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1.
Nature ; 627(8002): 67-72, 2024 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-38448698

RESUMO

Ordinary metals contain electron liquids within well-defined 'Fermi' surfaces at which the electrons behave as if they were non-interacting. In the absence of transitions to entirely new phases such as insulators or superconductors, interactions between electrons induce scattering that is quadratic in the deviation of the binding energy from the Fermi level. A long-standing puzzle is that certain materials do not fit this 'Fermi liquid' description. A common feature is strong interactions between electrons relative to their kinetic energies. One route to this regime is special lattices to reduce the electron kinetic energies. Twisted bilayer graphene1-4 is an example, and trihexagonal tiling lattices (triangular 'kagome'), with all corner sites removed on a 2 × 2 superlattice, can also host narrow electron bands5 for which interaction effects would be enhanced. Here we describe spectroscopy revealing non-Fermi-liquid behaviour for the ferromagnetic kagome metal Fe3Sn2 (ref. 6). We discover three C3-symmetric electron pockets at the Brillouin zone centre, two of which are expected from density functional theory. The third and most sharply defined band emerges at low temperatures and binding energies by means of fractionalization of one of the other two, most likely on the account of enhanced electron-electron interactions owing to a flat band predicted to lie just above the Fermi level. Our discovery opens the topic of how such many-body physics involving flat bands7,8 could differ depending on whether they arise from lattice geometry or from strongly localized atomic orbitals9,10.

2.
Proc Natl Acad Sci U S A ; 120(16): e2221002120, 2023 Apr 18.
Artigo em Inglês | MEDLINE | ID: mdl-37036993

RESUMO

A satisfactory material with high adsorption capacity is urgently needed to solve the serious problem of environment and human health caused by lead pollution. Herein, hydrogen-substituted graphdiyne (HsGDY) was successfully fabricated and employed to remove lead ions from sewage and lead-containing blood. The as-prepared HsGDY exhibits the highest adsorption capacity of lead among the reported materials with a maximum adsorption capacity of 2,390 mg/g, i.e., ~five times larger than that of graphdiyne (GDY). The distinguished hexagonal hole and stack mode of HsGDY allows the adsorption of more lead via its inner side adsorption mode in one single unit space. In addition, the Pb 6s and H 1s hybridization promotes the strong bonding of lead atom adsorbed at the acetylenic bond of HsGDY, contributing to the high adsorption capacity. HsGDY can be easily regenerated by acid treatment and showed excellent regeneration ability and reliability after six adsorption-regeneration cycles. Langmuir isotherm model, pseudo second order, and density functional theory (DFT) demonstrated that the lead adsorption process in HsGDY is monolayer chemisorption. Furthermore, the HsGDY-based portable filter can handle 1,000 µg/L lead-containing aqueous solution up to 1,000 mL, which is nearly 6.67 times that of commercial activated carbon particles. And, the HsGDY shows good biocompatibility and excellent removal efficiency to 100 µg/L blood lead, which is 1.7 times higher than that of GDY. These findings suggest that HsGDY could be a promising adsorbent for practical lead and other heavy metal removal.

3.
Anal Chem ; 96(25): 10365-10372, 2024 Jun 25.
Artigo em Inglês | MEDLINE | ID: mdl-38869249

RESUMO

Biomimetic cytochrome P450 for chemical activation of environmental carcinogens is an efficient in vitro model for evaluating their mutagenicity and ultimately acquiring the metabolites that cannot be easily accessed by conventional routes of organic synthesis. Different kinds of mutagen derived from polyaromatic hydrocarbons (PAHs) by metalloporphyrin/oxidant model systems have been reported, but the underlying molecular mechanisms are poorly understood. Herein, we have for the first time demonstrated an effective surface-enhanced Raman scattering (SERS) protocol to study the dynamics and biomimetic metabolic behaviors of pyrene (Pyr) in the presence of various oxygen donors. Quantitative information on the relative concentration of Pyr and its metabolites in the biomimetic system can be extracted from the SERS spectra. On the basis of our results, we conclude that the oxidative metabolism of Pyr is highly influenced by the types and concentrations of oxygen donors, leading to the formation of 1-hydroxypyrene and dioxygenated products. Besides, the addition of an appropriate amount of an organic solvent can promote the formation of secondary oxidation products. These results offer valuable insights into the dynamics of PAHs metabolism and the regulation of their metabolic pathways in biomimetic activation. In comparison to traditional liquid chromatography-mass spectrometry, the present SERS approach is more suitable for high-throughput evaluation of the metabolic process and kinetics of PAHs. We anticipate that this approach will enable a more general and comprehensive tracking of metabolic dynamics and molecular mechanisms involved in the biomimetic activation of other xenobiotics, such as procarcinogens, promutagens, and drugs.


Assuntos
Pirenos , Análise Espectral Raman , Análise Espectral Raman/métodos , Cinética , Pirenos/química , Pirenos/metabolismo , Biomimética , Hidrocarbonetos Policíclicos Aromáticos/metabolismo , Hidrocarbonetos Policíclicos Aromáticos/química , Materiais Biomiméticos/química , Materiais Biomiméticos/metabolismo , Propriedades de Superfície , Ativação Metabólica , Sistema Enzimático do Citocromo P-450/metabolismo , Oxirredução
4.
Pharmacol Res ; 203: 107162, 2024 May.
Artigo em Inglês | MEDLINE | ID: mdl-38554788

RESUMO

In American men, the incidence of prostate cancer (PC) is the highest among all types of cancer, making it the second leading cause of mortality associated with cancer. For advanced or metastatic PC, antiandrogen therapies are standard treatment options. The administration of these treatments unfortunately carries the potential risk of inducing neuroendocrine prostate cancer (NEPC). Neuroendocrine differentiation (NED) serves as a crucial indicator of prostate cancer development, encompassing various factors such as phosphatidylinositol 3-kinase/protein kinase B/mammalian target of rapamycin (PI3K/AKT/mTOR), Yes-associated protein 1 (YAP1), AMP-activated protein kinase (AMPK), miRNA. The processes of autophagy and ferroptosis (an iron-dependent form of programmed cell death) play pivotal roles in the regulation of various types of cancers. Clinical trials and preclinical investigations have been conducted on many signaling pathways during the development of NEPC, with the deepening of research, autophagy and ferroptosis appear to be the potential target for regulating NEPC. Due to the dual nature of autophagy and ferroptosis in cancer, gaining a deeper understanding of the developmental programs associated with achieving autophagy and ferroptosis may enhance risk stratification and treatment efficacy for patients with NEPC.


Assuntos
Autofagia , Ferroptose , Neoplasias da Próstata , Humanos , Ferroptose/efeitos dos fármacos , Autofagia/efeitos dos fármacos , Masculino , Neoplasias da Próstata/patologia , Neoplasias da Próstata/tratamento farmacológico , Neoplasias da Próstata/metabolismo , Animais , Transdução de Sinais/efeitos dos fármacos , Antineoplásicos/uso terapêutico , Antineoplásicos/farmacologia , Tumores Neuroendócrinos/tratamento farmacológico , Tumores Neuroendócrinos/patologia , Tumores Neuroendócrinos/metabolismo
5.
Molecules ; 29(10)2024 May 16.
Artigo em Inglês | MEDLINE | ID: mdl-38792203

RESUMO

Narrowband afterglow materials display interesting functions in high-quality anti-counterfeiting and multiplexed bioimaging. However, there is still a limited exploration of these afterglow materials, especially for those with a full width at half maxima (FWHM) around 30 nm. Here, we report the fabrication of narrowband organic/inorganic hybrid afterglow materials via energy transfer technology. Coronene (Cor) with a long phosphorescence feature and broad phosphorescence band is selected as the donor for energy transfer, and inorganic quantum dots (QDs) of CdSe/ZnS with a narrowband emission are used as acceptors. Upon doping into the organic matrix, the resultant three-component materials exhibit a narrowband afterglow with an afterglow lifetime of approximately 3.4 s and an FWHM of 31 nm. The afterglow wavelength of the afterglow materials can be controlled by the QDs. This work based on organic/inorganic hybrids provides a facile approach for developing multicolor and narrowband afterglow materials, as well as opens a new way for expanding the features of organic afterglow for multifunctional applications. It is expected to rely on narrowband afterglow emitters to solve the "spectrum congestion" problem of high-density information storage in optical anti-counterfeiting and information encryption.

6.
J Cell Mol Med ; 27(10): 1384-1397, 2023 05.
Artigo em Inglês | MEDLINE | ID: mdl-37005492

RESUMO

Human placental mesenchymal stem cells (PMSCs) can prevent liver ischaemia-reperfusion injury (LIRI). However, their therapeutic effects are limited. Therefore, additional research is required to elucidate the mechanisms of PMSC-mediated LIRI prevention and enhance the related therapeutic effects. This study aimed to examine the role of the Lin28 protein in the regulation of glucose metabolism in PMSCs. Further, it explored whether Lin28 could enhance the protective effects of PMSCs against LIRI and investigated the underlying mechanisms. Western blotting was performed to examine Lin28 expression in PMSCs under hypoxic conditions. A Lin28 overexpression construct was introduced into PMSCs, and the effect on glucose metabolism was examined using a glucose metabolism kit. Further, the expression of some proteins involved in glucose metabolism and the PI3K-AKT pathway and the levels of microRNA Let-7a-g were examined using western blots and real-time quantitative PCR, respectively. To examine the relationship between Lin28 and the PI3K-Akt pathway, the effects of AKT inhibitor treatment on the changes induced by Lin28 overexpression were examined. Subsequently, AML12 cells were co-cultured with PMSCs to elucidate the mechanisms via which PMSCs prevent hypoxic injury in liver cells in vitro. Finally, C57BL/6J mice were used to establish a partial warm ischaemia-reperfusion model. The mice received intravenous injections containing PMSCs (control and Lin28-overexpressing PMSCs). Finally, their serum transaminase levels and degree of liver injury were assessed using biochemical and histopathological methods, respectively. Lin28 was upregulated under hypoxic conditions in PMSCs. Lin28 exerted protective effects against hypoxia-induced cell proliferation. Moreover, it increased the glycolytic capacity of PMSCs, allowing PMSCs to produce more energy under hypoxic conditions. Lin28 also activated the PI3K-Akt signalling pathway under hypoxic conditions, and its effects were attenuated by AKT inhibition. Lin28 overexpression could protect cells against LIRI-induced liver damage, inflammation and apoptosis and could also attenuate hypoxia-induced hepatocyte injury. Lin28 enhances glucose metabolism under hypoxic conditions in PMSCs, thereby exerting protective effects against LIRI by activating the PI3K-Akt signalling pathway. Our study is the first to report the potential of genetically modified PMSCs for LIRI treatment.


Assuntos
Hepatopatias , Traumatismo por Reperfusão , Animais , Feminino , Humanos , Camundongos , Gravidez , Apoptose/genética , Glucose/farmacologia , Hipóxia , Camundongos Endogâmicos C57BL , Fosfatidilinositol 3-Quinases/genética , Fosfatidilinositol 3-Quinases/metabolismo , Placenta/metabolismo , Proteínas Proto-Oncogênicas c-akt/genética , Proteínas Proto-Oncogênicas c-akt/metabolismo , Traumatismo por Reperfusão/genética , Traumatismo por Reperfusão/prevenção & controle , Traumatismo por Reperfusão/metabolismo
7.
J Transl Med ; 21(1): 487, 2023 07 20.
Artigo em Inglês | MEDLINE | ID: mdl-37474946

RESUMO

BACKGROUND: Lipid metabolism plays an important role in liver regeneration, but its regulation still requires further research. In this study, lipid metabolites involved in mouse liver regeneration at different time points were sequenced and analyzed to study their influence on liver regeneration and its mechanism. METHODS: Our experiment was divided into two parts. The first part examined lipid metabolites during liver regeneration in mice. In this part, lipid metabolites were sequentially analyzed in the livers of 70% mouse hepatectomy models at 0, 1, 3and 7 days after operation to find the changes of lipid metabolites in the process of liver regeneration. We screened L-carnitine as our research object through metabolite detection. Therefore, in the second part, we analyzed the effects of carnitine on mouse liver regeneration and lipid metabolism during liver regeneration. We divided the mouse into four groups: control group (70% hepatectomy group); L-carnitine group (before operation) (L-carnitine were given before operation); L-carnitine group (after operation)(L-carnitine were given after operation) and L-carnitine + perhexiline maleate (before operation) group. Weighing was performed at 24 h, 36 and 48 h in each group, and oil red staining, HE staining and MPO staining were performed. Tunnel fluorescence staining, Ki67 staining and serological examination. RESULTS: Sequencing analysis of lipid metabolites in 70% of mouse livers at different time points after hepatectomy showed significant changes in carnitine metabolites. The results showed that, compared with the control group the mouse in L-carnitine group (before operation) at 3 time points, the number of fat drops in oil red staining was decreased, the number of Ki67 positive cells was increased, the number of MPO positive cells was decreased, the number of Tunnel fluorescence positive cells was decreased, and the liver weight was increased. Serum enzymes were decreased. Compared with control group, L-carnitine group (after operation) showed similar trends in all indexes at 36 and 48 h as L-carnitine group (before operation). L-carnitine + perhexiline maleate (before operation) group compared with control group, the number of fat drops increased, the number of Ki67 positive cells decreased, and the number of MPO positive cells increased at 3 time points. The number of Tunnel fluorescent positive cells increased and serum enzyme increased. However, both liver weights increased. CONCLUSION: L-carnitine can promote liver cell regeneration by promoting lipid metabolism and reduce aseptic inflammation caused by excessive lipid accumulation.


Assuntos
Hepatectomia , Regeneração Hepática , Camundongos , Animais , Regeneração Hepática/fisiologia , Metabolismo dos Lipídeos , Carnitina/farmacologia , Carnitina/metabolismo , Antígeno Ki-67/metabolismo , Fígado/metabolismo , Lipídeos
8.
Mikrochim Acta ; 190(6): 248, 2023 06 02.
Artigo em Inglês | MEDLINE | ID: mdl-37266700

RESUMO

The modification of EGFR aptamer (Apt 1) and HER2 aptamer (Apt 2) with gold nanoparticles (AuNPs) is reported to obtain probe I (Apt 1-AuNPs) and probe II (Apt 2-AuNPs). Taking Eca109, KYSE510, and KYSE150 cells as models, the sandwich scattering system of probe I-cell-probe II was formed by the recognition of tumor markers by the aptamer modified probe, and the resonance Rayleigh scattering (RRS) spectra were investigated. The results showed that the scattering system can be used to quantitatively detect the Eca109 cell lines in the range 5.0×10 to 5.0×105 cells·mL-1 with a detection limit of 15 cells· mL-1.The system can also detect the KYSE510 cell lines in a linear range of 5.0×10 to 5.0×105 cells·mL-1 with a detection limit of 18 cells·mL-1 and the KYSE150 cell lines in a linear range of 3.0×10 to 5.0×105 cells·mL-1 with a detection limit of 12 cells·mL-1. To demonstrate the potential application of the RRS method for real sample analysis, cells were spiked into blank serum samples at concentrations from 1.0×102 to 1.0×105 cells·mL-1. The recovery was between 97.0% and 102.3%, and the RSD was between 1.1% and 4.9%, confirming the feasibility of the proposed method for ESCC cell determination.


Assuntos
Aptâmeros de Nucleotídeos , Neoplasias Esofágicas , Nanopartículas Metálicas , Humanos , Ouro , Neoplasias Esofágicas/diagnóstico
9.
Nano Lett ; 22(15): 6166-6172, 2022 Aug 10.
Artigo em Inglês | MEDLINE | ID: mdl-35912475

RESUMO

Manipulating the exchange bias (EB) effect using an electronic gate is a significant goal in spintronics. The emergence of van der Waals (vdW) magnetic heterostructures has provided improved means to study interlayer magnetic coupling, but to date, these heterostructures have not exhibited electrical gate-controlled EB effects. Here, we report electrically controllable EB effects in a vdW heterostructure, FePS3-Fe5GeTe2. By applying a solid protonic gate, the EB effects were repeatably electrically tuned. The EB field reaches up to 23% of the coercivity and the blocking temperature ranges from 30 to 60 K under various gate-voltages. The proton intercalations not only tune the average magnetic exchange coupling but also change the antiferromagnetic configurations in the FePS3 layer. These result in a dramatic modulation of the total interface exchange coupling and the resultant EB effects. The study is a significant step toward vdW heterostructure-based magnetic logic for future low-energy electronics.

10.
Molecules ; 28(8)2023 Apr 14.
Artigo em Inglês | MEDLINE | ID: mdl-37110716

RESUMO

A significant portion of the protein in food waste will contaminate the water. The chitosan/modified ß-cyclodextrin (CS/ß-CDP) composite membranes were prepared for the adsorption of bovine serum albumin (BSA) in this work to solve the problem of poor adsorption protein performance and easy disintegration by a pure chitosan membrane. A thorough investigation was conducted into the effects of the preparation conditions (the mass ratio of CS and ß-CDP, preparation temperature, and glutaraldehyde addition) and adsorption conditions (temperature and pH) on the created CS/ß-CDP composite membrane. The physical and chemical properties of pure CS membrane and CS/ß-CDP composite membrane were investigated. The results showed that CS/ß-CDP composite membrane has better tensile strength, elongation at break, Young's modulus, contact angle properties, and lower swelling degree. The physicochemical and morphological attributes of composite membranes before and after the adsorption of BSA were characterized by SEM, FT-IR, and XRD. The results showed that the CS/ß-CDP composite membrane adsorbed BSA by both physical and chemical mechanisms, and the adsorption isotherm, kinetics, and thermodynamic experiments further confirmed its adsorption mechanism. As a result, the CS/ß-CDP composite membrane of absorbing BSA was successfully fabricated, demonstrating the potential application prospect in environmental protection.


Assuntos
Quitosana , Eliminação de Resíduos , Poluentes Químicos da Água , beta-Ciclodextrinas , Quitosana/química , Adsorção , Espectroscopia de Infravermelho com Transformada de Fourier , Alimentos , beta-Ciclodextrinas/química , Cinética , Soroalbumina Bovina , Concentração de Íons de Hidrogênio , Poluentes Químicos da Água/química
11.
J Am Chem Soc ; 144(11): 4942-4951, 2022 Mar 23.
Artigo em Inglês | MEDLINE | ID: mdl-35262357

RESUMO

Activation of O2 is a crucial step in oxidation processes. Here, the concept of sp-hybridized C≡C triple bonds as an electron donor is adopted to develop highly active and stable catalysts for molecular oxygen activation. We demonstrate that the neighboring sp-hybridized C and Cu sites on the interface of the sub-nanocluster CuO/graphdiyne are the key structures to effectively modulate the O2 activation process in the bridging adsorption mode. The as-prepared sub-nanocluster CuO/graphdiyne catalyst exhibited the highest CO oxidation activity and readily converted 50% CO at around 133 °C, which is 34 and 94 °C lower than that for CuO/graphene and CuO/active carbon catalysts, respectively. In situ diffused reflectance infrared Fourier transform spectroscopy and density functional theory calculation results proved that the neighboring sp-hybridized C is more favorable to promote the rapid dissociation of carbonate than sp2-hybridized C without overcoming any energy barrier. The gaseous CO directly reacts with the active molecular oxygen and tends to proceed through the E-R mechanism with a relatively low energy barrier (0.20 eV). This work revealed that sp-hybridized C of graphdiyne-based materials could effectively improve the O2 activation efficiency, which could facilitate the low-temperature oxidation processes.

12.
Anal Bioanal Chem ; 414(5): 1891-1898, 2022 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-35001192

RESUMO

Telomerase is considered a valuable diagnostic and prognostic cancer biomarker. Accurate and reliable detection of telomerase activity is of great value in clinical diagnosis, screening of inhibitors, and therapeutics. Here, we developed a novel amplified fluorescence resonance energy transfer (FRET) nanoprobe for highly sensitive and reliable monitoring of intracellular telomerase activity. The nanoprobe (QDSA@DNA) was composed of a streptavidin-modified quantum dot (QDSA) which was functionalized with a telomerase primer sequence (TP) and Cy5-tagged signal switching sequence (SS) through biotin-streptavidin interaction. When the nanoprobe was assembled, the Cy5 was in close proximity to the QDSA, resulting in high FRET efficiency from the QDSA to Cy5. In the presence of telomerase, the TP could be extended to produce telomeric repeat units, which was complementary to the loop of SS. Thus, the SS could hybridize with elongated sequences to form a rigid double-stranded structure, which forced the Cy5 away from the surface of the QDSA, causing low FRET efficiency. Furthermore, due to the production of multiple repeat units by telomerase, multiple hairpin structures could be opened, yielding significant fluorescence ratio (FQDsa/FCy5) enhancement for sensing of telomerase activity. In this way, the combination of a FRET and target-assisted strategy in a nanoprobe improved the detection accuracy and amplified the detection signal, respectively. The QDSA@DNA nanoprobe also showed high selectivity, excellent nuclease stability, and good biocompatibility. More importantly, this nanoprobe was found to be an excellent platform for efficient monitoring of intracellular telomerase activity, providing a potential platform in tumor diagnosis and screening of telomerase-related inhibitors.


Assuntos
Corantes Fluorescentes/química , Nanoestruturas/química , Telomerase/metabolismo , Transferência Ressonante de Energia de Fluorescência/métodos , Células HeLa , Humanos , Pontos Quânticos
13.
Nano Lett ; 21(13): 5599-5605, 2021 Jul 14.
Artigo em Inglês | MEDLINE | ID: mdl-34152781

RESUMO

Magnetic van der Waals (vdW) materials are poised to enable all-electrical control of magnetism in the two-dimensional limit. However, tuning the magnetic ground state in vdW itinerant ferromagnets by voltage-induced charge doping remains a significant challenge, due to the extremely large carrier densities in these materials. Here, by cleaving the vdW itinerant ferromagnet Fe5GeTe2 (F5GT) into 5.4 nm (around two unit cells), we find that the ferromagnetism (FM) in F5GT can be substantially tuned by the thickness. Moreover, by utilizing a solid protonic gate, an electron doping concentration of above 1021 cm-3 has been exhibited in F5GT nanosheets. Such a high carrier accumulation exceeds that possible in widely used electric double-layer transistors (EDLTs) and surpasses the intrinsic carrier density of F5GT. Importantly, it is accompanied by a magnetic phase transition from FM to antiferromagnetism (AFM). The realization of an antiferromagnetic phase in nanosheet F5GT suggests the promise of applications in high-temperature antiferromagnetic vdW devices and heterostructures.

14.
J Am Chem Soc ; 143(23): 8720-8730, 2021 Jun 16.
Artigo em Inglês | MEDLINE | ID: mdl-34100598

RESUMO

High-current density (≥1 A cm-2) is a critical factor for large-scale industrial application of water-splitting electrocatalysts, especially seawater-splitting. However, it still remains a great challenge to reach high-current density due to the lack of active and stable intrinsic catalytic active sites in catalysts. Herein, we report an original three-dimensional self-supporting graphdiyne/molybdenum oxide (GDY/MoO3) material for efficient hydrogen evolution reaction via a rational design of "sp C-O-Mo hybridization" on the interface. The "sp C-O-Mo hybridization" creates new intrinsic catalytic active sites (nonoxygen vacancy sites) and increases the amount of active sites (eight times higher than pure MoO3). The "sp C-O-Mo hybridization" facilitates charge transfer and boosts the dissociation process of H2O molecules, leading to outstanding HER activity with high-current density (>1.2 A cm-2) in alkaline electrolyte and a decent activity and stability in natural seawater. Our results show that high-current density electrocatalysts can be achieved by interfacial chemical bond engineering, three-dimensional structure design, and hydrophilicity optimization.

15.
IUBMB Life ; 73(1): 108-117, 2021 01.
Artigo em Inglês | MEDLINE | ID: mdl-33205540

RESUMO

Nasal-type natural killer/T-cell lymphoma (NKTCL) is an aggressive malignancy with poor survival outcomes that is relatively resistant to chemotherapy. N6-Methyladenosine (m6A) modification, the most prevalent modification of eukaryotic messenger RNA, is involved in the progression of various tumors. However, it is unclear whether it has a physiological role in NKTCL development. To address this question, we probed its function and molecular mechanisms in NKTCL. Initially, we demonstrated that Wilms' tumor 1-associated protein (WTAP), a major RNA N6-adenosine methyltransferase, was obviously upregulated in human NKTCL cell lines (YTS and SNK-6 cells), compared with normal NK cells. Functionally, depletion of WTAP noticeably repressed proliferation and facilitated apoptosis in YTS and SNK-6 cells. Moreover, intervention of WTAP evidently prohibited NKTCL cell chemotherapy resistance to cisplatin, as reflected by a lower inhibition of cell viability and decreased expression of drug resistance-associated protein expression MRP-1 and P-gp in YTS and SNK-6 cells. With regard to the mechanism, we revealed that WTAP enhanced dual-specificity phosphatases 6 (DUSP6) expression by increasing m6A levels of DUSP6 mRNA transcript, leading to oncogenic functions in NKTCL. Interestingly, WTAP contributed to the progression and chemotherapy sensitivity of NKTCL by stabilizing DUSP6 mRNA in an m6A-dependent manner. Taken together, these findings uncovered a critical function for WTAP-guided m6A methylation and identified DUSP6 as an important target of m6A modification in the regulation of chemotherapy resistance in NKTCL oncogenesis. This study highlights WTAP as a potential therapeutic target of NKTCL treatment.


Assuntos
Adenosina/análogos & derivados , Proteínas de Ciclo Celular/metabolismo , Cisplatino/farmacologia , Resistencia a Medicamentos Antineoplásicos , Fosfatase 6 de Especificidade Dupla/química , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Linfoma Extranodal de Células T-NK/patologia , Fatores de Processamento de RNA/metabolismo , Adenosina/química , Antineoplásicos/farmacologia , Apoptose , Biomarcadores Tumorais/genética , Biomarcadores Tumorais/metabolismo , Proteínas de Ciclo Celular/genética , Proliferação de Células , Fosfatase 6 de Especificidade Dupla/genética , Fosfatase 6 de Especificidade Dupla/metabolismo , Humanos , Linfoma Extranodal de Células T-NK/tratamento farmacológico , Linfoma Extranodal de Células T-NK/genética , Linfoma Extranodal de Células T-NK/metabolismo , Metilação , Neoplasias Nasais/tratamento farmacológico , Neoplasias Nasais/genética , Neoplasias Nasais/metabolismo , Neoplasias Nasais/patologia , Fatores de Processamento de RNA/genética , Células Tumorais Cultivadas
16.
Opt Lett ; 46(12): 2823, 2021 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-34129549

RESUMO

This publisher's note contains corrections to Opt. Lett.46, 2501 (2021)OPLEDP0146-959210.1364/OL.426833.

17.
Opt Lett ; 46(10): 2501-2504, 2021 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-33988619

RESUMO

Recently, the growing interest in few-mode fibers in telecommunications and high-power lasers has stimulated the demand for fiber mode decomposition (MD). Here we present a fast fiber MD method with a lensless fiber-point-diffraction interferometer. The complex amplitude at the fiber end is achieved by the polarization phase-shifting technique and the lensless imaging technique. Then, the eigenmode coefficients are determined by the mode orthogonal operations of the complex amplitude. In the experiment, the SMF-28e fiber containing 10 linear polarized modes at the wavelength of 632.8 nm is studied for MD. The decomposition of the 50 * 50 pixels interferograms takes only 0.0168 s. The similarity of the intensity patterns of the testing light is larger than 97% before and after the MD. This new, to the best of our knowledge, method can achieve fast and accurate 10-mode MD without using any imaging systems.

18.
Chemistry ; 27(42): 10898-10902, 2021 Jul 26.
Artigo em Inglês | MEDLINE | ID: mdl-33780036

RESUMO

Anthrathiadiazole is a key synthon for the construction of large azaacenes, however, the attachment of different substituents onto the skeleton of anthrathiadiazole is difficult but highly desirable because it could be easy to enrich the structures of azaacenes. Here, it is demonstrated that anthrathiadiazole derivatives with -Br, -CN, and -OCH3 groups could be easily constructed through a simple [4+2] cycloaddition reaction between a,a,a',a'-tetrabromo-o-xylenes derivatives and benzo[c][1,2,5]thiadiazole-4,7-dione. The structures of the as-prepared compounds with different substituents were carefully characterized. Moreover, the basic physical properties of the as-prepared anthrathiadiazole derivatives were fully investigated, where the cyano-substituted derivative (BTH-CN) has the highest stability and the methoxy-substituted derivative (BTH-OCH3 ) is easy to be oxidized. Moreover, the two-photon absorption (TPA) characteristics of different anthrathiadiazoles are also studied by using the femtosecond Z-scan technique. The results show that the fused anthrathiadiazole skeletons possess large TPA cross-section values δ2 in the range of 3000-5000 GM, where the nature, position and strength of the substituted groups have strong effect on these values.

19.
Angew Chem Int Ed Engl ; 60(38): 20795-20802, 2021 09 13.
Artigo em Inglês | MEDLINE | ID: mdl-33908155

RESUMO

Inspired by the unique characteristics of living cells, the creation of life-inspired functional ensembles is a rapidly expanding research topic, enabling transformative applications in various disciplines. Herein, we report a facile method for the fabrication of phospholipid and block copolymer hybrid bi-microcompartments via spontaneous asymmetric assembly at the water/tributyrin interface, whereby the temperature-mediated dewetting of the inner microcompartments allowed for exocytosis to occur in the constructed system. The exocytosis location and commencement time could be controlled by the buoyancy of the inner microcompartment and temperature, respectively. Furthermore, the constructed bi-microcompartments showed excellent biocompatibility and a universal loading capacity toward cargoes of widely ranging sizes; thus, the proliferation and temperature-programmed transportation of living organisms was achieved. Our results highlight opportunities for the development of complex mesoscale dynamic ensembles with life-inspired behaviors and provide a novel platform for on-demand transport of various living organisms.


Assuntos
Escherichia coli/metabolismo , Corantes Fluorescentes/metabolismo , Polímeros/metabolismo , Saccharomyces cerevisiae/metabolismo , Schizosaccharomyces/metabolismo , Temperatura , Sobrevivência Celular , Escherichia coli/citologia , Exocitose , Corantes Fluorescentes/química , Imagem Óptica , Tamanho da Partícula , Polímeros/síntese química , Polímeros/química , Saccharomyces cerevisiae/citologia , Schizosaccharomyces/citologia , Propriedades de Superfície
20.
Anal Chem ; 91(2): 1213-1216, 2019 01 15.
Artigo em Inglês | MEDLINE | ID: mdl-30565909

RESUMO

Intrinsic properties of nickel have enabled its wide applications as an effective catalyst. In this study, nickel nanowires (Ni NWs) as electron donors for oxidized cytochrome c (Cyt c) are investigated, which are NW diameter, temperature, and pH value-dependent. The reductive and magnetic properties facilitate the Ni NWs to rapidly and conveniently reduce Cyt c in complicated biological samples. Moreover, we find that the Ni NWs combined with resonance Raman spectroscopy have specificity toward Cyt c detection in real biological samples, which is successfully used to distinguish the redox state of the released Cyt c from isolated mitochondria in apoptotic Hela cells. Moreover, rapid label-free Cyt c quantification can be achieved by surface-enhanced Raman spectroscopy with a limit of detection of 1 nM and long concentration linear range (1 nM-1 µM). The proposed Ni NWs-based reduction approach will significantly simplify the traditional biological methods and has great potential in the application of Cyt c-related apoptotic studies.


Assuntos
Apoptose/fisiologia , Citocromos c/análise , Nanofios/química , Níquel/química , Citocromos c/química , Citocromos c/metabolismo , Células HeLa , Humanos , Limite de Detecção , Mitocôndrias/metabolismo , Oxirredução , Análise Espectral Raman/métodos
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