On-Demand Maneuvering of Diverse Prodrug Liquids on a Light-Responsive Candle-Soot-Hybridized Lubricant-Infused Slippery Surface for Highly Effective Toxicity Screening.

At present, microscale high-throughput screening (HTS) for drug toxicity has drawn increased attention. Reported methods are often constrained by the inability to execute rapid fusion over diverse droplets or the inflexibility of relying on rigid customized templates. Herein, a light-responsive candle-soot-hybridized lubricant-infused slippery surface (CS-LISS) was reported by one-step femtosecond laser cross-scanning to realize highly effective and flexible drug HTS. Due to its low-hysteresis merits, the CS-LISS can readily steer diverse droplets toward arbitrary directions at a velocity over 1.0 mm/s with the help of tracing lateral near-infrared irradiation; additionally, it has the capability of self-cleaning and self-deicing. Significantly, by integrating the CS-LISS with a GFP HeLa cell chip, high-efficiency drug toxicity screening can be successfully achieved with the aid of fluorescence imaging. This work provides insights into the design of microscale high-throughput drug screening.

Bioleaching of heavy metals from pig manure with indigenous sulfur-oxidizing bacteria: effects of sulfur concentration.

The purpose of this work was to study the sulfur concentration on bioleaching of heavy metals from pig manure employing indigenous sulfur-oxidizing bacteria. Also, the variations in physicochemical properties of pig manure before and after bioleaching were investigated. The results showed that sulfur concentration significantly affected the rate of acidification, sulfate production and metal solubilization during pig manure bioleaching process. A Michaelis-Menten type equation was utilized to interpret the relationships between sulfur concentration, sulfate production and metal solubilization in the bioleaching process. The rates of metal solubilization during pig manure bioleaching were well described by a first order kinetic equation related to time. After 12 days of bioleaching, 93%-97% of Zn, 96%-98% of Mn and 48%-94% of Cu were leached out from pig manure, respectively. The metals remaining in the pig manure residual were mainly existed in stable forms. In addition, elemental analysis showed that bioleaching process could significantly modify the dewaterbility and organic composition of pig manure. However, fertility analysis found that 9.0%-19.1% of nitrogen, 68.5%-71.0% of phosphorus, 76.5%-78.8% of potassium and 47.5%-49.4% of the total organic carbon (TOC) were lost from pig manure in the bioleaching process. Therefore, bioleaching process used in this study could be applied to remove heavy metals effectively from the pig manure, but more detailed studies need to be done to decrease the nutrients loss from pig manure.

Biochar addition for accelerating bioleaching of heavy metals from swine manure and reserving the nutrients.

Biochar was applied during the bioleaching of heavy metals (HMs) from swine manure (SM), in an attempt to accelerate the HMs removal rates and to reduce the losses of nutrient elements (nitrogen and phosphorus). Results showed that the addition of biochar (5gL-1) could not only significantly shorten the leaching time of HMs (Cu, Zn, Mn and Cd) from 10 (control) to 7days with a high solubilization efficiency of 90%, but also decrease the total nitrogen loss efficiency by 42.7% from 180.3 (control) to 103.3mgL-1 in the leachate. In addition, biochar addition facilitated Fe2+ oxidation rate, achieving much better pH and ORP conditions. Electronic conductivity and adsorption properties of biochar with changed microbial community probably contributed a lot to the enhanced HMs solubilization and reduced nitrogen loss during bioleaching. Although the addition of biochar only slightly reduced the total amount of phosphorus loss, the bioavailable phosphorus in SM after bioleaching was markedly increased by 13.7%.