Complexity-functioning relationships differ across different environmental conditions.

Habitat complexity is widely considered an important determinant of biodiversity, and enhancing complexity can play a key role in restoring degraded habitats. However, the effects of habitat complexity on ecosystem functioning - as opposed to biodiversity and community structure - are relatively poorly understood for artificial habitats, which dominate many coastlines. With Greening of Grey Infrastructure (GGI) approaches, or eco-engineering, increasingly being applied around the globe, it is important to understand the effects that modifying habitat complexity has on both biodiversity and ecological functioning in these highly modified habitats. We assessed how manipulating physical (primary substrate) and/or biogenic habitat (bivalves) complexity on intertidal artificial substrata affected filtration rates, net and gross primary productivity (NPP and GPP, respectively) and community respiration (CR) - as well as abundance of filter feeders and macro-algae and habitat use by cryptobenthic fish across six locations in three continents. We manipulated both physical and biogenic complexity using 1) flat or ridged (2.5 cm or 5 cm) settlement tiles that were either 2) unseeded or seeded with oysters or mussels. Across all locations, increasing physical and biogenic complexity (5 cm seeded tiles) had a significant effect on most ecological functioning variables, increasing overall filtration rates and community respiration of the assemblages on tiles but decreasing productivity (both GPP and NPP) across all locations. There were no overall effects of increasing either type of habitat complexity on cryptobenthic fish MaxN, total time in frame or macro-algal cover. Within each location, there were marked differences in the effects of habitat complexity. In Hobart, we found higher filtration, filter feeder biomass and community respiration on 5 cm tiles compared to flat tiles. However, at this location, both macro-algae cover and GPP decreased with increasing physical complexity. Similarly in Dublin, filtration, filter feeder biomass and community respiration were higher on 5 cm tiles compared to less complex tiles. In Sydney, filtration and filter feeder biomass were higher on seeded than unseeded tiles, and fish MaxN was higher on 5 cm tiles compared to flat tiles. On unseeded tiles in Sydney, filter feeder biomass also increased with increasing physical complexity. Our findings suggest that GGI solutions via increased habitat complexity are likely to have trade-offs among potentially desired functions, such as productivity and filtration rates, and variable effects on cryptobenthic fish communities. Importantly, our results show that the effects of GGI practices can vary markedly according to the environmental context and therefore should not be blindly and uniformly applied across the globe.

Lotka-Volterra model with Allee effect: equilibria, coexistence and size scaling of maximum and minimum abundance.

The Lotka-Volterra competition model (LVCM) is a fundamental tool for ecology, widely used to represent complex communities. The Allee effect (AE) is a phenomenon in which there is a positive correlation between population density and fitness, at low population densities. However, the interplay between the LVCM and AE has been seldom analyzed in multispecies models. Here, we analyze the mathematical properties of the LVCM [Formula: see text] AE, investigating the coexistence of species interacting through neutral diffuse competition, their equilibria and stable points. Minimum viable population density arises as the threshold below which species go extinct, characteristic of strong Allee effects. Then, by imposing relationships of main parameters to body size, i.e. allometric scaling, we derive a general solution to the size-scaling maximum and minimum expected density under plausible scenarios. The scaling of maximum population density is consistent with the literature, but we also provide novel predictions on the scaling of the lower limit to population density, a critical value for conservation science. The resulting framework is general and yields results that increase our current understanding of how complex demographic processes can be linked to ubiquitous ecological patterns.

The teaching of offensive skills against different defensive scenarios in handball: analysis of coaches' perspectives in different categories.

As it is observed that the selection of teaching approaches takes an important role in youth players' development, investigations regarding teaching handball at this age reveal a gap due to this theme. This study aimed to identify and analyze the coaches' preferred offensive skills through different defensive scenarios and how they are taught in youth handball teams. Twenty-two Brazilian coaches from teams U-12, U-14, U-16 and U-18 were interviewed (semi-structured interview). Data analysis was performed through the Collective Subject Discourse and presented according to the teams and defensive systems faced. The coaches' discourses showed an increasing expectation throughout the age groups, and the expectation the players can deal with different situations based on their analysis. In addition, it was also found that teaching approaches are based on a technocratic way with further attempts to transfer the learned skills to the game context for all age groups. Therefore, it is suggested that greater attention must be paid while choosing skills and teaching approaches at each stage of the sports training process, in a way to stimulate youth players to deal critically and autonomously with game situations.

Environmental characterization of home range of Antillean manatees (Trichechus manatus) released in northeastern Brazil.

The Antillean manatee occurs discontinuously from the state of Amapá to the state of Alagoas on the coast of Brazil. There is also evidence of reintroduced manatees using the coasts of Sergipe and Bahia, with a preference for calm shallow waters. This study characterized the home range areas of six rehabilitated manatees released in northeastern Brazil. The activities were conducted in the states of Paraíba, Sergipe, and Bahia. Type of environment, substrate, depth, aquatic vegetation, physicochemical variables of the water, presence of solid waste, human settlements, and watercraft were considered to characterize the areas. The results showed a manatee preference for sheltered areas. Resources were available in larger quantities in the dry season, and a reduction in the availability of food items was fund over the years. High overlap was found in the multivariate space of the individuals in terms of the characteristics of the habitats. The estuary of the Paraíba River and the coastal area of Cabedelo Beach in Paraíba showed the greatest amount of solid waste, human settlements, and watercraft. Released manatees exhibited a preference for sites shallower than two meters, with food resources and fresh water availability.

The van der Waals interactions in systems involving superheavy elements: the case of oganesson (Z = 118).

This work presents a study involving dimers composed of He, Ne, Ar, Kr, Xe, Rn, and Og noble gases with oganesson, a super-heavy closed-shell element (Z = 118). He-Og, Ne-Og, Ar-Og, Kr-Og, Xe-Og, Rn-Og, and Og-Og ground state electronic potential energy curves were calculated based on the 4-component (4c) Dirac-Coulomb Hamiltonian and were counterpoise corrected. For the 4c calculations, the electron correlation was taken into account using the same methodology (MP2-srLDA) and basis set quality (Dyall's acv3z and Dunning's aug-cc-PVTZ). All calculations included quantum electrodynamics effects at the Gaunt interaction level. For all the aforementioned dimers the vibration energies, spectroscopic constants (ωe, ωexe, ωeye, αe, and γe), and lifetime as a function of the temperature (which ranged from 200 to 500 K) were also calculated. The obtained results suggest that the inclusion of quantum electrodynamics effects reduces the value of the dissociation energy of all hetero-nuclear molecules with a percentage contribution ranging from 0.48% (for the He-Og dimer) to 9.63% (for the Rn-Og dimer). The lifetime calculations indicate that the Og-He dimer is close to the edge of instability and that Ng-Og dimers are relatively less stable when the Gaunt correction is considered. Exploiting scaling laws that adopt the polarizability of involved partners as scaling factors, it has also been demonstrated that in such systems the interaction is of van der Waals nature (size repulsion plus dispersion attraction) and this permitted an estimation of dissociation energy and equilibrium distance in the Og-Og dimer. This further information has been exploited to evaluate the rovibrational levels in this symmetric dimer and to cast light on the macroscopic properties of condensed phases concerning the complete noble gas family, emphasizing some anomalies of Og.

Handball in school: teaching of defensive contents.

This study aimed to identify the defensive contents and teaching approaches preferred by handball coaches of school teams. Eleven coaches from a city in the State of São Paulo were interviewed. The discourses from the semi-structured interviews were analyzed based on the Collective Subject Discourse method. Coaches revealed that match-up, base position, coverage, and spatial occupation are the main individual defensive contents and use the teaching through games situations, technique approach, and teaching through games. The main group defensive content mentioned by the coaches is the match-up changes, mutual cover, and defensive systems, for which they preferred the teaching through games situations, teaching through games and technique approach. The findings show that although they are content of the same phase of the game, there is a preference for different teaching approaches. On the other hand, there seems to be inconsistency with the choice of the technique approach when it comes to the teaching of individual defensive contents, mainly because they are related to the players' decision-making. Such findings will contribute to the teaching of handball in school teams and with reflections for the teaching of the different specific contents, raising new discussions.

Seroprevalence of SARS-CoV-2 IgM and IgG antibodies in an asymptomatic population in Sergipe, Brazil.

OBJECTIVE: To estimate the prevalence of SARS-CoV-2 antibodies in an asymptomatic population in the state of Sergipe, Brazil.
. METHODS: This cross-sectional study with stratified sampling (sex and age) included serological immunofluorescent tests for IgM and IgG on samples from 3 046 asymptomatic individuals. Sample collection was performed in wet-markets of the 10 most populous cities of Sergipe, Brazil. Exclusion criteria included symptomatic individuals and health workers. The presence of comorbidities was registered.
. RESULTS: Of the 3 046 participants, 1 577 (51.8%) were female and 1 469 (48.2%) were male; the mean age was 39.76 (SD 16.83) years old. 2 921 tests were considered valid for IgM and 2 635 for IgG. Of the valid samples, 347 (11.9% [CI 10.7%-13.1%]) tested positive for IgM and 218 (8.3% [CI 7.2%-9.4%]) tested positive for IgG. Women over 40 had the highest prevalence for IgM (group C, p=0.006; group D p=0.04). The capital Aracaju displayed the highest prevalence for both antibodies; 83 (26.3% [CI 21.6%-31.6%]) tested positive for IgM and 35 (14.6% [CI 10.4%-19.7%]) for IgG. The most prevalent comorbidities were hypertension (64/123 individuals) and diabetes (29/123).
. CONCLUSIONS: A high prevalence of SARS-CoV-2 antibodies was found among asymptomatic persons in Sergipe. Women over 40 showed the highest rates. The capital, Aracaju, displayed the highest seroprevalence. Surveys like this one are important to understand how the virus spreads and to help authorities to plan measures to control it. Repeated serologic testing are required to track the progress of the epidemic.

OBJETIVO: estimar la prevalencia de anticuerpos dirigidos contra el SARS-CoV-2 en una población asintomática del estado de Sergipe, Brasil. MÉTODOS: estudio transversal con muestreo estratificado (por sexo y edad) que incluyó pruebas serológicas de inmunofluorescencia para IgM e IgG en muestras de 3 046 individuos asintomáticos. La recolección de muestras se realizó en los mercados húmedos de las 10 ciudades más pobladas de Sergipe, Brasil. Se excluyó a los individuos sintomáticos y a los trabajadores de la salud. Se registró la presencia de comorbilidades. RESULTADOS: De los 3 046 participantes, 1 577 (51,8%) eran mujeres y 1 469 (48,2%) varones; la edad promedio fue de 39,76 (SD 16,83) años. Se consideraron válidas 2 921 pruebas para la IgM y 2 635 para la IgG. De las muestras válidas, 347 (11,9% [CI 10,7%-13,1%]) resultaron positivas para IgM y 218 (8,3% [CI 7,2%-9,4%]) para IgG. Las mujeres mayores de 40 años tuvieron la mayor prevalencia de IgM (grupo C, p=0,006; grupo D, p=0,04). Aracaju, la capital del estado, mostró la mayor prevalencia para ambos anticuerpos; 83 (26,3% [CI 21,6%-31,6%]) resultaron positivas para IgM y 35 (14,6% [CI 10,4%-19,7%]) para IgG. Las comorbilidades más frecuentes fueron la hipertensión (64/123 individuos) y la diabetes (29/123). CONCLUSIONES: Se encontró una alta prevalencia de anticuerpos contra el SARS-CoV-2 en personas asintomáticas en Sergipe. Las mujeres mayores de 40 años mostraron las tasas más altas. La capital, Aracaju, mostró la mayor seroprevalencia. Las encuestas como esta son importantes para comprender cómo se propaga el virus y para ayudar a las autoridades a planificar medidas de control. Se requieren pruebas serológicas repetidas para dar seguimento al progreso de la epidemia.

Intrinsic Angiogenic Potential and Migration Capacity of Human Mesenchymal Stromal Cells Derived from Menstrual Blood and Bone Marrow.

Several therapies are being developed to increase blood circulation in ischemic tissues. Despite bone marrow-derived mesenchymal stromal cells (bmMSC) are still the most studied, an interesting and less invasive MSC source is the menstrual blood, which has shown great angiogenic capabilities. Therefore, the aim of this study was to evaluate the angiogenic properties of menstrual blood-derived mesenchymal stromal cells (mbMSC) in vitro and in vivo and compared to bmMSC. MSC's intrinsic angiogenic capacity was assessed by sprouting and migration assays. mbMSC presented higher invasion and longer sprouts in 3D culture. Additionally, both MSC-spheroids showed cells expressing CD31. mbMSC and bmMSC were able to migrate after scratch wound in vitro, nonetheless, only mbMSC demonstrated ability to engraft in the chick embryo, migrating to perivascular, perineural, and chondrogenic regions. In order to study the paracrine effects, mbMSC and bmMSC conditioned mediums were capable of stimulating HUVEC's tube-like formation and migration. Both cells expressed VEGF-A and FGF2. Meanwhile, PDGF-B was expressed exclusively in mbMSC. Our results indicated that mbMSC and bmMSC presented a promising angiogenic potential. However, mbMSC seems to have additional advantages since it can be obtained by non-invasive procedure and expresses PDGF-B, an important molecule for vascular formation and remodeling.

Influence of Match Location, Quality of Opponents, and Match Status on Movement Patterns in Brazilian Professional Football Players.

Aquino, R, Munhoz Martins, GH, Palucci Vieira, LH, and Menezes, RP. Influence of match location, quality of opponents and match status on movement patterns in brazilian professional football players. J Strength Cond Res 31(8): 2155-2161, 2017-The aim of this study was to examine the independent and interactive effects of match location, quality of opponents, and match status on the movement patterns in a professional Brazilian football team. Sixteen matches of the fourth division Brazilian Championship of 2015 were analyzed during the competitive stages (classifier, 8 matches; octave-finals, 2 matches; quarterfinals, 2 matches; semifinals, 2 matches; and finals, 2 matches). A 5-Hz Global Positioning System Sports QSTARZ was used to record the total distance (TD), maximum speed (VMAX), average speed (VAVERAGE), and frequency of high-intensity activities (HIA). The Student's t-test for independent samples showed significantly higher values (p ≤ 0.05) of VMAX, VAVERAGE, and HIA in home matches when compared with away matches. Comparing the quality of opponents, statistically higher values of TD, VMAX, and HIA were found when the team played against strong opponents. Regarding match status, 1-way analysis of variance demonstrated that when the team won presented significantly higher values of TD, VMAX, VAVERAGE, and HIA compared with matches when the team lost. There were no substantial interactive effects of match situational variables on movement patterns. Finally, multiple linear regression showed that the variable quality of opponents has a higher relative contribution to the variance in HIA (19%) than match status (16%) and match location (4%). In particular, the results indicate that physical performance in professional football is influenced by match situational variables, resulting in a change in the team's style of play.

Addressing Mental Health Needs in Low- and Middle-Income Countries: The case of São Caetano do Sul, Brazil.

São Caetano do Sul, a city in southeastern Brazil, boasts exemplary social indicators and healthcare services, with a population of 162,763 and a density of 9,736.03 inhabitants/km². Allocating 25% of its budget to healthcare, the city's mental healthcare services adhere to the National Mental Health Policy. Structured services include a CAPS-II, CAPS-AD, outpatient teams, and teams in various locations. Initiatives since 2000 include inaugurating a CAPS-AD in 2006, a psychiatric emergency service in 2010, and a CAPS-II in 2016, relocating CAPS-AD in 2019, and establishing the Cuca Legal Project in 2022. São Caetano do Sul has a Mental Health Risk Classification Protocol to aid clinical decision-making. Developing care lines for various groups, it offers programs like smoking prevention, school psychology, and obesity support. Collaborating with community centers, these facilities serve as teaching environments. The mental health care network focuses on five axes: Communication, Care, Prevention, Management, and Education, with specific proposed actions and competencies. Despite progress, challenges remain. Expanding access, reducing stigma, and implementing robust monitoring are crucial. São Caetano do Sul's experience offers valuable insights for similar urban settings in LMICs developing mental health programs.

Immediate improvement of motor function after epilepsy surgery in congenital hemiparesis.

Hemispherectomy often leads to a loss of contralateral hand function. In some children with congenital hemiparesis, however, paretic hand function remains unchanged. An immediate improvement of hand function has never been reported. A 17-year-old boy with congenital hemiparesis and therapy-refractory seizures due to a large infarction in the territory of the middle cerebral artery underwent epilepsy surgery. Intraoperatively, electrical cortical stimulation of the affected hemisphere demonstrated preserved motor projections from the sensorimotor cortex to the (contralateral) paretic hand. A frontoparietal resection was performed, which included a complete disconnection of all motor projections originating in the sensorimotor cortex of the affected hemisphere. Surprisingly, the paretic hand showed a significant functional improvement immediately after the operation. This observation demonstrates that, in congenital hemiparesis, crossed motor projections from the affected hemisphere are not always beneficial, but can be dysfunctional, interfering with ipsilateral motor control over the paretic hand by the contralesional hemisphere.

Turbidity shapes shallow Southwestern Atlantic benthic reef communities.

Southwestern Atlantic reefs (Brazilian Province) occur along a broad latitudinal range (∼5°N-27°S) and under varied environmental conditions. We combined large-scale benthic cover and environmental data into uni- and multivariate regression tree analyses to identify unique shallow (<30 m) benthic reef communities and their environmental drivers along the Brazilian Province. Turbidity was the leading environmental driver of benthic reef communities, with the occurrence of two main groups: clear-water (dominated by fleshy macroalgae) and turbid (dominated by turf algae). Seven out of 14 scleractinian coral species were more abundant in the turbid group, thus corroborating the photophobic nature of some Brazilian corals. The most abundant scleractinian in Brazil (Montastraea cavernosa), largely dominated (71-93% of total coral cover) both, the shallow turbid and deeper clear-water reefs. Because these habitat types are widely recognized as potential climate refuges, local threats (e.g. pollution, overfishing) should be averted.

Effects of different moist orange pulp inclusions in the corn grain rehydration for silage production on chemical composition, fermentation, aerobic stability, microbiological profile, and losses.

This study evaluated the effects of different moist orange pulp (MOP) inclusions in corn grain rehydration for silage production as a strategy to store and use MOP and whether these inclusions result in adequate fermentation and aerobic stability (AE) and acceptable losses. Ground corn grain and MOP were weighed separately and mixed to obtain MOP inclusions of 21%, 34%, or 42%. The control treatment was obtained with corn rehydrated with distilled water and MOP inclusion of 0%, with five laboratory silos (15-L buckets) per treatment. Acid detergent fiber, acid detergent insoluble protein, acid detergent lignin, lactic acid, acetic acid, and ethanol contents increased linearly with increasing MOP inclusion, whereas starch, in vitro dry matter (DM) digestibility and butyric acid contents and pH decreased linearly. NH3 -N/TN content reduced quadratically and was minimal with 8.34 g/kg DM at 14% MOP inclusion. Total DM losses increased quadratically with maximal losses of 4.26% DM at 25% MOP inclusion. AE reduced by 2.8 h for each 1% increase in MOP inclusion. Rehydration of corn grain with MOP results in an adequate fermentation process. The inclusion of 34% MOP was the best because DM losses were lower and AE was not drastically reduced.

Long-term monitoring projects of Brazilian marine and coastal ecosystems.

Biodiversity assessment is a mandatory task for sustainable and adaptive management for the next decade, and long-term ecological monitoring programs are a cornerstone for understanding changes in ecosystems. The Brazilian Long-Term Ecological Research Program (PELD) is an integrated effort model supported by public funds that finance ecological studies at 34 locations. By interviewing and compiling data from project coordinators, we assessed monitoring efforts, targeting biological groups and scientific production from nine PELD projects encompassing coastal lagoons to mesophotic reefs and oceanic islands. Reef environments and fish groups were the most often studied within the long-term projects. PELD projects covered priority areas for conservation but missed sensitive areas close to large cities, as well as underrepresenting ecosystems on the North and Northeast Brazilian coast. Long-term monitoring projects in marine and coastal environments in Brazil are recent (<5 years), not yet integrated as a network, but scientifically productive with considerable relevance for academic and human resources training. Scientific production increased exponentially with project age, despite interruption and shortage of funding during their history. From our diagnosis, we recommend some actions to fill in observed gaps, such as: enhancing projects' collaboration and integration; focusing on priority regions for new projects; broadening the scope of monitored variables; and, maintenance of funding for existing projects.

Feeding strategy of pelagic fishes caught in aggregated schools and vulnerability to ingesting anthropogenic items in the western equatorial Atlantic Ocean.

The present study aims to assess the diet composition of five commercial pelagic fishes caught in aggregated schools in the western Atlantic, quantify plastic and anthropogenic items in stomach contents, and analyze the possible relationship between their diet composition and the occurrence of anthropogenic items. We collected 807 stomachs from tunas (Thunnus albacares, Thunnus obesus, and Katsuwonus pelamis) and tuna-like species (Coryphaena hippurus and Elagatis bipinnulata) targeted by fisheries around data buoys in the western tropical Atlantic between 2011 and 2017. We quantified items of an anthropogenic origin by calculating frequency of occurrence. Feeding strategy was determined using a 3D scatterplot. Correspondence analysis (CA) was performed to determine the relationship between food items and the occurrence of anthropogenic materials (synthetic or organic). Fishing ropes and chum were the main items of an anthropogenic origin. Rainbow runner (E. bipinnulata) stomachs had the highest percentage of such items, whereas none were found in the bigeye tuna. The most affected species by anthropogenic items feed on epipelagic prey items, such as flying fishes. Organisms that aggregate around floating objects on the ocean surface and have a generalist diet are more likely to ingest synthetic items either accidently or actively. The present findings underscore the importance of environmental education programs to mitigate the impact of marine debris on organisms and ensure the health of individuals as well as the balance of the food web to which these species belong.

Ingestion of macroplastic debris by the common dolphinfish (Coryphaena hippurus) in the Western Equatorial Atlantic.

We report the occurrence of macroplastic debris in the stomach of a common dolphinfish (Coryphaena hippurus) caught in the Western Equatorial Atlantic. On the deck, we noticed that the fish was remarkably undernourished and decided to remove its stomach for laboratory analysis. A large part of a plastic bowl and a tuna pectoral fin were the only items recorded in the fish stomach. The plastic measured 99.57 cm2 and weighed 12.77 g, likely blocking the digestive tract and reducing food intake by the fish, as supported by signs of starvation. This is the record of the largest plastic debris ingested by a dolphinfish to date. As the source of the plastic container was probably a tuna fishing boat, we suggest strengthening environmental education programs to enhance the awareness of fishermen and mitigate the impacts of plastic pollution on the pelagic ecosystem and associated organisms, such as C. hippurus.

Remote sensing, isotopic composition and metagenomics analyses revealed Doce River ore plume reached the southern Abrolhos Bank Reefs.

On November 5th, 2015, the Fundão dam rupture released >50 million m3 of ore tailings into the Doce River, Minas Gerais State, Brazil. The huge volume of mud spread along the river and reached the sea, 17 days after the disaster, in Regência, Espírito Santo State (ES). In 2018, after three years of the disaster, the impacts of the ore tailings in the marine environment are still unclear. This study aims to investigate possible short-term impacts in marine biodiversity caused by the ore tailings' mud over the reef ecosystems that are closest to the disaster area: i.e. recently discovered reefs in the southern Abrolhos Bank. A remote sensing surveillance including winds, sea surface temperature, total suspended material and watercolor (MODIS Aqua data) indicated that the iron tailings plume reached the southern portion of Abrolhos Bank on June 16th, 2016. Subsequently, to obtain further evidence of the presence of the tailings in the coral reefs, water samples were collected in a gradient spanning from the river estuary to the reefs in southern Abrolhos Bank, we also analyzed the isotopic and microbial composition of the samples, as well as the reef benthic composition. Despite no clues of negative impact on benthic (coral) communities, isotopic analysis confirmed the presence of the plume over the reefs area. This study serves as a baseline for future long-term impact assessments of the health of coral reefs in the Abrolhos Bank.

Integrating Computational Methods to Investigate the Macroecology of Microbiomes.

Studies in microbiology have long been mostly restricted to small spatial scales. However, recent technological advances, such as new sequencing methodologies, have ushered an era of large-scale sequencing of environmental DNA data from multiple biomes worldwide. These global datasets can now be used to explore long standing questions of microbial ecology. New methodological approaches and concepts are being developed to study such large-scale patterns in microbial communities, resulting in new perspectives that represent a significant advances for both microbiology and macroecology. Here, we identify and review important conceptual, computational, and methodological challenges and opportunities in microbial macroecology. Specifically, we discuss the challenges of handling and analyzing large amounts of microbiome data to understand taxa distribution and co-occurrence patterns. We also discuss approaches for modeling microbial communities based on environmental data, including information on biological interactions to make full use of available Big Data. Finally, we summarize the methods presented in a general approach aimed to aid microbiologists in addressing fundamental questions in microbial macroecology, including classical propositions (such as "everything is everywhere, but the environment selects") as well as applied ecological problems, such as those posed by human induced global environmental changes.

Diretrizes de (con)federações nacionais para o desenvolvimento de treinadores de handebol em uma perspectiva de longo prazo / National (con)federations guidelines for the handball coaches development in the long-term overview / Directrices de las (con)federaciones nacionales para el desarrollo de entrenadores de balonmano en una perspectiva de largo plazo

Esta pesquisa teve como objetivo identificar e analisar as diretrizes de formação de treinadores de handebol em países que alcançaram resultados expressivos no contexto internacional nos últimos anos. Trata-se de uma pesquisa documental, realizada en-tre dezembro de 2020 e janeiro de 2021. Os dados foram extraídos dos endereços eletrônicos oficiais das respectivas (con)federações nacionais, por um instrumento elaborado pelos autores e, posteriormente, foram analisados por meio da análise temática. Os achados denotam o envolvimento das (con)federações na formação dos treinadores, que, por sua vez, apresentou características padronizadas e hierarquizadas, proporcionando diferentes níveis de treinadores, relacionados aos diversos contextos de atuação da modalidade (AU).

This study aimed at identifying and analyzing handball coaches' development guidelines in countries that got significant results in the international background in the last few years. It's a documental research performed between december 2020 and january 2021. The data was collected from the official websites of the respective national (con)federations using an instrument de-signed by the authors. It was analyzed through thematic analysis. The findings denote the engagement of the (con)federations in the coaches' development, which presented standardized and hierar-chical characteristics, providing different levels of coaches for each of the related fields of handball (AU)

Esta investigación tuvo como objetivo identificar y anali-zar las directrices de la formación de entrenadores de balonmano en naciones que lograron resultados expresivos en contexto inter-nacional en los últimos años. Se trata de una investigación docu-mental realizada entre diciembre de 2020 y enero de 2021. Los da-tos fueran obtenidos de las direcciones electrónicas oficiales de las (con)federaciones utilizando una herramienta confeccionada por los autores y después fue analizado mediante análisis temática. Los hallazgos denotan la participación de las (con)federaciones en la for-mación de entrenadores que, por su vez, presentó características estandarizadas y jerarquizadas proporcionando distintos grados de entrenador para cada ámbito de actuación en lo balonmano (AU).

Diretrizes de (con)federações nacionais para o desenvolvimento de treinadores de handebol em uma perspectiva de longo prazo / National (con)federations guidelines for the handball coaches development in the long-term overview / Directrices de las (con)federaciones nacionales para el desarrollo de entrenadores de balonmano en una perspectiva de largo plazo

Esta pesquisa teve como objetivo identificar e analisar as diretrizes de formação de treinadores de handebol em países que alcançaram resultados expressivos no contexto internacional nos últimos anos. Trata-se de uma pesquisa documental, realizada entre dezembro de 2020 e janeiro de 2021. Os dados foram extraídos dos endereços eletrônicos oficiais das respectivas (con)federações nacionais, por um instrumento elaborado pelos autores e, posteriormente, foram analisados por meio da análise temática. Os achados denotam o envolvimento das (con)federações na formação dos treinadores, que, por sua vez, apresentou características padronizadas e hierarquizadas, proporcionando diferentes níveis de treinadores, relacionados aos diversos contextos de atuação da modalidade.

This study aimed at identifying and analyzing handball coaches' development guidelines in countries that got significant results in the international background in the last few years. It's a documental research performed between december 2020 and january 2021. The data was collected from the official websites of the respective national (con)federations using an instrument de-signed by the authors. It was analyzed through thematic analysis. The findings denote the engagement of the (con)federations in the coaches' development, which presented standardized and hierar-chical characteristics, providing different levels of coaches for each of the related fields of handball.

Esta investigación tuvo como objetivo identificar y analizar las directrices de la formación de entrenadores de balonmano en naciones que lograron resultados expresivos en contexto internacional en los últimos años. Se trata de una investigación documental realizada entre diciembre de 2020 y enero de 2021. Los datos fueran obtenidos de las direcciones electrónicas oficiales de las (con)federaciones utilizando una herramienta confeccionada por los autores y después fue analizado mediante análisis temática. Los hallazgos denotan la participación de las (con)federaciones en la formación de entrenadores que, por su vez, presentó características estandarizadas y jerarquizadas proporcionando distintos grados de entrenador para cada ámbito de actuación en lo balonmano.