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1.
Electrophoresis ; 39(7): 948-956, 2018 04.
Artigo em Inglês | MEDLINE | ID: mdl-29323408

RESUMO

Microwell arrays are widely used for the analysis of fluorescent-labelled biomaterials. For rapid detection and automated analysis of microwell arrays, the computational image analysis is required. Support Vector Machines (SVM) can be used for this task. Here, we present a SVM-based approach for the analysis of microwell arrays consisting of three distinct steps: labeling, training for feature selection, and classification into three classes. The three classes are filled, partially filled, and unfilled microwells. Next, the partially filled wells are analyzed by SVM and their tendency towards filled or unfilled tested through applying a Gaussian filter. Through this, all microwells can be categorized as either filled or unfilled by our algorithm. Therefore, this SVM-based computational image analysis allows for an accurate and simple classification of microwell arrays.


Assuntos
Análise em Microsséries/instrumentação , Análise em Microsséries/métodos , Imagem Óptica/métodos , Máquina de Vetores de Suporte , Algoritmos , Bioensaio/instrumentação , Bioensaio/métodos , Simulação por Computador , Corantes Fluorescentes/química , Luz
2.
Artigo em Inglês | MEDLINE | ID: mdl-37906497

RESUMO

This paper introduces an innovative approach for automated polyp segmentation in colonoscopy images, deploying an enhanced Pix2Pix Generative Adversarial Network (GAN) equipped with an integrated attention mechanism in the discriminator. Addressing prevalent challenges in conventional segmentation methods, such as variable polyp appearances, inconsistent image quality, and limited training data, our model significantly augments the precision and reliability of polyp segmentation. The integration of an attention mechanism enables our model to meticulously focus on the intricate features of polyps, improving segmentation accuracy. A unique training strategy, employing both real and synthetic data, is adopted to ensure the model's robust performance under a variety of conditions. The results, validated through rigorous tests on multiple public colonoscopy datasets, indicate a notable improvement in segmentation performance over existing state-of-the-art methods. Our model's enhanced ability to detect critical details early plays a pivotal role in proactive colorectal cancer detection, a key aspect of smart healthcare systems. This work represents an effective amalgamation of advanced AI techniques and the Internet of Medical Things (IoMT), signifying a noteworthy contribution to the evolution of smart healthcare systems. In conclusion, our attention-enhanced Pix2Pix GAN not only offers efficient and reliable polyp segmentation, but also showcases considerable potential for seamless integration into remote health monitoring systems, underlining the increasing relevance and efficacy of AI in advancing IoMT-enabled healthcare.

3.
Artigo em Inglês | MEDLINE | ID: mdl-37903039

RESUMO

This paper introduces a novel approach GPTFX, an AI-based mental detection with GPT frameworks. This approach leverages GPT embeddings and the fine-tuning of GPT-3. This approach exhibits superior performance in both classifying mental health disorders and generating explanations with accuracy of around 87% in classification and Rouge-L of around 0.75. We utilized GPT embeddings with machine learning models for the classification of mental health disorders. Additionally, GPT-3 was fine-tuned for generating explanations related to the predictions made by these machine learning models. Notably, the proposed algorithm proves well-suited for real-time monitoring of mental health by deploying in AI-IoMT devices, as it has demonstrated greater reliability when compared to traditional algorithms.

4.
Artigo em Inglês | MEDLINE | ID: mdl-37566510

RESUMO

People's health is adversely affected by environmental changes and poor nutritional habits, emphasizing the importance of health awareness. The healthcare system encounters significant challenges, including data insufficiency, threats, errors, and delays. To address these issues and advance medical care, we propose a secure healthcare prediction method, prioritizing patient privacy and data transmission efficiency. The Quantum-inspired heuristic algorithm combined with Kril Herd Optimization (QKHO) is introduced for healthcare prediction, along with a comparison to the Deep Forward Neural Network (DFNN) optimized using Krill Herd Optimization (KHO) and Quantum-inspired heuristic algorithm combined with Kril Herd Optimization. The proposed QKHO model outperforms conventional models and exhibits higher accuracy, precision, recall, and F1-score. Blockchain technology ensures secure data transmission to the server, surpassing the security level of existing RSA and Diffie-Hellman algorithms.

5.
J Indian Med Assoc ; 107(6): 345-6, 2009 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-19886370

RESUMO

Hyperhomocysteinaemia is rapidly emerging as an important risk factor for coronary artery disease, possibly because of its propensity to accelerate atherosclerosis. Whether it is also a risk factor for cerebrovascular accidents (CVA) is a matter of debate till now, as there are conflicting results of the various prospective studies. The present study was performed to correlate the levels of plasma homocysteine levels with that of ischaemic and haemorrhagic CVA. Forty-two cases of CVA were randomly selected over a period of one year, and their risk factors were assessed. It was observed that serum homocysteine levels were significantly raised in those with intracerebral infarcts when compared to those with intracerebral haemorrhage, although homocysteine levels didn't prove to be prognostically significant.


Assuntos
Hiper-Homocisteinemia/complicações , Acidente Vascular Cerebral/sangue , Doenças Cardiovasculares/sangue , Doenças Cardiovasculares/epidemiologia , Doenças Cardiovasculares/etiologia , Feminino , Humanos , Incidência , Luminescência , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Fatores de Risco , Acidente Vascular Cerebral/epidemiologia , Acidente Vascular Cerebral/etiologia
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