[Relationship between the biological behavior of nasal cavity or sinonasal inverted papilloma and infection of human papillomavirus].

OBJECTIVE: To investigate the relationship between the prognosis of nasal inverted papilloma (NIP) and infection of human papillomavirus (HPV). METHODS: The paraffin-embedded tissue slides of 67 cases of NIP in nasal cavity or paranasal sinuses and of 10 cases of benign nasal polyps (as controls) underwent HE staining and in hybridization in situ (HIS) to detect the expression of 28 types of HPV and positive sites thereof. The 67 NIP patients, 45 males and 12 females, with the onset age of 55.7 (36 - 84), were divided into 3 groups according to the data of follow-up: non-recurrence group (n = 33, Group 1), recurrence group (n = 27, Group 2), and NIP with squamous cell carcinoma (n = 7, Group 3). RESULTS: The total HPV infection rate of the NIP slides was 49.25%, significantly higher than that of the control group (10%). The infection rates of HPV, especially the infection rates of HPV of the type 16/18, were significantly higher in Groups 2 and 3 than in Group 1. The infection rate of HPV of the type 16/18 was significantly higher in Group 3 than in other groups (all P < 0.01). The infected cells were located in the surface or upper part of the epithelial cell layer in Group 1, and in all parts of the epithelial cell layer in Group 3. CONCLUSION: the recurrence and malignant transition of NIP are related to HPV infection which may be attributed to the wider range of infected cells in these cases. The higher infection rate of high risk HPV type is one of the reasons for malignant transition.

[A clinicopathological study of 16 autopsy cases of anthracosilicosis with lung cancer].

OBJECTIVE: To investigate the clinicopathological characteristics of anthracosilicosis complicated with lung cancer. METHODS: Tissue specimens from 16 autopsy cases of 0(+) anthracosilicosis complicated with lung cancer were retrospectively studied by hematoxylin-eosin, histochemical, and immunohistochemical staining. RESULTS: All of 16 patients were male. The patient's age ranged from 46 to 57 years (average: 52.8 years). The dust-exposure time were over 25 years. The pneumoconiosis and dust fibrosis of different degrees in the lung were found. The positive detected rate of coal silicotic nodules was 93.75% (15/16). Among 16 cases of lung cancer, there were 5 cases of squamous cell carcinoma, and 5 cases of small cell undifferentiated carcinoma, 3 cases of bronchioloalveolar carcinoma, 2 cases of adenocarcinoma and 1 case of adenosquamous carcinoma. The typical pathological changes of anthracosilicosis complicated with lung cancer were: the cancer tissue was located at the side of coal dust fibrous focus and fibrosis lesion, or mixte with silicotic lesion. CK, EMA and CEA were positively expressed in most of the tumor cells, while vimentin was positive in the fibrocyte of dust fibrosis. CONCLUSIONS: The occurrence of some lung cancer may be related with fibrosis. The dust-exposed workers can suffer from lung cancer which is histologically identical to the general lung tumor. PCNA and Ki67 may be a prognostic index for anthracosilicosis with lung cancer, while vimentin may be a marker for the examination of dust fibrosis in anthracosilicosis.