RESUMO
A novel human CC chemokine complementary DNA was identified in a library constructed from human fetal RNA, cloned into a baculovirus vector, and expressed in Sf9 insect cells. The mature recombinant protein that was released had the NH2-terminal sequence pyro-QPDALNVPSTC...and consisted of 75 amino acids. Minor amounts of two variants of 77 and 82 residues (NH2 termini: LAQPDA...and FNPQGLAQPDA...) were released as well. The novel chemokine was designated monocyte chemotactic protein 4 (MCP-4) and the variants were designated (LA)MCP-4 and (FNPQGLA)MCP-4. MCP-4 shares the pyroglutamic acidproline NH2-terminal motif and 56-61% sequence identity with the three known monocyte chemotactic proteins and is 60% identical to eotaxin. It has marked functional similarities to MCP-3 and eotaxin. Like MCP-3, MCP-4 is a chemoattractant of high efficacy for monocytes and T lymphocytes. On these cells, it binds to receptors that recognize MCP-1, MCP-3, and RANTES. On eosinophils, MCP-4 has similar efficacy and potency as MCP-3, RANTES, and cotaxin. It shares receptors with eotaxin and shows full cross-desensitization with this cosinophil-selective chemokine. Of the two variants, only (LA)MCP-4 could be purified in sufficient quantities for testing and was found to be at least 30-fold less potent than MCP-4 itself. This suggests that the 75-residue form with the characteristic NH2 terminus of an MCP is the biologically relevant species.
Assuntos
Quimiocinas CC , Quimiotaxia de Leucócito , Citocinas/química , Leucócitos/fisiologia , Proteínas Quimioatraentes de Monócitos/biossíntese , Proteínas Quimioatraentes de Monócitos/química , Proteínas Quimioatraentes de Monócitos/farmacologia , Acetilglucosaminidase/sangue , Sequência de Aminoácidos , Sequência de Bases , Linfócitos T CD4-Positivos/fisiologia , Linfócitos T CD8-Positivos/fisiologia , Quimiocina CCL11 , Quimiocina CCL7 , Quimiocinas/farmacologia , Clonagem Molecular , Citocinas/farmacologia , Primers do DNA , DNA Complementar , Feto , Biblioteca Gênica , Humanos , Técnicas In Vitro , Cinética , Leucócitos/efeitos dos fármacos , Dados de Sequência Molecular , Monócitos/fisiologia , Neutrófilos/fisiologia , Fragmentos de Peptídeos/química , Fragmentos de Peptídeos/isolamento & purificação , Reação em Cadeia da Polimerase , Homologia de Sequência de AminoácidosRESUMO
Abdominal cocoon is a rare cause of intestinal obstruction in adults. Diagnosis is usually established at laparotomy in patients with recurrent attacks of non-strangulating small bowel obstruction. A 40-year-old infertile Brazilian woman with intestinal obstruction and massive haemoserous ascites, due to coexistent ovarian endometriosis and abdominal cocoon, is reported. Abdominal pain, nausea, vomiting and a palpable mass, in addition to imaging of small bowel obstruction and thickened peritoneum, raised diagnostic suspicion. Higher awareness allows for early diagnoses and yields better results during management.