Description and Validation of Flow-Through Chambers of Respirometry for Measuring Gas Exchange in Animal Trials.

Indirect calorimetry (IC) is a widely used method to study animal energy metabolism by measuring gas exchange. The accuracy of IC depends on detecting variations in signals reflecting the metabolic response, which can be challenging due to measurement noise and external factors. This study proposes a methodology to validate IC systems, including an easy-to-use spreadsheet for data computing, to verify accuracy and detect whole-system leaks. We conducted a recovery test using a simulation of CO2 dynamics in MS Excel and injecting a known CO2 concentration into four respirometry chambers. The thought flow rate of CO2 was observed and compared to the expected rate from the simulation. Data filtering and computing, including a detailed calculation of signals calibration, Bartholomew transformation, and noise reduction, was developed to obtain the gas exchange and heat production parameters using an open-circuit IC system. The results from the recovery test in our system show that the proposed methodology is accurate and precise. The proposed methodology and recovery test can be used to standardize the validation of IC systems together with adequate data computing, providing accurate measurements of animal energy metabolism in different environmental conditions and energy utilization from feeds.

Effect of feed restriction on the maintenance energy requirement of broiler breeders.

OBJECTIVE: This study aimed to evaluate the effect of the ad libitum and restricted feeding regimen on fasting heat production (FHP) and body composition. METHODS: Twelve Hubbard broilers breeders were selected with the same body weight and submitted in two feeding regimes: Restricted (T1) with feed intake of 150 g/bird/d and ad libitum (T2). The birds were randomly distributed on the treatments in two runs with three replications per treatment (per run). The birds were adapted to the feed regimens for ten days. After that, they were allocated in the open-circuit chambers and kept for three days for adaptation. On the last day, oxygen consumption (VO2) and carbon dioxide production (VCO2) were measured by 30 h under fasting. The respiratory quotient (RQ) was calculated as the VCO2/VO2 ratio, and the heat production (HP) was obtained using the Brower equation (1985). The FHP was estimated throughout the plateau of HP 12 hours after the feed deprivation. The body composition was analyzed by dual-energy X-ray absorptiometry scanning at the end of each period. Data were analyzed for one-way analysis of variance using the Minitab software. RESULTS: The daily feed intake was 30 g higher to T2 (p<0.01) than the T1. Also, the birds of the T2 had significatively (p<0.05) more oxygen consumption (+3.1 L/kg0.75/d) and CO2 production (+2.2 L/kg0.75/d). That resulted in a higher FHP 359±14 kJ/kg0.75/d for T2 than T1 296±17.23 kJ/kg0.75/d. In contrast, the RQ was not different between treatments, with an average of 0.77 for the fasting condition. In addition, protein and fat composition were not affected by the treatment, while a tendency (p<0.1) was shown to higher bone mineral content on the T1. CONCLUSION: The birds under ad libitum feeding had a higher maintenance energy requirement but their body composition was not affected compared to restricted feeding.

Response of Laying Hens to Repletion and Depletion in Dietary Balanced Protein.

This study was carried out to investigate the response of laying hens given a repletion or depletion in dietary balanced protein (BP) during the laying phase period. At the beginning of the rearing period (eight w-old), four-hundred pullets were equally distributed and received one of two experimental feeds: 1-Low BP (L) and 2-High BP (H). For the laying period (19 to 102 w-old), four feeding programs were designed based on the same treatments for rearing phases (LL, HH, LH, HL), where subsequent letters indicate the feed received during the rearing and laying period, respectively. The performance responses, egg quality, and body composition were periodically collected during the laying period. Two-way ANOVA repeated measures analysis was applied to evaluate the data. Nonlinear regression models with groups were used to compare treatments in the laying phase, with the treatments being the group evaluated. All performance traits were somehow influenced by the level of BP in the feed (p < 0.050). Hens subjected to the repletion treatment (LH) demonstrated a recovery in performance after 38 w-old. The opposite result was observed for hens on the depletion treatment (HL). All egg components were affected by dietary BP (p < 0.050). Laying hens demonstrated a limited capacity to overcome a reduction in dietary BP during production, but they were able to recover from a previous deficient feed once they were given an opportunity to do so.