RESUMO
A method of examining cytological material during fibreoptic bronchoscopy using a methylene blue (MB) stain was assessed in 164 consecutive fibreoptic bronchoscopies where cytology specimens were taken. The MB method provided an immediate positive diagnosis in 86% of bronchoscopically visible tumours. Subsequent histology provided a positive diagnosis in 69%, conventional brush cytology in 81% and trap cytology in 77%. The MB method produced no false positive diagnosis of malignancy and the tumour cell type identified by MB stain agreed with the histological cell type in 72% of cases. This technique is considered to be sufficiently specific to provide a method of controlling the quality of specimens taken at bronchoscopy, for further analysis in the laboratory.
Assuntos
Neoplasias Brônquicas/diagnóstico , Broncoscopia/métodos , Citodiagnóstico/métodos , Azul de Metileno , Tecnologia de Fibra Óptica , Humanos , Valor Preditivo dos Testes , Estudos Retrospectivos , Coloração e RotulagemAssuntos
Carcinoma in Situ/diagnóstico , Displasia do Colo do Útero/diagnóstico , Neoplasias do Colo do Útero/diagnóstico , Esfregaço Vaginal , Carcinoma in Situ/patologia , Colposcopia , Densitometria , Feminino , Humanos , Displasia do Colo do Útero/patologia , Neoplasias do Colo do Útero/patologiaRESUMO
Herpes simplex virus type 2 is demonstrated in routine cervical smears by immunocytochemistry. Optimum antibody dilution and suitable fixation were determined and immunostaining results were found to compare favourably with those obtained by screening Papanicolaou-stained smears or by virus culture methods (virus isolation). Results obtained for immunostaining of smears from 158 patients agreed with those of virus isolation in 47 of 50 cases where virus isolation was performed. It is suggested that this immunocytochemical technique is valuable in detection of Herpes simplex virus in cervical smears, offering an inexpensive and rapid laboratory diagnosis with advantage to both patient and clinician.
Assuntos
Herpes Genital/diagnóstico , Teste de Papanicolaou , Simplexvirus/isolamento & purificação , Esfregaço Vaginal , Biópsia , Muco do Colo Uterino/microbiologia , Colo do Útero/microbiologia , Colo do Útero/patologia , Feminino , Herpes Genital/patologia , Humanos , Técnicas Imunoenzimáticas , Masculino , Simplexvirus/imunologia , Cultura de VírusRESUMO
The neuroendocrine marker neuron-specific enolase (NSE) has been reported to be detectable by immunohistochemistry in paraffin sections of neuroendocrine neoplasms of the lung. There is no study of immunodetection of NSE in cytological smear preparations from these tumours. We have examined 10 cases of small cell carcinoma of the lung, using cells obtained from serous fluids or bronchial biopsies, and found all but one had NSE-like immunoreactivity. No such immunoreactivity was found in 26 serous fluids and 19 biopsies from non-small-cell carcinomas. It is suggested, therefore, that immunostaining for NSE is a valuable aid to the cytological diagnosis of small cell carcinoma of the lung.
Assuntos
Carcinoma de Células Pequenas/diagnóstico , Neoplasias Pulmonares/diagnóstico , Neurônios/enzimologia , Fosfopiruvato Hidratase/metabolismo , Líquido Ascítico/enzimologia , Brônquios/enzimologia , Brônquios/ultraestrutura , Carcinoma de Células Pequenas/enzimologia , Carcinoma de Células Pequenas/ultraestrutura , Humanos , Técnicas Imunoenzimáticas , Neoplasias Pulmonares/enzimologia , Neoplasias Pulmonares/ultraestrutura , Microscopia Eletrônica , Derrame Pleural/enzimologiaRESUMO
Seventy consecutive lung fine needle aspirates (FNA) from 69 patients were examined by cytology/cell block histology, by transmission electron microscopy (TEM), and, where available, at histological follow up to determine the value of TEM in interpreting lung FNAs. Of 70 FNAs, 50 were suitable for TEM. Transmission electron microscopy was helpful in classifying seven of 10 poorly differentiated tumours, and two case histories are reported. In 26 of 30 well differentiated tumours TEM confirmed diagnosis by light microscopy. Two TEM false negatives, but no TEM false positives, were recorded. It is concluded that transmission electron microscopy of cells aspirated from lung can be helpful when unequivocal diagnosis has not been achieved.
Assuntos
Neoplasias Pulmonares/ultraestrutura , Pulmão/ultraestrutura , Idoso , Biópsia por Agulha , Feminino , Humanos , Neoplasias Pulmonares/diagnóstico , Masculino , Microscopia Eletrônica , Pessoa de Meia-IdadeRESUMO
The cutaneous innervation is now known to contain neuropeptides including substance P (SP) and calcitonin gene-related peptide (CGRP) in sensory nerves, and vasoactive intestinal polypeptide (VIP) and neuropeptide Y (NPY), principally in autonomic nerves. Skin biopsies from 100 leprosy patients and equivalent areas from 50 non-leprosy controls were fixed in p-benzoquinone solution for immunofluorescence staining and in Bouin's fluid for classification of leprosy type. Antisera to the neural markers, neurofilaments, and protein gene product 9.5 (PGP 9.5), and to neuropeptides were used. Cutaneous nerves and nerve endings immunoreactive for neuropeptides, neurofilaments, and PGP 9.5 were seen in all non-leprous control cases. In leprosy, PGP 9.5- and neurofilament-immunoreactive nerve fibres were seen in all 14 cases of the indeterminate (early) type and in the majority (33/43) of lepromatous cases, but in a smaller proportion (15/43) of tuberculoid cases. Neuropeptide immunoreactivity was seen in only 2/14 of the indeterminate leprosy specimens and was completely absent in other types. This early disappearance may be of diagnostic significance. Thus, cutaneous sensory and autonomic dysfunctions in leprosy are well reflected by changes in nerve fibres and neuropeptides.
Assuntos
Hanseníase/metabolismo , Neuropeptídeos/metabolismo , Pele/inervação , Calcitonina/metabolismo , Peptídeo Relacionado com Gene de Calcitonina , Humanos , Hanseníase/patologia , Neurônios/metabolismo , Neuropeptídeo Y/metabolismo , Fragmentos de Peptídeos/metabolismo , Substância P/metabolismo , Peptídeo Intestinal Vasoativo/metabolismoRESUMO
The cutaneous nerves of rat, cat, guinea pig, pig, and man were studied by immunocytochemistry to compare the staining potency of general neural markers and to investigate the density of nerves containing peptides. Antiserum to protein gene product 9.5 (PGP 9.5) stained more nerves than antisera to neurofilaments, neuron-specific enolase (NSE), and synaptophysin or histochemistry for acetylcholinesterase (AChE). Peptidergic axons showed species variation in density of distribution and were most abundant in pig and fewest in man. However, the specific peptides in nerves innervating the various structures were consistent between species. Nerve fibers immunoreactive for calcitonin gene-related peptide (CGRP) and/or vasoactive intestinal polypeptide (VIP) predominated in all the species; those immunoreactive to tachykinins (substance P and neurokinin A [NKA]) and neuropeptide tyrosine (NPY) were less abundant. Neonatal capsaicin, at the doses employed in this study, destroyed approximately 70% of CGRP- and tachykinin-immunoreactive sensory axons; whereas 6-hydroxydopamine (6-OHDA) at the doses employed resulted in a complete loss of NPY and tyrosine hydroxylase (TH) immunoreactivity without affecting VIP, CGRP, and tachykinins. Thus, this study confirms that antiserum to PGP 9.5 is the most suitable and practical marker for the demonstration of cutaneous nerves. Species differences exist in the density of peptidergic innervation, but apparently not for specific peptides. Not all sensory axons immunoreactive for CGRP and substance P/NKA are capsaicin-sensitive. However, all sympathetic TH- and NPY-immunoreactive axons are totally responsive to 6-OHDA; but no change was seen in VIP-immunoreactive axons, suggesting some demarcation of cutaneous adrenergic and cholinergic sympathetic fibers.
Assuntos
Peptídeo Relacionado com Gene de Calcitonina/análise , Neuropeptídeos/análise , Pele/inervação , Substância P/análise , Animais , Capsaicina/farmacologia , Gatos , Imunofluorescência , Cobaias , Humanos , Neurocinina A/análise , Oxidopamina/farmacologia , Ratos , Ratos Endogâmicos , Pele/química , Suínos , Ubiquitina TiolesteraseRESUMO
The brain proteins S-100 and neuron-specific enolase have been reported by separate groups to be present in human malignant melanomas. There is no systematic study comparing the occurrence of these proteins in the same tumour specimens. We have examined 33 primary malignant melanomas, including 5 which were amelanotic, and 25 metastatic melanomas using immunohistochemical methods with specific, non-cross-reacting antibodies to S-100 and NSE. We found S-100 immunoreactivity to be present in all cases but one, whereas NSE immunoreaction was very weak and patchy, and present in only 6 cases. S-100 immunoreactivity was not demonstrated in 40 control tumours, either primary or metastatic in skin, including basal- and squamous-cell carcinomas, spindle-cell sarcomas, lymphomas and Merkel cell tumours. All intradermal (n = 4) and compound (n = 1) naevi were positive for S-100, 2 blue naevi showing much less reaction. NSE immunoreactivity was detected in Merkel cell tumours (n = 8), undifferentiated (n = 2) and small cell (n = 1) carcinomas, and all melanocytic naevi. It is suggested therefore that antibody to S-100 is the reagent of choice for demonstration of melanocytic tumours, and may be especially valuable in the diagnosis of amelanotic melanoma or metastatic tumours of doubtful origin where melanoma is suspected.