Transverse relaxometry with reduced echo train lengths via stimulated echo compensation.

Transverse relaxation (T2 ) mapping has many applications, including imaging of iron accumulation in grey matter. Using the typical multiecho spin-echo sequence with long echo trains, stimulated echo compensation can enable T2 fitting under conditions of variable radio frequency homogeneity arising from slice profile and in-plane radio frequency variation. Substantial reduction in the number of refocusing pulses could enable use at high magnetic fields where specific absorption rate is a major limitation, and enable multislice use with reduced incidental magnetization transfer at all field strengths. We examine the effect of reduced echo train lengths and multislice imaging on T2 fitting using stimulated echo compensation applied to iron-rich subcortical grey matter in human brain at 4.7 T. Our findings indicate that reducing from 20 echoes to as few as four echoes can maintain consistent T2 values when using stimulated echo compensation in grey and white matter, but not for cerebrospinal fluid. All territories produce marginal results when using standard exponential fitting. Savings from reduced echoes can be used to substantially increase slice coverage. In multislice mode, the resulting incidental magnetization transfer decreased brain signal but had minimal effect on measured T2 values.

The Fabrication, Implantation, and Stability of Intraspinal Microwire Arrays in the Spinal Cord of Cat and Rat.

Intraspinal microstimulation (ISMS) is currently under investigation for its ability to restore function following spinal cord injury and aid in addressing basic investigations of the spinal cord in feline and murine (rat) models. In this report we describe the procedures for fabricating and implanting intraspinal microwires, with special emphasis on the rat model. We also report our results on targeting success and long-term stability and functionality of the implants. Early targeting with implants fabricated based on general "average" dimensions of the spinal cord was approximately 50% successful in reaching the proper targets within the ventral grey matter in cats. Improvements in insertion technique and the use of multiple contact electrodes have raised the targeting success to 100%. Furthermore, the manufacturing of ISMS arrays has been improved by the use of magnetic resonance imaging to create subject-specific implants for cats and track the location of the arrays post-implant. In the rat, our procedures have produced desirable targeting of all recovered microwires. We speculate this is due to the different targeting parameters and the shorter depth of insertion in the rat spinal cord. Although there is a heightened mechanical mismatch between the 30 µm -diameter microwires and the small rat spinal cord, chronic implantation and stimulation produce limited histological damage and do not compromise function. Furthermore, despite the increased difficulties of implanting into the smaller rat spinal cord, ISMS is effective in activating spinal cord networks in the lumbosacral enlargement in a manner that is safe, stable and reproducible.