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1.
Cancer Res ; 35(11 Pt 1): 3100-5, 1975 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-171063

RESUMO

A continuous human breast tumor cell line (BOT-2) was derived from an infiltrating duct carcinoma. The tumor cell line was grown as a monolayer in flasks, but the cells could be readily adapted to growth in roller cultures. These studies indicate that BOT-2 cells have a 16- to 18-hr doubling time and a modal chromosomal number of 63. The original BOT-2 cell culture has been in continuous cultivation for almost 2 years and has been passed 137 times. The BOT-2 cell line has been differentiated from HeLa cells by isoenzyme studies and chromosomal analysis.


Assuntos
Neoplasias da Mama/patologia , Carcinoma Intraductal não Infiltrante/patologia , Linhagem Celular , Fosfatase Alcalina/análise , Neoplasias da Mama/enzimologia , Carcinoma Intraductal não Infiltrante/enzimologia , Cromossomos , Técnicas de Cultura , Feminino , Glucosefosfato Desidrogenase/análise , Humanos , Isoenzimas/análise , Cariotipagem , Fatores de Tempo
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