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Plant Cell Environ ; 35(4): 790-805, 2012 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-22026815

RESUMO

Water deficit affects tree growth and limits wood production. In an attempt to identify the molecular triggers of adaptation mechanisms to water deficit in Eucalyptus, we investigated protein expression patterns of two ecophysiologically contrasted Eucalyptus genotypes. They were grown in the field in either natural conditions or irrigated for 7 weeks during the dry season in the Republic of Congo. At the phenotypic level, genotype (G), treatment (T) and/or G × T interaction effects were observed for above- and below-ground biomass-related traits. At the molecular level, changes in protein abundance were recorded in leaves (acidic pH 4-7, and basic pH 7-11, proteomes) and stems (acidic proteome) using two-dimensional gel electrophoresis (2-DE). One third of the detected protein spots displayed significant G, T and/or G × T effects, and 158 of them were identified by tandem mass spectrometry (LC-MS/MS) analysis. Thus, several proteins whose molecular plasticity was genetically controlled (i.e. G × T effect) were revealed, highlighting adaptive mechanisms to water deficit specific to each genotype, namely cell wall modification, cell detoxification and osmoregulation. Transcript abundances corresponding to G × T proteins were also investigated by quantitative RT-PCR. These proteins represent relevant targets to improve drought resistance in this ecologically and economically important forest tree genus.


Assuntos
Adaptação Fisiológica/fisiologia , Eucalyptus/metabolismo , Regulação da Expressão Gênica de Plantas/fisiologia , Proteínas de Plantas/metabolismo , Água/fisiologia , Biomassa , Congo , Secas , Eletroforese em Gel Bidimensional , Eucalyptus/genética , Eucalyptus/fisiologia , Genótipo , Concentração de Íons de Hidrogênio , Fenótipo , Folhas de Planta/genética , Folhas de Planta/metabolismo , Folhas de Planta/fisiologia , Proteínas de Plantas/genética , Caules de Planta/genética , Caules de Planta/metabolismo , Caules de Planta/fisiologia , Proteoma , Proteômica , Estações do Ano , Estresse Fisiológico/fisiologia
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