7 to 1.3 MHz. The phase velocity decreased approximately linearly with increasing frequency while the attenuation coefficients increased with increasing frequency. The phase velocity increased monotonically with increasing trabecular spacing from 1337 to 2931 µm while the attenuation coefficient decreased with increasing spacing.

Propagation of time-reversed Lamb waves in bovine cortical bone in vitro.

The present study aims to investigate the propagation of time-reversed Lamb waves in bovine cortical bone in vitro. The time-reversed Lamb waves were successfully launched at 200 kHz in 18 bovine tibiae through a time reversal process of Lamb waves. The group velocities of the time-reversed Lamb waves in the bovine tibiae were measured using the axial transmission technique. They showed a significant correlation with the cortical thickness and tended to follow the theoretical group velocity of the lowest order antisymmetrical Lamb wave fairly well, consistent with the behavior of the slow guided wave in long cortical bones.

Dependences of quantitative ultrasound parameters on frequency and porosity in water-saturated nickel foams.

The frequency-dependent phase velocity, attenuation coefficient, and backscatter coefficient were measured from 0.8 to 1.2 MHz in 24 water-saturated nickel foams as trabecular-bone-mimicking phantoms. The power law fits to the measurements showed that the phase velocity, the attenuation coefficient, and the backscatter coefficient were proportional to the frequency with exponents n of 0.95, 1.29, and 3.18, respectively. A significant linear correlation was found between the phase velocity at 1.0 MHz and the porosity. In contrast, the best regressions for the normalized broadband ultrasound attenuation and the backscatter coefficient at 1.0 MHz were obtained with the polynomial fits of second order.

Correlations of linear and nonlinear ultrasound parameters with density and microarchitectural parameters in trabecular bone.

In the present study, correlations of linear and nonlinear ultrasound parameters (speed of sound, normalized broadband ultrasound attenuation, and nonlinear parameter B/A) with bone mineral density and microarchitectural parameters were investigated in 28 bovine femoral trabecular bone samples in vitro. All three ultrasound parameters exhibited relatively high correlation coefficients with the indexes of bone quantity (bone mineral density and bone volume fraction) and lower correlation coefficients with the remaining microarchitectural parameters. These results suggest that B/A, in addition to speed of sound and attenuation, may have potential as an index for the assessment of bone status and osteoporosis.

Ultrasonic properties in marrow-filled and water-filled bovine femoral trabecular bones in vitro.

Phase velocity and normalized broadband ultrasound attenuation (nBUA) were measured in 22 marrow-filled and water-filled bovine femoral trabecular bone samples. Replacement of marrow by water led to a significant increase in the mean phase velocity of 47 ± 12 m/s (+3.1%), but a decrease in the mean nBUA of 10.4 ± 2.9 dB/cm/MHz (-38.9%). All the ultrasonic properties in the marrow-filled and water-filled samples exhibited significant negative Pearson's correlation coefficients of r = -0.87 to -0.92 with porosity. High correlations were also observed between pairs of the ultrasonic properties, with r = 0.85 to 0.93.

Influence of cortical endplate on speed of sound in bovine femoral trabecular bone in vitro.

Speed of sound (SOS) was measured in 14 bovine femoral trabecular bone samples with and without the cortical endplates with various thicknesses of 1.00, 1.31, 1.47, 1.75, and 2.00 mm. The presence of the cortical endplates resulted in an increase in the mean SOS of 16 m/s (+0.9%) to 91 m/s (+5.3%). The mean SOS measured in the samples with and without the cortical endplates exhibited similar significant correlations with apparent bone density (r = 0.86-0.91). All the SOS measurements were also found to be highly correlated with each other (r = 0.89-0.99).

Correlations between ultrasonic guided wave velocities and bone properties in bovine tibia in vitro.

Correlations between ultrasonic guided wave velocities and bone properties were investigated in bovine tibia in vitro. The velocities of the first arriving signal and the slow guided wave, termed V(FAS) and V(SGW), along the long axis of the tibia were measured at 200 kHz in 20 bovine tibiae using the axial transmission technique. V(FAS) yielded significant negative correlation coefficients of -0.54 to -0.66 with the bone properties. In contrast, V(SGW) yielded strong positive correlation coefficients of 0.68-0.84. The best univariate predictor of V(FAS) and V(SGW) was the cortical thickness yielding adjusted squared correlation coefficients of 0.41 and 0.69, respectively.

Scanning Deposition Method for Large-Area Diamond Film Synthesis Using Multiple Microwave Plasma Sources.

The demand for synthetic diamonds and research on their use in next-generation semiconductor devices have recently increased. Microwave plasma chemical vapor deposition (MPCVD) is considered one of the most promising techniques for the mass production of large-sized and high-quality single-, micro- and nanocrystalline diamond films. Although the low-pressure resonant cavity MPCVD method can synthesize high-quality diamonds, improvements are needed in terms of the resulting area. In this study, a large-area diamond synthesis method was developed by arranging several point plasma sources capable of processing a small area and scanning a wafer. A unit combination of three plasma sources afforded a diamond film thickness uniformity of ±6.25% at a wafer width of 70 mm with a power of 700 W for each plasma source. Even distribution of the diamond grains in a size range of 0.1-1 µm on the thin-film surface was verified using field-emission scanning electron microscopy. Therefore, the proposed novel diamond synthesis method can be theoretically expanded to achieve large-area films.

Correlations of group velocity, phase velocity, and dispersion with bone density in bovine trabecular bone.

The present study investigated the correlations of the group velocity, the phase velocity, and the velocity dispersion with the apparent bone density in bovine trabecular bone in vitro. The phase velocity exhibited the negative dispersion, consistent with the behavior in human trabecular bone. The group and the phase velocities were found to increase with increasing apparent bone density, respectively, exhibiting similar high correlations of r=0.94 and 0.96. The negative dispersion rate exhibited a decreasing dependence on the apparent bone density, with a significant correlation of r=-0.86.

Anti-contamination SMART (Spectrum Monitoring Apparatus with Roll-to-roll Transparent film) window for optical diagnostics of plasma systems.

Optical emission spectroscopy is widely used in semiconductor and display manufacturing for plasma process monitoring. However, because of the contamination of the viewport, quantitative analysis is extremely difficult; therefore, qualitative analysis is used to detect species in the process. To extend plasma monitoring in advanced precise processes, the contamination problem of the viewport must be solved. We propose a new spectrum monitoring apparatus with a roll-to-roll transparent film window for optical diagnostics of a plasma system. By moving a transparent film in front of the viewport, contamination in the emission light path becomes negligible. However, the speed of the film should be optimized to reduce the maintenance period and to minimize measurement errors. We calculated the maximum thickness of SiO2, Si3N4, ITO, and the Ar/CHF3 plasma contaminant to suppress the electron temperature error measured by the line-intensity-ratio within 2% at 2 eV. The thickness of the Si3N4, ITO, and Ar/CHF3 plasma contaminant should be thinner than 12.5 nm, 7.5 nm, and 100 nm, respectively.

Diffusion-Weighted Imaging Findings of Ischemic Spinal Injury in a Chondrodystrophic Dog With Fibrocartilaginous Embolism.

A 9-year-old, intact male Shih Tzu dog presented with systemic weakness and peracute onset of tetraplegia. Tetraplegia with lower motor neuron signs was noted upon neurological examination. Diseases that cause acute flaccid tetraparesis, such as acute fulminating myasthenia gravis, polyradiculoneuritis, tick paralysis, and botulism, were ruled out based on the medical history, normal electrophysiological tests, and non-response to the neostigmine challenging test. Initial 0.3-Tesla (T) magnetic resonance imaging (MRI) findings included sharply demarcated intramedullary lesions at the C3-C6 level, mainly involving gray matter, which appeared hypo- to iso- intense on T1-weighted images (WIs), and hyperintense on T2-WIs and fluid-attenuated inversion recovery images. There was no enhancement on post-contrast T1-WIs. Neutrophilic pleocytosis was observed in the cerebrospinal fluid analysis. No clinical responses were observed for the treatment of non-infectious myelitis with an immunosuppressive dosage of prednisolone. A follow-up 3-T MRI 6 days later demonstrated hyperintensity on diffusion-WI (DWI) and a decreased apparent diffusion coefficient (ADC) value (0.54 × 10-3 mm2/s) of the spinal lesions. Through histological examination, a fibrocartilaginous embolism was definitively confirmed. This is the first report to describe an ischemic spinal injury visualized by DWI and ADC mapping with high-field MRI in a chondrodystrophic dog diagnosed with a fibrocartilaginous embolism.

Empirical angle-dependent Biot and MBA models for acoustic anisotropy in cancellous bone.

The Biot and the modified Biot-Attenborough (MBA) models have been found useful to understand ultrasonic wave propagation in cancellous bone. However, neither of the models, as previously applied to cancellous bone, allows for the angular dependence of acoustic properties with direction. The present study aims to account for the acoustic anisotropy in cancellous bone, by introducing empirical angle-dependent input parameters, as defined for a highly oriented structure, into the Biot and the MBA models. The anisotropy of the angle-dependent Biot model is attributed to the variation in the elastic moduli of the skeletal frame with respect to the trabecular alignment. The angle-dependent MBA model employs a simple empirical way of using the parametric fit for the fast and the slow wave speeds. The angle-dependent models were used to predict both the fast and slow wave velocities as a function of propagation angle with respect to the trabecular alignment of cancellous bone. The predictions were compared with those of the Schoenberg model for anisotropy in cancellous bone and in vitro experimental measurements from the literature. The angle-dependent models successfully predicted the angular dependence of phase velocity of the fast wave with direction. The root-mean-square errors of the measured versus predicted fast wave velocities were 79.2 m s(-1) (angle-dependent Biot model) and 36.1 m s(-1) (angle-dependent MBA model). They also predicted the fact that the slow wave is nearly independent of propagation angle for angles about 50 degrees , but consistently underestimated the slow wave velocity with the root-mean-square errors of 187.2 m s(-1) (angle-dependent Biot model) and 240.8 m s(-1) (angle-dependent MBA model). The study indicates that the angle-dependent models reasonably replicate the acoustic anisotropy in cancellous bone.

Phase velocity and normalized broadband ultrasonic attenuation in Polyacetal cuboid bone-mimicking phantoms.

The variations of phase velocity and normalized broadband ultrasonic attenuation (nBUA) with porosity were investigated in Polyacetal cuboid bone-mimicking phantoms with circular cylindrical pores running normal to the surface along the three orthogonal axes. The frequency-dependent phase velocity and attenuation coefficient in the phantoms with porosities from 0% to 65.9% were measured from 0.65 to 1.10 MHz. The results showed that the phase velocity at 880 kHz decreased linearly with porosity, whereas the nBUA increased linearly with porosity. This study provides a useful insight into the relationships between ultrasonic properties and porosity in bone at porosities lower than 70%.

Relationships of the group velocity of the time-reversed Lamb wave with bone properties in cortical bone in vitro.

The present study aims to investigate the feasibility of using the time-reversed Lamb wave as a new method for noninvasive characterization of long cortical bones. The group velocity of the time-reversed Lamb wave launched by using the modified time reversal method was measured in 15 bovine tibiae, and their correlations with the bone properties of the tibia were examined. The group velocity of the time-reversed Lamb wave showed significant positive correlations with the bone properties (r=0.55-0.81). The best univariate predictor of the group velocity of the time-reversed Lamb wave was the cortical thickness, yielding an adjusted squared correlation coefficient (r2) of 0.64. These results imply that the group velocity of the time-reversed Lamb wave, in addition to the velocities of the first arriving signal and the slow guided wave, could potentially be used as a discriminator for osteoporosis.