1.
Chembiochem
; 23(4): e202100640, 2022 02 16.
Artigo
em Inglês
| MEDLINE
| ID: mdl-34932835
RESUMO
A genetic assay permits simultaneous quantification of two interacting proteins and their bound fraction at the single-cell level using flow cytometry. Apparent in-cellula affinities of protein-protein interactions can be extracted from the acquired data through a titration-like analysis. The applicability of this approach is demonstrated on a diverse set of interactions with proteins from different families and organisms and with in-vitro dissociation constants ranging from picomolar to micromolar.