Comparative analysis of MIR168 promoters in three plant species.

MicroRNAs (miRNAs) play important roles in the regulation of gene expression by post-transcriptionally targeting mRNAs for cleavage or translational repression. miR168 is a key miRNA because it regulates the expression of the slicer protein ARGONAUTE1 (AGO1), which catalyzes mRNA cleavage. Interestingly, plant miR168s are highly evolutionarily conserved; however, it is unclear whether MIR168 promoter elements and expression patterns are also conserved. Here, we isolated MIR168 promoters from monocot rice and dicot grape genomes. To determine the expression pattern, different promoters were fused to a beta-glucoronidase reporter gene and the resulting constructs were then transformed in Arabidopsis. The results revealed clear differences in the MIR168 promoter sequence of monocot and dicot plant species. Moreover, the pattern of MIR168 promoter expression differed between monocots and dicots. These results suggest that, unlike that of miR168, the MIR168 promoter is not conserved in monocots and dicots.

The effects of the BT/TAMUS 2032 cationic peptides on innate immunity and susceptibility of young chickens to extraintestinal Salmonella enterica serovar Enteritidis infection.

The BT/TAMUS 2032 (BT) cationic peptides are a group of related cationic peptides produced by a Gram-positive bacterium. Cationic amphiphilic peptides have been found to stimulate or prime the innate immune responses in mammals. The innate immune system of poultry is functionally inefficient during the first week post-hatch enabling pathogens such as Salmonella enterica serovar Enteritidis (SE) to invade and colonize the visceral organs of these immature birds. The objective of the present study was to evaluate the effect of BT as an immunostimulator of the innate immune response of young chickens. BT, provided as a feed additive at three different concentrations (12, 24, or 48 ppm) for 4 days post-hatch, significantly increased protection against SE organ invasion in a concentration-dependent manner. The functional efficiency of heterophils, the avian equivalent to mammalian neutrophils, isolated from chickens fed the BT rations at the three concentrations was significantly up-regulated when compared to heterophils isolated from chickens fed a control starter ration as determined with an array of functional assays. Phagocytosis, oxidative burst, and degranulation were all significantly increased in a concentration-dependent manner in heterophils isolated from chickens fed the BT diets. This is the first report of bacterial cationic peptides inducing the up-regulation of the avian innate immune response and providing protection against extraintestinal Salmonella infections. The significance of these data is that the orally delivered cationic peptides stimulate the innate response at a time of immunologic inefficiency and increased susceptibility to bacterial infections (first week post-hatch). Because of the nonspecific nature of the innate response, we speculate that BT given as a feed additive during the first week post-hatch could provide increased protection against a variety of bacterial pathogens.

[Methods of diagnosis and correction of endogenous intoxication in obstetrical suppurative-inflammatory diseases]. / Metody diagnostiki i korrektsii éndogennoi toksikatsii pri gnoino-vospalitel'nykh zabolevaniiakh v akusherskoi praktike.

Content of middle molecular mass peptides was studied in blood of patients with pyo-inflammatory diseases. The peptide fraction was detected exhibiting a membrano-tropic effect, which correlated with the rate of endogenous intoxication. A procedure of autotransfusion of UV-irradiated blood was used in the course of detoxication.

Inflammation-induced intestinal hyperemia in the rat: role of neutrophils.

Both experimental colitis and human inflammatory bowel disease are characterized by an increased colonic blood flow. The objective of this study was to define the role of neutrophils in the colonic hyperemia associated with acetic acid-induced colitis in rats. One, two, and five days after the acetic acid enema, the colon was separated into five segments. Regional blood flow to each segment was measured using the radioactive microsphere technique. Tissue-associated myeloperoxidase activity was used as an index of neutrophil infiltration. Rectal blood flow and myeloperoxidase activity increased progressively after the acetic acid enema. At 5 days there were 3.9- and 4.6-fold increases in myeloperoxidase activity and blood flow, respectively. Comparable changes were noted in all bowel segments. The results suggest a temporal relationship between colonic blood flow and the extent of neutrophil infiltration. To assess directly the role of circulating and infiltrated neutrophils as mediators of the colitis-induced hyperemia, animals were rendered neutropenic approximately 8 h before the enema and neutropenia was maintained for another 24 h. Neutropenia did not modify the colitis-induced intestinal hyperemia normally observed at 24 h. We conclude from these findings that vasoactive agents derived from neutrophils do not mediate the increased colonic blood flow in this model of ulcerative colitis.

Expectant management of severe preterm preeclampsia: is intrauterine growth restriction an indication for immediate delivery?

OBJECTIVE: Expectant management of severe preterm preeclampsia is gaining widespread acceptance in clinical practice. The objective of our study was 2-fold-to determine the frequency of fetal deterioration with expectant management of severe preterm preeclampsia and to evaluate whether the presence of intrauterine growth restriction on admission is associated with a shorter admission-to-delivery interval or more deliveries resulting from nonreassuring fetal status in comparison with pregnancies with preeclampsia but without intrauterine growth restriction. STUDY DESIGN: This was an observational study of women with singleton pregnancies at <34 completed weeks' gestation who were admitted to the hospital with the diagnosis of severe preeclampsia and managed expectantly. Fetal status on admission, admission-to-delivery interval, indication for delivery, and neonatal outcome were examined. RESULTS: Forty-seven women were studied during a 3-year period (1996-1999). Gestational age at admission was 29.8 +/- 2.6 weeks. The mean admission-to-delivery interval for the entire group was 6.0 +/- 5.1 days; in 42.5% delivery was for fetal indications. In comparison with the absence of intrauterine growth restriction, the presence of intrauterine growth restriction at admission resulted in a significantly shorter admission-to-delivery interval (3.1 +/- 2.1 vs 6.6 +/- 6.1 days; P <.05). Most fetuses with intrauterine growth restriction (85.7%) were delivered before 1 week. Although 57% of fetuses with intrauterine growth restriction were delivered for fetal indications, versus 39% of fetuses without intrauterine growth restriction, these rates were not found to be significantly different. Neonatal outcomes, as reflected by Apgar scores, number of admissions to and duration of stay in the neonatal intensive care unit, and neonatal mortality rates, were similar. CONCLUSION: Pregnancies complicated by severe preterm preeclampsia and the presence of intrauterine growth restriction at admission may not benefit from expectant management beyond the 48 hours needed for betamethasone to act. Furthermore, all patients may benefit from close fetal monitoring before delivery because of the high rate of intervention for deteriorating fetal status.

A novel MADS-box gene subfamily with a sister-group relationship to class B floral homeotic genes.

Class B floral homeotic genes specify the identity of petals and stamens during the development of angiosperm flowers. Recently, putative orthologs of these genes have been identified in different gymnosperms. Together, these genes constitute a clade, termed B genes. Here we report that diverse seed plants also contain members of a hitherto unknown sister clade of the B genes, termed B(sister) (B(s)) genes. We have isolated members of the B(s) clade from the gymnosperm Gnetum gnemon, the monocotyledonous angiosperm Zea mays and the eudicots Arabidopsis thaliana and Antirrhinum majus. In addition, MADS-box genes from the basal angiosperm Asarum europaeum and the eudicot Petunia hybrida were identified as B(s) genes. Comprehensive expression studies revealed that B(s) genes are mainly transcribed in female reproductive organs (ovules and carpel walls). This is in clear contrast to the B genes, which are predominantly expressed in male reproductive organs (and in angiosperm petals). Our data suggest that the B(s) genes played an important role during the evolution of the reproductive structures in seed plants. The establishment of distinct B and B(s) gene lineages after duplication of an ancestral gene may have accompanied the evolution of male microsporophylls and female megasporophylls 400-300 million years ago. During flower evolution, expression of B(s) genes diversified, but the focus of expression remained in female reproductive organs. Our findings imply that a clade of highly conserved close relatives of class B floral homeotic genes has been completely overlooked until recently and awaits further evaluation of its developmental and evolutionary importance. Electronic supplementary material to this paper can be obtained by using the Springer Link server located at http://dx.doi.org/10.1007/s00438-001-0615-8.