RESUMO
In order to study the lesions produced by lymphocytic choriomeningitis virus (LCMV) adult guinea-pigs were inoculated intraperitoneally with 1000 PFU of the WE or the Armstrong strain of LCMV. The animals were sacrificed at 3, 7, 10 and 14 days p.i. and samples were taken for hematological, histopathological and virological studies. In the guinea-pigs infected with the WE strain there was destruction of the splenic red pulp with high titers of virus and different degrees of pneumonitis. The hematological studies showed lymphopenia from day 7 p.i. onwards and focal bone marrow necrosis. In the animals infected with the Armstrong strain there was a very moderate neutropenia and virus multiplication was less than 2 logs, comparing with WE, in every case. The presence of large numbers of polymorphonuclear neutrophil (PMN) in the splenic red pulp and in the lung infiltrate previous to the mentioned lesions, encourage the interpretation that the damage is somehow mediated by these cells. The guinea-pigs infected with the WE strain could represent a very convenient model to study the role of PMN in viral diseases, as well as the non-immune pathogenetic mechanisms for LCMV.
Assuntos
Exame de Medula Óssea , Coriomeningite Linfocítica/patologia , Baço/patologia , Animais , Cobaias , Coriomeningite Linfocítica/imunologia , Coriomeningite Linfocítica/microbiologia , Neutrófilos/microbiologia , Ratos , Baço/química , Baço/imunologia , Baço/microbiologia , Linfócitos T/microbiologiaAssuntos
Arenaviridae , Arenavirus do Novo Mundo , Doenças dos Roedores/microbiologia , Animais , Antígenos Virais/análise , Arenaviridae/imunologia , Arenaviridae/patogenicidade , Arenavirus do Novo Mundo/imunologia , Arenavirus do Novo Mundo/isolamento & purificação , Arenavirus do Novo Mundo/patogenicidade , Efeito Citopatogênico Viral , Reservatórios de Doenças , Feminino , Imunofluorescência , Febre Hemorrágica Americana/imunologia , Febre Hemorrágica Americana/patologia , Microscopia Eletrônica , Doenças dos Roedores/imunologia , Doenças dos Roedores/patologia , Roedores/microbiologiaRESUMO
Since the appearance of resistance to antiretroviral treatment is unavoidable, the host cell's transcription factor NF-kappaB is a novel HIV target. The goal of this study was to characterize the effect of two immunomodulators, curcumin (Cur) and sulfasalazine (Sul), with a protease inhibitor, indinavir (IDV), on HIV-1 persistently infected CD4+ T-cells. Viral p24 antigen production, viral infectivity (tested on MAGI cells) and viral relative infectivity (viral infectivity/p24) were analysed. When used alone, both immunomodulators were able to reduce viral infectivity. When in combination, both 10 microM IDV plus 10 microM Cur and 10 microM IDV plus 250 microM Sul showed a significant reduction in viral infectivity and viral relative infectivity when compared to the reduction produced by IDV alone. Thus, the use of immunomodulators with IDV could help to reduce HIV-1 production in persistently infected cells.
Assuntos
Fármacos Anti-HIV/farmacologia , Linfócitos T CD4-Positivos/virologia , Curcumina/farmacologia , HIV-1/efeitos dos fármacos , Indinavir/farmacologia , Sulfassalazina/farmacologia , Linhagem Celular , Sobrevivência Celular/efeitos dos fármacos , Sinergismo Farmacológico , Inibidores Enzimáticos/farmacologia , Proteína do Núcleo p24 do HIV/metabolismo , Inibidores da Protease de HIV/farmacologia , HIV-1/fisiologia , Humanos , Proteínas Serina-Treonina Quinases/metabolismo , Quinase Induzida por NF-kappaBRESUMO
When Vero cells infected with Junín virus are cultured in the presence of 16 mM glucosamine, no infectious virus is produced and no surface antigens or virions are detected. Cytoplasmic viral antigen, however, is synthesized normally.