RESUMO
We present here the frequency of enteropathogens in an HIV-infected children group and investigate their correlation with clinical and sociodemographic characteristics by collecting 100 stool samples from 55 HIV-seropositive Brazilian children. All specimens were processed according to standard methods for bacterial and yeast detection. A commercially available enzyme-linked immunosorbent assay was used to detect protozoan, and to perform virus detection, molecular tests were applied. Consumption of raw vegetables and fruits and severe immunosuppression were significantly associated with diarrhea. Cryptosporidium parvum was the commonest enteropathogen, followed by Candida albicans, enteropathogenic Escherichia coli, and astrovirus. The number of potential pathogenic agents identified in fecal specimens in asymptomatic HIV-seropositive infants is high, which raises the need for additional investigation in this area as well as in other Brazilian regions.
Assuntos
Infecções Oportunistas Relacionadas com a AIDS , Diarreia , Infecções por HIV/complicações , Infecções Oportunistas Relacionadas com a AIDS/epidemiologia , Infecções Oportunistas Relacionadas com a AIDS/microbiologia , Infecções Oportunistas Relacionadas com a AIDS/parasitologia , Infecções Oportunistas Relacionadas com a AIDS/virologia , Adolescente , Animais , Brasil/epidemiologia , Candida albicans/isolamento & purificação , Criança , Pré-Escolar , Cryptosporidium parvum/isolamento & purificação , Diarreia/epidemiologia , Diarreia/microbiologia , Diarreia/parasitologia , Diarreia/virologia , Escherichia coli/isolamento & purificação , Fezes/microbiologia , Fezes/parasitologia , Fezes/virologia , Feminino , Infecções por HIV/epidemiologia , Infecções por HIV/virologia , HIV-1 , Humanos , Lactente , Masculino , Mamastrovirus/isolamento & purificaçãoRESUMO
We evaluated the diagnostic performance of a Cryptosporidium immunoenzymatic assay (ELISA). Fecal samples were collected from 94 HIV-seropositive patients. All specimens were processed with a commercially-available ELISA to detect C. parvum specific coproantigen and with a modified Ziehl-Neelsen stain (ZNm) microscope exam. Overall, sensitivity of the immunoenzymatic test was 100%, with a specificity of 96%; positive and negative predictive values were 89% and 100%, respectively. The commercial ELISA and ZNm proved to be valuable diagnostic tools for Cryptosporidium infection.
Assuntos
Infecções Oportunistas Relacionadas com a AIDS/parasitologia , Criptosporidiose/diagnóstico , Ensaio de Imunoadsorção Enzimática/normas , Fezes/parasitologia , Infecções Oportunistas Relacionadas com a AIDS/diagnóstico , Animais , Estudos de Casos e Controles , Cryptosporidium/imunologia , Cryptosporidium/isolamento & purificação , Humanos , Valor Preditivo dos Testes , Reprodutibilidade dos Testes , Sensibilidade e EspecificidadeRESUMO
We evaluated the diagnostic performance of a Cryptosporidium immunoenzymatic assay (ELISA). Fecal samples were collected from 94 HIV-seropositive patients. All specimens were processed with a commercially-available ELISA to detect C. parvum specific coproantigen and with a modified Ziehl-Neelsen stain (ZNm) microscope exam. Overall, sensitivity of the immunoenzymatic test was 100 percent, with a specificity of 96 percent; positive and negative predictive values were 89 percent and 100 percent, respectively. The commercial ELISA and ZNm proved to be valuable diagnostic tools for Cryptosporidium infection.