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1.
Cancer Cell Int ; 20: 57, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32099530

RESUMO

BACKGROUND: Abnormally expressed circular RNAs (circRNAs) are implicated in the development and treatment of gastric cancer (GC). Previous study has reported that hsa_circ_0003159 is expressed in GC. However, the role and mechanism of hsa_circ_0003159 in GC progression remain unclear. METHODS: GC tissues and normal tissues were harvested from 55 patients in this study. The levels of hsa_circ_0003159, microRNA (miR)-223-3p and N-myc downstream regulated gene 1 (NDRG1) were measured by quantitative real-time polymerase chain reaction or western blot. Cell proliferation, migration, invasion and apoptosis were determined by cell counting kit (CCK)-8, transwell assay, flow cytometry and western blot, respectively. The target association of miR-223-3p-hsa_circ_0003159 and miR-223-3p-NDRG1 was explored by dual-luciferase reporter assay. Xenograft model was established to assess the roles of hsa_circ_0003159 in GC in vivo. RESULTS: Hsa_circ_0003159 was lowly expressed in GC tissues and cells and mainly presented in the cytoplasm. Low expression of hsa_circ_0003159 was associated with lower overall survival and disease-free survival. Hsa_circ_0003159 overexpression inhibited proliferation, migration and invasion but induced apoptosis in GC cells. MiR-223-3p was a target of hsa_circ_0003159 and abated the effect of hsa_circ_0003159 on proliferation, migration, invasion and apoptosis in GC cells. Hsa_circ_0003159 promoted NDRG1 expression by competitively sponging miR-223-3p. Knockdown of NDRG1 reversed the suppressive effect of hsa_circ_0003159 on GC progression. Besides, hsa_circ_0003159 decreased GC cell xenograft tumor growth by regulating miR-223-3p and NDRG1. CONCLUSION: Hsa_circ_0003159 suppressed proliferation, migration, invasion and xenograft tumor growth but promoted apoptosis by decreasing miR-223-3p and increasing NDRG1 in GC, indicating a novel target for treatment of GC.

2.
Dis Colon Rectum ; 59(1): 70-78, 2016 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-26651115

RESUMO

BACKGROUND: The discussion on the role of mechanical bowel preparation and oral antibiotics in elective colorectal surgery is still ongoing. OBJECTIVE: This meta-analysis aimed to determine whether oral systemic antibiotics with mechanical bowel preparation are superior to systemic antibiotics and mechanical bowel preparation for prophylaxis of bacterial infection during elective colorectal operation. DATA SOURCES: Embase, PubMed, and the Cochrane Library were searched using the terms oral, antibiotics/antimicrobial, colorectal/rectal/colon/rectum, and surgery/operation. STUDY SELECTION: All of the available randomized controlled trials that compared the efficacy of combined oral and systemic antibiotics and mechanical bowel preparation with systemic antibiotics alone and mechanical bowel preparation in colorectal surgery and defined surgical site infection based on Centers for Disease Control and Prevention criteria were included. INTERVENTION: All of the statistical analyses were performed using Review Manager 5.2 software. A fixed model was used if there was no evidence of heterogeneity; otherwise, a random-effects model was used. MAIN OUTCOME MEASURES: We focused on incidence of surgical site infection among the groups. RESULTS: Seven randomized controlled trials that consisted of 1769 cases were eligible for analysis. We found that both total surgical site infection and incisional surgical site infection were significantly reduced in patients who received oral systemic antibiotics and mechanical bowel preparation compared with patients who received systemic antibiotics alone and mechanical bowel preparation (total: 7.2% vs 16.0%, p < 0.00001; incisional: 4.6% vs 12.1%, p < 0.00001). However, no significant difference was detected in the rate of organ/space surgical site infection (4.0% vs 4.8%; p = 0.56) after elective colorectal surgery. LIMITATIONS: The meta-analysis was limited by the risk of bias because a majority of the studies did not use the blinding method. CONCLUSIONS: Oral systemic antibiotics and mechanical bowel preparation significantly lowered the incidence of surgical site infection after elective colorectal surgery compared with systemic antibiotics alone and mechanical bowel preparation.

3.
Kidney Int Rep ; 9(6): 1774-1782, 2024 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-38899221

RESUMO

Introduction: Longitudinal changes in residual kidney function have not been well-examined in patients starting chronic hemodialysis (HD). Methods: We analyzed urine volume and kidney solute clearances from timed urine collections and corresponding plasma samples from 42 patients randomized to incremental HD (n = 21) and conventional HD (n = 21) in the TwoPlus pilot study. Samples were collected before HD initiation (baseline); and at 6, 12, 24, and 48 weeks. We assessed temporal trends in urine volume, kidney urea and creatinine clearance, and correlations between urine volume and kidney solute clearance. Results: Residual kidney function parameters in all patients declined over time; the pattern of decline differed between urine volume and kidney solute clearances. Urine volume declined at a steady rate with median (quartile 1, quartile 3) percentage change relative to baseline of -10% (-36 to 29) at week 6 and -47% (-76 to 5) by week 48. Kidney urea and creatinine clearances exhibited a larger decline than urine volume at week 6, -32% (-61 to 8) and -47% (-57 to -20), respectively. The rate of decline subsequently slowed, reaching about 61% decline for both solutes by week 48. Conventional HD demonstrated larger declines in urine volume and kidney urea clearance than incremental HD at week 6. Urine volume showed moderate correlation with urea (R = 0.47) and weaker correlation with creatinine (R = 0.34). Conclusion: Despite gradual decrement in urine volume and kidney solute clearances, residual kidney function persists nearly 1 year after HD initiation. This knowledge could motivate increased practice of individualizing HD prescriptions by incorporating residual kidney function.

4.
J Nanosci Nanotechnol ; 13(3): 1858-62, 2013 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-23755606

RESUMO

SnO2 nanocrystals with coral-like porous structure were prepared with sol-gel method combining with an annealing process. The crystallite size and structure of SnO2 were characterized with X-ray diffraction and scanning electron microscope. The average coral-like nanostructrured SnO2 crystallite size is about 13 nm. The acetone gas sensors were obtained by sintering the mixture of coral-like porous nanostructured SnO2 and PdCl2, Ce(NO3)3 respectively. The acetone gas sensing properties of the mixed solid powder were measured by the commercial gas-sensing measurement system. Pd-doped sensors show decreased sensitivity to acetone, compared to that of the undoped sensor. On the other hand, the sensor based on 0.5 mol% Ce-doped shows the highest response to acetone at 190 degrees C. It exhibits a good linear relationship with the acetone concentration (10 - 1000 ppm) in logarithmic forms, which can provide potential applications for acetone detecting.

5.
J Interv Med ; 5(1): 23-27, 2022 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-35586283

RESUMO

Objectives: To provide data on the safety and efficacy of renal arterial embolization (RAE) in patients with high-grade blunt renal injury. Materials and methods: Fifteen patients with high-grade blunt renal injury (AAST grades IV-V) admitted to our hospital from July 2014 to December 2019 were retrospectively reviewed in this study. Their clinical success rate and complications were investigated accordingly. Results: Fifteen patients with high-grade blunt renal injury, 13 men and 2 women with an average age of 41.6 years, including 11 hemodynamically unstable patients and 4 stable patients, were treated with RAE. Among these patients, 73.3% (11 of 15) had grade IV, and 26.7% (4 of 15) had grade V injuries, while 53.3% (8 of 15) patients had concomitant injuries. One patient received main RAE and 14 patients received selective RAE. The clinical success rate after the first embolization was 93.3% (14 of 15). RAE was repeated and was successfully performed in one patient with sustained hematuria. No significant difference in creatinine levels was found before and after embolization. During the follow-up period of 2-82 months, two patients required tube drainage due to urine leaks, one patient developed renal failure requiring renal replacement therapy, and one patient developed secondary hypertension. Conclusions: RAE can provide a high success rate of hemostasis for both hemodynamically stable and unstable patients with high-grade blunt renal injury, and only minor complications are observed with this procedure.

6.
J Colloid Interface Sci ; 627: 343-354, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-35863193

RESUMO

Photocatalytic reduction of CO2 to high-energy products is an effective way to utilize solar energy and mitigate the greenhouse effect. In this paper, a series of CQDs/Bi12O17Cl2/NiAl-LDH (C/BOC/LDH) photocatalysts were prepared via a one-pot hydrothermal method, demonstrated excellent photocatalytic CO2 reduction performance. In the case of only water without any photosensitizer and sacrificial agent, the CO production rate on C/0.3BOC/LDH reached 16.4 µmol·g-1h-1, which is 6.7 times higher than that of the original LDH. The construction of Z-scheme heterojunctions inhibited the recombination of electrons with holes. The unique up-conversion PL behavior of CQDs benefitted the absorption of energy in the NIR by the photocatalyst. This study provides meaningful assistance for the design and construction of a ternary photocatalytic system with Z-scheme heterojunction and carbon-based co-catalyst.

7.
J Colloid Interface Sci ; 618: 300-310, 2022 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-35344883

RESUMO

As a renewable green energy, hydrogen has received widespread attention due to its huge potential in solving energy shortages and environment pollution. In this paper, a one-step solvothermal method was applied to grow ultra-thin g-C3N4 (UCN) nanosheets and NiS nanoparticles on the surface of ZnIn2S4 (ZIS). A ternary NiS/ZnIn2S4/ultra-thin-g-C3N4 composite material with dual high-speed charge transfer channels was constructed for the advancement of the photocatalytic H2 generation. The optimal ternary catalyst 1.5wt.%NiS/ZnIn2S4/ultra-thin-g-C3N4 (NiS/ZIS/UCN) achieved a H2 evolution yield reached to 5.02 mmolg-1h-1, which was 5.23 times superior than that of pristine ZnIn2S4 (0.96 mmolg-1h-1) and even outperform than that of the best precious metal modified 3.0 wt%Pt/ZnIn2S4 (4.08 mmolg-1h-1). The AQY at 420 nm could be achieved as high as 30.5%. The increased photocatalytic performance of NiS/ZIS/UCN could be ascribed to the type-I heterojunctions between intimated ZIS and UCN. In addition, NiS co-catalyst with large quantity of H2 evolution sites, could result in efficient photo-induced charges separation and migration. Furthermore, the NiS/ZIS/UCN composite exhibited excellent H2 evolution stability and recyclability. This work would also offer a reference for the design and synthesis of ternary co-catalyst with heterojunction composite for green energy conversion.

8.
Chemosphere ; 287(Pt 2): 132241, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34826928

RESUMO

S-scheme heterostructure can facilitate the separation of carriers while maintain outstanding redox capacity. A series of ternary Bi7O9I3/g-C3N4/Bi3O4Cl photocatalytic system was triumphantly synthesized via oil bath method in this work and used in photocatalytic degradation of phenol. The optimal TOC removal rate reached up to 93.57% under illumination for 160 min, which was slightly lower than phenol photodegradation (about 100%, 100 min). Correspondingly, the apparent rate constants for the decay of phenol are determined to be 0.0211 min-1. The experiment of free radical capture indicated that ·OH and ·O2- were the major oxidizing substances to degrade phenol. The products of phenol photodegradation were identified by high performance liquid chromatography (HPLC) and a possible degradation pathway was proposed. The characterization analysis and density functional theory (DFT) calculations demonstrated that dual S-scheme charge migration was generated at the interface of Bi7O9I3, g-C3N4 and Bi3O4Cl, contributing to an efficient separation of light-excited carriers. In the field of environmental remediation, the discovery of this work could open up promising vistas for designing bismuth-based ternary heterostructures with application potentiality.


Assuntos
Antibacterianos , Bismuto , Iodetos , Óxidos , Fenóis , Fotólise
9.
Macromol Biosci ; 22(1): e2100281, 2022 01.
Artigo em Inglês | MEDLINE | ID: mdl-34708919

RESUMO

Failing to regenerate native tendon tissue in chronic massive rotator cuff tears (CMRCTs) results in high retear rates after surgery. Gelatin is a hydrolyzed form of collagen which is bioactive and biocompatible. This study intends to investigate the suitability of integrating gelatin to poly (l-lactic acid) (PLLA) fibrous membranes for promoting the healing of CMRCTs. PLLA/Gelatin electrospun membranes (PGEM) are fabricated using electrospinning technology. The fourier transform infrared, static contact angles are tested sequentially. Cytocompatibility is evaluated with rat tendon fibroblasts and human umbilical endothelial cells (HUEVCs) lines. CMRCTs rat models are established and assigned into three groups (the sham group, the repaired group, and the augmentation group) to perform histomorphological and biomechanical evaluations. Gelatin is successfully integrated into PLLA fibrous membranes by the electrospinning technique. In vitro studies indicate that PGEM shows a great cytocompatibility for rat tendon fibroblasts and HUEVCs. In vivo studies find that applications of PGEM significantly promote well-aligned collagen I fibers formation and enhance biomechanical properties of the repaired tendon in CMRCTs rat models. In summary, gelatin promotes tendon fibroblasts and HUEVCs adhesion, migration, and proliferation on the PLLA fibrous membranes, and PGEM may provide a great prospect for clinical application.


Assuntos
Lesões do Manguito Rotador , Animais , Células Endoteliais , Gelatina/farmacologia , Ratos , Regeneração , Lesões do Manguito Rotador/cirurgia , Cicatrização
10.
Biomed Mater ; 16(4): 042007, 2021 04 01.
Artigo em Inglês | MEDLINE | ID: mdl-33793421

RESUMO

For decades, collagen has been among the most widely used biomaterials with several biomedical applications. Recently, researchers have shown a keen interest in collagen obtained from marine sources because of its biocompatibility, biodegradability, ease of extractability, safety, low immunogenicity, and low production costs. A wide variety of marine collagen-based scaffolds have been developed for bone tissue engineering, and these scaffolds display excellent biological effects. This review aims to provide an overview of the biological effects of marine collagen in bone engineering, such as promoting osteogenesis and collagen synthesis, inhibiting inflammation, inducing the differentiation of cartilage, and improving bone mineral density. Marine collagen holds great promise as a biomaterial in bone tissue engineering.


Assuntos
Organismos Aquáticos/química , Substitutos Ósseos , Osso e Ossos , Colágeno , Alicerces Teciduais , Animais , Osso e Ossos/citologia , Osso e Ossos/metabolismo , Humanos , Engenharia Tecidual
11.
Biofabrication ; 14(1)2021 12 03.
Artigo em Inglês | MEDLINE | ID: mdl-34798619

RESUMO

Mesenchymal stem cell (MSC)-derived extracellular vesicles (EVs) are promising candidates for regenerative medicine; however, the lack of scalable methods for high quantity EV production limits their application. In addition, signature EV-derived proteins shared in 3D environments and 2D surfaces, remain mostly unknown. Herein, we present a platform combining MSC microfiber culture with ultracentrifugation purification for high EV yield. Within this platform, a high quantity MSC solution (∼3 × 108total cells) is encapsulated in a meter-long hollow hydrogel-microfiber via coaxial bioprinting technology. In this 3D core-shell microfiber environment, MSCs express higher levels of stemness markers (Oct4, Nanog, Sox2) than in 2D culture, and maintain their differentiation capacity. Moreover, this platform enriches particles by ∼1009-fold compared to conventional 2D culture, while preserving their pro-angiogenic properties. Liquid chromatography-mass spectrometry characterization results demonstrate that EVs derived from our platform and conventional 2D culturing have unique protein profiles with 3D-EVs having a greater variety of proteins (1023 vs 605), however, they also share certain proteins (536) and signature MSC-EV proteins (10). This platform, therefore, provides a new tool for EV production using microfibers in one culture dish, thereby reducing space, labor, time, and cost.


Assuntos
Bioimpressão , Vesículas Extracelulares , Células-Tronco Mesenquimais , Vesículas Extracelulares/metabolismo , Células-Tronco Mesenquimais/metabolismo , Medicina Regenerativa , Tecnologia
12.
Dalton Trans ; 50(32): 11249-11258, 2021 Aug 28.
Artigo em Inglês | MEDLINE | ID: mdl-34341816

RESUMO

Promoting the separation of photogenerated electron-hole pairs and enhancing the charge carrier transfer are critical in photocatalysis. In our work, a ball-flower-like NiS/Zn3In2S6/g-C3N4 photocatalyst fabricated by a hydrothermal method exhibited superior performance for photocatalytic water splitting. The optimized 2.0% NiS/Zn3In2S6/g-C3N4 rivaled noble metal based Pt/g-C3N4 and showed an apparent quantum efficiency (AQE) of 24.3% at 420 nm, with a H2 yield of 4.135 mmol g-1 h-1, which was 30.4 and 9.51 times that of pure g-C3N4 and binary Zn3In2S6/g-C3N4 composites, respectively. The experimental and characterization results suggested that the heterojunction formed between Zn3In2S6/g-C3N4 and the decorating NiS co-catalyst cooperatively suppressed the electron-hole recombination and facilitated the charge carrier transfer, thus resulting in significant improvement of the H2 evolution performance. Moreover, the increased specific surface area and the enhanced visible-light absorption also contributed to superior water splitting performance. The prepared ternary catalytic system with the heterojunction and non-noble metal co-catalyst has great potential as an alternative to noble metals for achieving cost-efficient water splitting systems.

13.
Front Neurol ; 12: 721118, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34531816

RESUMO

Objective: This study was conducted in order to investigate the association of matrix metalloproteinase (MMP)-9 levels with phenotypes of moyamoya disease (MMD). Methods: This study included plasma samples from 84 MMD patients. The clinical variables of these patients were reviewed from the medical record. The serum concentration of tight junction, adherens junction proteins, and MMPs (MMP-2 and MMP-9) was determined using the ELISA method. Patients with hemorrhagic-onset MMD were compared with those with ischemic-onset MMD. Results: Compared with pediatric patients, the expression of MMP-9 was significantly higher, while the MMP-2 and vascular endothelial-cadherin were lower in adult patients. In adult subgroup analysis, hemorrhagic MMD patients exhibited significantly higher serum concentrations of MMP-9 compared with ischemic MMD patients. The ROC curve identified that a baseline serum MMP-9 level >1,011 ng/ml may be associated with spontaneous hemorrhage in adult MMD patients with 70.37% sensitivity and 71.88% specificity [area under curve (AUC), 0.73; 95% CI 0.597-0.864; P = 0.003]. A late Suzuki stage (>4) (OR 4.565, 95% CI 1.028-20.280, P = 0.046) and serum concentrations of MMP-9 >1,011 ng/ml (OR 7.218, 95% CI 1.826-28.533, P = 0.005) are risk predictors of hemorrhages in MMD patients. Hemorrhagic-type MMD patients had higher serum levels of MMP-9 and BBB permeability compared with ischemic-type MMD patients. Adult MMD patients had higher serum levels of MMP-9 and BBB permeability compared with pediatric patients. Conclusions: MMP-9 might serve as a biomarker for hemorrhage prediction in MMD. Serum MMP-9 level >1,011 ng/ml is an independent risk factor of MMD hemorrhagic strokes.

14.
Colloids Surf B Biointerfaces ; 204: 111808, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-33971613

RESUMO

Biocompatible polymers and drug delivery vehicles have been driving development in bone regeneration. However, most bone scaffolds show poor degradation and proliferation. In this study, a composite microsphere scaffold was prepared using vancomycin hydrochloride(VH)-loaded polytrimethylene carbonate(PTMC) microsphere (PTMC-VH). Adopting a thermal technique, a three-dimensional oleic acid-modified tricalcium phosphate (PTMC-OA-TCP)/PTMC-VH microsphere scaffold was prepared. It had a porosity of 41-47 % and pore size of 129-154 µm. The highest drug loading and release efficiency were obtained with the scaffold produced using 2.4 % polymer concentration and 0.5 %polyvinyl alcohol. The scaffold with PTMC-VH microsphereshad enhancedmechanical properties, water absorption capacity, and degradation. In addition, the PTMC-OA-TCP scaffold had comparable performance with bone cement control in terms of bone regeneration in vivo. In summary, the prepared bioactive scaffolds, which had favorable mechanical properties and facilitated osteogenesis, could be a promising alternative for bone cement in bone tissue engineering.


Assuntos
Fosfatos de Cálcio , Engenharia Tecidual , Materiais Biocompatíveis , Dioxanos , Microesferas , Polímeros , Porosidade , Alicerces Teciduais
15.
ChemSusChem ; 14(2): 539-557, 2021 Jan 21.
Artigo em Inglês | MEDLINE | ID: mdl-33216454

RESUMO

Photocatalytic hydrogen evolution can effectively alleviate the troublesome global energy crisis by converting solar energy into the chemical energy of hydrogen. In order to realize efficient hydrogen generation, a variety of semiconductor materials have been extensively investigated, including TiO2 , CdS, g-C3 N4 , metal-organic frameworks (MOFs), and others. In recent years, to achieve higher photocatalytic performance and reach the level of large-scale industrial applications, photocatalysts decorated with transition metal phosphides (TMPs) have shone brightly because of their low cost, stable physical and chemical properties, and substitution for precious metals of TMPs. This Review highlights the preparation methods and properties associated with photocatalysis of TMPs. Moreover, the H2 generation efficiency of photocatalysts loaded with TMPs and the roles of TMPs in catalytic systems are also studied systematically. Apart from being co-catalysts, several TMPs can also serve as host catalysts to boost the activity of photocatalytic composites. Finally, the development prospects and challenges of TMPs are put forward, which is valuable for future researchers to expand the application of TMPs in photocatalytic directions and to develop more active photocatalytic systems.

16.
ACS Appl Mater Interfaces ; 12(35): 39145-39153, 2020 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-32805894

RESUMO

In order to improve the thermoelectric properties of single-walled carbon nanotubes (SWCNTs), bilayer-like structures of graphene quantum dots (GQDs) and SWCNTs films (b-GQDs/SWCNTs) were prepared by directly coating GQDs on the surface of SWCNTs films. Compared to pristine SWCNT films (p-SWCNTs), the electrical conductivity of b-GQDs/SWCNTs increased while their Seebeck coefficient decreased. The special interface structure of GQDs and SWCNTs can not only improve carrier transport to increase electrical conductivity but also scatter phonons to reduce thermal conductivity. A maximum power factor (PF) of 51.2 µW·m-1·K-2 is obtained at 298 K for the b-GQDs/SWCNTs (2:100), which is higher than the PF of 40.9 µW·m-1·K-2 by p-SWCNTs. Incorporation of GQDs shows an obvious improvement in power factor and a significant reduction in the thermal conductivity for SWCNTs, and thus, preparation of b-GQDs/SWCNTs provides a new strategy to enhance the thermoelectric properties of SWCNTs-based materials.

17.
Gastroenterol Res Pract ; 2020: 9869742, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33488698

RESUMO

BACKGROUND: No consensus exists regarding which procedures should be performed to disinfect endoscopes and working platforms after COVID-19 patients have undergone endoscopy. METHODS: We analyzed the disinfection quality of endoscopes and working platforms after 11 COVID-19 patients had undergone endoscopy. CONCLUSIONS: For endoscopic preprocessing at the bedside, a key disinfection step is using a multienzyme stock solution. The nucleic acid tests for endoscopists, washers, endoscopes, and working platforms were all negative. Based on our experience with the 11 COVID-19 patients who had undergone endoscopy, we provide an endoscopic reprocessing method for the bedside endoscopic diagnosis and treatment of COVID-19 patients for reference.

18.
J Exp Clin Cancer Res ; 38(1): 148, 2019 Apr 05.
Artigo em Inglês | MEDLINE | ID: mdl-30953548

RESUMO

BACKGROUND: Recent Clinical trials of administration of epidermal growth factor receptor tyrosine kinase inhibitors (EGFR-TKIs) in combination with standard first-line chemotherapy have failed to improve survival in patients with advanced NSCLC, However, the sequential treatment with EGFR-TKIs and chemotherapy is expected to improve survival of NSCLC. The aim of this study is to test the antiproliferative effect of pemetrexed combined with icotinib in different sequences on non-small cell lung cancer (NSCLC) cell lines to determine the optimal combination schedule, and subsequently elaborated the potential mechanisms. METHODS: Six human lung cancer cell lines with wild-type or mutant EGFR gene were exposed to pemetrexed and icotinib combined in different sequences. Cell proliferation was examined by cell counting kit-8 (CCK-8) and colony formation assay; cell cycle and apoptosis were evaluated by flow cytometry; cell migration and invasion were measured by wound healing and transwell invasion assays respectively; protein expression was by detected by Western blot. RESULTS: The growth inhibition effect of pemetrexed combined with icotinib on NSCLC cells were schedule-dependent in vitro and in vivo. Treatment with pemetrexed followed by icotinib (P-I) had significantly stronger anticancer ability than treatment with icotinib followed by pemetrexed (I-P) and concomitant treatment with pemetrexed and icotinib (P + I). Cell cycle analysis revealed that pemetrexed blocked cells in S phase, whereas icotinib arrested cells in G1 phase. We also found that icotinib markedly enhanced the pro-apoptotic activity of pemetrexed via cytochrome-C/Caspase/Bcl-2 signaling pathway. In addition, our results showed that pemetrexed alone increased the levels of p-EGFR, p-AKT and p-MAPK, which were inhibited by icotinib. Finally, we showed that the washout period of icotinib was no less than 96 h. CONCLUSIONS: Sequential treatment of NSCLC cells with pemetrexed followed by icotinib had powerful antiproliferative effect, and it could become a novel effective combination therapy for NSCLC patients.


Assuntos
Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Éteres de Coroa/uso terapêutico , Neoplasias Pulmonares/tratamento farmacológico , Pemetrexede/uso terapêutico , Quinazolinas/uso terapêutico , Animais , Protocolos de Quimioterapia Combinada Antineoplásica/farmacologia , Linhagem Celular Tumoral , Éteres de Coroa/farmacologia , Sinergismo Farmacológico , Humanos , Neoplasias Pulmonares/genética , Camundongos , Camundongos Nus , Pemetrexede/farmacologia , Quinazolinas/farmacologia
19.
Sensors (Basel) ; 8(11): 7085-7096, 2008 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-27873916

RESUMO

A carbon nanotube/La-doped TiO2 (La-TiO2) nanocomposite (CLTN) was prepared by a procedure similar to a complex/adsorption process. Scanning electron microscopy (SEM) images show that the La-TiO2 distributes on the carbon nanotube walls. The CLTN was mixed with paraffin to form a CLTN paste for the CLTN paste electrode (CLTNPE). The electrochemical characteristics of CLTNPE were compared with that of conventional carbon electrodes such as the carbon paste electrode (CPE) and glass carbon electrode (GC). The CLTNPE exhibits electrochemical activity and was used to investigate the electrochemistry of nicotinic acid (NA). The modified electrode has a strong electrocatalytic effect on the redox of NA. The cyclic voltammetry (CV) redox potential of NA at the CLTNPE is 320 mV. The oxidation process of NA on the CLTNPE is pH dependent. A sensitive chronoamperometric response for NA was obtained covering a linear range from 1.0×10-6 mol·L-1 to 1.2×10-4 mol·L-1, with a detection limit of 2.7×10-7 mol·L-1. The NA sensor displays a remarkable sensitivity and stability. The mean recovery of NA in the human urine is 101.8%, with a mean variation coefficient (RSD) of 2.6%.

20.
Acta Crystallogr C ; 64(Pt 6): o322-5, 2008 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-18535340

RESUMO

Both the 1:1 and 2:1 molecular adducts of 4-methylimidazole (4-MeIm) and terephthalic acid (H2TPA) are organic salts, viz. C4H7N2+.C8H5O4-, (I), and 2C4H7N2+.C8H4O4(2-), (II), respectively. The component ions in (I) are linked by N-H...O and O-H...O hydrogen bonds into continuous two-dimensional layers built from R6(4)(32) hydrogen-bond motifs running parallel to the (100) plane. These adjacent two-dimensional layers are in turn linked by a combination of C-H...O, C-H...pi and pi-pi interactions into a three-dimensional network. In the crystal structure of (II), with the anion located on an inversion centre, only N-H...O hydrogen bonds result in two-dimensional layers built from R8(8)(42) hydrogen-bond motifs running parallel to the (102) plane. Being similar to those in (I), these layers are also linked by means of C-H...O, C-H...pi and pi-pi interactions, forming a three-dimensional network. This study indicates that, on occasion, a change of the reactant concentration can exert a pivotal influence on the construction of supramolecular structures based on hydrogen bonds.


Assuntos
Ácidos Ftálicos/química , Cristalografia por Raios X , Ligação de Hidrogênio , Imidazóis/química , Conformação Molecular
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