RESUMO
Cancer and bacterial infections are among the leading causes of death worldwide. Plant-derived bioactive compounds constitute promising alternatives for development of new therapeutics. This study aimed at evaluating the biological activity of Withaferin A using 6 tumor cell lines: A549 (lung cancer), U87MG (glioblastoma), SH-SY5Y (neuroblastoma), B16-F10 (mouse melanoma), HeLa (uterine colon cancer) and K562 (chronic myeloid leukemia). In addition, 17 other standard bacterial strains and several multidrug resistant bacteria (MDR) clinical isolates were examined. Cell viability was assessed using the following assays: MTT, neutral red, and dsDNA PicoGreen®. Further, oxidative stress was measured by quantification of reactive oxygen species (ROS) production. The activity against bacteria was determined by the minimum inhibitory concentration (MIC), minimum bacterial concentration (CBM) and antibiofilm activity in the production of strains. Withaferin A was effective, as evidenced by its cytotoxic activity in tumor cell lines, enhanced ROS production in tumor cells and bactericidal and antibiofilm activity. Data demonstrated that Withaferin A may be therapeutically considered as an antitumor and antibacterial agent.
Assuntos
Biofilmes , Neuroblastoma , Animais , Antibacterianos/farmacologia , Bactérias/metabolismo , Humanos , Camundongos , Testes de Sensibilidade Microbiana , Espécies Reativas de Oxigênio/metabolismo , VitanolídeosAssuntos
Neoplasias Esofágicas/diagnóstico por imagem , Leiomiomatose/diagnóstico por imagem , Nefrite Hereditária/diagnóstico por imagem , Adolescente , Anastomose Cirúrgica , Transtornos de Deglutição/diagnóstico por imagem , Transtornos de Deglutição/etiologia , Transtornos de Deglutição/genética , Transtornos de Deglutição/cirurgia , Endoscopia do Sistema Digestório , Neoplasias Esofágicas/genética , Neoplasias Esofágicas/cirurgia , Esofagectomia , Seguimentos , Humanos , Leiomiomatose/genética , Leiomiomatose/cirurgia , Imageamento por Ressonância Magnética , Masculino , Invasividade Neoplásica , Nefrite Hereditária/genética , Nefrite Hereditária/cirurgia , Estômago/cirurgia , UltrassonografiaRESUMO
Principally, surgical procedures in children should be performed in specialized pediatric centers. Some minor procedures on older children could eventually take place in general hospitals, provided that pediatric medical and nursing competence is available. Adequate infrastructure and an environment appropriate for children are required as well. The comfort of therapy in an adjacent general hospital should not lead to an increased risk for the child. Principles of clinical diagnosis and treatment strategies of frequent pediatric urological entities are described and discussed in this article.