Colorimetric Differentiation of Multiple Oxidizing Anions Based on Two Core-Shell Au@Ag Nanoparticles with Different Morphologies as Array Recognition Elements.

The efficient discrimination of oxidizing anions is of considerable importance in environmental monitoring. Here, for the first time, we have developed a simple and fast colorimetric sensor array for detection and identification of oxidizing anions, which takes advantage of the etching of the Ag shell of two core-shell Au@Ag nanoparticles (Au@Ag nanospheres (Au@Ag NPs) and Au@Ag nanocubes (Au@Ag NCs)) by oxidizing anions. The differential etching ability of various oxidizing anions to the Ag shell of the two Au@Ag nanoparticles resulted in different absorbance and color change of the nanoparticles. Thus, employing Au@Ag NPs and Au@Ag NCs as the array's receptors and the indicators, six oxidizing anions (i.e., BrO3-, Cr2O72-, ClO4-, IO3-, IO4-, and MnO4-) down to 10 nM could be identified from each other by their own colorimetric response patterns. Moreover, the complex mixtures of oxidizing anions could be well discriminated. Most importantly, the sensor array was successfully applied to the discrimination of oxidizing anions in river water and tap water samples.

ChIP-sequencing analysis of E2F transcription factor 2 reveals its role in various biological processes of rheumatoid arthritis synovial fibroblasts.

The development and progression of rheumatoid arthritis (RA) are complex and the pathogenesis of this disease is not fully understood. E2F transcription factor 2 (E2F2) affects the development and progression of many diseases. To identify the mechanisms underlying the role of E2F2 in RA, chromatin immunoprecipitation was performed followed by sequencing (ChIP-seq) using the E2F2 antibody. Gene Ontology (GO) analysis of differentially expressed genes (DEGs) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment of captured downstream target genes and Metascape analysis of 22 protein molecules partly elucidated the mechanism by which E2F2 affects the progression of RA. Results indicated that E2F2 affects the metabolism of RASFs and the development of ribosome synthesis as well as the stress response. Results indicated that E2F2 can affect multiple biological processes involving RASFs and indicate a unique possibility of targeting E2F2 in the treatment of RA.

The E2F transcription factor 2: What do we know?

E2F transcription factor 2 (E2F2) is a member of the E2F family of transcription factors. The classical view is that some E2Fs act as "activators" and others "inhibitors" of cell cycle gene expression. However, the so-called "activator" E2F2 is particularly enigmatic, with seemingly contradictory roles in the cell cycle, proliferation, apoptosis, inflammation, and cell migration and invasion. How can we rationalize the apparently opposing functions of E2F2 in different situations? This is difficult because different methods of studying E2F2 have yielded conflicting results, so extrapolating mechanisms from an observed endpoint is challenging. This review will attempt to summarize and clarify these issues. This review focuses on genetic studies that have helped elucidate the biological functions of E2F2 and that have enhanced our understanding of how E2F2 is integrated into pathways controlling the cell cycle, proliferation, apoptosis, inflammation, and cell migration and invasion. This review will also discuss the function of E2F2 in cancer and other diseases. This review provides a strong basis for further research on the biological function and clinical potential of E2F2.