Simultaneous determination of 10 kinds of biogenic amines in rat plasma using high-performance liquid chromatography coupled with fluorescence detection.

We describe a simple, rapid, selective and sensitive HPLC method coupled with fluorescence detection for simultaneous determination of 10 kinds of biogenic amines (BAs: tryptamine, 2-phenethylamine, putrescine, cadaverine, histamine, 5-hydroxytryptamine, tyramine, spermidine, dopamine and spermine). BAs and IS were derivated with dansyl chloride. Fluorescence detection (λex /λem = 340/510 nm) was used. A satisfactory result for method validation was obtained. The assay was shown to be linear over the ranges 0.005-1.0 µg/mL for tryptamine, 2-phenethylamine and spermidine, 0.025-1.0 µg/mL for putrescine, 0.001-1.0 µg/mL for cadaverine, 0.25-20 µg/mL for histamine, 0.25-10 µg/mL for 5-hydroxytryptamine and dopamine, and 0.01-1.0 µg/mL for tyramine and spermine. The limits of detection and the limits of quantification were 0.3-75.0 ng/mL and 1.0-250.0 ng/mL, respectively. Relative standard deviations were ≤5.14% for intra-day and ≤6.58% for inter-day precision. The recoveries of BAs ranged from 79.11 to 114.26% after spiking standard solutions of BAs into a sample at three levels. Seven kinds of BAs were found in rat plasma, and the mean values of tryptamine, 2-phenethylamine, putrescine, cadaverine, histamine, spermidine and spermine determined were 52.72 ± 7.34, 11.45 ± 1.56, 162.56 ± 6.26, 312.75 ± 18.11, 1306.50 ± 116.16, 273.89 ± 26.41 and 41.51 ± 2.07 ng/mL, respectively.

The application effect of preventive nursing intervention in patients with traumatic flail chest: A retrospective study.

This retrospective study explores the intervention effect of preventive nursing on participants with traumatic flail chest caused by multiple rib fractures. In this study, 92 patients with multiple rib fractures from a certain hospital from July 2020 to June 2022 were chosen as the research subjects. They were divided into research group and a control group according to different nursing methods. The control group received routine care. The research group received preventive care on the basis of the control group. Then, questionnaire survey was collected to evaluate the quality of life, pain degree, psychological state, complications, and lung function recovery of patients before and after nursing. In the quality of life score, before nursing, the scores of the research group and the control group were between 52 and 69, and the difference among the participants in the experiment did not possess statistical significance (P > .05). After nursing, the scores of the research group were markedly superior to the control group, and all scores possessed statistical significance (P < .05). In the pain score, the pain scores of the research group and the control group before preventive nursing were 7.87 ±â€…0.86 and 7.68 ±â€…0.75, respectively, and the difference among the participants in the experiment did not possess statistical significance (P > .05). After 6 months of nursing, the pain scores of the participants in the experiment were 2.84 ±â€…0.69 and 3.04 ±â€…0.72, respectively. The difference among the participants in the experiment possessed statistical significance (P < .05). In the Hamilton Anxiety Scale, the psychological state scores of the experimental group and the control group before intervention nursing were 52.87 ±â€…4.97 and 51.74 ±â€…4.56, respectively, and the scores after intervention nursing were 22.68 ±â€…4.69 and 36.87 ±â€…5.21, respectively. The difference among the participants in the experiment possessed statistical significance (P < .05). Preventive nursing interventions can improve long-term quality of life, significantly alleviate postoperative pain, decrease the incidence of postoperative complications, as well as enhance nursing satisfaction in patients with traumatic flail chest.

Impact of Abiotic Stress on Rice and the Role of DNA Methylation in Stress Response Mechanisms.

With the intensification of global climate change and the increasing complexity of agricultural environments, the improvement of rice stress tolerance is an important focus of current breeding research. This review summarizes the current knowledge on the impact of various abiotic stresses on rice and the associated epigenetic responses (DNA methylation). Abiotic stress factors, including high temperature, drought, cold, heavy metal pollution, and high salinity, have a negative impact on crop productivity. Epigenetic changes are key regulatory factors in plant stress responses, and DNA methylation is one of the earliest discovered and thoroughly studied mechanisms in these epigenetic regulatory mechanisms. The normal growth of rice is highly dependent on the environment, and changes in the environment can lead to rice sterility and severe yield loss. Changes in the regulation of the DNA methylation pathway are involved in rice's response to stress. Various DNA methylation-regulating protein complexes that function during rice development have been identified. Significant changes in DNA methylation occur in numerous stress-responsive genes, particularly those in the abscisic acid signaling pathway. These findings underscore the complex mechanisms of the abiotic stress response in rice. We propose the effective improvement of tolerance traits by regulating the epigenetic status of rice and emphasize the role of DNA methylation in abiotic stress tolerance, thereby addressing global climate change and ensuring food security.

Decreasing operating potential for water electrolysis to hydrogen via local confinement of iron-based soft coordination suprapolymers.

Currently there is intense interest in decreasing the operating potential for hydrogen evolution in water electrolysis to considerably decrease the energy cost. In this work we report a significant decrease of the operating potential for hydrogen evolution from neutral water mediated by an iron based soft coordination polymer (Fe(III)-SCSP). The creation of a local acidic environment with a thickness in the range of ~40 nm on the surface of a glassy carbon electrode allows enrichment of H(+) on the GCE, so that the operating potentials were effectively decreased. This strategy thus generates a new paradigm for lowering the operating potential of hydrogen generation from neutral water without the use of additional acids and organic cosolvents.

Blood Purification for Hypertriglyceridemia-Induced Acute Pancreatitis: A Meta-analysis.

OBJECTIVES: The purpose of our study is to investigate the efficacy and safety of blood purification (BP) therapy in hypertriglyceridemia-induced acute pancreatitis. METHODS: We searched PubMed, Embase, Cochrane Library, and Web of Science databases for articles published. RESULTS: The analysis included 13 studies with 934 patients (263 in BP group, 671 in control group). There was no difference in efficacy and safety between the BP group and the control group (all P > 0.05). Compared with conventional treatment, BP had shorter hospital stay (mean difference, -4.96; 95% confidence interval [CI], -8.81 to -1.11; P = 0.01) in the case of similar mortality and complications. Meanwhile, insulin treatment showed similar mortality to BP, but fewer local complications (odds risk, 2.18; 95% CI, 1.13-4.20; P = 0.02) and shorter hospital stay (mean difference, 5.46; 95% CI, 0.64-10.29; P = 0.03). CONCLUSIONS: In the treatment of hypertriglyceridemia-induced acute pancreatitis, BP methods are effective in accelerating triglyceride level reduction and shortening hospital stay but do not affect the efficacy or reduce mortality significantly compared with conventional treatment. Insulin therapy has the same effect as BP but decreases incidence of complications and cost.

Endovascular therapy versus intravenous thrombolysis in cervical artery dissection-related ischemic stroke: a meta-analysis.

BACKGROUND: The purpose of our meta-analysis is to evaluate the endovascular therapy (EVT) in patients with cervical artery dissection (CAD)-related acute ischemic stroke (AIS) by comparing its efficacy and safety with the ones of intravenous thrombolysis (IVT). METHODS: A systematic search on EVT to CAD-related ischemic stroke is performed. The meta-analysis models are applied to calculate either the risk ratio (RR) with 95% confidence interval (CI) or pooled proportions with 95% CI of favorable functional outcome (mRS = 0-2), excellent functional outcome (mRS = 0-1), symptomatic intracranial hemorrhage (SICH), mortality and recurrent stroke between EVT and IVT in CAD-related stroke. The differences between the two treatment groups are analyzed by the pooled odds ratio value and Chi-squared test. RESULTS: A total of 190 patients given EVT and 139 IVT-alone patients are included. By comparing EVT alone and IVT alone, patients treated with EVT alone are more likely to experience favorable outcomes than those treated with IVT alone (71.2% vs 53.4%). Besides, there is no significant difference in excellent functional outcome, SICH, mortality and recurrent stroke between the EVT-alone and IVT-alone groups (all P > 0.05). Towards general EVT (EVT with or without IVT), the outcomes are not significantly different from those of IVT alone except for a higher mortality rate (10.2% vs 3.2%). CONCLUSION: Based on our findings, EVT is considered to be more efficacious than IVT for CAD-related AIS patients. Although EVT alone tends to be safe and promising, its safety needs to be further evaluated, particularly for EVT separating from IVT therapy.

A Highly Selective and Sensitive Colorimetric Probe for Cu2+ Determination in Aqueous Media Based on Derivative of Tryptanthrin.

A new colorimetric probe, based on tryptanthrin derivative (TR-A), has been successfully synthesized. The probe shows good selectivity and sensitivity for Cu2+ over 12 competing metal ions in a 10 mM Tris-HCl buffer solution (pH 5.5). A significant peak at 623nm appears in the UV-Vis absorption of TR-A-Cu2+, and a noteworthy color change is observed with the naked eye from aquamarine blue to light orange. The interaction of TR-A and Cu2+ are proven to form a 1:1 binding stoichiometry; this identifying is expected to be completed within 1 min. The probe with a limit of detection (16 nM, R2 = 0.9934) shows excellent potential to determine Cu2+ in analysis systems.

A Thiophene-Containing Conductive Metallopolymer Using an Fe(II) Bis(terpyridine) Core for Electrochromic Materials.

Three Fe(II) bis(terpyridine)-based complexes with thiophene (Fe(L1)2), bithiophene (Fe(L2)2), and 3,4-ethylenedioxythiophene (Fe(L3)2) side chains were designed and synthesized for the purpose of providing two terminal active sites for electrochemical polymerization. The corresponding metallopolymers (poly-Fe(Ln)2, n = 2 or 3) were synthesized on indium tin oxide (ITO)-coated glass substrates via oxidative electropolymerization of the thiophene-substituted monomers and characterized using electrochemistry, X-ray photoelectron spectroscopy, UV-vis spectroscopy, and atomic force microscopy. The film poly-Fe(L2)2 was further studied for electrochromic (EC) color-switching properties and fabricated into a solid-state EC device. Poly-Fe(L2)2 films exhibit an intense MLCT absorption band at 596 nm (ε = 4.7 × 104 M-1 cm-1) in the UV-vis spectra without any applied voltage. Upon application of low potentials (between 1.1 and 0.4 V vs Fc+/Fc), the obtained electropolymerized film exhibited great contrast with a change of transmittance percentage (ΔT%) of 40% and a high coloration efficiency of 3823 cm2 C-1 with a switching time of 1 s. The film demonstrates commonplace stability and reversibility with a 10% loss in peak current intensity after 200 cyclic voltammetry cycles and almost no loss in change of transmittance (ΔT%) after 900 potential switches between 1.1 and 0.4 V (vs Fc+/Fc) with a time interval of 0.75 s. The electropolymerization of Fe(L2)2 provides convenient and controllable film fabrication. Electrochromic behavior was also achieved in a solid-state device composed of a poly-Fe(L2)2 film and a polymer-supported electrolyte sandwiched between two ITO-coated glass electrodes.