Small lidar ratio of dust aerosol observed by Raman-polarization lidar near desert sources.

Previous studies have shown that the lidar ratio has a significant influence on the retrieval of the aerosol extinction coefficient via the Fernald method, leading to a large uncertainty in the evaluation of dust radiative forcing. Here, we found that the lidar ratios of dust aerosol were only 18.16 ± 14.23sr, based on Raman-polarization lidar measurements in Dunhuang (94.6°E, 40.1°N) in April of 2022. These ratios are much smaller than other reported results (∼50 sr) for Asian dust. This finding is also confirmed by some previous results from lidar measurements under different conditions for dust aerosols. The particle depolarization ratio (PDR) at 532 nm and color ratio (CR, 1064 nm/532 nm) of dust aerosols are0.28 ± 0.013 and 0.5-0.6, respectively, indicating that extremely fine nonspherical particles exist. In addition, the dust extinction coefficients at 532 nm range from2 × 10-4 to 6 × 10-4m-1for such small lidar ratio particles. Combining lidar measurements and model simulation by the T-matrix method, we further reveal that the reason for this phenomenon is mainly due to the relatively small effective radius and weak light absorption of dust particles. Our study provides a new insight into the wide variation in the lidar ratio for dust aerosols, which helps to better explain the impacts of dust aerosols on the climate and environment.

Simulated depolarization ratios for dust and smoke at laser wavelengths: implications for lidar application.

Polarization measurements have been widely used to detect aerosol properties by remote sensing in recent decades. To better understand the polarization characteristics of aerosols by lidar, the numerically exact T-matrix method was used to simulate the depolarization ratio (DR) of dust and smoke aerosols at typical laser wavelengths in this study. The results show that the DRs of dust and smoke aerosols have obviously different spectral dependences. Moreover, the ratio of DRs at two wavelengths has an obvious linear relationship with the microphysical properties of aerosols, including aspect ratio, effective radius and complex refractive index. At short wavelengths, we can use it to invert the absorption characteristics of particles, further improving the detection ability of lidar. Comparing the simulation results of different channels, DR, (color ratio) CR and (lidar ratio) LR have a good logarithmic fitting relationship at 532 nm and 1064 nm, which helps to classify the aerosol types. On this basis, a new inversion algorithm, "1ß+1α+2δ", was presented. By this algorithm, the backscattering coefficient (ß), extinction coefficient (α), DR (δ) at 532 nm and 1064 nm can be used to expand the range of inversion and compare lidar data with different configurations to obtain more extensive optical characteristics of aerosols. Our study enhances the application of laser remote sensing in aerosol observations more accurately.

Generation of functional posterior spinal motor neurons from hPSCs-derived human spinal cord neural progenitor cells.

Spinal motor neurons deficiency results in a series of devastating disorders such as amyotrophic lateral sclerosis (ALS), spinal muscular atrophy (SMA) and spinal cord injury (SCI). These disorders are currently incurable, while human pluripotent stem cells (hPSCs)-derived spinal motor neurons are promising but suffered from inappropriate regional identity and functional immaturity for the study and treatment of posterior spinal cord related injuries. In this study, we have established human spinal cord neural progenitor cells (hSCNPCs) via hPSCs differentiated neuromesodermal progenitors (NMPs) and demonstrated the hSCNPCs can be continuously expanded up to 40 passages. hSCNPCs can be rapidly differentiated into posterior spinal motor neurons with high efficiency. The functional maturity has been examined in detail. Moreover, a co-culture scheme which is compatible for both neural and muscular differentiation is developed to mimic the neuromuscular junction (NMJ) formation in vitro. Together, these studies highlight the potential avenues for generating clinically relevant spinal motor neurons and modeling neuromuscular diseases through our defined hSCNPCs.