RESUMO
The cnidarian freshwater polyp Hydra sp. exhibits an unparalleled regeneration capacity in the animal kingdom. Using an integrative transcriptomic and stable isotope labeling by amino acids in cell culture proteomic/phosphoproteomic approach, we studied stem cell-based regeneration in Hydra polyps. As major contributors to head regeneration, we identified diverse signaling pathways adopted for the regeneration response as well as enriched novel genes. Our global analysis reveals two distinct molecular cascades: an early injury response and a subsequent, signaling driven patterning of the regenerating tissue. A key factor of the initial injury response is a general stabilization of proteins and a net upregulation of transcripts, which is followed by a subsequent activation cascade of signaling molecules including Wnts and transforming growth factor (TGF) beta-related factors. We observed moderate overlap between the factors contributing to proteomic and transcriptomic responses suggesting a decoupled regulation between the transcriptional and translational levels. Our data also indicate that interstitial stem cells and their derivatives (e.g., neurons) have no major role in Hydra head regeneration. Remarkably, we found an enrichment of evolutionarily more recent genes in the early regeneration response, whereas conserved genes are more enriched in the late phase. In addition, genes specific to the early injury response were enriched in transposon insertions. Genetic dynamicity and taxon-specific factors might therefore play a hitherto underestimated role in Hydra regeneration.
Assuntos
Regulação da Expressão Gênica/fisiologia , Hydra/fisiologia , Regeneração/fisiologia , Transcriptoma/fisiologia , Via de Sinalização Wnt/fisiologia , Animais , Perfilação da Expressão Gênica/métodosRESUMO
Biallelic mutations in the gene encoding DHOdehase [dihydroorotate dehydrogenase (DHODH)], an enzyme required for de novo pyrimidine biosynthesis, have been identified as the cause of Miller (Genée-Weidemann or postaxial acrofacial dysostosis) syndrome (MIM 263750). We report compound heterozygous DHODH mutations in four additional families with typical Miller syndrome. Complementation in auxotrophic yeast demonstrated reduced pyrimidine synthesis and in vitro enzymatic analysis confirmed reduced DHOdehase activity in 11 disease-associated missense mutations, with 7 alleles showing discrepant activity between the assays. These discrepancies are partly explained by the domain structure of DHODH and suggest both assays are useful for interpretation of individual alleles. However, in all affected individuals, the genotype predicts that there should be significant residual DHOdehase activity. Urine samples obtained from two mutation-positive cases showed elevated levels of orotic acid (OA) but not dihydroorotate (DHO), an unexpected finding since these represent the product and the substrate of DHODH enzymatic activity, respectively. Screening of four unrelated cases with overlapping but atypical clinical features showed no mutations in either DHODH or the other de novo pyrimidine biosynthesis genes (CAD, UMPS), with these cases also showing normal levels of urinary OA and DHO. In situ analysis of mouse embryos showed Dhodh, Cad and Umps to be strongly expressed in the pharyngeal arch and limb bud, supporting a site- and stage-specific requirement for de novo pyrimidine synthesis. The developmental sensitivity to reduced pyrimidine synthesis capacity may reflect the requirement for an exceptional mitogenic response to growth factor signalling in the affected tissues.
Assuntos
Anormalidades Múltiplas/enzimologia , Deformidades Congênitas dos Membros/enzimologia , Disostose Mandibulofacial/enzimologia , Micrognatismo/enzimologia , Oxirredutases atuantes sobre Doadores de Grupo CH-CH/deficiência , Anormalidades Múltiplas/genética , Anormalidades Múltiplas/urina , Animais , Sequência de Bases , Carbamoil Fosfato Sintase (Glutamina-Hidrolizante)/genética , Carbamoil Fosfato Sintase (Glutamina-Hidrolizante)/metabolismo , Pré-Escolar , Análise Mutacional de DNA , Di-Hidro-Orotato Desidrogenase , Embrião de Mamíferos/metabolismo , Embrião de Mamíferos/patologia , Feminino , Cromatografia Gasosa-Espectrometria de Massas/normas , Regulação da Expressão Gênica no Desenvolvimento , Estudos de Associação Genética , Teste de Complementação Genética , Humanos , Lactente , Botões de Extremidades/metabolismo , Botões de Extremidades/patologia , Deformidades Congênitas dos Membros/genética , Deformidades Congênitas dos Membros/urina , Masculino , Disostose Mandibulofacial/genética , Disostose Mandibulofacial/urina , Camundongos , Micrognatismo/genética , Micrognatismo/urina , Complexos Multienzimáticos/genética , Complexos Multienzimáticos/metabolismo , Mutação de Sentido Incorreto , Orotato Fosforribosiltransferase/genética , Orotato Fosforribosiltransferase/metabolismo , Ácido Orótico/análogos & derivados , Ácido Orótico/urina , Orotidina-5'-Fosfato Descarboxilase/genética , Orotidina-5'-Fosfato Descarboxilase/metabolismo , Oxirredutases atuantes sobre Doadores de Grupo CH-CH/genética , Oxirredutases atuantes sobre Doadores de Grupo CH-CH/metabolismo , Linhagem , Padrões de Referência , Schizosaccharomyces/genética , Schizosaccharomyces/crescimento & desenvolvimento , Proteínas de Schizosaccharomyces pombe/genéticaRESUMO
Wnt genes and beta-catenin signaling are involved in axial patterning processes in vertebrate embryogenesis in setting up the Spemann-Mangold organizer in amphibian embryos. An organizer with a similar function is present in the hypostome of an adult Hydra polyp. Previously, a Hydra ortholog of Wnt3 (HyWnt3), which is expressed in the hypostome, has been described. Here, ten additional Hydra Wnt genes have been identified. Of these, six (HyWnt1, -7, -9/10a, -9/10c, -11, and -16) are expressed in the adult hypostome. And, as is HyWnt3, these six Wnt genes are also expressed when a new head organizer is formed during head regeneration and bud formation. The kinetics of Wnt gene expressions during head regeneration suggests that a cascade of consecutive Wnt activation accompanies regeneration, and HyWnt3 begins this cascade. Recombinant HyWnt3 protein induced body column tissue to undergo head formation. It also increased the head formation capacity in the head regeneration-deficient mutant strain reg-16 to that of wild-type strains. In addition our data reveal striking similarities in the molecular basis of the organizer in Hydra and axis polarization in chordates (e.g. Spemann's organizer) as well as it's role in regeneration suggesting a conserved function of Wnt signaling in setting up this ancient metazoan signaling center.
Assuntos
Hydra/fisiologia , Regeneração/fisiologia , Proteínas Wnt/metabolismo , Animais , Padronização Corporal/fisiologia , Embrião não Mamífero/metabolismo , Evolução Molecular , Hydra/embriologia , Filogenia , Alinhamento de Sequência , Transdução de Sinais , Proteínas Wnt/genética , beta Catenina/genética , beta Catenina/metabolismoRESUMO
Cnidarians are simple metazoans with only two body layers and a primitive nervous system. They are famous for their nearly indefinite regeneration capacity. Recent work has identified most of the Wnt subfamilies and Wnt antagonists known from vertebrates in this basal animal model. Wnt signaling and BMP signaling have been shown to act in Hydra pattern formation and regeneration. Because recent genomic work in Hydra and Nematostella revealed many genes for vertebrate signaling pathways and transcription factors to be present in this more than 500 Myr-year-old phylum, future work will focus on the function and expression of these genes in Hydra pattern formation and regeneration. This chapter presents an in situ hybridization protocol, which is largely based on a lab protocol of the Bode lab that has proven to be extremely useful in the characterization of many developmental genes from Hydra.