Flow rate influence on sediment depth estimation in sewers using temperature sensors.

Enhancing sediment accumulation monitoring techniques in sewers will enable a better understanding of the build-up processes to develop improved cleaning strategies. Thermal sensors provide a solution to sediment depth estimation by passively monitoring temperature fluctuations in the wastewater and sediment beds, which allows evaluation of the heat-transfer processes in sewer pipes. This study analyses the influence of the flow conditions on heat-transfer processes at the water-sediment interface during dry weather flow conditions. For this purpose, an experimental campaign was performed by establishing different flow, temperature patterns, and sediment depth conditions in an annular flume, which ensured steady flow and room-temperature conditions. Numerical simulations were also performed to assess the impact of flow conditions on the relationships between sediment depth and harmonic parameters derived from wastewater and sediment-bed temperature patterns. Results show that heat transfer between water and sediment occurred instantaneously for velocities greater than 0.1 m/s, and that sediment depth estimations using temperature-based systems were barely sensitive to velocities between 0.1 and 0.4 m/s. A depth estimation accuracy of ±7 mm was achieved. This confirms the ability of using temperature sensors to monitor sediment build-up in sewers under dry weather conditions, without the need for flow monitoring.

Influence of seasonal activities and traffic conditions on the accumulation and particle size distribution of gully pot sediments.

Gully pots (GPs) are ubiquitously used in urban catchments, to direct surface runoff into piped sewer systems, reduce the risk of sediment-induced pipe blockages and the loading of solids and associated contaminants on the receiving waters. Over time, the build-up of sediments in GPs will lead to impaired hydraulic performance, putting the urban catchment at risk of flooding. However, GP maintenance strategies lack both a robust evidence base and clear aims against which their effectiveness can be benchmarked. An improved understanding of the factors influencing long-term in-situ solids accumulation in GPs is required for further assessment and optimisation of the GP maintenance scheme. As a contribution to addressing these knowledge gaps, sediments from 27 GPs located in urban catchments associated with three street feature types (roundabout; crossing; straight road) and varied traffic intensities were collected on two occasions, representing two distinct seasonal accumulation periods. Parameters including GP fullness levels, particle size distribution (PSD), and solids mass accumulation rates were evaluated with regards to the impacts of seasonal activities, traffic conditions as well as configuration and dimension of GPs. The results indicate impacts of seasonal activities on solids accumulation rates were not consistent across all GPs, with the magnitude of the difference larger following winter-spring catchment practices. Additionally, synergistic impacts of seasonal activities and traffic conditions on PSD and solids accumulation rates were observed. For example, the winter road safety measures may enhance the impacts of the characteristic vehicle turning motions at roundabouts, contributing to the negative correlation identified between traffic intensities and solids accumulation rates. The overall exhibited large variations in sump fullness levels (4 %-73 %) and solids dry mass (0.5-51 kg) directly challenged the commonly adopted generalised GP maintenance frequency without taking the catchment-specific activities into consideration.

Evaluation of measured dissolved and bio-met predicted bioavailable Cu, Ni and Zn concentrations in runoff from three urban catchments.

Urban runoff is a diffuse source of pollution contributing to the poor ecological and chemical status of surface waters. Whilst the EU Priority Hazardous Substances Directive now identifies environmental quality standards for selected metals in relation to the bioavailable metal fraction the relationship between analytically determined metal size fractions transported by urban runoff and the often variably defined concept of bioavailability has not been thoroughly evaluated. This paper provides a review of the terminology used within urban runoff studies to characterise metal fractions and behaviour. Measured dissolved and truly dissolved (determined by ultrafiltration; <3000 molecular weight cutoff) Cu, Ni, and Zn concentrations are also compared to the bioavailable metal fraction (as predicted using Bio-met, a simplified biotic ligand model) in snowmelt and rainfall derived runoff samples from three urban catchments. The study shows that predicted bioavailable concentrations were significantly lower than truly dissolved concentrations for all metals and discusses current bioavailability modelling parameters in relation to rainfall and snowmelt runoff data sets. Statistical analysis of relationships between field and predicted bioavailable data sets indicate that the bioavailable fractions originate from both colloidal and truly dissolved fractions.

An assessment of gully pot sediment scour behaviour under current and potential future rainfall conditions.

Gully pots actively trap sediments transported by urban runoff to prevent in-pipe blockages and surface flooding. However, due to poor maintenance (resulting in sediment build-up) and increasingly extreme wet weather events, the scour of previously-deposited sediments from gully pots is identified as a potential contributor to EU Water Framework Directive failure. While basal sediment scour deterministic models have been developed and validated using laboratory and field gully pot data sets, the ability of these models to predict behaviour at sites other than those for which they were established has not been addressed. Nor has the impact of future rainfall predictions on the role of gully pots as sediment sources been systematically examined. As a contribution to addressing these knowledge gaps, the performance of two gully pot basal sediment scour models of distinct complexity levels are evaluated under current and future rainfall conditions. The output from Model One suggests that the scour-induced total suspended solids in gully pot discharge can be kept well below 25 mg/L if the gully pot fullness level is maintained at under 60%. Results identify the opportunity to incorporate the actual/targeted ecological status of recipients in scheduling gully pot maintenance operations and that proactive gully pots maintenance will reduce the impacts of increased rainfall intensity/duration on the magnitude of sediment scour. Results from Model Two suggest that fine sediments are particularly susceptible to in-pot scour. For example, sediment with a specific gravity of 1.1 and diameter of >63 µm accounts for 50% of scour-induced total suspended solids in gully pot discharge. The effluent suspended solids concentrations predicted by the two models differ by up to two orders of magnitude. However, without further empirical field data pertaining to their respective competences/applications, neither model could be discounted at this stage. For example, the use of Model One is more appropriate in the establishment of gully pot maintenance schedules, with Model Two more suited to the dimensioning of gully pots based on performance requirements. This application, however, relies on the development and adoption of a more stringent regulation on gully pots discharge.

Implementing sustainable drainage systems for urban surface water management within the regulatory framework in England and Wales.

The UK 2007 floods resulted in damages estimated to exceed over £4 billion. This triggered a national review of strategic flood risk management (Pitt, 2008) with its recommendations informing and implemented by the Flood and Water Management, Act (FWMA, 2010). Estimating that up to two-thirds of properties flooded in the 2007 event as a direct result of overloaded sewer systems, the FWMA set out an ambitious overhaul of flood risk management approaches including identifying bodies responsible for the management of local flood risk (local municipalities) and the development of over-arching Lead Local Flood Authorities (LLFAs) at a regional level. LLFAs duties include developing local flood risk management strategies and, aligned with this, many LLFAs and local municipalities produced sustainable drainage system (SUDS) guidance notes. In parallel, changes to the national planning policy framework (NPPF) in England give priority to the use of SUDS in new major developments, as does the related Town and Country Planning Order (2015). However, whilst all three pieces of legislation refer to the preferential use of SUDs, these requirements remain "economically proportionate" and thus the inclusion of SUDS within development controls remain desirable - but not mandatory - obligations. Within this dynamic policy context, reignited most recently by the December 2015 floods, this paper examines some of the challenges to the implementation of SUDS in England and Wales posed by the new regulatory frameworks. In particular, it examines how emerging organisational procedures and processes are likely to impact on future SUDS implementation, and highlights the need for further cross-sectoral working to ensure opportunities for cross-sectoral benefits- such as that accrued by reducing stormwater flows within combined sewer systems for water companies, property developers and environmental protection - are not lost.

The sources, impact and management of car park runoff pollution: a review.

Traffic emissions contribute significantly to the build-up of diffuse pollution loads on urban surfaces with their subsequent mobilisation and direct discharge posing problems for receiving water quality. This review focuses on the impact and mitigation of solids, metals, nutrients and organic pollutants in the runoff deriving from car parks. Variabilities in the discharged pollutant levels and in the potentials for pollutant mitigation complicate an impact assessment of car park runoff. The different available stormwater best management practices and proprietary devices are reported to be capable of reductions of between 20% and almost 100% for both suspended solids and a range of metals. This review contributes to prioritising the treatment options which can achieve the appropriate pollutant reductions whilst conforming to the site requirements of a typical car park. By applying different treatment scenarios to the runoff from a hypothetical car park, it is shown that optimal performance, in terms of ecological benefits for the receiving water, can be achieved using a treatment train incorporating permeable paving and bioretention systems. The review identifies existing research gaps and emphasises the pertinent management practices as well as design issues which are relevant to the mitigation of car park pollution.

Impacts of seasonal activities and traffic conditions on the contamination and accumulation of gully pot sediments: Metal(loid)s and organic substances.

Gully pots (GPs) are an integral urban drainage component, transferring surface runoff into piped systems and reducing sediment and contaminant load on downstream sewers and receiving waters. Sediment build-up in GPs impairs their hydraulic performance, necessitating maintenance for hydraulic function recovery. The variations in sediment accumulation rates between GPs suggested by earlier studies challenge the effectiveness of adopting a generalised maintenance frequency. This study addresses the knowledge gap regarding how various factors influence sediment contamination in GPs. The impacts of seasonal activities and traffic conditions on the contamination of sediments in 27 GPs in areas with varying traffic intensities and street features (roundabouts, intersections, and straight roads) were examined. Over one year, GPs were emptied twice, with sediments collected for winter-spring and summer-autumn accumulation periods. These sediments were analysed for 84 substances, including metal(loid)s, hydrocarbons, polycyclic aromatic hydrocarbons (PAHs), alkylphenols, phthalates, per- and polyfluoroalkyl substances and organotins. Significant temporal changes were identified in key parameters such as electrical conductivity, total organic carbons, tungsten (W), heavy-molecular-weight PAHs (PAH-H) and diisodecyl phthalate (DIDP) in GP sediments, influenced by winter road safety measures and autumn leaf abscissions. Significantly higher concentrations of 4-tert-octylphenol, DIDP, diisononyl phthalate, antimony and W were identified in GP sediments from roundabouts compared to those at the other two street features, exclusively during the winter-spring period. This is attributed to the synergistic effect of winter road safety measures and stop-and-go traffic patterns at roundabouts. No consistent spatial and temporal patterns were identified for substance concentration and mass accumulation rates. Results underscore the potential to develop a prioritisation-based maintenance strategy as an opportunity to enhance the efficiency of GP maintenance operations, ensuring better resource allocation and reduced environmental impact.

Making waves: The NORMAN antibiotic resistant bacteria and resistance genes database (NORMAN ARB&ARG)-An invitation for collaboration to tackle antibiotic resistance.

With the global concerns on antibiotic resistance (AR) as a public health issue, it is pivotal to have data exchange platforms for studies on antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs) in the environment. For this purpose, the NORMAN Association is hosting the NORMAN ARB&ARG database, which was developed within the European project ANSWER. The present article provides an overview on the database functionalities, the extraction and the contribution of data to the database. In this study, AR data from three studies from China and Nepal were extracted and imported into the NORMAN ARB&ARG in addition to the existing AR data from 11 studies (mainly European studies) on the database. This feasibility study demonstrates how the scientific community can share their data on AR to generate an international evidence base to inform AR mitigation strategies. The open and FAIR data are of high potential relevance for regulatory applications, including the development of emission limit values / environmental quality standards in relation to AR. The growth in sharing of data and analytical methods will foster collaboration on risk management of AR worldwide, and facilitate the harmonization in the effort for identification and surveillance of critical hotspots of AR. The NORMAN ARB&ARG database is publicly available at: https://www.norman-network.com/nds/bacteria/.

Impacts of stormwater pipe materials and pipe repairs on stormwater quality: a review.

The water quality implications of transferring stormwater through pipes composed of concrete (new and used), polyvinyl chloride (PVC), galvanized corrugated steel (GCS), high-density polyethylene (HDPE), and pipes subjected to cured in place pipe (CIPP) and spray in place pipe (SIPP) trenchless repair technologies on stormwater quality are reviewed. Studies involve either the use of flowing water or an immersion experimental design, with data showing contact with pipe materials can affect stormwater quality parameters including pH, electrical conductivity (EC), and concentrations of minerals, metals, and organic constituents, e.g. styrene. 'In-transport' changes in pH (1-3 units), EC (2-3-fold), bicarbonate (3-44-fold), and calcium (2-17-fold) in stormwaters were reported following exposure to concrete pipes. Differences between the use of synthetic and field-collected stormwater were identified, e.g. turbidity levels in field-collected stormwater reduced on passage through all pipe types, compared to synthetic water where levels of turbidity on exposure to concrete and cement-based SIPP increased slightly. Transfer through PVC and HDPE pipes had minimal effects on physicochemical parameters, whereas exposure to galvanized corrugated steel pipes led to increases in EC, Zn, and Pb. Though limited data was available, the use of CIPP repairs and associated waste condensate generated during thermal curing and/or incomplete curing of resins was identified to release organic contaminants of concerns (e.g. styrene, vinylic monomers, dibutyl phthalate (DBP), diethyl phthalate (DEP), and benzaldehyde). The implications of findings for both future research and stakeholders with responsibility for reducing diffuse pollution loads to receiving waters are considered.

Accumulation and contamination of gully pot sediments from varied land-use types: metal loads, concentrations and speciation.

Urban stormwater typically enters sewer networks through gully pots, which allow a primary sedimentation of solids upstream of the piped network. The regular removal and disposal of retained sediment are necessary, costly and can involve environmental risks due to the contamination of sediments with substances from the urban environment such as metals. The concentrations and speciation of Cd, Cr, Cu, Ni, Pb and Zn were analysed in sediments from 26 gully pots located in different land use areas in Stockholm, Sweden. In addition, accumulation rates of both sediment and metal masses were evaluated, providing a basis for optimising maintenance practices and better understanding of impacts of characteristic urban land use types. Metal concentrations varied by at most a factor of eight between samples and were always below Swedish polluted site guidelines for less sensitive land use, with only eight samples exceeding the guideline values for Cu and Zn for sensitive land use. Sequential extraction showed Pb and Zn to be the most mobile metals. Sediment accumulation rates varied from 0.003 to 0.197 kg/m2 impermeable surface/year. Metal accumulation rates were much more variable than metal concentrations, with a factor of up to 172 between the highest and lowest rates and the highest metal accumulation rates corresponding to the lower range of mass loads in road runoff. Differences in metal concentrations, sediment or metal mass accumulations could not be solely attributed to either traffic or catchment land use. In contrast, traction grit used for winter road maintenance, which has low (but detectable) metal concentrations, is identified as a major component of gully pot sediments, with a combined effect of both moderating metal concentrations and contributing to total mass.

A study of 101 organic substances in gully pot sediments accumulated over a one-year period in Stockholm, Sweden.

Stormwater runoff is a key pathway for diffuse pollutants to enter receiving waters. Mitigating measures include pollutant substitution, restricting their release into the urban technosphere and limiting the (re-)mobilisation of substances to minimise their negative impacts on receiving waters. Gully pots (GPs) are one of the most ubiquitous urban drainage infrastructure components, providing both a drainage function and limiting the onward transport of pollutants through in-pot sedimentation processes. In this study, sediments accumulated over a one-year period were collected from 26 GPs in catchments of four land-use types in Stockholm, Sweden. Sediments were analysed for 101 organic substances from eight substance groups (hydrocarbons, polycyclic aromatic hydrocarbons, alkylphenols, polychlorinated biphenyls, phthalates, organotins, per- and polyfluoroalkyl substances and brominated fire retardants) to inform an assessment of their occurrence and net accumulation rates over a typical unit operation period. A total of 63 substances were quantified in at least one GP, with aliphatic hydrocarbons, phthalates and organotins quantified in all GP sediments, highlighting their ubiquitous use. The identification of 14 and 21 organic substances in two pedestrian/bike path GPs emphasise the contribution of non-vehicular sources to diffuse pollutant loads. Significantly higher mass accumulation rates of 4-tert-octylphenol, 4-nonylphenols, formaldehyde, dioctyltin and dibutyltin are identified in commercial catchment GPs suggesting the need to enhance source-tracing and runoff quality-control measures within catchments of this land-use type. Sediments in 25 GPs were identified with at least one substance exceeding toxicology-based threshold values, highlighting the runoff quality-control function of GPs in reducing the potential ecotoxic impacts on recipients.

Wastewater monitoring for detection of public health markers during the COVID-19 pandemic: Near-source monitoring of schools in England over an academic year.

BACKGROUND: Schools are high-risk settings for infectious disease transmission. Wastewater monitoring for infectious diseases has been used to identify and mitigate outbreaks in many near-source settings during the COVID-19 pandemic, including universities and hospitals but less is known about the technology when applied for school health protection. This study aimed to implement a wastewater surveillance system to detect SARS-CoV-2 and other public health markers from wastewater in schools in England. METHODS: A total of 855 wastewater samples were collected from 16 schools (10 primary, 5 secondary and 1 post-16 and further education) over 10 months of school term time. Wastewater was analysed for SARS-CoV-2 genomic copies of N1 and E genes by RT-qPCR. A subset of wastewater samples was sent for genomic sequencing, enabling determination of the presence of SARS-CoV-2 and emergence of variant(s) contributing to COVID-19 infections within schools. In total, >280 microbial pathogens and >1200 AMR genes were screened using RT-qPCR and metagenomics to consider the utility of these additional targets to further inform on health threats within the schools. RESULTS: We report on wastewater-based surveillance for COVID-19 within English primary, secondary and further education schools over a full academic year (October 2020 to July 2021). The highest positivity rate (80.4%) was observed in the week commencing 30th November 2020 during the emergence of the Alpha variant, indicating most schools contained people who were shedding the virus. There was high SARS-CoV-2 amplicon concentration (up to 9.2x106 GC/L) detected over the summer term (8th June - 6th July 2021) during Delta variant prevalence. The summer increase of SARS-CoV-2 in school wastewater was reflected in age-specific clinical COVID-19 cases. Alpha variant and Delta variant were identified in the wastewater by sequencing of samples collected from December to March and June to July, respectively. Lead/lag analysis between SARS-CoV-2 concentrations in school and WWTP data sets show a maximum correlation between the two-time series when school data are lagged by two weeks. Furthermore, wastewater sample enrichment coupled with metagenomic sequencing and rapid informatics enabled the detection of other clinically relevant viral and bacterial pathogens and AMR. CONCLUSIONS: Passive wastewater monitoring surveillance in schools can identify cases of COVID-19. Samples can be sequenced to monitor for emerging and current variants of concern at the resolution of school catchments. Wastewater based monitoring for SARS-CoV-2 is a useful tool for SARS-CoV-2 passive surveillance and could be applied for case identification and containment, and mitigation in schools and other congregate settings with high risks of transmission. Wastewater monitoring enables public health authorities to develop targeted prevention and education programmes for hygiene measures within undertested communities across a broad range of use cases.

An evaluation of temporal changes in physicochemical properties of gully pot sediments.

Diffuse pollution is recognised as a major challenge in achieving EU Water Framework Directive compliance, with urban runoff being a key pathway connecting various sources to receiving waters. Gully pots, as one of the ubiquitous urban drainage infrastructures, are placed at the inlets of piped drainage pipe network and actively drain runoff from urban catchment with suspended solids proportionally retained. The physiochemical properties of these retained solids reflect the activities within the catchment during the accumulation period. In this work, seven gully pots in two catchment types (highway and housing) in Luleå, Sweden were fully emptied and sediments analysed for total mass, particle size distribution and selected metal concentrations by six size fractions. The results of this sampling campaign are compared with the results of a 2005 study of the same gully pots to identify changes in the physicochemical properties of sediments over time and examine whether changes identified can be linked to changes in wider catchment management practices. The results highlight the potential impacts of winter road maintenance operations (e.g. up to a 15-fold higher solids loading rate in road catchment gully pots), reaching a normalised solids accumulation rate of 0.176-0.819 kg m2 year-1. An increase in tyre and road wear associated with winter road maintenance operations is also understood to contribute to the temporal increase of several metals including Cu, Zn, Co, Cr and V in the < 63-µm solids fraction in the road catchment gully pots. The concentrations of As and Pb decrease in all size fractions in both catchments, with the implementation of unleaded fuels (for Pb in housing catchment only), End-of-Life Vehicle Directive (Directive 2000/53/EC) (for Pb in both catchments), and strengthened industrial emission reduction measures suggested as possible drivers. The high contamination load for Zn, Cu, Cd and Pb in < 63-µm sediments from low-traffic housing catchment also emphasised the necessity of tracing and restricting non-traffic-related metal sources. Further seasonal monitoring of gully pot sediments is recommended to fully follow up the development of metals loading in both catchments.

Development and application of an innovative approach to predicting pollutant concentrations in highway runoff.

Recognising the challenges and limitations of current methodologies to predict highway runoff concentrations, this paper presents a novel approach based on the derivation of pollutant emission factors for twelve different types of vehicle. Published emission factor data and properties of differing vehicles types are combined with annual average daily traffic volume (AADT), highway characteristics and rainfall data to determine the pollutant distributions associated with differing highway and traffic types. In this paper, the method is applied to 126 sections of highway in the Greater London Borough of Enfield (United Kingdom; UK) and results are comparable with values reported in the literature. The approach is used to identify the level of AADT predicted to result in an exceedance of environmental quality standards (EQS), with results suggesting that runoff from highways experiencing AADT values as low as 5000 may require treatment prior to discharge to receiving waters. Future scenario analyses indicate that the impact of progressively replacing petrol and diesel vehicles with electric vehicles will have negligible impact on concentrations of zinc (Zn), copper (Cu), cadmium (Cd) and total suspended solids discharging from highway environments. The approach enables identification and ranking of urban highways in terms of their pollution runoff potential and provides an important support to users in prioritising locations for the installation of sustainable drainage options in order to protect receiving water environments.

Multiclass target analysis of contaminants of emerging concern including transformation products, soil bioavailability assessment and retrospective screening as tools to evaluate risks associated with reclaimed water reuse.

The occurrence of 200 multiclass contaminants of emerging concern (CECs) encompassing 168 medicinal products and transformation products (TPs), 5 artificial sweeteners, 12 industrial chemicals, and 15 other compounds was investigated in influent and effluent wastewater samples collected during 7 consecutive days from 5 wastewater treatment plants (WWTPs) located in Cyprus. The methodology included a generic solid-phase extraction protocol using mixed-bed cartridges followed by Ultra-High Performance Liquid Chromatography coupled with Quadrupole-Time of Flight Mass Spectrometry (UHPLC-QTOF-MS) analysis. A total of 63 CECs were detected at least in one sample, with 52 and 55 out of the 200 compounds detected in influents and effluents, respectively. Ten (10) out of the 24 families of parent compounds and associated TPs were found in the wastewater samples (influent or effluent). 1-H-benzotriazole, carbamazepine, citalopram, lamotrigine, sucralose, tramadol, and venlafaxine (>80 % frequency of appearance in effluents) were assessed with respect to their bioavailability in soil as part of different scenarios of irrigation with reclaimed water following a qualitative approach. A high score of 12 (high probability) was predicted for 2 scenarios, a low score of 3 (rare occasions) for 2 scenarios, while the rest 28 scenarios had scores 5-8 (unlikely or limited possibility) and 9-11 (possibly). Retrospective screening was performed with the use of a target database of 2466 compounds and led to the detection of 158 additional compounds (medicinal products (65), medicinal products TPs (15), illicit drugs (7), illicit drugs TPs (3), industrial chemicals (11), plant protection products (25), plant protection products TPs (10), and various other compounds (22). This work aspires to showcase how the presence of CECs in wastewater could be investigated and assessed at WWTP level, including an expert-based methodology for assessing the soil bioavailability of CECs, with the aim to develop sustainable practices and enhance reclaimed water reuse.

Monitoring occurrence of SARS-CoV-2 in school populations: A wastewater-based approach.

Clinical testing of children in schools is challenging, with economic implications limiting its frequent use as a monitoring tool of the risks assumed by children and staff during the COVID-19 pandemic. Here, a wastewater-based epidemiology approach has been used to monitor 16 schools (10 primary, 5 secondary and 1 post-16 and further education) in England. A total of 296 samples over 9 weeks have been analysed for N1 and E genes using qPCR methods. Of the samples returned, 47.3% were positive for one or both genes with a detection frequency in line with the respective local community. WBE offers a low cost, non-invasive approach for supplementing clinical testing and can provide longitudinal insights that are impractical with traditional clinical testing.

Development of a qualitative approach to assessing risks associated with the use of treated wastewater in agricultural irrigation.

The European Commission's draft regulation for minimum requirements for water reuse in agriculture addresses microbial and basic water quality parameters but does not consider the potential impacts of chemicals of emerging concern (CECs) on human and environmental health. Because insufficient data prevents the quantitative characterisation of risks posed by CECs in treated wastewater (TWW), this paper presents a framework, which combines data and expert judgement to assess likelihood of occurrence and magnitude of impact. An increasing relative scale is applied where numeric values are pre-defined to represent comparative levels of importance. Subsequently, an overall assessment of the level of risk is characterised by multiplying together allocated scores, to obtain a single discrete overall score per CEC. Guidelines to support implementation of the framework as far as soil (the initial receiving compartment and pathway to further protected targets) are developed and applied. The approach is demonstrated through its application to clarithromycin, where results indicate that - under the considered scenario - there is limited possibility of its occurrence in soil in a bioavailable form. The role of a qualitative risk assessment approach is considered and the opportunity for its outputs to inform future research agendas described.

Detection of SARS-CoV-2 RNA in the Danube River in Serbia associated with the discharge of untreated wastewaters.

In Serbia less than 13% of collected municipal wastewaters is being treated before their release in the environment. This includes all municipal wastewater discharges from Belgrade (capital city of Serbia; population 1,700,000). Previous research has identified the impacts of raw wastewater discharges from Belgrade on the Danube River, and this study investigated if such discharges also provided a pathway for SARS-CoV-2 RNA material. Samples were collected during the most critical circumstances that occurred so far within the COVID-19 pandemics in Serbia. Grab and composite samples were collected in December 2020, during the peak of the third wave (in terms of reported cases) at the site which receives the wastewater loads in Belgrade. Grab samples collected upstream and downstream of Belgrade were also analyzed. RNA was quantified using RT-qPCR with primer sets targeting nucleocapsid (N1 and N2) and envelope (E) protein genes. SARS-CoV-2 RNA (5.97 × 103 to 1.32 × 104 copies/L) was detected only in samples collected at the site strongly impacted by the wastewaters where all three applied primer sets gave positive signals. Determined concentrations correspond to those reported in wastewater influents sampled at treatment plants in other countries indicating an epidemiological indicator function of used approach for rivers with high pollution loads in countries with poor wastewater treatment.

Making Waves: Collaboration in the time of SARS-CoV-2 - rapid development of an international co-operation and wastewater surveillance database to support public health decision-making.

The presence of SARS-CoV-2 RNA in wastewater was first reported in March 2020. Over the subsequent months, the potential for wastewater surveillance to contribute to COVID-19 mitigation programmes has been the focus of intense national and international research activities, gaining the attention of policy makers and the public. As a new application of an established methodology, focused collaboration between public health practitioners and wastewater researchers is essential to developing a common understanding on how, when and where the outputs of this non-invasive community-level approach can deliver actionable outcomes for public health authorities. Within this context, the NORMAN SCORE "SARS-CoV-2 in sewage" database provides a platform for rapid, open access data sharing, validated by the uploading of 276 data sets from nine countries to-date. Through offering direct access to underpinning meta-data sets (and describing its use in data interpretation), the NORMAN SCORE database is a resource for the development of recommendations on minimum data requirements for wastewater pathogen surveillance. It is also a tool to engage public health practitioners in discussions on use of the approach, providing an opportunity to build mutual understanding of the demand and supply for data and facilitate the translation of this promising research application into public health practice.

Recommendations to derive quality standards for chemical pollutants in reclaimed water intended for reuse in agricultural irrigation.

The reuse of treated municipal wastewater (herein referred to as reclaimed water) in agricultural irrigation (RWAI) as a means to alleviate water scarcity is gaining increasing policy attention, particularly in areas where water demand mitigation measures have proved insufficient. However, reclaimed water reuse in practice is lagging behind policy ambition, with <2.5% of it reused in a European context. A key barrier identified as limiting its full valorisation is concern over its impact on human and environmental health. To address this concern, and to meet further objectives including achieving parity between current reclaimed water reuse guidelines operational in various Member States, the European Commission has proposed a regulation which identifies minimum quality requirements (MQR) for a range of microbiological and physico-chemical parameters but the inclusion of compounds of emerging concern (CECs) in terms of the determination of quality standards (QS) is missing. This paper reviews the existing pertinent EU legislation in terms of identifying the need for CEC QS for RWAI, considering the scope and remit of on-going pan-European chemicals prioritisation schemes. It also evaluates opportunities to link in with the existing EQS derivation methodology under the EU WFD to address all protection targets in the environmental compartments exposed via potential pathways of RWAI. Finally, it identifies the main data gaps and research needs for terrestrial ecosystems, the removal efficiency of CECs by WWTPs and transformation products generated during the wastewater reuse cycle.