RESUMO
PURPOSE: It is unclear which triceps tendon repair constructs and techniques produce the strongest biomechanical performance while minimizing the risk of gap formation and repair failure. We aimed to determine associations of construct and technique variables with the biomechanical strength of triceps tendon repairs. PubMed, Embase, Cochrane Library, Web of Science, Scopus, and ClinicalTrials.gov were systematically searched for peer-reviewed studies on biomechanical strength of triceps tendon repairs in human cadavers. 6 articles met the search criteria. Meta-regression was performed on the pooled dataset (123 specimens). Outcomes of interest included gap formation, failure mode, and ultimate failure load. Covariates were fixation type; number of implants; and number of sutures. Stratification by covariates was performed. We found no association between fixation type and ultimate failure load; however, suture anchor fixation was associated with less gap formation compared with transosseous direct repair (ß = - 1.1; 95% confidence interval [CI]:- 2.2, - 0.04). A greater number of implants was associated with smaller gap formation (ß = - 0.77; 95% CI: - 1.3, - 0.28) while a greater number of sutures was associated with higher ultimate failure load ( ß= 3; 95% CI: 21, 125). In human cadaveric models, the number of sutures used in triceps tendon repairs may be more important than the fixation type or number of implants for overall strength. If using a transosseous direct repair approach to repair triceps tendon tears, surgeons may choose to use more sutures in their repair in order to balance the risk of larger gap formation when compared to indirect repair techniques. LEVEL OF EVIDENCE: Level III.
Assuntos
Cadáver , Técnicas de Sutura , Traumatismos dos Tendões , Humanos , Fenômenos Biomecânicos , Âncoras de Sutura , Traumatismos dos Tendões/cirurgia , Tendões/cirurgiaRESUMO
In late summer 1999, an outbreak of human encephalitis occurred in the northeastern United States that was concurrent with extensive mortality in crows (Corvus species) as well as the deaths of several exotic birds at a zoological park in the same area. Complete genome sequencing of a flavivirus isolated from the brain of a dead Chilean flamingo (Phoenicopterus chilensis), together with partial sequence analysis of envelope glycoprotein (E-glycoprotein) genes amplified from several other species including mosquitoes and two fatal human cases, revealed that West Nile (WN) virus circulated in natural transmission cycles and was responsible for the human disease. Antigenic mapping with E-glycoprotein-specific monoclonal antibodies and E-glycoprotein phylogenetic analysis confirmed these viruses as WN. This North American WN virus was most closely related to a WN virus isolated from a dead goose in Israel in 1998.
Assuntos
Surtos de Doenças , Febre do Nilo Ocidental/epidemiologia , Febre do Nilo Ocidental/virologia , Vírus do Nilo Ocidental/classificação , Vírus do Nilo Ocidental/genética , Sequência de Aminoácidos , Animais , Anticorpos Monoclonais/imunologia , Anticorpos Antivirais/imunologia , Sequência de Bases , Doenças das Aves/epidemiologia , Doenças das Aves/virologia , Aves/virologia , Vírus da Encefalite Japonesa (Subgrupo)/classificação , Vírus da Encefalite Japonesa (Subgrupo)/genética , Técnica Indireta de Fluorescência para Anticorpo , Genoma Viral , Humanos , Dados de Sequência Molecular , New England/epidemiologia , Cidade de Nova Iorque/epidemiologia , Filogenia , Aves Canoras/virologia , Proteínas do Envelope Viral/química , Proteínas do Envelope Viral/genética , Proteínas do Envelope Viral/imunologia , Febre do Nilo Ocidental/veterinária , Vírus do Nilo Ocidental/imunologia , Vírus do Nilo Ocidental/isolamento & purificaçãoRESUMO
Approximately 2 billion people live in countries where Japanese encephalitis (JE) presents a significant risk to humans and animals, particularly in China and India, with at least 700 million potentially susceptible children. The combined effects of climate change, altered bird migratory patterns, increasing movement of humans, animals and goods, increasing deforestation and development of irrigation projects will inevitably lead to further geographic dispersal of the virus and an enhanced threat. Although most human infections are mild or asymptomatic, some 50% of patients who develop encephalitis suffer permanent neurologic defects, and 25% die. Vaccines have reduced the incidence of JE in some countries. No specific antiviral therapy is currently available. Interferon alpha-2a was tested in a double-blind placebo-controlled trial on children with Japanese encephalitis, but with negative results. There is thus a real need for antivirals that can reduce the toll of death and neurological sequelae resulting from infection with JE virus. Here we briefly review the epidemiological problems presented by this virus, the present state of drug development and the contributory role that antiviral therapy might play in developing future control strategies for JE.
Assuntos
Antivirais/uso terapêutico , Vírus da Encefalite Japonesa (Espécie)/efeitos dos fármacos , Encefalite Japonesa/epidemiologia , Encefalite Japonesa/prevenção & controle , Idoso , Animais , Criança , Pré-Escolar , Encefalite Japonesa/tratamento farmacológico , Encefalite Japonesa/virologia , HumanosRESUMO
This introduction provides a telegraphic overview of the processes of zoonotic viral emergence, the intricacies of host-virus interactions, and the distinct role of biological transitions and modifying factors. The process of emergence is conceptualized as two transition stages which are common and required for all disease emergence, (1) human contact with the infectious agent and (2) cross-species transmission of the agent, and two transition stages which are not required for emergence and appear unavailable to many zoonotic pathogens, (3) sustained human-to-human transmission and (4) genetic adaptation to the human host. The latter two transitions are presumably prerequisites for the pandemic emergence of a pathogen. The themes introduced herein are amplified and explored in detail by the contributors to this volume. Each author explores the mechanisms and unique circumstances by which evolution, biology, history, and current context have contrived to drive the emergence of different zoonotic agents by a series of related events; although recognizable similarities exist among the events leading to emergence the details and circumstances are never repetitive.
Assuntos
Animais Selvagens/virologia , Doenças Transmissíveis Emergentes/transmissão , Doenças Transmissíveis Emergentes/veterinária , Viroses/transmissão , Viroses/veterinária , Zoonoses , Animais , Doenças Transmissíveis Emergentes/virologia , Humanos , Especificidade da Espécie , Viroses/virologiaRESUMO
Two related, novel, zoonotic paramyxoviruses have been described recently. Hendra virus was first reported in horses and thence humans in Australia in 1994; Nipah virus was first reported in pigs and thence humans in Malaysia in 1998. Human cases of Nipah virus infection, apparently unassociated with infection in livestock, have been reported in Bangladesh since 2001. Species of fruit bats (genus Pteropus) have been identified as natural hosts of both agents. Anthropogenic changes (habitat loss, hunting) that have impacted the population dynamics of Pteropus species across much of their range are hypothesised to have facilitated emergence. Current strategies for the management of henipaviruses are directed at minimising contact with the natural hosts, monitoring identified intermediate hosts, improving biosecurity on farms, and better disease recognition and diagnosis. Investigation of the emergence and ecology of henipaviruses warrants a broad, cross-disciplinary ecosystem health approach that recognises the critical linkages between human activity, ecological change, and livestock and human health.
Assuntos
Quirópteros/virologia , Reservatórios de Doenças/veterinária , Vírus Hendra , Infecções por Henipavirus/veterinária , Vírus Nipah , Animais , Bangladesh/epidemiologia , Surtos de Doenças/veterinária , Reservatórios de Doenças/virologia , Vírus Hendra/classificação , Vírus Hendra/patogenicidade , Infecções por Henipavirus/epidemiologia , Infecções por Henipavirus/transmissão , Humanos , Malásia/epidemiologia , Vírus Nipah/classificação , Vírus Nipah/patogenicidade , Filogenia , Fatores de Risco , ZoonosesRESUMO
Monocyte macrophages (Mphi) are thought to be the principal target cells for the dengue viruses (DV), the cause of dengue fever and hemorrhagic fever. Cell attachment is mediated by the virus envelope (E) protein, but the host-cell receptors remain elusive. Currently, candidate receptor molecules include proteins, Fc receptors, glycosaminoglycans (GAGs) and lipopolysaccharide binding CD14-associated molecules. Here, we show that in addition to Mphi, cells of the T- and B-cell lineages, and including cells lacking GAGs, can bind and become infected with DV. The level of virus binding varied widely between cell lines and, notably, between virus strains within a DV serotype. The latter difference may be ascribable to one or more amino acid differences in domain II of the E protein. Heparin had no significant effect on DV binding, while heparinase treatment of cells in all cases increased DV binding, further supporting the contention that GAGs are not required for DV binding and infection of human cells. In contrast to a recent report, we found that lipopolysaccharide (LPS) had either no effect or enhanced DV binding to, and infection of, various human leukocyte cell lines, while in all virus-cell combinations, depletion of Ca(2+)/Mg(2+) enhanced DV binding. This argues against involvement of beta(2) integrins in virus-host cell interactions, a conclusion in accord with the demonstration of three virus binding membrane proteins of < 75 kDa. Collectively, the results of this study question the purported exclusive importance of the E protein domain III in DV binding to host cells and point to a far more complex interaction between various target cells and, notably, individual DV strains.
Assuntos
Vírus da Dengue/patogenicidade , Leucócitos/virologia , Cálcio/farmacologia , Cátions , Linhagem Celular , Vírus da Dengue/genética , Vírus da Dengue/metabolismo , Heparina/farmacologia , Heparina Liase/farmacologia , Humanos , Leucócitos/metabolismo , Lipopolissacarídeos/farmacologia , Magnésio/farmacologia , Proteínas de Membrana/metabolismo , Receptores Virais/metabolismo , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Homologia de Sequência de Aminoácidos , Proteínas do Envelope Viral/genética , Virulência/efeitos dos fármacos , Replicação ViralRESUMO
The flavivirus non-structural glycoprotein NS1 is often detected in Western blots as a heterogeneous cluster of bands due to glycosylation variations, precursor-product relationships and/or alternative cleavage sites in the viral polyprotein. In this study, we determined the basis of structural heterogeneity of the NS1 protein of Murray Valley encephalitis virus (MVE) by glycosylation analysis, pulse-chase experiments and terminal amino acid sequencing. Inhibition of N-linked glycosylation by tunicamycin revealed that NS1 synthesised in MVE-infected C6/36 cells was derived from two polypeptide backbones of 39 kDa (NS1(o)) and 47 kDa (NS1'). Pulse-chase experiments established that no precursor-product relationship existed between NS1(o) and NS1' and that both were stable end products. Terminal sequencing revealed that the N- and C-termini of NS1(o) were located at amino acid positions 714 and 1145 in the polyprotein respectively, consistent with the predicted sites based upon sequence homology with other flaviviruses. Expression of the NS1 gene alone or in conjunction with NS2A by recombinant baculoviruses demonstrated that the production of NS1' was dependent on the presence of NS2A, indicating that the C-terminus of the larger protein was generated within NS2A. A smaller form (31 kDa) of NS1 (deltaNS1) was also identified in MVE-infected Vero cultures, and amino acid sequencing revealed a 120-residue truncation at the N-terminus of this protein. This corresponds closely with the in-frame 121-codon deletion at the 5' end of the NS1 gene of defective MVE viral RNA (described by Lancaster et al. in 1998), suggesting that deltaNS1 may be a translation product of defective viral RNA.
Assuntos
Vírus da Encefalite do Vale de Murray/metabolismo , Proteínas não Estruturais Virais/metabolismo , Aedes/citologia , Sequência de Aminoácidos , Animais , Baculoviridae , Carboidratos/análise , Linhagem Celular , Chlorocebus aethiops , Expressão Gênica , Vetores Genéticos , Dados de Sequência Molecular , Peptídeos/metabolismo , Proteínas Recombinantes de Fusão/genética , Proteínas Recombinantes de Fusão/metabolismo , Spodoptera/citologia , Células Vero , Proteínas não Estruturais Virais/genéticaRESUMO
We describe herein the molecular epidemiology and phylogeny of Kokobera (KOK) virus, a flavivirus found in Australia and Papua New Guinea. We sequenced a region encompassing the 200 nucleotides of the 3' terminus of the NS5 gene, and the first 300 nucleotides of the 3' untranslated region (UTR). The study included 25 isolates of the virus, including an isolate from PNG, and several recent isolates from the south-west of Western Australia (WA), where the virus had not previously been detected. We found that the KOK isolates clustered according to geographic location and time of isolation into three distinct topotypes: one covering Queensland and New South Wales; another represented by the single isolate from PNG; and a third covering the Northern Territory and WA. This latter group was further subdivided into northern and south-west isolates. This molecular epidemiology is significantly different from other Australian flaviviruses, such as Murray Valley encephalitis (MVE) and Kunjin (KUN) viruses, which exist as single genetic types across the entire Australian continent. However, it is similar to the molecular epidemiology of the alphavirus Ross River (RR) virus. This may be explained by the fact that MVE and KUN viruses are known to have birds as their main vertebrate hosts, whereas RR virus utilises macropods, which have also been implicated as the vertebrate host for KOK virus. In addition, the south-west isolates exhibited a degree of sequence heterogeneity, including one isolate that has a nine nucleotide deletion in the 3'UTR. This suggests that KOK virus has been in the south-west of WA for some time, and was not recently introduced.
Assuntos
Infecções por Flavivirus/epidemiologia , Infecções por Flavivirus/virologia , Flavivirus/genética , Regiões 3' não Traduzidas/genética , Aedes/virologia , Sequência de Aminoácidos , Animais , Culex/virologia , Flavivirus/isolamento & purificação , Epidemiologia Molecular , Dados de Sequência Molecular , Papua Nova Guiné/epidemiologia , Filogenia , Análise de Sequência de DNA , Proteínas não Estruturais Virais/genética , Austrália Ocidental/epidemiologiaRESUMO
Plaques were formed in monolayers of leucocytes prepared from mesenteric lymphnodes (LN) of normal mice. The plaques were first detected after 2 h incubation, followed by a sudden increase in number at 7 h, and reached a peak between 11 and 21 h. Large cells were observed in almost all plaques under the microscope. The large cells attached surrounding leucocytes via cytoplasmic extensions, and held aggregated leucocytes on their cell surface. Aggregated leucocytes were lyzed and/or phagocytized by the large cells. Results suggest that plaque formation was due to the direct, indiscriminate killing of normal leucocytes by the large cells. The large cells increased with age. The large cells were contained in the adherent cell fraction but not the non-adherent fraction, and were removed from leucocytes by the carbonyl iron method. Thus, the large effector cells may be related to macrophages.
Assuntos
Citotoxicidade Imunológica , Linfonodos/imunologia , Linfócitos/imunologia , Mesentério/citologia , Animais , Células Cultivadas , Masculino , Camundongos , Camundongos Endogâmicos , FagocitoseRESUMO
1. The effects of a respiratory tract viral infection on beta-adrenoceptor density, distribution and function were investigated in murine airways. 2. Following intranasal inoculation of CBA/CaH mice with influenza A/PR-8/34 virus, the virus proliferated rapidly in trachea (peak titres 2 days post-inoculation) and lung (peak titres 4-6 days post-inoculation). Respiratory tract viral infection was associated with a significant increase in lung weight (88% higher than control mice at day 6 post-inoculation) that was related temporally to the development of peripheral lung inflammation and consolidation. 3. Analysis of specific binding of [125I]-cyanopindolol to beta-adrenoceptors revealed that on days 2, 4 and 8 post-inoculation with virus, mouse isolated tracheal sections contained, on average, 40% more beta-adrenoceptors than tracheal sections from time matched control mice. Subsequent quantitative autoradiographic studies demonstrated that this increase in total tracheal beta-adrenoceptors was due primarily to a 90% increase in the density of beta-adrenoceptors in the tracheal epithelium in virus-infected mice. 4. In contrast, virus-infection had no significant effect on the density of beta-adrenoceptors in tracheal airway smooth muscle, although within 2 days of inoculation with virus, mouse tracheal smooth muscle segments were approximately 2 fold less sensitive to the beta-adrenoceptor agonist, noradrenaline (mean pD2 = 6.57 +/- 0.04, n = 24) and to the adenylyl cyclase-activator forskolin (mean pD2 = 6.78 +/- 0.04, n = 12) compared to segments from control mice (mean pD2 = 6.84 +/- 0.06 for noradrenaline; mean pD2 = 7.03 +/- 0.07 for forskolin). Similar values were obtained 8 days post-inoculation. At day 2, but not day 8 post-inoculation with virus, relaxation responses to theophylline were also marginally attenuated compared with controls.5. Mouse isolated tracheal segments obtained 2 days after virus inoculation and segments from timematched control mice were equisensitive to the spasmogenic actions of the muscarinic cholinoceptor agonist, carbachol. However, tracheal segments from mice inoculated with virus were less responsive to carbachol on day 4 (mean pD2 = 6.45 + 0.04, n = 8) and day 8 (mean pD2 = 6.45 +/- 0.02, n = 12) compared to control preparations (day 4, mean pD2 = 6.73 +/- 0.06, n = 8; day 8, mean pD2= 6.65 +/- 0.04, n = 12, P < 0.05). In contrast, endothelin-l-induced contractions of tracheal smooth muscle were notaffected by virus-infection.6. These data demonstrate that respiratory tract viral infection can produce significant tissue-selective changes in airway /beta-adrenoceptor density as well as small reductions in airway smooth muscle muscarinic cholinoceptor and /beta-adrenoceptor function.
Assuntos
Vírus da Influenza A , Infecções por Orthomyxoviridae/metabolismo , Receptores Adrenérgicos beta/metabolismo , Infecções Respiratórias/metabolismo , Animais , Autorradiografia , Carbacol/farmacologia , Embrião de Galinha , Técnicas In Vitro , Radioisótopos do Iodo , Iodocianopindolol , Pulmão/efeitos dos fármacos , Pulmão/microbiologia , Masculino , Camundongos , Camundongos Endogâmicos CBA , Contração Muscular/efeitos dos fármacos , Músculo Liso/efeitos dos fármacos , Infecções por Orthomyxoviridae/microbiologia , Pindolol/análogos & derivados , Ensaio Radioligante , Receptores Adrenérgicos beta/efeitos dos fármacos , Traqueia/efeitos dos fármacos , Traqueia/microbiologiaRESUMO
Kunjin (KUN) is a flavivirus in the Japanese encephalitis antigenic complex that was first isolated from Culex annulirostris mosquitoes captured in northern Australia in 1960. It is the etiological agent of a human disease characterized by febrile illness with rash or mild encephalitis and, occasionally, of a neurological disease in horses. KUN virus shares a similar epidemiology and ecology with the closely related Murray Valley encephalitis (MVE) virus, the major causative agent of arboviral encephalitis in Australia. Based on traditional antigenic methods, KUN was initially found to be similar to, but distinct from, reference strains of West Nile (WN) virus and designated as a new species. However, more recent phylogenic analyses have revealed that some strains of WN virus, including the isolates from New York, are more similar to KUN virus and form a separate lineage to other WN viruses. An unusual KUN isolate from Malaysia and the African virus Koutango appear to form additional lineages within the WN group of viruses. While these findings are in agreement with the Seventh Report of the International Committee for the Taxonomy of Viruses that designates KUN as a subtype of West Nile, they also suggest that the species should be further subdivided into additional subtypes.
Assuntos
Febre do Nilo Ocidental/epidemiologia , Vírus do Nilo Ocidental/genética , Animais , Austrália/epidemiologia , Culex , Variação Genética , Humanos , Filogenia , Vírus do Nilo Ocidental/classificaçãoRESUMO
Three newly recognized encephalitogenic zoonotic viruses spread from fruit bats of the genus Pteropus (order Chiroptera, suborder Megachiroptera) have been recognised over the past decade. These are: Hendra virus, formerly named equine morbillivirus, which was responsible for an outbreak of disease in horses and humans in Brisbane, Australia, in 1994; Australian bat lyssavirus, the cause of a severe acute encephalitis, in 1996; and Nipah virus, the cause of a major outbreak of encephalitis and pulmonary disease in domestic pigs and people in peninsula Malaysia in 1999. Hendra and Nipah viruses have been shown to be the first two members of a new genus, Henipavirus, in the family Paramyxoviridae, subfamily Paramyxovirinae, whereas Australian bat lyssavirus is closely related antigenically to classical rabies virus in the genus Lyssavirus, family Rhabdoviridae, although it can be distinguished on genetic grounds. Hendra and Nipah viruses have neurological and pneumonic tropisms. The first humans and equids with Hendra virus infections died from acute respiratory disease, whereas the second human patient died from an encephalitis. With Nipah virus, the predominant clinical syndrome in humans was encephalitic rather than respiratory, whereas in pigs, the infection was characterised by acute fever with respiratory involvement with or without neurological signs. Two human infections with Australian bat lyssavirus have been reported, the clinical signs of which were consistent with classical rabies infection and included a diffuse, non-suppurative encephalitis. Many important questions remain to be answered regarding modes of transmission, pathogenesis, and geographic range of these viruses.
Assuntos
Quirópteros/virologia , Infecções por Henipavirus/transmissão , Henipavirus/patogenicidade , Lyssavirus/patogenicidade , Infecções por Rhabdoviridae/transmissão , Animais , Surtos de Doenças , Geografia , Henipavirus/isolamento & purificação , Infecções por Henipavirus/epidemiologia , Infecções por Henipavirus/veterinária , Doenças dos Cavalos/epidemiologia , Doenças dos Cavalos/transmissão , Cavalos , Humanos , Lyssavirus/isolamento & purificação , Infecções por Rhabdoviridae/epidemiologia , Infecções por Rhabdoviridae/veterinária , ZoonosesRESUMO
OBJECTIVE: To report the cortisol levels in 6 patients during and after severe inflammation. DESIGN: Patients with severe inflammatory disease had basal and stimulated (cosyntropin) serum cortisol levels determined at the time of severe and less severe inflammation. SETTING: Intensive care unit and wards of a tertiary care center. PATIENTS: Six patients with continued evidence of severe inflammation, despite aggressive management of the underlying inflammatory disease. INTERVENTIONS: Five of 6 patients received hydrocortisone at "physiologic" doses. MAIN OUTCOME MEASURES: Basal and stimulated serum cortisol levels. RESULTS: The mean+/-SD cortisol data for these patients were as follows: baseline cortisol level during inflammation, 350+/-121 nmol/L (n=6); stimulated cortisol level during inflammation, 571+/-326 nmol/L (n=6); baseline cortisol level with less inflammation, 833+/-339 nmol/L (P=.03 vs baseline level during inflammation) (n=5); and stimulated cortisol level with less inflammation, 1090+/-295 nmol/L (P=.03 vs stimulated level during inflammation) (n=4). Manifestations of inflammation decreased with hydrocortisone administration. CONCLUSIONS: Severe inflammation may result in lower-than-expected serum cortisol levels, which then increase significantly as the inflammation decreases. Transient hypoadrenalism may aggravate the adverse effects of severe inflammation. These effects may be ameliorated by administering physiologic rather than pharmacologic doses of hydrocortisone.
Assuntos
Insuficiência Adrenal/etiologia , Estado Terminal , Hidrocortisona/sangue , Insuficiência Adrenal/sangue , Adulto , Humanos , Inflamação/sangue , Inflamação/complicações , Masculino , Pessoa de Meia-IdadeRESUMO
Entomologic and virologic factors were investigated to determine whether gray-headed flying foxes (Pteropus poliocephalus) from Indooroopilly Island, Brisbane, Australia could be vertebrate hosts of Ross River (RR) virus. Aedes funereus was the most abundant mosquito species with 6,300-38,700 females per light trap night in the flying fox camp containing gray-headed, black (P. alecto), and little red (P. scapulatus) flying foxes. Sixteen Ae. funereus blood meals from this collection were analyzed by hemoglobin electrophoresis and were found to be from P. alecto. From pledget feeding with RR virus, the infectious dose required to infect 50% of wild caught Ae. funereus was log10 4.2 50% tissue culture infectious doses per mosquito, with a transmission rate to mice of 17% at 9-10 days post infection. Experimental infection of 10 juvenile P. poliocephalus produced viremias of low titer in five animals, with a duration of 1-4 days and a mean of two days. Three percent of colonized Ae. vigilax that fed on the 10 animals during this period became infected. One of the five viremic flying foxes and two of the five aviremic animals produced a detectable immune response by either neutralization or hemagglutination-inhibition tests. Based on the low to moderate vector competence of Ae. funereus for RR virus, and evidence that P. poliocephalus is a poor vertebrate host of RR virus, it is unlikely that RR virus transmission would be maintained between these two species, but it could be maintained by other more competent vector/host pairs.
Assuntos
Aedes/virologia , Infecções por Alphavirus/transmissão , Quirópteros/virologia , Culex/virologia , Insetos Vetores/virologia , Ross River virus/isolamento & purificação , Aedes/fisiologia , Infecções por Alphavirus/imunologia , Animais , Austrália , Quirópteros/imunologia , Culex/fisiologia , Ecologia , Comportamento Alimentar , Densidade Demográfica , Ross River virus/imunologiaRESUMO
Outbreaks of mosquito-borne Ross River (RR) virus disease (epidemic polyarthritis) occur suddenly in the arid north and interior of the State of Western Australia, often within a few weeks of heavy rainfall. Between outbreaks, these regions may undergo long periods of drought, with little or no mosquito or arbovirus activity. The means by which RR virus is reintroduced or reactivated in these areas when environmental conditions favor mosquito-borne virus activity are unknown. In this paper, we describe isolations of RR virus from eight mosquito species trapped at two different locations, one coastal and one inland, in the arid Pilbara region of Western Australia, prior to outbreaks of epidemic polyarthritis. The isolation of RR virus has not been previously reported for five of these species and the isolations from the other three species are new records for Western Australia. The timing and number of isolations of RR virus from Aedes (Ochlerotatus) vigilax (Skuse, 1889) implicate that species as a vector of the virus on the Pilbara coast. Significantly, RR virus was isolated from pools of male Ae. vigilax and male Ae. (Macleaya) tremulus (Theobald, 1903) mosquitoes. This is the first report of RR virus (or other Australian arbovirus) isolates from wild-caught male mosquitoes. Both Ae. vigilax and Ae. tremulus have desiccation-resistant eggs that can survive long periods of drought, making them ideal candidates for the overwintering of arboviruses. The findings implicate vertical transmission as a means of persistence of RR virus in arid regions of Australia and therefore offer a likely explanation for the sudden recurrence of virus activity after heavy rains.
Assuntos
Aedes/microbiologia , Infecções por Alphavirus/epidemiologia , Artrite Infecciosa/epidemiologia , Insetos Vetores/microbiologia , Ross River virus/isolamento & purificação , Animais , Feminino , Humanos , Masculino , Chuva , Austrália Ocidental/epidemiologiaRESUMO
Two possible mechanisms are described for the initiation of Murray Valley encephalitis (MVE) virus activity in arid, epizootic regions of tropical Australia. Virus isolations were made from mosquitoes trapped shortly after the first heavy wet season rains and flooding in the east Kimberley, which followed approximately nine months of drought. A number of isolates of MVE virus were obtained, including isolates from pools of blood-engorged Culex annulirostris mosquitoes and from a single pool of male Aedes tremulus mosquitoes. The results strongly suggested that MVE virus activity was due both to its introduction in viremic vertebrate hosts, from which first-generation mosquitoes became infected following blood meals, and also to reactivation of vertically transmitted virus from desiccation-resistant eggs of Ae. tremulus. Both mechanisms are discussed with respect to environmental conditions.
Assuntos
Aedes/virologia , Culex/virologia , Vírus da Encefalite do Vale de Murray/fisiologia , Insetos Vetores/virologia , Ativação Viral/fisiologia , Animais , Vírus da Encefalite do Vale de Murray/isolamento & purificação , Encefalite por Arbovirus/epidemiologia , Encefalite por Arbovirus/transmissão , Feminino , Humanos , Transmissão Vertical de Doenças Infecciosas , Masculino , Chuva , Cultura de Vírus , Austrália Ocidental/epidemiologiaRESUMO
The flavivirus Japanese encephalitis (JE) virus has recently emerged in the Australasian region. To investigate the involvement of infections with related enzootic flaviviruses, namely Murray Valley encephalitis (MVE) virus and Kunjin (KUN) virus, on immunity of pigs to JE virus and to provide a basis for interpretation of serologic data, experimental infections were conducted with combinations of these viruses. Antibody responses to primary and secondary infections were evaluated using panels of monoclonal antibody-based blocking enzyme-linked immunosorbent assays and microtiter serum neutralization tests (mSNTs). Identification of the primary infecting virus was possible only using the mSNTs. Following challenge, unequivocal diagnosis was impossible due to variation in immune responses between animals and broadened and/or anamnestic responses. Viremia for JE virus was readily detected in pigs following primary infection, but was not detected following prior exposure to MVE or KUN viruses. Boosted levels of existing cross-neutralizing antibodies to JE virus suggested a role for this response in suppressing JE viremia.
Assuntos
Anticorpos Antivirais/biossíntese , Vírus da Encefalite Japonesa (Espécie)/imunologia , Vírus da Encefalite do Vale de Murray/imunologia , Encefalite Japonesa/prevenção & controle , Vírus do Nilo Ocidental/imunologia , Animais , Anticorpos Monoclonais/imunologia , Anticorpos Antivirais/sangue , Estudos de Casos e Controles , Modelos Animais de Doenças , Ensaio de Imunoadsorção Enzimática , Testes de Neutralização , Suínos , Viremia/diagnósticoRESUMO
In response to an outbreak of Japanese encephalitis (JE) virus on Cape York Peninsula, Australia, in 1998, mosquitoes were collected using CO2 and octenol-baited Centers for Disease Control and Prevention light traps. A total of 35,235 adult mosquitoes, comprising 31 species, were processed for virus isolation. No isolates of JE virus were recovered from these mosquitoes. However, 18 isolates of Kokobera virus, another flavivirus were obtained from Culex annulirostris. Twelve isolates were from western Cape York (minimum infection rate (MIR) of 0.61: 1,000 mosquitoes) and 6 were from the Northern Peninsula Area (MIR of 1.0:1,000). Potential explanations for the failure to detect JE virus in mosquitoes collected from Cape York Peninsula include the timing of collections, the presence of alternative bloodmeal hosts, differences in pig husbandry, asynchronous porcine seroconversion, and the presence of other flaviviruses.
Assuntos
Culicidae/virologia , Surtos de Doenças , Encefalite Japonesa/epidemiologia , Flavivirus/isolamento & purificação , Insetos Vetores/virologia , Animais , Culicidae/classificação , DNA Viral/isolamento & purificação , Encefalite Japonesa/prevenção & controle , Humanos , Insetos Vetores/classificação , Queensland/epidemiologia , Reação em Cadeia da Polimerase Via Transcriptase ReversaRESUMO
An increase in the incidence of polyarthritis caused by Barmah Forest (BF) virus, and its recent emergence into Western Australia, prompted a study of the molecular epidemiology of this Australian mosquito-borne alphavirus. The nucleotide sequence of a 500-basepair region of the 3' end of the envelope (E2) gene of the prototype BF virus strain (BH2193) was compared with other members of the alphavirus genus, and to a panel of isolates of BF virus collected more for than 20 years from different geographic regions of Australia. The BF virus was shown to be genetically distinct from other members of the alphavirus genus. A high degree of sequence homology (98-100%) was found between the BF isolates, with no evidence of geographic or temporal divergence. This nucleotide homogeneity was similar to that observed with other Australian mosquito-borne viruses with avian vertebrate hosts, such as Sindbis, Murray Valley, and Kunjin viruses, but it contrasts to the heterogeneity reported for Ross River virus, an alphavirus with mammalian vertebrate hosts.
Assuntos
Infecções por Alphavirus/epidemiologia , Alphavirus/genética , Artrite Infecciosa/virologia , Epidemiologia Molecular , Proteínas do Envelope Viral/genética , Sequência de Aminoácidos , Animais , Artrite Infecciosa/epidemiologia , Austrália/epidemiologia , Células Cultivadas , Chlorocebus aethiops , Biblioteca Gênica , Humanos , Dados de Sequência Molecular , Filogenia , Reação em Cadeia da Polimerase , Alinhamento de Sequência , Análise de Sequência de DNA , Homologia de Sequência de Aminoácidos , Células VeroRESUMO
After Japanese encephalitis (JE) virus emerged in the Torres Strait in Australia in 1995, investigations were initiated into the origin of the incursion. New Guinea was considered the most likely source, given its proximity to islands of the Torres Strait. Almost 400,000 adult mosquitoes were processed for virus isolation from 26 locations in the Western Province of Papua New Guinea (PNG) between February 1996 and February 1998, yielding three isolates of JE virus. Two isolates of Murray Valley encephalitis, 17 isolates of Sindbis, and 1 each of Sepik and Ross River viruses were also obtained. Nucleic acid sequences of the PNG JE isolates were determined in the prM region, and in a region overlapping a part of the fifth nonstructural protein and the 3' untranslated region. The PNG isolates belonged to genotype II, and shared > 99.2% identity with isolates from humans and mosquitoes from the Torres Strait, suggesting that PNG is the source of incursions of JE virus into Australia.