Microarray analysis of differentially expressed long non-coding RNAs in daidzein-treated lung cancer cells.

Daidzein has been found to significantly inhibit the proliferation of lung cancer cells, while its potential molecular mechanisms remain unclear. To determine the molecular mechanism of daidzein on lung cancer cells, the Capital Bio Technology Human long non-coding (lnc) RNA Array v4, 4×180K chip was used to detect the gene expression profiles of 40,000 lncRNAs and 34,000 mRNAs in a human cancer cell line. Reverse transcription-quantitative (RT-q) PCR analysis was performed to detect the expression levels of target lncRNA and mRNAs in the H1299 cells treated with and without daidzein, using the lncRNA and mRNA gene chip. Bioinformatics analysis was performed to determine the differentially expressed genes from the results of the chip assays. There were 119 and 40 differentially expressed lncRNAs and mRNAs, respectively, that had a 2-fold change in expression level. A total of eight lncRNAs were upregulated in the H1299 lung cancer cells, while 111 lncRNAs were downregulated. Furthermore, five mRNAs were upregulated, and 35 mRNAs were downregulated. A total of six differentially expressed lncRNAs (ENST00000608897.1, ENST00000444196.1, ENST00000608741.1, XR_242163.1, ENST00000505196.1 and ENST00000498032.1) were randomly selected to validate the microarray data, which were consistent with the RT-qPCR analysis results. Differentially expressed mRNAs were enriched in important Gene Ontology terms and Kyoto Encyclopedia of Genes and Genomes pathways. Taken together, the results of the present study demonstrated that daidzein affected the expression level of lncRNAs in lung cancer cells, suggesting that daidzein may have potential effects on lung cancer cells.

North Atlantic salinity as a predictor of Sahel rainfall.

Water evaporating from the ocean sustains precipitation on land. This ocean-to-land moisture transport leaves an imprint on sea surface salinity (SSS). Thus, the question arises of whether variations in SSS can provide insight into terrestrial precipitation. This study provides evidence that springtime SSS in the subtropical North Atlantic ocean can be used as a predictor of terrestrial precipitation during the subsequent summer monsoon in Africa. Specifically, increased springtime SSS in the central to eastern subtropical North Atlantic tends to be followed by above-normal monsoon-season precipitation in the African Sahel. In the spring, high SSS is associated with enhanced moisture flux divergence from the subtropical oceans, which converges over the African Sahel and helps to elevate local soil moisture content. From spring to the summer monsoon season, the initial water cycling signal is preserved, amplified, and manifested in excessive precipitation. According to our analysis of currently available soil moisture data sets, this 3-month delay is attributable to a positive coupling between soil moisture, moisture flux convergence, and precipitation in the Sahel. Because of the physical connection between salinity, ocean-to-land moisture transport, and local soil moisture feedback, seasonal forecasts of Sahel precipitation can be improved by incorporating SSS into prediction models. Thus, expanded monitoring of ocean salinity should contribute to more skillful predictions of precipitation in vulnerable subtropical regions, such as the Sahel.

Impact of the Interdecadal Pacific Oscillation on Tropical Cyclone Activity in the North Atlantic and Eastern North Pacific.

Tropical cyclones (TCs) are among the most devastating weather systems affecting the United States and Central America (USCA). Here we show that the Interdecadal Pacific Oscillation (IPO) strongly modulates TC activity over the North Atlantic (NA) and eastern North Pacific (eNP). During positive IPO phases, less (more) TCs were observed over NA (eNP), likely due to the presence of stronger (weaker) vertical wind shear and the resulting changes in genesis potential. Furthermore, TCs over NA tend to keep their tracks more eastward and recurve at lower latitudes during positive IPO phases. Such variations are largely determined by changes in steering flow instead of changes in genesis locations. Over the eNP, smaller track variations are observed at different IPO phases with stable, westward movements of TCs prevailing. These findings have substantial implications for understanding decadal to inter-decadal fluctuations in the risk of TC landfalls along USCA coasts.