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3.
Histochemistry ; 64(3): 319-22, 1979.
Artigo em Inglês | MEDLINE | ID: mdl-521318

RESUMO

Cathepsin B was demonstrated cytochemically in human fibroblasts with Z-Ala-Arg-Arg-2-(4-methoxy)naphtylamide as substrate. The enzyme was visualized in the bright field microscope with the diazonium salt Fast Blue B as coupling reagent and in the fluorescence microscope with 5-nitrosalicylaldehyde. With both methods cathepsin B was found in small granules distributed throughout the cytoplasm.


Assuntos
Carcinoma de Células Escamosas/enzimologia , Catepsinas/análise , Carcinoma de Células Escamosas/patologia , Fibroblastos/enzimologia , Histocitoquímica , Humanos , Microscopia de Fluorescência
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