First report of bacterial panicle blight (BPB) in Rice caused by Burkholderia gladioli in Bangladesh.

Bacterial panicle blight (BPB) has become one of the most destructive diseases of rice worldwide and Burkholderia gladioli and B. glumae are two major pathogens causing BPB (1). This disease causes several types of damage, most importantly grain spotting, rot, and panicle blight, which can result in yield losses of 75% or more (1,3). In recent years, symptoms including sheath rot, grain spotting, grain rot, and panicle blight have been observed in both inbred and hybrid rice varieties. These symptoms resemble those of BPB and cause cultivar-dependent yield losses. (3) also reported the same symptoms for BPB. To confirm the cause of the disease, 21 rice panicles (Haridhan, a local variety) with typical BPB symptoms were collected from a farmer's field in the region of Mymensingh, Bangladesh during the rainy season in mid-October, 2021. Due to the severity of the outbreak, the panicles became dark brown and produced chaffy grains; nearly 100% of the rice panicles in that field were severely infected. To identify the causal pathogen(s), 1g of rice grains from 20 plants with typical BPB symptoms were surface-sterilized by immersing them in 70% ethanol for a few seconds followed by sodium hypochlorite solution (3%) for 1min. The grains were then rinsed with sterilized distilled water three times. Surface-sterilized grains were then ground with a mortar and pestle; 5mL of sterile distilled water was added during grinding. The extracted suspension (20µL) was then either streaked or spread onto the selective medium (S-PG) (2). Bacterial colonies showing purple color on the S-PG medium were selected and purified as candidate pathogens. For molecular characterization, species specific primers targeting gyrB gene were used to perform PCR and resulted in 479bp as reported by (4). To verify further, the PCR products of 16SF & 16SR were amplified and sequenced partially producing around 1400bp (1) and five 16SF partial sequences were deposited into NCBI GenBank (OP108276 to OP108280). 16S rDNA and gyrB revealed almost 99% homology with Burkholderia gladioli (KU851248.1, MZ425424.1) and B. gladioli (AB220893, CP033430) respectively using BLAST analysis. These purified bacterial isolates produced a diffusible light-yellow pigment on King's B medium indicating toxoflavin production (3). The candidate five bacterial isolates were then confirmed by inoculating 10ml suspension 108CFU/mL into the panicles and sheaths of BRRIdhan28 in net house condition as described previously (1). All of the bacterial isolates obtained from the spotted rice grains produced light brown lesions on the inoculated leaf sheath as well as spotting on the grain. To fulfill Koch's postulates, the bacteria were re-isolated from the symptomatic panicles and were confirmed as B. gladioli by analyzing the sequences of gyrB and 16s rDNA genes. Taken together, these results confirmed that B. gladioli is responsible for causing BPB in the rice grain samples that we collected. To our knowledge, this is the first report of BPB caused by B. gladioli in Bangladesh and further research is necessary to develop an effective disease management technique, or else the production of rice will be severely hampered.

Rep-PCR Analyses Reveal Genetic Variation of Ralstonia solanacearum Causing Wilt of Solanaceaous Vegetables in Bangladesh.

Ralstonia solanacearum, a soil-borne and seed-borne plant pathogenic bacterium, causes bacterial wilt to several important crop plants causing substantial economic losses. To provide population information on this pathogen for developing effective control strategies, Rep-PCR was used to analyze the genetic variation of 18 representative isolates of R. solanacearum collected in Bangladesh. Phenotypic analyses revealed that all eighteen isolates belong to biotype 3 with wide diversity in aggressiveness on eggplant, tomato, and chili. Rep-PCR studies utilizing the REP, ERIC, and BOXIR primers showed a wide variation at the genetic level among the R. solanacearum isolates used in this study. Dendrogram constructed using REP, ERIC, and BOXIR primers based on banding patterns implied that R. solanacearum isolates were genetically diversified and distributed in four clusters at 83%, 80%, and 63% similarity index, respectively. The genetic relationship assayed by rep-PCR highlighted a wide range of genetic variation but no relation among geographical origin, aggressiveness, and phylogenetic groups of R. solanacearum isolates. These results conceded that other molecular markers related to virulence gene(s) might reveal the complex relationship among geographical origin, aggressiveness, and phylogenetic groups.

Identification of citrus greening based on visual symptoms: A grower's diagnostic toolkit.

Citrus greening is one of the world's most serious diseases of citrus that affects all cultivars and causes the systematic death of trees worldwide. The disease is caused mostly by the bacteria Candidatus Liberibacter species. The wind, rain, and touch of infected workers cannot transmit this infectious disease. The Asian Citrus Psyllid (ACP), a minuscule insect, is one of the main vectors that transmit the disease by feeding on citrus leaves. Citrus greening management is also quite expensive since no successful treatment for the cure has been found, except to remove all affected vegetables from healthy crops to eliminate the bacterial spread. Citrus greening is also the most challenging task, as signs of other citrus diseases and nutritional deficiencies are identical. The major signs of this disease are asymmetrical, blotchy mottling patterns on leaves. Here, several visible indications of citrus greening were addressed, which will enable farmers at the root level to detect and avoid this condition prior to its having a dramatic influence on citrus plantations. We also talked about the pen test method to determine symptoms as symmetrical or asymmetrical throughout the mid-vine, regardless of whether they are impacted by citrus greening or lack of nutrients.