Structure-Oxidative Metabolism Relationships of Substituted Flavones by Aspergillus niger.

Aspergillus niger is a rich source of oxidative enzymes, which are important for many industrial applications. However, systematic evaluation of their metabolic characteristics is limited. In this study, structure-dependent metabolism of flavones by Aspergillus niger were investigated with synthetic substrates. Metabolic inhibitor studies suggested that cytochrome P450s are the major enzymes in oxidative metabolism. The reactions include ring hydroxylation, O-demethylation, sulfone/sulfoxide formation, and oxidation of alkyls to carboxy groups. Initial oxidative metabolism occurred almost exclusively at 4'-substituents. 4'-Halogenated- and 3',5'-dihalogenated analogues were stable against biodegradation. Hydrophilic flavones were more rapidly metabolized than lipophilic analogues. Molecular widths of the A and B ring were important determinants of the position of metabolic oxidation and biotransformation rate. The structure-metabolism relationship analysis indicates that the shape of the B ring was the most important parameter of biotransformation. The electrostatic environment of the same ring also affected the transformation. Additionally, the results showed that the B ring may preferentially be oriented toward the catalytic center.

Feet distance and static postural balance: implication on the role of natural stance.

The purpose of this study was to investigate 1) the effect of feet distance on static postural balance and 2) the location of natural feet distance and its possible role in the relationship of feet distance and postural balance. Static balance tests were performed on a force platform for 100 s with six different feet distances (0, 5, 10, 15, 20, 25 cm). Measures of postural balance included mean amplitude of horizontal ground reaction force (GRF) as well as the mean distance and velocity of the center of pressure (COP). All measures were discomposed into anterioposterior and mediolateral directions. ANOVA and post-hoc comparison were performed for all measures with feet distance as an independent factor. Also measured was the feet distance at the natural stance preferred by each subject. All measures significantly varied with feet distance (p<0.001). Mean distance of COP showed monotonic decrease with feet distance. Mean amplitude of horizontal GRF as well as mean velocity of COP showed U-shaped pattern (decrease followed by increase) with the minimum at the feet distance of 15 cm or 20 cm, near which the natural feet distance of 16.5 (SD 3.8) cm was located. COP is regarded to be an approximation of the center of mass (hence the resultant performance of postural control) in an inverted pendulum model with the horizontal GRF ignored. On the other hand, horizontal GRF is the direct cause of horizontal acceleration of a center of mass. The present result on horizontal GRF shows that the effort of postural control is minimized around the feet distance of natural standing and implies why the natural stance is preferred.

Effects of nonlinearity in the materials used for the semi-rigid pedicle screw systems on biomechanical behaviors of the lumbar spine after surgery.

Recently, various types of semi-rigid pedicle screw fixation systems have been developed for the surgical treatment of the lumbar spine. They were introduced to address the adverse issues commonly found in traditional rigid spinal fusion--abnormally large motion at the adjacent level and subsequent degeneration. The semi-rigid system uses more compliant materials (nitinol or polymers) and/or changes in rod design (coiled or twisted rods) as compared to the conventional rigid straight rods made of Ti alloys (E = 114 GPa, υ = 0.32). However, biomechanical studies on the semi-rigid pedicle screw systems were usually limited to linear modeling of the implant and anatomic elements, which may not be capable of reflecting realistic post-operative motions of the spine. In this study, we evaluated the effects of nonlinearity in materials used for semi-rigid pedicle screw fixation systems to evaluate the changes in biomechanical behaviors using finite element analysis. Changes in range of motion (ROM) and center of rotation (COR) were assessed at the operated and adjacent levels. Actual load-displacement results of the semi-rigid rod from mechanical test were carried out to reflect the nonlinearity of the implant. In addition, nonlinear material properties of various spinal ligaments studies were used for the finite element modeling. The post-operative models were constructed by modifying the previously validated intact model of the L1-S1 spine. Eight different post-operative models were made to address the effects of nonlinearity-with a traditional stiffness modulus rod (with linear ligaments, case 1; with nonlinear ligaments, case 5), with a rigid rod (with linear ligaments, case 2; with nonlinear ligaments, case 6), with a soft rod (with linear ligaments, case 3; with nonlinear ligaments, case 7), and with a nonlinear rod (with linear ligaments, case 4; with nonlinear ligaments, case 8). To simulate the load on the lumbar spine in a neutral posture, follower load (400 N) was applied and then the hybrid loading condition was applied to measure the ROM and COR in the sagittal plane. The more the nonlinearity was included in the model the closer the motion behavior of the device was to that of the intact spine. Furthermore, our results showed that the nonlinearity of the semi-rigid rod was a more sensitive factor than the nonlinearity of the spinal ligaments on biomechanical behavior of the lumbar spine after surgery. Therefore, for better understanding of the surgical effectiveness of the spinal device, more realistic material properties such as nonlinearity of the device and anatomic elements should be considered. In particular, the nonlinear properties of the semi-rigid rod were considered more than the nonlinearity of spinal ligaments.