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Lipid droplets (LDs) have increasingly been recognized as an essential organelle for eukaryotes. Although the biochemistry of lipid synthesis and degradation is well characterized, the regulation of LD dynamics, including its formation, maintenance, and secretion, is poorly understood. Here, we report that mice lacking Occludin (Ocln) show defective lipid metabolism. We show that LDs were larger than normal along its biogenesis and secretion pathway in Ocln null mammary cells. This defect in LD size control did not result from abnormal lipid synthesis or degradation; rather, it was because of secretion failure during the lactation stage. We found that OCLN was located on the LD membrane and was bound to essential regulators of lipid secretion, including BTN1a1 and XOR, in a C-terminus-dependent manner. Finally, OCLN was a phosphorylation target of Src kinase, whose loss causes lactation failure. Together, we demonstrate that Ocln is a downstream target of Src kinase and promotes LD secretion by binding to BTN1a1 and XOR.
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Gotículas Lipídicas/fisiologia , Metabolismo dos Lipídeos , Glândulas Mamárias Animais/metabolismo , Ocludina/metabolismo , Animais , Butirofilinas/metabolismo , Feminino , Lactação/metabolismo , Camundongos , Leite/metabolismo , Ocludina/genética , Quinases da Família src/antagonistas & inibidores , Quinases da Família src/metabolismoRESUMO
BACKGROUND: Previous studies have shown that peptides encoded by noncoding RNAs (ncRNAs) can be used as peptide drugs to alleviate diseases. We found that microRNA-31 (miR-31) is involved in the regulation of hypertension and that the peptide miPEP31, which is encoded by the primary transcript of miR-31 (pri-miR-31), can inhibit miR-31 expression. However, the role and mechanism of miPEP31 in hypertension have not been elucidated. METHODS: miPEP31 expression was determined by western blot analysis. miPEP31-deficient mice (miPEP31-/-) were used, and synthetic miPEP31 was injected into Ang II-induced hypertensive mice. Blood pressure was monitored through the tail-cuff method. Histological staining was used to evaluate renal damage. Regulatory T (Treg) cells were assessed by flow cytometry. Differentially expressed genes were analysed through RNA sequencing. The transcription factors were predicted by JASPAR. Luciferase reporter and electrophoretic mobility shift assays (EMSAs) were used to determine the effect of pri-miR-31 on the promoter activity of miPEP31. Images were taken to track the entry of miPEP31 into the cell. RESULTS: miPEP31 is endogenously expressed in target organs and cells related to hypertension. miPEP31 deficiency exacerbated but exogenous miPEP31 administration mitigated the Ang II-induced systolic blood pressure (SBP) elevation, renal impairment and Treg cell decreases in the kidney. Moreover, miPEP31 deletion increased the expression of genes related to Ang II-induced renal fibrosis. miPEP31 inhibited the transcription of miR-31 and promoted Treg differentiation by occupying the Cebpα binding site. The minimal functional domain of miPEP31 was identified and shown to regulate miR-31. CONCLUSION: miPEP31 was identified as a potential therapeutic peptide for treating hypertension by promoting Treg cell differentiation in vivo. Mechanistically, we found that miPEP31 acted as a transcriptional repressor to specifically inhibit miR-31 transcription by competitively occupying the Cebpα binding site in the pri-miR-31 promoter. Our study highlights the significant therapeutic effect of miPEP31 on hypertension and provides novel insight into the role and mechanism of miPEPs.
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Angiotensina II , Pressão Sanguínea , Modelos Animais de Doenças , Hipertensão , Rim , Camundongos Endogâmicos C57BL , Camundongos Knockout , MicroRNAs , Regiões Promotoras Genéticas , Linfócitos T Reguladores , Animais , MicroRNAs/metabolismo , MicroRNAs/genética , Hipertensão/induzido quimicamente , Hipertensão/metabolismo , Hipertensão/fisiopatologia , Hipertensão/genética , Sítios de Ligação , Pressão Sanguínea/efeitos dos fármacos , Linfócitos T Reguladores/efeitos dos fármacos , Linfócitos T Reguladores/metabolismo , Linfócitos T Reguladores/imunologia , Rim/metabolismo , Rim/patologia , Masculino , Camundongos , Regulação da Expressão Gênica , Transdução de Sinais , Proteínas Estimuladoras de Ligação a CCAAT/metabolismo , Proteínas Estimuladoras de Ligação a CCAAT/genética , Anti-Hipertensivos/farmacologia , HumanosRESUMO
The design of photonic crystals using novel materials is of great significance for the construction of high-performance, next-generation photonic crystal devices. We propose a universal Band structure-Transmission optimization-Band structure method based on moving asymptotic (MMA) method, which can be widely applied to photonic crystal structures. In this paper, we use the method to optimize the band structure of high temperature superconducting photonic crystal, and obtain a wider photonic bandgap and better band flatness in a specific frequency band. This method avoids the disadvantages of traditional scanning methods such as low efficiency and high resource consumption, allows multi-parameter optimization, and improves the accuracy and effectiveness of band modulation based on the iterative process of numerical calculation. The study provides some insights for the design of novel wide-bandgap optical devices.
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To prevent further decomposition of organic-inorganic hybrid perovskite by defects, in this work density functional theory was applied to explore the electronic properties, carrier surface mobility and theoretical photoelectric conversion efficiency (PCE) of passivating molecules with different fluorine atom content at the symmetric site of the benzene ring at different termination ends of MAPbI3, which shed light on the control of perovskite surface passivation by different element atoms in the same molecule. We found that the same molecule acts as a different passivation agent at different termination faces. Passivating molecules on the surface termination end by MAI play a Lewis acid role, with molecules with stronger dipole moments narrowing the band gap from the original 1.77 to 1.73 eV. The exciton binding energy of molecules with stronger dipole moments (0.187-0.292 meV) is significantly lower than that of MAPbI3 (0.332 meV), so the effective separation of interface electrons and holes can be realized. Bromopenta-fluorobenzene has a lower adsorption energy of -0.17 eV, which can stably adsorb on the surface of perovskite and increase visible light absorption. Ultimately, the theoretical PCE increased from 15.8% to 16.16%. In addition, on the surface terminated by PbI2, BrB with a strong dipole moment can provide electrons for Pb2+ and act as a Lewis base. At the surface end, it can form an ionic bond with Pb2+, while the antibonding molecular orbital characteristic is dominant, which increases the band gap from 1.76 to 1.87 eV. After increasing to 4-F-BrB, the fluorine atom has strong electronegativity and can easily bond with Pb2+. The conjugate π cycle intensifies the promotion of electron transfer, reducing the work function from 5.262 to 4.703 eV, reducing the effective electron and hole mass (0.514, 0.204 m0), and improving the photovoltaic performance. Finally, increasing the number of passivation molecules resulted in a decrease in the PCE from 15.93% to 14.75%.
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Tissue architecture determines its unique physiology and function. How these properties are intertwined has remained unclear. Here we show that the metabolic enzyme CTP synthase (CTPS) form filamentous structures termed cytoophidia along the adipocyte cortex in Drosophila adipose tissue. Loss of cytoophidia, whether due to reduced CTPS expression or a point mutation that specifically abrogates its polymerization ability, causes impaired adipocyte adhesion and defective adipose tissue architecture. Moreover, CTPS influences integrin distribution and dot-like deposition of type IV collagen (Col IV). Col IV-integrin signaling reciprocally regulates the assembly of cytoophidia in adipocytes. Our results demonstrate that a positive feedback signaling loop containing both cytoophidia and integrin adhesion complex couple tissue architecture and metabolism in Drosophila adipose tissue.
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Carbono-Nitrogênio Ligases , Colágeno Tipo IV , Animais , Tecido Adiposo/metabolismo , Carbono-Nitrogênio Ligases/química , Carbono-Nitrogênio Ligases/genética , Carbono-Nitrogênio Ligases/metabolismo , Drosophila/metabolismo , IntegrinasRESUMO
Tight junctions (TJs) are fundamental features of both epithelium and endothelium and are indispensable for vertebrate organ formation and homeostasis. However, mice lacking Occludin (Ocln) develop relatively normally to term. Here we show that Ocln is essential for mammary gland physiology, as mutant mice fail to produce milk. Surprisingly, Ocln null mammary glands showed intact TJ function and normal epithelial morphogenesis, cell differentiation, and tissue polarity, suggesting that Ocln is not required for these processes. Using single-cell transcriptomics, we identified milk-producing cells (MPCs) and found they were progressively more prone to endoplasmic reticulum (ER) stress as protein production increased exponentially during late pregnancy and lactation. Importantly, Ocln loss in MPCs resulted in greatly heightened ER stress; this in turn led to increased apoptosis and acute shutdown of protein expression, ultimately leading to lactation failure in the mutant mice. We show that the increased ER stress was caused by a secretory failure of milk proteins in Ocln null cells. Consistent with an essential role in protein secretion, Occludin was seen to reside on secretory vesicles and to be bound to SNARE proteins. Taken together, our results demonstrate that Ocln protects MPCs from ER stress by facilitating SNARE-dependent protein secretion and raise the possibility that other TJ components may participate in functions similar to Ocln.
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Estresse do Retículo Endoplasmático/efeitos dos fármacos , Exocitose/fisiologia , Ocludina/farmacologia , Substâncias Protetoras/farmacologia , Proteínas SNARE/metabolismo , Animais , Apoptose , Diferenciação Celular , Epitélio , Feminino , Homeostase , Lactação , Glândulas Mamárias Animais/metabolismo , Glândulas Mamárias Animais/patologia , Camundongos , Camundongos Knockout , Leite/metabolismo , Morfogênese , Ocludina/genética , Gravidez , Junções Íntimas/metabolismo , TranscriptomaRESUMO
A simple and sensitive liquid chromatography tandem mass spectrometry method was established and validated for the quantitative determination of abrine, hypaphorine, schaftoside and soyasaponin Bb in rat plasma. After preparation by protein precipitation with acetonitrile, the analytes and internal standard were separated on a Waters CORTECS T3 column using acetonitrile containing 0.1% formic acid and 0.1% formic acid in water as mobile phase by gradient elution in 2 min. The method showed excellent linearity over the range of 5-500 ng/ml with acceptable intra- and inter-day precision, accuracy, matrix effect and recovery. The stability assay indicated that the four analytes were stable during the analysis process. The method was applied to a pharmacokinetic study of Abrus cantoniensis Hance in rats. The result suggested that after oral administration, the four analytes were quickly absorbed into the plasma. The dose-normalized exposure of hypaphorine was the highest with a long elimination half-life (t1/2 9.83 h), followed by abrine and schaftoside with t1/2 values of 1.07 and 1.15 h. The dose normalized exposure of soyasaponin Bb was the lowest, which is possibily due to the high polarity and poor permeability. This study provides a basis for elucidating the material foundation of A. cantoniensis Hance.
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Abrus , Espectrometria de Massas em Tandem , Ratos , Animais , Cromatografia Líquida/métodos , Espectrometria de Massas em Tandem/métodos , Cromatografia Líquida de Alta Pressão/métodos , Alcaloides Indólicos , Administração Oral , Reprodutibilidade dos TestesRESUMO
This study was designed to investigate the correlation of microsatellite status (MS) and Epstein-Barr virus (EBV) infection with the clinical characteristics of gastric cancer (GC) patients. MS was detected by immunohistochemistry. EBV was detected by in situ hybridization. There were 31.3% cases showed mismatch repair-deficient (dMMR)/ microsatellite instability (MSI) and 68.7% cases showed mismatch repair-proficient (pMMR)/ microsatellite stability (MSS). The dMMR/MSI was more common in the elderly, in patients with cardia GC, smaller tumor diameter or non-poorly differentiated carcinoma. The survival in dMMR/MSI patients tended to be longer than that in pMMR/MSS patients. Total 7.6% cases showed EBV-positive (EBV(+)) among 198 GC patients. EBV(+) was more common in patients with advanced GC or poorly differentiated adenocarcinoma. MSI was more common in EBV-negative (EBV(-)) patients than in EBV(+) patients. The dMMR/MSI patients with stage II GC benefited from chemotherapy. The survival of EBV(+) patients tended to be longer than that of EBV(-) patients.
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Infecções por Vírus Epstein-Barr , Neoplasias Gástricas , Idoso , Humanos , Neoplasias Gástricas/genética , Infecções por Vírus Epstein-Barr/complicações , Infecções por Vírus Epstein-Barr/genética , Herpesvirus Humano 4/genética , Repetições de MicrossatélitesRESUMO
Semiconductor photocatalytic technology has shown great prospects in converting solar energy into chemical energy to mitigate energy crisis and solve environmental pollution problems. The key issue is the development of high-efficiency photocatalysts. Various strategies in the state-of-the-art advancements, such as heterostructure construction, heteroatom doping, metal/single atom loading, and defect engineering, have been presented for the graphitic carbon nitride (g-C3N4)-based nanocomposite catalysts to design their surface chemical environments and internal electronic structures to make them more suitable for different photocatalytic applications. In this review, nanoarchitecture design, synthesis methods, photochemical properties, potential photocatalytic applications, and related reaction mechanisms of the modified high-efficiency carbon nitride-based photocatalysts were briefly summarized. The superior photocatalytic performance was identified to be associated with the enhanced visible-light response, fast photoinduced electron-hole separation, efficient charge migration, and increased unsaturated active sites. Moreover, the further advance of the visible-light harvesting and solar-to-energy conversions are proposed.
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BACKGROUND: Insect olfactory proteins can transmit chemical signals in the environment that serve as the basis for foraging, mate searching, predator avoidance and oviposition selection. Semanotus bifasciatus is an important destructive borer pest, but its olfactory mechanism is not clear. We identified the chemosensory genes of S. bifasciatus in China, then we conducted a phylogenetic analysis of the olfactory genes of S. bifasciatus and other species. And the expression profiles of odorant binding proteins (OBPs) genes in different tissues and different genders of S. bifasciatus were determined by quantitative real-time PCR for the first time. RESULTS: A total of 32 OBPs, 8 chemosensory proteins (CSPs), 71 odorant receptors (ORs), 34 gustatory receptors (GRs), 18 ionotropic receptors (IRs), and 3 sensory neuron membrane proteins (SNMPs) were identified. In the tissue expression analysis of OBP genes, 7 OBPs were higher expressed in antennae, among them, SbifOBP2, SbifOBP3, SbifOBP6, SbifOBP7 and SbifOBP20 were female-biased expression, while SbifOBP1 was male-biased expression and SbifOBP22 was no-biased expression in antennae. In addition, the expressed levels of SbifOBP4, SbifOBP12, SbifOBP15, SbifOBP27 and SbifOBP29 were very poor in the antennae, and SbifOBP4 and SbifOBP29 was abundant in the head or legs, and both of them were male-biased expression. While SbifOBP15 was highly expressed only at the end of the abdomen with its expression level in females three times than males. Other OBPs were expressed not only in antennae but also in various tissues. CONCLUSION: We identified 166 olfactory genes from S. bifasciatus, and classified these genes into groups and predicted their functions by phylogenetic analysis. The majority of OBPs were antenna-biased expressed, which are involved in odor recognition, sex pheromone detection, and/or host plant volatile detection. However, also some OBPs were detected biased expression in the head, legs or end of the abdomen, indicating that they may function in the different physiological processes in S. bifasciatus.
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Besouros , Receptores Odorantes , Animais , Antenas de Artrópodes/metabolismo , Besouros/genética , Besouros/metabolismo , Feminino , Perfilação da Expressão Gênica , Proteínas de Insetos/genética , Proteínas de Insetos/metabolismo , Masculino , Odorantes , Filogenia , Receptores Odorantes/genética , Receptores Odorantes/metabolismo , TranscriptomaRESUMO
A series of 4-(phenoxymethyl)-1H-1,2,3-triazole derivatives were designed, synthesized, and evaluated for their xanthine oxidase (XO) inhibitory activities. Among these compounds, 9m emerged as the most effective XO inhibitor with an IC50 value of 0.70 µM, which was approximately 14-fold more potent than allopurinol. Additionally, compound 9m displayed favorable drug-like properties with ligand efficiency (LE) and lipophilic ligand efficiency (LLE) values of 0.33 and 3.41, respectively. We further explored the binding mode of 9m in complex with XO by molecular docking and molecular dynamics studies. In vivo hypouricemic studies also suggested that 9m could effectively lower the serum uric acid levels of rat. In summary, compound 9m could be a promising lead for further development of XO inhibitors.
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Descoberta de Drogas , Inibidores Enzimáticos/farmacologia , Triazóis/farmacologia , Xantina Oxidase/antagonistas & inibidores , Animais , Relação Dose-Resposta a Droga , Inibidores Enzimáticos/síntese química , Inibidores Enzimáticos/química , Hiperuricemia/induzido quimicamente , Hiperuricemia/tratamento farmacológico , Hiperuricemia/metabolismo , Ligantes , Modelos Moleculares , Estrutura Molecular , Ácido Oxônico , Ratos , Relação Estrutura-Atividade , Triazóis/síntese química , Triazóis/química , Ácido Úrico/antagonistas & inibidores , Ácido Úrico/sangue , Xantina Oxidase/metabolismoRESUMO
Pairing different cations (R+) to stabilize cyclo-N5- is the main synthesis path for non-metallic cyclo-pentazolate (cyclo-N5-) salts. As novel energetic materials (EMs), crystalline packing-force of cyclo-N5- salts has been a puzzle, and whether cyclo-N5- is protonated also is a controversial issue. In this paper, four non-metallic cyclo-N5- salts, PHAC, N2H5N5, NH3OHN5, and NH4N5, are quantitatively studied by coupling first-principle method and bond-strength analyzing technology. Different from the traditional CHON-EMs (molecular crystal) and azide-EMs (ionic crystal), the four salts are stabilized by 3D hydrogen bond (HB) networks. One new type of hydrogen bond, protonated HB (p-H, R-Hâ¯N5-), is discovered to be a key stabilizing factor for cyclo-N5-. Proton competition mechanism between R and cyclo-N5- in p-H HB showed that cyclo-N5- cannot be protonated into HN5. In general, p-H HB can be adopted to estimate the stability of novel non-metallic cyclo-N5- EMs. Such findings have great significance for future design and performance prediction of novel cyclo-N5- EMs in both theoretical and experimental aspects.
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Photocatalytic water splitting, which can use abundant and clean solar energy, is friendly to the environment and consumes less energy. This work predicts the type-II ß-AsP/g-C6N6 van der Waals (vdW) heterostructure as a promising photocatalyst for water splitting using first-principles calculations. Compared with the g-C6N6 monolayer, the ß-AsP/g-C6N6 heterostructure not only can effectively separate photogenerated electron and hole pairs but also shows considerable light absorption and better photocatalyst performances. The optical absorption coefficient of the ß-AsP/g-C6N6 heterostructure has also extremely increased, more than ten times that of the g-C6N6 monolayer. The ß-AsP/g-C6N6 heterostructure with little compression (-2%) could be conducive to the hydrogen evolution reaction with a Gibbs free energy closer to zero (-0.46 eV) compared to monolayers. The oxygen evolution reaction has a low theoretical overpotential of 0.63 V. These results indicate that it exhibits favorable performances as a photocatalyst. This work predicts the possible application of the ß-AsP/g-C6N6 heterostructure as a photocatalyst and expands the scope of renewable energy devices.
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Na-, K- and Mg-ion batteries (NIBs, KIBs and MIBs) have drawn considerable interest due to their high abundance and excellent safety. However, the lack of high-performance anode materials is a major obstacle to its development. A metallic SnB planar monolayer is predicted by using the two-dimensional global minimum structure search method of swarm intelligence. Based on first-principles calculations, we proved that the metal SnB monolayer has high binding energy and excellent dynamical, thermal and mechanical stability. It is worth noting that the SnB monolayer has several stable adsorption sites for Na-, K- and Mg-ions, so it has a high theoretical capacity of 620.93, 517.44 and 620.93 mA h g-1, respectively. For Na-, K- and Mg-ion batteries, the low diffusion barriers of the SnB monolayer are 0.22, 0.07 and 0.68 eV, and the low average open circuit voltages are 0.42, 0.49 and 0.23 V, which ensure long service life and fast charging in practical applications. In addition, it is proved that the SnB monolayer maintains excellent conductivity and stability during the charge-discharge process. The results show that the SnB monolayer offers innovative advantages for the development of new two-dimensional planar structures that further advance the development of anode materials for metal ion batteries.
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In this study, switchable hydrophilic solvent-based dispersive liquid-liquid microextraction coupled with high-performance liquid chromatography was developed for the determination of four sulfonylurea herbicides in soils. For the first time, the sample pretreatment was achieved due to the similar acid-base status of sulfonylurea herbicides and switchable hydrophilic solvent. In the extraction step, sulfonylurea herbicides were extracted as anions and transferred to an alkaline solution with switchable hydrophilic solvent anions. In the concentration step, two types of anions were transformed to their molecular state after the aqueous solution was acidified. In addition, the dispersion and microextraction processes were completed efficiently with the simultaneous formation of analytes and extractants. The factors affecting the extraction performance were optimized. Under the optimized conditions, good linearity was observed for each herbicide with correlation coefficients ranging from 0.9952 to 0.9978. The limits of detection were in the range of 0.1-0.2 µg/g. Moreover, the relative recoveries of the sulfonylurea herbicides at spiking levels of 0.5, 1, and 1.5 µg/g in soil samples were between 75 and 111% (relative standard deviations: 0.4-11.4%). Therefore, the proposed method in this study could be successfully applied to the analysis of four types of sulfonylurea herbicides in soil samples.
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No RNA is completely naked from birth to death. RNAs function with and are regulated by a range of proteins that bind to them. Therefore, the development of innovative methods for studying RNA-protein interactions is very important. Here, we developed a new tool, the CRISPR-based RNA-United Interacting System (CRUIS), which captures RNA-protein interactions in living cells by combining the power of CRISPR and PUP-IT, a novel proximity targeting system. In CRUIS, dCas13a is used as a tracker to target specific RNAs, while proximity enzyme PafA is fused to dCas13a to label the surrounding RNA-binding proteins, which are then identified by mass spectrometry. To identify the efficiency of CRUIS, we employed NORAD (Noncoding RNA activated by DNA damage) as a target, and the results show that a similar interactome profile of NORAD can be obtained as by using CLIP (crosslinking and immunoprecipitation)-based methods. Importantly, several novel NORAD RNA-binding proteins were also identified by CRUIS. The use of CRUIS facilitates the study of RNA-protein interactions in their natural environment, and provides new insights into RNA biology.
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Proteínas Associadas a CRISPR , Proteínas de Ligação a RNA/metabolismo , Ribonucleases , Sistemas CRISPR-Cas , Células HEK293 , Humanos , Imunoprecipitação , Espectrometria de Massas , RNA/metabolismoRESUMO
Two-dimensional (2D) PtSe2 has emerged as a promising ultrathin electrocatalyst due to its excellent catalytic activity and conductivity. However, the PtSe2 basal plane is inert for the hydrogen evolution reaction (HER), which greatly limits its electrocatalytic performance. Here, in light of theoretical calculations, we designed a facile approach for activating the 2D PtSe2 basal plane for the HER by simultaneously introducing atomic vacancies of Se, Pt, and Pt clusters through a mild Ar plasma treatment. We tracked changes in the structures and catalytic performance of PtSe2 by combining microscopic imaging, spectroscopic mapping, and electrochemical measurements in microcells. The highest performance of the activated PtSe2 basal plane that we obtained was superior to those of other 2D transition metal dichalcogenide-based electrocatalysts measured in microcells in terms of the overpotential, the Tafel slope, and the exchange current density. This study demonstrates the great potential of activated 2D PtSe2 as an ultrathin catalyst for the HER and provides new insights on the rational design of 2D electrocatalysts.
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Sirex noctilio Fabricius (Hymenoptera Siricidae) is a major quarantine pest responsible for substantial economic losses in the pine industry. To achieve better pest control, (Z)-3-decen-ol was identified as the male pheromone and used as a field chemical trapping agent. However, the interactions between odorant-binding proteins (OBPs) and pheromones are poorly described. In this study, SnocOBP9 had a higher binding affinity with Z3D (Ki = 1.53 ± 0.09 µM) than other chemical ligands. Molecular dynamics simulation and binding mode analysis revealed that several nonpolar residues were the main drivers for hydrophobic interactions between SnocOBP9 and Z3D. Additionally, computational alanine scanning results indicated that five amino acids (MET54, PHE57, PHE71, PHE74, LEU116) in SnocOBP9 could potentially alter the binding affinity to Z3D. Finally, we used single-site-directed mutagenesis to substitute these five residues with alanine. These results imply that the five residues play crucial roles in the SnocOBP9-Z3D complex. Our research confirmed the function of SnocOBP9, uncovered the key residues involved in SnocOBP9-Z3D interactions, and provides an inspiration to improve the effects of pheromone agent traps.
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Himenópteros , Receptores Odorantes , Alanina/metabolismo , Animais , Proteínas de Insetos/genética , Proteínas de Insetos/metabolismo , Masculino , Feromônios/metabolismo , Ligação Proteica , Receptores Odorantes/metabolismoRESUMO
BACKGROUND: The woodwasp Sirex noctilio Fabricius is a major quarantine pest worldwide that was first discovered in China in 2013 and mainly harms Pinus sylvestris var. mongolica Litv.. S. nitobei Matsumura is a native species in China and is closely related to S. noctilio. Recently, the two woodwasps species were found attacking the P. sylvestris var. mongolica Litv in succession. The olfactory system is the foundation of insect behavior. Olfactory genes were identified through antennal transcriptome analysis. The expression profiles odorant binding proteins (OBPs) were analyzed with RT-qPCR. RESULTS: From our transcriptome analysis, 16 OBPs, 7 chemosensory proteins (CSPs), 41 odorant receptors (ORs), 8 gustatory receptors (GRs), 13 ionotropic receptors (IRs), and one sensory neuron membrane protein (SNMP) were identified in S. noctilio, while 15 OBPs, 6 CSPs, 43 ORs, 10 GRs, 16 IRs, and 1 SNMP were identified in S. nitobei. Most of the olfactory genes identified in two species were homologous. However, some species-specific olfactory genes were identified from the antennal transcriptomes, including SnocOBP13, SnocCSP6, SnocOR26, SnocGR2, SnocIR7 in S. noctilio and SnitGR9, SnitGR11, SnitIR17 in S. nitobei. In total, 14 OBPs were expressed primarily in the antennae. SnocOBP9 and SnitOBP9, identified as PBP homologues, were sex-biased expression in two siricid, but with different pattern. SnocOBP11 and SnitOBP11 were highly expressed in antennae and clearly expressed in external genitalia. SnocOBP7 and SnitOBP7 were highly expressed in male genitalia. SnocOBP3 and SnocOBP10 were highly expressed in female genitalia and male heads, while SnitOBP3 and SnitOBP10 did not show obvious tissue bias. CONCLUSION: We analyzed 86 and 91 olfactory genes from S. noctilio and S. nitobei, respectively. Most of the olfactory genes identified were homologous, but also some species-specific olfactory genes were identified, which indicated the similarities and differences of the molecular mechanisms between the two closely-related species. Different expression in the antennae, external genitals or heads, exhibiting an obvious sex bias, suggested their different role in recognizing sex pheromones or plant volatiles. Species-specific expression for several OBPs genes may suggest that they strengthened or lost their original function during species differentiation, resulting in olfactory differences between the two species.
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Himenópteros , Receptores Odorantes , Animais , Antenas de Artrópodes/metabolismo , Proteínas de Transporte , China , Feminino , Perfilação da Expressão Gênica , Himenópteros/genética , Himenópteros/metabolismo , Proteínas de Insetos/genética , Proteínas de Insetos/metabolismo , Masculino , Odorantes , Filogenia , Receptores Odorantes/genética , Receptores Odorantes/metabolismo , TranscriptomaRESUMO
Yttrium oxide (Y2O3) has garnered some attention in view of its potential to be integrated into a wide range of high-strength structural components, microelectronic and optoelectronic devices. However, the nonlinear optical research of this promising material has not been implemented yet. In this paper, not only the electronic band structures of Y2O3 are theoretically calculated but also the optical nonlinearity of Y2O3 is validated by using the fiber laser as a platform. Meanwhile, the influence of sample thickness on laser performance is further explored by using Y2O3 saturable absorbers with different thickness. Results indicate that Y2O3 not only has impressive optical nonlinearity but also is beneficial to the investigation of ultrafast photons by adjusting the thickness of Y2O3. Therefore, Y2O3 can be used as a potential saturable absorber candidate for in-depth research and application.