1.
Chembiochem
; 14(8): 950-3, 2013 May 27.
Artigo
em Inglês
| MEDLINE
| ID: mdl-23650044
RESUMO
Keep 'em methylated: The in situ preparation of the cofactor AdoMet was achieved by allowing the biosynthetic enzyme SalL to operate in the reverse direction by presentation of 5'-chloro-5'-deoxyadenosine at low salt concentrations. This reaction was readily coupled with DNA and small molecule methyltransferases to afford a regioselective method for chemo-enzymatic methylation and isotope incorporation.