RESUMO
Nine kinds of carbon dots (CDs) were synthesized by using fruits with different varieties as carbon sources; meanwhile, the fluorescence characteristics, quantum yield, and response ability to different metal ions and free radicals were systematically studied. These CDs showed similar excitation and emission spectral ranges (λex ≈ 345 nm, λem ≈ 435 nm), but very different fluorescence quantum yield (QY), in which orange and cantaloupe CDs have the highest QY around 0.25 and green plum CDs showed the lowest quantum yield around 0.1. Interestingly, the fluorescence of all of these CDs can be significantly quenched by hydroxyl radical (â¢OH) and iron ion (Fe3+); however, these CDs showed very different response characteristics to other metal ions (e.g., Co2+, Ni2+, Cu2+, Ce3+, Mn2+, Ag+, and Fe2+). Through in-depth analysis, we found some interesting patterns of the influence of carbon sources on the fluorescence characteristics of CDs. Finally, by using white pitaya CDs as fluorescence probe, we realized sensing of Fe3+ and â¢OH with limits of detection (LOD) of 19.4 µM and 0.7 µM, respectively. Moreover, the CDs were also capable for sensitive detection in immune cells and even in zebrafishes. Our work can provide valuable guidance for the rational design of functional CDs for biological applications.
Assuntos
Carbono , Pontos Quânticos , Animais , Frutas , Íons , Metais , Peixe-ZebraRESUMO
As one of the nanostructures with enzyme-like activity, nanozymes have recently attracted extensive attention for their biomedical applications, especially for bacterial disinfection treatment. Nanozymes with high peroxidase activity are considered to be excellent candidates for building bacterial disinfection systems (nanozyme-H2O2), in which the nanozyme will promote the generation of ROS to kill bacteria based on the decomposition of H2O2. According to this criterion, a cerium oxide nanoparticle (Nanoceria, CeO2, a classical nanozyme with high peroxidase activity)-based nanozyme-H2O2 system would be very efficient for bacterial disinfection. However, CeO2 is a nanozyme with multiple enzyme-like activities. In addition to high peroxidase activity, CeO2 nanozymes also possess high superoxide dismutase activity and antioxidant activity, which can act as a ROS scavenger. Considering the fact that CeO2 nanozymes have both the activity to promote ROS production and the opposite activity for ROS scavenging, it is worth exploring which activity will play the dominating role in the CeO2-H2O2 system, as well as whether it will protect bacteria or produce an antibacterial effect. In this work, we focused on this discussion to unveil the role of CeO2 in the CeO2-H2O2 system, so that it can provide valuable knowledge for the design of a nanozyme-H2O2-based antibacterial system.
Assuntos
Bactérias/efeitos dos fármacos , Cério/farmacologia , Peróxido de Hidrogênio/farmacologia , Nanopartículas/química , Nanoestruturas/química , Antibacterianos/farmacologia , Antioxidantes/farmacologia , Bactérias/metabolismo , Oxirredução/efeitos dos fármacos , Peroxidase/metabolismo , Espécies Reativas de Oxigênio/metabolismoRESUMO
Carbon fiber-reinforced polymer composites (CFRPs) have gained widespread usage due to their promising physiochemical properties, while this causes large amounts of waste CFRPs worldwide. In this study, carbon fibers were successfully recovered from waste CFRPs through the pyrolysis-oxidation method, and the recovered fibers were reused in remanufacturing the secondary generation CFRPs. Moreover, the individual and interactive effects of pyrolysis-oxidation recovering parameters on the mechanical strength of the resulting remanufactured CFRPs (reCFRPs) were investigated. The recovered carbon fibers displayed surface chemical structures similar to virgin fibers but with high contents of oxygen-containing bonds. The tensile strength retention (TSR) of the reCFRPs was primarily influenced by oxidation temperature. Notably, a higher oxidation temperature, especially exceeding 560 °C, amplified the impact of oxidation duration on the TSR value. Similarly, concerning interlaminar shear strength retention (ISSR), the oxidation stage had a more substantial effect compared to the pyrolysis stage. As the oxidation temperature increased from 500 °C to 600 °C, the ISSR value initially increased and then decreased, irrespective of variations in pyrolysis parameters. Additionally, through integrating the response surface methodology (RSM) analysis and multi-island genetic algorithm (MIGA) global optimization, three recovery strategies, along with the corresponding processing parameters, were proposed to meet diverse requirements. The conclusions could provide valuable insights for optimizing the recovery and reuse of carbon fibers from waste CFRPs.
Assuntos
Fibra de Carbono , Oxirredução , Pirólise , Reciclagem , Fibra de Carbono/química , Reciclagem/métodos , Resistência à Tração , Polímeros/química , Carbono/químicaRESUMO
Efficient and spatiotemporally controllable cleavage of deoxyribonucleic acid (DNA) is of great significance for both disease treatment (e.g. tumour, bacterial infection, etc) and molecular biology applications (e.g. gene editing). The recently developed light-induced cleavage strategy based on catalytic nanoparticles has been regarded as a promising strategy for DNA controllable cleavage. Although the regulation based on orthogonal light in biomedical applications holds more significant advantages than that based on single light, nanoparticle-mediated DNA cleavage based on orthogonal light has yet to be reported. In this article, for the first time, we demonstrated an orthogonal light-regulated nanosystem for efficient and spatiotemporal DNA cleavage. In this strategy, tungsten oxide (WO3) nanoparticles with photochromic properties were used as nano-antennae to convert the photoenergy from the orthogonal visible light (405 nm) and near-infrared light (808 nm) into chemical energy for DNA cleavage. We verified that only the orthogonal light can trigger high cleavage efficiency on different types of DNA. Moreover, such an orthogonal light-response nano-system can not only induce significant apoptosis of tumour cells, but also effectively eliminate bacterial biofilms.
Assuntos
Nanopartículas , Neoplasias , Humanos , Clivagem do DNA , Nanopartículas/química , Raios Infravermelhos , DNARESUMO
The development of biodegradable nanomaterials for near-infrared photothermal antibacterial is of great significance to improve the biosafety of nano-antibacterial strategies in clinical application. In this study, a new nano-antibacterial strategy was developed, in which a biodegradable charge-transfer nanocomplex acted as a high-efficiency near-infrared light-activatable photothermal nanoconvertor. The charge-transfer nanocomplex was synthesized through oxidation-induced self-assembly of 3,3',5,5'-tetramethylbenzidine molecules. This nanocomplex can efficiently convert light energy around 900 nm into heat energy, with a photothermal conversion efficiency of up to 30%. More importantly, the nanocomplex can spontaneously degrade under physiological conditions within 12 hours. Utilizing the photothermal effect of this nanocomplex, both Gram-positive bacteria and Gram-negative bacteria can be inactivated within 2 minutes. In addition, the inactivation mechanism was systematically discussed and the results indicated that the photothermal effect induced bacterial cell membrane damage was probably responsible for the antibacterial effect.