Continuously Sheared Granular Matter Reproduces in Detail Seismicity Laws.

We introduce a shear experiment that quantitatively reproduces the main laws of seismicity. By continuously and slowly shearing a compressed monolayer of disks in a ringlike geometry, our system delivers events of frictional failures with energies following a Gutenberg-Richter law. Moreover, foreshocks and aftershocks are described by Omori laws and interevent times also follow exactly the same distribution as real earthquakes, showing the existence of memory of past events. Other features of real earthquakes qualitatively reproduced in our system are both the existence of a quiescence preceding some main shocks, as well as magnitude correlations linked to large quakes. The key ingredient of the dynamics is the nature of the force network, governing the distribution of frictional thresholds.

Particle-covered drops in electric fields: drop deformation and surface particle organization.

Drops covered by adsorbed particles are a prominent research topic because they hold promise for a variety of practical applications. Unlocking the enormous potential of particle-laden drops in new material fabrication, for instance, requires understanding how surface particles affect the electrical and deformation properties of drops, as well as developing new routes for particle manipulation at the interface of drops. In this study, we utilized electric fields to experimentally investigate the mechanics of particle-covered silicone oil drops suspended in castor oil, as well as particle assembly at drop surfaces. We used particles with electrical conductivities ranging from insulating polystyrene to highly conductive silver. When subjected to electric fields, drops can change shape, rotate, or break apart. In the first part of this work, we demonstrate how the deformation magnitude and shape of drops, as well as their electrical properties, are affected by electric field strength, particle size, conductivity, and coverage. We also discuss the role of electrohydrodynamic flows on drop deformation. In the second part, we present the electric field-directed assembly and organization of particles at drop surfaces. In this regard, we studied various parameters in detail, including electric field strength, particle size, coverage, and electrical conductivity. Finally, we present a novel method for controlling the local particle coverage and packing of particles on drop surfaces by simply tuning the frequency of the applied electric field. This approach is expected to find uses in optical materials and applications.

Thermally activated crack fronts propagating in pinning disorder: simultaneous brittle/creep behaviour depending on scale.

We study theoretically the propagation of a crack front in mode I along an interface in a disordered elastic medium, with a numerical model considering a thermally activated rheology, toughness disorder and long-range elastic interactions. This model reproduces not only the large-scale dynamics of the crack front position in fast or creep loading regimes, but also the small-scale self-affine behaviour of the front. Two different scaling laws are predicted for the front morphology, with a Hurst exponent of 0.5 at small scales and a logarithmic scaling law at large scales, consistently with experiments. The prefactor of these scaling laws is expressed as a function of the temperature, and of the quenched disorder characteristics. The cross-over between these regimes is expressed as a function of the quenched disorder amplitude, and is proportional to the average energy release rate, and to the inverse of temperature. This model captures as well the experimentally observed local velocity fluctuation probability distribution, with a high-velocity tail P(v)∼v -2.6 This feature is shown to arise when the quenched disorder is sufficiently large, whereas smaller toughness fluctuations lead to a lognormal-like velocity distribution. Overall, the system is shown to obey a scaling determined by two distinct mechanisms as a function of scale: namely, the large scales display fluctuations similar to an elastic line in an annealed noise excited as the average front travels through the pinning landscape, while small scales display a balance between thresholds in possible elastic forces and quenched disorder.This article is part of the theme issue 'Statistical physics of fracture and earthquakes'.

Avalanches and extreme value statistics in interfacial crackling dynamics.

We study the avalanche and extreme statistics of the global velocity of a crack front, propagating slowly along a weak heterogeneous interface of a transparent polymethyl methacrylate block. The different loading conditions used (imposed constant velocity or creep relaxation) lead to a broad range of average crack front velocities. Our high-resolution and large dataset allows one to characterize in detail the observed intermittent crackling dynamics. We specifically measure the size S, the duration D, as well as the maximum amplitude [Formula: see text] of the global avalanches, defined as bursts in the interfacial crack global velocity time series. Those quantities characterizing the crackling dynamics follow robust power-law distributions, with scaling exponents in agreement with the values predicted and obtained in numerical simulations of the critical depinning of a long-range elastic string, slowly driven in a random medium. Nevertheless, our experimental results also set the limit of such model which cannot reproduce the power-law distribution of the maximum amplitudes of avalanches of a given duration reminiscent of the underlying fat-tail statistics of the local crack front velocities.This article is part of the theme issue 'Statistical physics of fracture and earthquakes'.

MHC class II antigen presentation by intestinal epithelial cells fine-tunes bacteria-reactive CD4 T cell responses.

Although intestinal epithelial cells (IECs) can express major histocompatibility complex class II (MHC II), especially during intestinal inflammation, it remains unclear if antigen presentation by IECs favours pro- or anti-inflammatory CD4+ T cell responses. Using selective gene ablation of MHC II in IECs and IEC organoid cultures, we assessed the impact of MHC II expression by IECs on CD4+ T cell responses and disease outcomes in response to enteric bacterial pathogens. We found that intestinal bacterial infections elicit inflammatory cues that greatly increase expression of MHC II processing and presentation molecules in colonic IECs. Whilst IEC MHC II expression had little impact on disease severity following Citrobacter rodentium or Helicobacter hepaticus infection, using a colonic IEC organoid-CD4+ T cell co-culture system, we demonstrate that IECs can activate antigen-specific CD4+ T cells in an MHC II-dependent manner, modulating both regulatory and effector Th cell subsets. Furthermore, we assessed adoptively transferred H. hepaticus-specific CD4+ T cells during intestinal inflammation in vivo and report that IEC MHC II expression dampens pro-inflammatory effector Th cells. Our findings indicate that IECs can function as non-conventional antigen presenting cells and that IEC MHC II expression fine-tunes local effector CD4+ T cell responses during intestinal inflammation.

Magnetic Janssen effect.

A pile of grains, even when at rest in a silo, can display fascinating properties. One of the most celebrated is the Janssen effect, named after the pioneering engineer who explained the pressure saturation at the bottom of a container filled with corn. This surprising behavior arises because of frictional interactions between the grains through a disordered network of contacts, and the vessel lateral walls, which partially support the weight of the column, decreasing its apparent mass. Here, we demonstrate control over frictional interactions using ferromagnetic grains and an external magnetic field. We show that the anisotropic pairwise interactions between magnetized grains result in a radial force along the walls, whose amplitude and direction is fully determined by the applied magnetic field. Such magnetic Janssen effect allows for the fine tuning of the granular column apparent mass. Our findings pave the way towards the design of functional jammed materials in confined geometries, via a further control of both their static and dynamic properties.

CD4(+) T cell subsets during virus infection. Protective capacity depends on effector cytokine secretion and on migratory capability.

To analyze the antiviral protective capacities of CD4(+) T helper (Th) cell subsets, we used transgenic T cells expressing an I-A(b)-restricted T cell receptor specific for an epitope of vesicular stomatitis virus glycoprotein (VSV-G). After polarization into Th1 or Th2 effectors and adoptive transfer into T cell-deficient recipients, protective capacities were assessed after infection with different types of viruses expressing the VSV-G. Both Th1 and Th2 CD4(+) T cells could transfer protection against systemic VSV infection, by stimulating the production of neutralizing immunoglobulin G antibodies. However, only Th1 CD4(+) T cells were able to mediate protection against infection with recombinant vaccinia virus expressing the VSV-G (Vacc-IND-G). Similarly, only Th1 CD4(+) T cells were able to rapidly eradicate Vacc-IND-G from peripheral organs, to mediate delayed-type hypersensitivity responses against VSV-G and to protect against lethal intranasal infection with VSV. Protective capacity correlated with the ability of Th1 CD4(+) T cells to rapidly migrate to peripheral inflammatory sites in vivo and to respond to inflammatory chemokines that were induced after virus infection of peripheral tissues. Therefore, the antiviral protective capacity of a given CD4(+) T cell is governed by the effector cytokines it produces and by its migratory capability.

Coupled air/granular flow in a linear Hele-Shaw cell.

We investigate experimentally the pattern formation process during injection of air in a noncohesive granular material confined in a linear Hele-Shaw cell. We characterize the features and dynamics of this pattern formation on the basis of fast image analysis and sensitive pressure measurements. Behaviors are classified using two parameters--injection pressure and plate opening--and four hydrodynamic regimes are defined. For some regions of the parameter space, flows of air and grains are shown to be strongly coupled and instable, and lead to channelization within the granular material with obvious large-scale permeability variations.

Revolving rivers in sandpiles: from continuous to intermittent flows.

In a previous paper [E. Altshuler, Phys. Rev. Lett. 91, 014501 (2003)], the mechanism of "revolving rivers" for sandpile formation is reported: As a steady stream of dry sand is poured onto a horizontal surface, a pile forms which has a river of sand on one side flowing from the apex of the pile to the edge of the base. For small piles the river is steady, or continuous. For larger piles, it becomes intermittent. In this paper we establish experimentally the "dynamical phase diagram" of the continuous and intermittent regimes, and give further details of the piles' "topography," improving the previous kinematic model to describe it and shedding further light on the mechanisms of river formation. Based on experiments in Hele-Shaw cells, we also propose that a simple dimensionality reduction argument can explain the transition between the continuous and intermittent dynamics.

Decompaction and fluidization of a saturated and confined granular medium by injection of a viscous liquid or gas.

We compare quantitatively two experimental situations concerning injection of a miscible fluid into an initially jammed granular medium saturated with the same fluid, confined in a Hele-Shaw cell. The two experiments are identical, apart from the interstitial and injected fluid, which is in one case air injected into a dry granular packing, and in the other case silicone oil injected into a dense suspension. In spite of the strong differences regarding the nature of the two fluids, strikingly similar dynamical and geometrical features are identified as functions of the control parameters: cell thickness and applied fluid injection pressure. In both cases an initial hydrodynamically driven decompaction process controls the unjamming and prepares the final displacement process characterized by fingerlike patterns empty of grains. The pattern shapes are comparable. In addition, the mobilities of the coupled fluid-grain flow, rescaled by the interstitial fluid viscosity and grain diameter squared, are of the same range and behave comparably. The mobility proves to depend on the initial solid fraction of the medium. Subtle differences are observed in geometrical aspects like the finger width with respect to the control parameters.

Uphill solitary waves in granular flows.

We have experimentally observed uphill solitary waves in the surface flow on a granular material. A heap is constructed by injecting sand between two vertical glass plates separated by a distance much larger than the average grain size, with an open boundary. As the heap reaches the open boundary, solitary fluctuations appear on the flowing layer and move "up the hill" (i.e., against the direction of the flow). We explain the phenomenon in the context of stop-and-go traffic models.

Pattern formation during air injection into granular materials confined in a circular Hele-Shaw cell.

We investigate the dynamics of granular materials confined in a radial Hele-Shaw cell, during central air injection. The behavior of this granular system, driven by its interstitial fluid, is studied both experimentally and numerically. This allows us to explore the associated pattern formation process, characterize its features and dynamics. We classify different hydrodynamic regimes as function of the injection pressure. The numerical model takes into account the interactions between the granular material and the interstitial fluid, as well as the solid-solid interactions between the grains and the confining plates. Numerical and experimental results are comparable, both to reproduce the hydrodynamical regimes experimentally observed, as well as the dynamical features associated to fingering and compacting.

Epithelial-derived IL-18 regulates Th17 cell differentiation and Foxp3⁺ Treg cell function in the intestine.

Elevated levels of interleukin-18 (IL-18) are found in many chronic inflammatory disorders, including inflammatory bowel disease (IBD), and polymorphisms in the IL18R1-IL18RAP locus are associated with IBD susceptibility. IL-18 is an IL-1 family cytokine that has been proposed to promote barrier function in the intestine, but the effects of IL-18 on intestinal CD4(+) T cells are poorly understood. Here we demonstrate that IL-18R1 expression is enhanced on both effector and regulatory CD4(+) T cells in the intestinal lamina propria, with T helper type 17 (Th17) cells exhibiting particularly high levels. We further show that, during steady state, intestinal epithelial cells constitutively secrete IL-18 that acts directly on IL-18R1-expressing CD4(+) T cells to limit colonic Th17 cell differentiation, in part by antagonizing IL-1R1 signaling. In addition, although IL-18R1 is not required for colonic Foxp3(+) regulatory T (Treg) cell differentiation, we found that IL-18R1 signaling was critical for Foxp3(+) Treg cell-mediated control of intestinal inflammation, where it promoted the expression of key Treg effector molecules. Thus IL-18 is a key epithelial-derived cytokine that differentially regulates distinct subsets of intestinal CD4(+) T cells during both homeostatic and inflammatory conditions, a finding with potential implications for treatment of chronic inflammatory disorders.

Bubble propagation in a pipe filled with sand.

Granular flow with strong hydrodynamic interactions has been studied experimentally. Experiments have been carried out to study the movement of a single bubble in an inclined tube filled with glass beads and air. A maximum bubble velocity was found at an inclined angle straight theta(m). The density variations in the sand were measured by capacitance measurements, and a decompactification zone was observed just above the bubble when the inclination angle straight theta was larger than straight theta(m). The length of the decompactification front increased with increasing inclination angle and disappeared for angles smaller than straight theta(m). Both pressure and visualization experiments were carried out and compared with the density measurements.

Synchrotron x-ray scattering studies of water intercalation in a layered synthetic silicate.

Synchrotron x-ray diffraction studies were performed on a synthetic layered silicate Fluorohectorite clay. Diffraction patterns along the stacking direction were obtained in surface reflection and bulk transmission geometries on bulk pressed samples under controlled temperature and relative humidity. One-dimensional structure factors modeling the positions of the intercalant atoms have been obtained for three stable hydration states. From the narrow (00l) peak widths we conclude that well-crystallized domains consist of stacks of about 100 platelets, forming crystallites of the order of 0.1 microm thick. These crystallites have an orientational angular distribution of about 24 degrees around the stacking direction and represent the solid framework for microporosity in these samples.

NLRC4 expression in intestinal epithelial cells mediates protection against an enteric pathogen.

The inflammasomes have an important role in connecting the detection of endogenous and microbial danger signals to caspase-1 activation and induction of protective immune responses. NLRC4 is a cytosolic NOD (nucleotide binding and oligomerization domain)-like receptor (NLR) that can trigger inflammasome formation in response to bacterial flagellin, an immunodominant antigen in the intestine. To characterize the role of NLRC4 in bacterially triggered intestinal inflammation, we used the murine pathogen Citrobacter rodentium, an extracellular, attaching/effacing bacterium similar to enterohemorrhagic Escherichia coli and enteropathogenic E. coli. Following infection with C. rodentium, we found that Nlrc4(-/-) mice developed more severe weight loss, increased bacterial colonization levels, and exacerbated intestinal inflammation compared with wild-type counterparts. Nlrc4(-/-) mice mounted robust adaptive immune responses but were unable to control early colonization by C. rodentium, suggesting that a defect in innate immunity was responsible. Experiments using bone marrow (BM) chimeras revealed that the protective effects of NLRC4 were dependent on its expression in non-hematopoietic cells, and quantitative PCR (Q-PCR) analyses revealed that NLRC4 was highly expressed in epithelial crypts but not in intestinal stroma. Thus, early NLRC4 sensing in intestinal epithelial cells regulates colonization by an extracellular bacterial pathogen and limits subsequent intestinal damage.

Patterns and flow in frictional fluid dynamics.

Pattern-forming processes in simple fluids and suspensions have been studied extensively, and the basic displacement structures, similar to viscous fingers and fractals in capillary dominated flows, have been identified. However, the fundamental displacement morphologies in frictional fluids and granular mixtures have not been mapped out. Here we consider Coulomb friction and compressibility in the fluid dynamics, and discover surprising responses including highly intermittent flow and a transition to quasi-continuous dynamics. Moreover, by varying the injection rate over several orders of magnitude, we characterize new dynamic modes ranging from stick-slip bubbles at low rate to destabilized viscous fingers at high rate. We classify the fluid dynamics into frictional and viscous regimes, and present a unified description of emerging morphologies in granular mixtures in the form of extended phase diagrams.

Avalanche prediction in a self-organized pile of beads.

It is a common belief that power-law distributed avalanches are inherently unpredictable. This idea affects phenomena as diverse as evolution, earthquakes, superconducting vortices, stock markets, etc., from atomic to social scales. It mainly comes from the concept of "self-organized criticality" (SOC), where criticality is interpreted in the way that, at any moment, any small avalanche can eventually cascade into a large event. Nevertheless, this work demonstrates experimentally the possibility of avalanche prediction in the classical paradigm of SOC: a pile of grains. By knowing the position of every grain in a two-dimensional pile, avalanches of moving grains follow a distinct power-law distribution. Large avalanches, although uncorrelated, are on average preceded by continuous, detectable variations in the internal structure of the pile that are monitored in order to achieve prediction.