Zonation of bulk and rhizosphere soil bacterial communities and their covariation patterns along the elevation gradient in riparian zones of three Gorges reservoir, China.

Zonation is a typical pattern of soil distribution and species assembly across riparian habitats. Microorganisms are essential members of riparian ecosystems and whether soil microbial communities demonstrate similar zonation patterns and how bulk and rhizosphere soil microorganisms interact along the elevation (submergence stress) gradient remain largely unknown. In this study, bulk and rhizosphere (dominant plant) soil samples were collected and investigated across riparian zones where the submergence stress intensity increased as the elevation decreased. Results showed that the richness of bacterial communities in bulk and rhizosphere soil samples was significantly different and presented a zonation pattern along with the submergence stress gradient. Bulk soil at medium elevation that underwent moderate submergence stress had the most abundant bacterial communities, while the species richness of rhizobacteria at low elevation that experienced serious submergence stress was the highest. Additionally, principal coordinate analysis (PCoA) and significance tests showed that bulk and rhizosphere soil samples were distinguished according to the structure of bacterial communities, and so were bulk or rhizosphere soil samples from different elevations. Redundancy analysis (RDA) and Mantel test suggested that bacterial communities of bulk soil mainly relied on the contents of soil organic matter, total carbon (TC), total nitrogen (TN), sodium (Na), calcium (Ca) and magnesium (Mg). Contrastingly, the contents of Na and Mg were the main factors explaining the variation in rhizobacterial community composition. Correlation and microbial source tracking analyses showed thatthe relationship of bulk and rhizosphere soil bacteria became much stronger, and the rhizosphere soil may get more bacterial communities from bulk soil with the increase in submergence severity. Our results suggest that the abiotic and biotic components of the riparian ecosystem are closely covariant along the submergence stress gradient and imply that the bacterial community may be a key node linking soil physiochemical properties and vegetation communities.

Reservoir NO3- pollution and chemical weathering: by dual isotopes of δ15N-NO3-, δ18O-NO3- and geochemical constraints.

Reservoir dams alter the nutrient composition and biogeochemical cycle. Thus, dual isotopes of δ18O-NO3- and δ15N-NO-3 and geochemical signatures were employed to study the NO3- pollution and chemical weathering in the Three Gorges Reservoir (TGR), China. This study found that the TGR dam alters the δ15N-NO3- composition and is enriched in the recharge period. Values of δ15N-NO3- varied from 4.5 to 12.9‰ with an average of 9.8‰ in the recharge period, while discharge period δ15N-NO3- ranged from 3.2 to 12.5‰, with an average of 9.3‰. δ18O-NO3- varies (1.2-11.3‰) with an average of 6.5‰ and (2.4-12.4‰) with an average of 7.5‰, in the recharge and discharge periods, respectively. Stable isotopic values sharply decreased from upstream to downstream, indicating the damming effects. δ18O-NO3- and δ15N NO3- confirm that sewage effluents, nitrification of soil organic material, and NH4+ fertilizers were the primary sources of NO3- in the reservoir. Carbonate weathering mainly provides ions to the reservoir. HCO3- + SO42- and Ca2+ + Mg2+ represent 90% of major ions in the TGR. Downstream sampling sites showed low solute concentration during the recharge period, indicating the dam effect on solute concentration. Ca-Mg-Cl-, Ca-HCO3- and Ca-Cl- were the main water types in the TGR. The average percentage of solutes contribution revealed the carbonate weathering, evaporites dissolution, silicate weathering, and atmospheric input were 51.9%, 41%, 7.8%, and 1.7% for the recharge period. In contrast, the discharge period contributed 66.4%, 29.2%, 10%, and 4.3%, respectively. TGR water is moderately suitable for irrigation, and hardness is high in drinking water. This study provides new insight into the dual isotopic approach and geochemical signatures to interpret the NO3- cycle and chemical weathering process under dam effects in the TGR. However, this isotopic application has some limitations in source identification, isotope fractionation, and transformation mechanisms of nitrate. Thus, further studies need to be done on these topics for a better undestanding.

Mercury contamination in the riparian ecosystem during the reservoir discharging regulated by a mega dam.

Mercury (Hg) is extremely poisonous and can be absorbed through touch, inhalation, or consumption. In the living environment, Hg in contaminated sediment can be transferred into grass by the direct absorption through the roots or shoots. The intake of Hg due to Hg emissions may pose a threat to living bodies especially to human beings. The present study aims to provide a novel insight about total mercury (THg) and methyl mercury (MeHg) in a riparian grass (Cynodon dactylon (L).Pers) and sediments during the discharging phase (summertime at 145 m water level) in Three Gorges Reservoir (TGR-China); where C. dactylon is a dominant perennial herb in the riparian zone. Yet, the potential risk of Hg contamination in the riparian ecosystem is not thoroughly assessed in the dam regulated reservoir. This study was conducted in the riparian zones of the reservoir formed by a mega dam (Three Gorge Dam) which regulates the water levels during the summer and winter period in the TGR. Our results showed that riparian sediments were acting as a sink for THg and MeHg. Insignificant correlation of THg and MeHg was found between the amphiphyte C. dactylon and its surrounding sediments in the TGR. Bioconcentration factors values for MeHg were found higher than 1 in all study locations in the riparian zones in TGR, which could be due to action of certain bacteria/purely chemical-based methylation on inorganic form of Hg. Additionally, translocation factor indices also highlighted that the amphiphyte C. dactylon was MeHg accumulator in riparian zones. These results suggested that since riparian sediment was found acting as the sink for THg and MeHg during discharging phase, MeHg contamination in the amphiphyte C. dactylon in riparian zones was not caused by the riparian sediments but by other factors, for instance, the anthropogenic activities in the TGR. Finally, this study leads to conclude that amphiphyte C. dactylon can be used as biomonitoring agent for Hg pollution in the TGR.

Plant Morphological, Physiological and Anatomical Adaption to Flooding Stress and the Underlying Molecular Mechanisms.

Globally, flooding is a major threat causing substantial yield decline of cereal crops, and is expected to be even more serious in many parts of the world due to climatic anomaly in the future. Understanding the mechanisms of plants coping with unanticipated flooding will be crucial for developing new flooding-tolerance crop varieties. Here we describe survival strategies of plants adaptation to flooding stress at the morphological, physiological and anatomical scale systemically, such as the formation of adventitious roots (ARs), aerenchyma and radial O2 loss (ROL) barriers. Then molecular mechanisms underlying the adaptive strategies are summarized, and more than thirty identified functional genes or proteins associated with flooding-tolerance are searched out and expounded. Moreover, we elaborated the regulatory roles of phytohormones in plant against flooding stress, especially ethylene and its relevant transcription factors from the group VII Ethylene Response Factor (ERF-VII) family. ERF-VIIs of main crops and several reported ERF-VIIs involving plant tolerance to flooding stress were collected and analyzed according to sequence similarity, which can provide references for screening flooding-tolerant genes more precisely. Finally, the potential research directions in the future were summarized and discussed. Through this review, we aim to provide references for the studies of plant acclimation to flooding stress and breeding new flooding-resistant crops in the future.

Different Recovery Processes of Soil Ammonia Oxidizers from Flooding Disturbance.

Understanding how microorganisms respond to environmental disturbance is one of the key focuses in microbial ecology. Ammonia-oxidizing bacteria (AOB) and archaea (AOA) are responsible for ammonia oxidation which is a crucial step in the nitrogen cycle. Although the physiology, distribution, and activity of AOA and AOB in soil have been extensively investigated, their recovery from a natural disturbance remains largely unknown. To assess the recovery capacities, including resistance and resilience, of AOA and AOB, soil samples were taken from a reservoir riparian zone which experienced periodically water flooding. The samples were classified into three groups (flooding, recovery, and control) for a high-throughput sequencing and quantitative PCR analysis. We used a relative quantitative index of both the resistance (RS) and resilience (RL) to assess the variation of gene abundance, alpha-diversity, and community composition. The AOA generally demonstrated a better recovery capability after the flooding disturbance compared to AOB. In particular, AOA were more resilient after the flooding disturbance. Taxa within the AOA and AOB showed different RS and RL values, with the most abundant taxa showing in general the highest RS indices. Soil NH4+ and Fe2+/Fe3+ were the main variables controlling the key taxa of AOA and AOB and probably influenced the resistance and resilience properties of AOA and AOB communities. The distinct mechanisms of AOA and AOB in maintaining community stability against the flooding disturbance might be linked to the different life-history strategies: the AOA community was more likely to represent r-strategists in contrast to the AOB community following a K-life strategy. Our results indicated that the AOA may play a vital role in ammonia oxidation in a fluctuating habitat and contribute to the stability of riparian ecosystem.

Effects of riparian pioneer plants on soil aggregate stability: Roles of root traits and rhizosphere microorganisms.

Pioneer plants are vital in stabilizing soil structure while restoring reservoir drawdown areas. However, uncertainties persist regarding the mechanism of pioneer plants to soil stability in these delicate ecosystems. This study aims to unravel the plant-soil feedback mechanisms from the roles of root traits and rhizosphere microorganisms. We conducted a mesocosm experiment focusing on four common pioneer plants from the drawdown area of Three Gorges Reservoir, China. Using the wet sieving methodology, trait-based approach and high-throughput sequencing technology, we explored soil aggregate stability parameters, plant root traits and rhizosphere microbial communities in experimental plant groups. The interacting effect of pioneer plant species richness, root traits, and rhizosphere microbial communities on soil aggregate stability was quantified by statistical and machine-learning models. Our results demonstrate that diverse pioneer plant communities significantly enhance soil aggregate stability. Notably, specific species, such as Cynodon dactylon (L.) Pers. and Xanthium strumarium L., exert a remarkably strong influence on soil stability due to their distinctive root traits. Root length density (RLD) and root specific surface area (RSA) were identified as crucial root traits mediating the impact of plant diversity on soil aggregate stability. Additionally, our study highlights the link between increased rhizosphere fungal richness, accompanied by plant species richness, and enhanced soil aggregate stability, likely attributable to elevated RLD and RSA. These insights deepen our understanding of the role of pioneer vegetation in soil structure and stability, providing valuable implications for ecological restoration and management practices in reservoir drawdown areas.

Diminished storage capacity of ponds caused by sedimentation weakens their nitrogen removal efficiency.

Though their small size, ponds play a disproportionately crucial role in eliminating nitrogen (N) transporting to downstream freshwaters. As significant water infrastructures, ponds are non-sustainable due to loss of storage capacity resulting from sedimentation. However, the effects of pond sedimentation on N removal is widely neglected in landscape N processing. The NUFER (Nutrient flows in Food chains, Environment and Resources use) model was employed to estimate N runoff from 1960 to 2018. We reconstructed the dynamic of number and storing capacity of about 14 million ponds due to construction and sedimentation from 1960 to 2018, projecting these trends into the year 2060. Our approach incorporated first-order kinetic reactions, including water residence time (HRT), to estimate N removal of ponds, utilizing data 6 monitoring ponds and 81 ponds from literature studies. Our analysis reveals a fourteen-fold increase in N runoff over the past six decades, rising from 0.8 Mt N in 1960 to 11.4 Mt N in 2018. Due to the initial rapid expansion of ponds, N removal by ponds increased from 6.4 % in 1960 to 13.6 % in 1990. Sedimentation is prevalent in ponds, particularly in small ponds with a sedimentation accumulation rate of 2.96 cm yr-1. Pond sedimentation, which reduces HRT, resulted in a decrease in pond N removal percentage to 11.2 % in 2018 and a projected 7.4 % by the year 2060, assuming similar sediment accumulation rates persist in the future. Overall, our findings underscore the non-negligible role of ponds as landscape nodes in N cycling. Urgent mitigation measures are needed to extend the lifetime of existing ponds and sustain their critical role in water quality management.

Soil Nitrogen and Flooding Intensity Determine the Trade-Off between Leaf and Root Traits of Riparian Plant Species.

The investigation into trade-offs among plant functional traits sheds light on how plants strategically balance growth and survival when facing environmental stress. This study sought to evaluate whether trade-offs observed at both community and individual species levels could indicate adaptive fitness across an intensity of flooding intensity. The study was conducted at 25 sampling sites spanning approximately 600 km along the riparian zone in the Three Gorges Reservoir area, China. The findings revealed that, along the flooding gradient, the overall riparian community did not exhibit significant trade-offs between leaf and root traits. Examining three broadly distributed dominant species (Cynodon dactylon, Xanthium strumarium, and Abutilon theophrasti), perennial plants showed pronounced trade-offs under low flooding intensity, while annuals exhibited trade-offs under moderate and low flooding intensity. The trade-offs were evident in traits related to nitrogen-carbon resources, such as specific leaf area, root tissue density, and photosynthetic rate. However, under strong flooding intensity, the relationship between leaf and root traits of the species studied was decoupled. Furthermore, the study identified a significant correlation between soil nitrogen and the trade-off traits under moderate and low flooding intensity. Integrating results from the CSR (Competitors, Stress-tolerators, Ruderals) strategy model, species niche breath analysis, and nitrogen-regulated trade-off, the study revealed that, in the face of high flooding intensity, perennial species (C. dactylon) adopts an S-strategy, demonstrating tolerance through a conservative resource allocation that decouples leaf-root coordination. Annual species (X. strumarium and A. theophrasti), on the other hand, exhibit niche specialization along the flooding gradient, employing distinct strategies (R- and C-strategy). As flooding stress diminishes and soil nitrogen level decreases, plant strategies tend to shift towards an R-strategy with a competition for reduced N resources. In conclusion, the study highlighted the pivotal roles of soil nitrogen and flooding intensity acting as the dual determinants of species growth and tolerance. These dynamics of growth-tolerance balance were evident in the diverse trade-offs between leaf and root traits of individual plant species with different life histories, underscoring the array of adaptive strategies employed by riparian plants across the flooding intensity gradient.

First insights into diversity and potential metabolic pathways of bacterial and fungal communities in the rhizosphere of Argemonemexicana L. (Papaveraceae) from the water-level-fluctuation zone of Wudongde Reservoir of the upper Yangtze river, China.

The water-level fluctuation zone (WLFZ) of Wudongde reservoir of the upper Yangtze river is a completely new aquatic-terrestrial transitional zone, and its plant degenerate issue is attracting global concerns. Uncovering the unknown rhizosphere microbiome of dominant plants of this zone is helpful in understanding the plant-microbe interactions and their growth under the largely varying environment. Here, a first exploration of the rhizosphere bacterial and fungal communities of wilted (JB) and unwilted (JA) Argemonemexicana L. individuals from the WLFZ of Wudongde reservoir was carried out using high-throughput sequencing and MetaCyc metabolic pathway analyses. The results showed that rhizosphere of wilted A.mexicana L individuals exhibited a higher microbial richness and diversity than the unwilted ones, irrespective of the bacterial and fungal communities. It was noted that 837 common bacterial amplicon sequence variants (ASV) and 92 common fungal ASV were presented in both JA and JB with 3108 bacteria and 212 fungi unique to JA, and 3569 bacteria and 693 fungi unique to JB. Linear discriminant analysis effect Size (LEfSe) analyses indicated that the taxa that had the most contribution to observed differences between both JA and JB was Proteobacteria, Actinobacteria and Ascomycota for JA, and Bacteroidetes, Firmicutes, Verrucomicrobia, Basidiomycota and Ascomycota for JB. Organic compound conversion pathway (degradation/reduction/oxidation) was consistently highly represented in the rhizosphere microbiomes of both JA and JB. Overall, this study provides insights into the rhizosphere microbiome composition, diversity and metabolic pathways of both wilted and unwilted A.mexicana L. individuals in the WLFZ of Wudongde reservoir, and the results give valuable clues for manipulating microbes to support plant growth in such a recently-formed WLFZ under a dry-hot valley environment.

Dynamic of riverine pCO2, biogeochemical characteristics, and carbon sources inferred from δ13C in a subtropical river system.

Rivers significantly contribute to the global carbon budget, but data limitations and uncertainty are hampered by CO2 quantification in the global rivers. Thus, this study estimated riverine pCO2 by employing the pH-alkalinity-temperature method, and dissolved inorganic (DIC), dissolved organic (DOC), particulate organic (POC) carbon, and their isotopes (δ13C) with Chlorophyll-a (Chl a) were measured in river water samples from 26 sampling sites for characterization and source identification in the Yangtze River system. The estimated pCO2 varies from (120 ppm) to (3400 ppm) with an average (1085 ppm) across the Yangtze River and pCO2 is almost three times oversaturated than the ambient air (380 ppm). The downstream sites pronounced elevated pCO2 than the upstream sites. The relationship of δ13CDIC and pCO2 indicated that pCO2 control is seasonally independent. The significant correlations between DOC, POC, and pCO2 revealed that organic carbon influenced pCO2 in the river. The seasonal fluctuations of pCO2 were observed with an average of (762.23 ppm) and (1407.35 ppm) in winter and summer, respectively. δ13CDIC showed that the metabolic process has a negligible influence on DIC, δ13CDIC, and pCO2. δ13CDIC values increased from -8.95‰ to -4.91‰ during summer, whereas winter increased from -19.76‰ to -1.97‰ suggesting that DIC derived from carbonate weathering, dissolution of atmospheric and soil CO2. The δ13CDOC (-30.43‰ to -24.05‰) and δ13CPOC (-29.87‰ to -23.37‰) values confirmed that organic carbon mainly derived from the degradation of organic materials in soil. δ13CDIC revealed that anthropogenic sewage discharge slightly modified DIC composition. Overall, this study provides new insight into recent seasonal fluctuations of the pCO2, DOC, POC, DIC, δ13C, and their inputs. Thus, these variations and inputs of carbon transported by the Yangtze River could have a significant influence not only on the biogeochemical cycle and ecosystem process but also on the global carbon budget.

Heavy metal toxicity, ecological risk assessment, and pollution sources in a hydropower reservoir.

Heavy metal (HM) toxicity, ecological risk, and pollution sources were analyzed using the pollution indexing and statistical methods in the Three Gorges Reservoir (TGR). The average concentration of HM increased in the order of Cr < Ni < As < Cd < Cu < Mn < Pb < Zn < Al < Fe during the recharge period and Cd < Cr < Ni < As < Cu < Pb < Mn < Zn < Al < Fe during the discharge period. Significant spatial variations of Pb, Zn, Cd, As, Mn, Ni, Cr, and Cu were observed at the upstream and downstream sampling sites. Pb sharply increased during the recharge period, ranges (6.93 -148.62 µg/L) and exceeded WHO and USPEA standards limit. HPI, HEI, Cd, WPI indicated low pollution and moderate pollution with the strong influence of Pb and Cd in the discharge and recharging period, respectively. HTML values are below the permissible toxicity load except for Pb. The Pb toxicity removal percentage is 56.47%, suggesting that the lead's toxicity level is high in TGR and requires the removal process. Ecological risk index values indicated that pollution is low in TGR. The potential ecological risk indexes (RI) of 9.07 and 31.60 were obtained for the discharge and recharge period, respectively, indicating low potential ecological risk from heavy metals in TGR. However, RI values revealed that (Pb, Cd, As Cr Ni, Cu Zn, and Mn) were the most ecological risk HMs in TGR. A significant ecological risk of Pb and Cd distribution was observed across the TGR. Multivariate statistical results found that Pb, Cd, Zn, Mn, Ni, As, Cr, Cu mainly originate from industrial wastewater, mining, metals processing, and agricultural runoff. Fe and Al were mainly from bedrock weathering. Pb, Cd, Zn HMs are a threat to the reservoir ecosystem. This study delivered a current status of HM pollution, toxicity, ecological risk, and pollution sources, indicating a vital insight into HM pollution and water security management in the Three Gorges Reservoir.

Shift from soil chemical to physical filters in assembling riparian floristic communities along a flooding stress gradient.

Understanding community assembly is a key issue in recognizing community succession and guiding the restoration of degraded ecosystems. Based on the stress-dominance hypothesis (SDH), along a gradient of increasing environmental stress, the relative importance of environmental filtering is supposed to be dominant but species interaction could be a minor process in assembling communities. However, this hypothesized model of the assembly-rule shift was equivocally supported by various studies. In this study, by examining riparian plant communities with the zonation distribution of species composition along a markedly contrast flooding-stress gradient, a general aim was to clarify whether assembly rules of the communities would be also sorted into the zonation pattern as expected by the SDH. Another aim was to identify how edaphic factors associate with the assembly processes. Firstly, we found that even under the distinct stress gradient, community assembly was not stratified into different rules as the SDH expected, but environmental filtering appeared as a dominant assembly process across the stress gradient. Secondly, although filtering holds as a dominant assembly rule, environmental filters were found different along the gradient. By disentangling the filters of edaphic attributes, we found that the filters significantly shifted from soil physical properties to chemical nutrients governing the filtering process along the gradient. This result revealed that, across the contrast gradient, the environmental deterministic process on assembly is so strong that the other assembly processes became weaker. By synthesizing our results, the SDH may not be applied even under the context of a contrast stress gradient, which suggests that environmental context may be a key in testing and applying the SDH. Finally, in guiding riparian restoration under strong stress, we suggest that soil physical structure rather than chemical nutrients shall be given a priority for consideration in rebuilding the degraded riparian communities.

[Relationship between alien plant invasion and landscape matrix in the water-level fluctuating zone of the Three Gorges Reservoir, China.] / ä¸‰å³¡åº“åŒºæ¶ˆè½å¸¦å¤–æ¥æ¤ç‰©å ¥ä¾µä¸Žæ™¯è§‚åŸºè´¨ç»„æˆç»“æž„çš„å ³è”æ€§.

Invasive process of alien species is affected by not only the invaded habitats, but also the surrounding landscape matrix. Understanding the effects of landscape matrix on alien species is of great significance for controlling invasive alien species. We surveyed plant communities along the water-level fluctuating zone (WLFZ) of the Three Gorges Reservoir. Invasive status of alien plant species was evaluated. Totally 10 spatial scales of the surrounding landscape matrix in the scope of 2000 m (including WLFZ) were classified, and 14 landscape indices were applied to analyze the landscape matrix composition and configuration. Using the principal component analysis and correlation analysis, the effects of landscape matrix on the alien invasive plant species and associated scale effect were tested. Results showed that a total of 42 alien invasive plant species were found in the WLFZ, belonging to 17 families and 36 genera. Fuling was a dividing place to differentiate invasive species distribution. The number of the alien invasive species between Fuling and the Three Gorges Dam was found more than that between Fuling and Jiangjin. For the all scales (within 2000 m). The higher the landscape matrix fragmentation was, the more difficult the alien species invading. The higher landscape connectivity was, the easier the alien species invading. The effects of landscape matrix composition and configuration on the invasive plant diversity at large scales (1200-2000 m) was more significant than those at small scales (200-1000 m), in which landscape matrix composition and configuration at 1200-1400 m showed the strongest effect, demonstrating a significant spatial scale effect. Different invasive plant species showed the scale effects of landscape matrix composition and configuration. At all scales, Xanthium strumarium and Bidens frondosa showed weak correlations with landscape indices, but Bidens tripartita and Erigeron canadensis showed strong correlations. Landscape matrix was closely related to invasive plant species, and demonstrated a significant scale effect. The alien invasive plant species could be traced to the landscape matrix at large scales. Grassland and forest patches at the small scales could be used as the 'stepping stone' for the alien species transiting before they arrived at the WLFZ. In order to control alien plants in the WLFZ, land-use management and optimization should be strengthened at different scales of landscape matrix on the basis of enhancement of habitat management. A diversified comprehensive control for alien species should thus be taken into account.

A higher river sinuosity increased riparian soil structural stability on the downstream of a dammed river.

Hydropower dam constructions and operations have dramatically changed the original hydrological regime of natural rivers. Because of significantly slashed and suspended sediments blocked by damming, discharged "clear" water was found to play a strong undercutting effect on the riverbank and to exacerbate riparian soil erosion on the downstream near dams. Yet, it is still an unsettled issue whether the instability of riparian soil structure would be simply correlated negatively with the distance to a dam. In this study, soils along the downstream riparian zone of a huge dam on the River Yangtze, China, were sampled to examine the distance effect on the riparian soil structural stability. Water-stable aggregates were fractionated by the wet-sieving method. Mean weight diameter (MWD) and geometric mean diameter (GMD) were used to indicate riparian soil stability. Further, the fractal dimension (D) and soil erodibility parameter (K) were used to represent the likelihood of riparian erosion. Our results revealed that riparian soil structural stability demonstrated a high spatial heterogeneity along the River Yangtze, and was less affected by the spatial distance to the dam. Rather, the soil stability was primarily influenced by a river shape index (sinuosity) and local edaphic properties. The river sinuosity index demonstrated a positive relationship with soil structural stability. Additionally, soil organic matter was found as a major edaphic factor in stabilizing soil structure. The results indicated that river sinuosity plays a crucial role in stabilizing soil by accumulating soil organic matters. Our findings implied that the potential negative impact of damming effect on soil stability may be attenuated by maintaining a higher sinuosity of the river. Against the risk of riparian soil erosion along the dammed river, the configuration of river morphology shall be considered as one of the potential managements in offsetting the negative impacts of damming.

Dam-induced difference of invasive plant species distribution along the riparian habitats.

Riparian ecosystem is structurally unstable due to the frequent disturbances from water fluctuation. Moreover, dams on large rivers tend to trigger fundamental changes of the composition and structure of riparian plant communities, which provides high odds for invasive species to colonize. Yet, how the invasive species distribute along a dam-induced riparian habitat, and how the native species resist to plant invasion are still puzzles. In this study, we investigated spatial distribution of invasive floral species and its correlation with the distance from dam and the dam-triggered flooding stresses, as well as the resistance of native species to plant invasion in the water level fluctuation zone (WLFZ) of the Three Gorges Reservoir (TGR) along the Yangtze River. By our investigation, a total of 43 alien plant species belonging to 14 families and 34 genera were found, including 20 existed and 23 newly discovered alien species recorded. Most of the new invasive species are annual herbs of the Asteraceae family. At the current successional stage, the new invasive species had not yet fully occupied the habitats of the existed invasive species. Longitudinally, number and coverages of the new invasive species showed an opposite distribution pattern to the existed invasive species, but vertically they demonstrated similar pattern. Currently, the new dominant invasive species are mainly concentrated at the intermediate elevation of WLFZ in the middle section of the reservoir, whereas the existed dominant invasive species have proliferated across the whole WLFZ. Additionally, native species showed a weak resistance to plant invasion, and water fluctuation along the elevation exerted the most significant influence on plant invasion. The results indicated that, after a decade of riparian community succession, the invasiveness of alien species remain persisted. The potential penetration site of the invasion may locate at the intermediate section along the vertical and longitudinal dimension.

[Anti-inflammatory and analgesic effects of saponins from D. zingiberensis C. H. Wright and diosgenin derivative on mice].

OBJECTIVE: To determine the potential anti-inflammatory and analgesic activities of saponins from D. zingiberensis C. H. Wright and diosgenin derivative on xylene-induced acute ear edema in mice. METHODS: 3beta-2-acetoxy-benzoyl-diosgenin ester (ABDE) was synthesized by acylation of aspirin through two-step of reaction. The analgesic activities of ABDE and saponins were investigated through acetic acid-induced writhing response of the mice. The anti-inflammatory effects of saponins and ABDE were observed on the xylene-induced swelling ears of the mice. RESULTS: The structure of ABDE was confirmed by 1H-NMR and ESI-MS. ABDE had significantly higher anti-inflammatory and analgesic activities than the comparison drug. The saponins demonstrated 7.92% and 14.51% anti-inflammatory activities at the dose of 126 mg/kg and 252 mg/kg, respectively, and 15.38% and 26.15% analgesic activities at the dose of 126 mg/kg and 252 mg/kg, respectively. CONCLUSION: The saponins of D. zingiberensis C. H. Wright possess acute nonspecific anti-inflammatory and analgesic effects. The anti-inflammatory and analgesic effects of ABDE are equivalent to aspirin.

An efficient primary screening of COVID-19 by serum Raman spectroscopy.

The outbreak of COVID-19 coronavirus disease around the end of 2019 has become a pandemic. The preferred method for COVID-19 detection is the real-time polymerase chain reaction (RT-PCR)-based technique; however, it also has certain limitations, such as sample-dependent procedures with a relatively high false negative ratio. We propose a safe and efficient method for screening COVID-19 based on Raman spectroscopy. A total of 177 serum samples are collected from 63 confirmed COVID-19 patients, 59 suspected cases, and 55 healthy individuals as a control group. Raman spectroscopy is adopted to analyze these samples, and a machine learning support-vector machine (SVM) method is applied to the spectrum dataset to build a diagnostic algorithm. Furthermore, 20 independent individuals, including 5 asymptomatic COVID-19 patients and 5 symptomatic COVID-19 patients, 5 suspected patients, and 5 healthy patients, were sampled for external validation. In these three groups-confirmed COVID-19, suspected, and healthy individuals-the distribution of statistically significant points of difference showed highly consistency for intergroups after repeated sampling processes. The classification accuracy between the COVID-19 cases and the suspected cases is 0.87 (95% confidence interval [CI]: 0.85-0.88), and the accuracy between the COVID-19 and the healthy controls is 0.90 (95% CI: 0.89-0.91), while the accuracy between the suspected cases and the healthy control group is 0.68 (95% CI: 0.67-0.73). For the independent test dataset, we apply the obtained SVM model to the classification of the independent test dataset to have all the results correctly classified. Our model showed that the serum-level classification results were all correct for independent test dataset. Our results suggest that Raman spectroscopy could be a safe and efficient technique for COVID-19 screening.

Reservoir CO2 evasion flux and controlling factors of carbon species traced by δ13CDIC at different regulating phases of a hydro-power dam.

As the world largest hydropower reservoir, the Three Gorges Reservoir (TGR) significantly impacted on the carbon cycle since reservoirs are sources of carbon sink. This study was carried out to investigate the effects of damming on the carbon cycle. δ13CDIC and δ13CDOC were used to trace the origin of dissolved organic (DOC) and inorganic carbon (DIC). The estimated CO2 evasion flux in two regulating phases (discharge and recharge) with averages of 111 mg/m2 h and 264 mg/m2 h, respectively. At the basin scale, average CO2 flux was about 188 mg/m2 h and varies from -158 mg/m2 h to 1092 mg/m2 h. The highest average pCO2 (1294 ppmv) was observed during the discharge period, which was oversaturated than atmospheric equilibrium value; hence, the TGR act as a considerable sink of atmospheric carbon. The δ13CDIC varies from -8.95‰ to 0.00‰ with mean -1.87‰; these enrich isotope values indicated that metabolic process (photosynthesis and respiration) and the rapid kinetics of carbonate weathering by soil CO2 control the pCO2. The low pCO2 of reservoir water caused the rapid dissolution of CO2 from the atmosphere during the recharge period. The δ13CDOC varies between -30.64‰ to -23.05‰, which is similar to the values of C3 vegetation; thus, the source of DOC would be the degradation of soil organic matter. Overall, this study revealed the δ13CDIC signature coupled with soil CO2 dissolution and admixture of atmospherically equilibrated waters resulting in the sink of atmospheric CO2 of the reservoir and impoundment of the dam alters the carbon cycle and aquatic carbon budget in TGR. The findings of this study provide a global image on the contribution of reservoirs to the carbon cycle and aquatic carbon budget. Coupling with isotope signatures and elemental concentrations, investigation of the biogeochemical cycle of the carbon can be effectively traced.

Greenhouse gas emissions from riparian zone cropland in a tributary bay of the Three Gorges Reservoir, China.

BACKGROUND: A huge reservoir was formed by the Three Gorges Dam in China, which also formed a riparian zone along the bank of the reservoir. In the period of low water-level, the riparian zone in tributary bays of the Three Gorges Reservoir (TGR) was always unordered cultivated, owing to its gentle slope and high soil fertility. This land-use practice creates high potential of generating greenhouse gas (GHG) emissions with periodic water level fluctuation. METHODS: To evaluate potential GHG emissions from the soil-air interface, the static opaque chamber method was adopted to evaluate the effect of elevations (180 m, 175 m, 170 m and 165 m) and land use types (dry lands, paddy fields and grass fields) from April to September in 2015 and 2016. RESULTS: The results showed that carbon dioxide (CO2) was the main contributor of GHG emission in riparian zone most likely because of high organic carbon from residues. Furthermore, high soil water content in paddy fields resulted in significantly higher methane (CH4) flux than that in dry lands and grass fields. Compared to grass fields, anthropogenic activities in croplands were attributed with a decrease of soil total carbon and GHG emissions. However, inundation duration of different elevations was found to have no significant effect on CH4 and CO2 emissions in the riparian zone, and the mean nitrous oxide (N2O) flux from dry lands at an elevation of 165 m was significantly higher than that of other elevations likely because of tillage and manure application. The high N2O fluxes produced from tillage and fertilizer suggested that, in order to potentially mitigate GHG emissions from the riparian zone, more attention must be paid to the farming practices in dry lands at low elevations (below 165 m) in the riparian zone. Understanding factors that contribute to GHG emissions will help guide ecological restoration of riparian zones in the TGR.