[Extract process of cyclic adenosinemonophosphate (cAMP) in Ziziphus jujuba].

OBJECTIVE: To study the extract process of Cyclic adenosinemonophosphate (cAMP) in Ziziphus jujuba. METHODS: CAMP was extracted with water, separated and purificated by ion exchange and silica gel G column chromatography. RESULTS: Thin layer chromatography (TLC) of the extracts and control cAMP showed the same Rf 0.75; The UV-absorption of the extracts and control cAMP had maximal absorb peak at 260 nm and the least absorb value at 230 nm; Infra-red spectrum of the extracts was indistinguishable from that of control cAMP; Retention time of the extracts by high-performance liquid chromatography (HPLC) determinate was 5.237 minute,unsettled impurity peak,content was 97%. Control cAMP was 5.350 minute,their retention time were of little difference. CONCLUSION: Chemistry structure of this extract with that of control cAMP is the same.

Neuritogenesis of herbal (+)- and (-)-syringaresinols separated by chiral HPLC in PC12h and Neuro2a cells.

Syringaresinol isolated from Epimedium koreanum NAKA1 and Magnolia officinalis REHD. et WILS. was subjected to optical resolution by chiral HPLC to give (+)- and (-)-enantiomers. The two syringaresinol enantiomers, as well as a mixture of their glucosides, showed dose-dependent neuritogenesis in a concentration range from 0.24 to 24 microM in PC12h cells.