RESUMO
Gene expression products represent candidate biomarkers with the potential for early screening and therapy of patients with ovarian serous carcinoma. The present study, using patients that originate from the population isolate of South Tyrol, Italy, substantiates the feasibility of differential gene expression analysis in a genetically isolated population for the identification of potential markers of ovarian cancer. Gene expression profiles of fresh-frozen ovarian serous papillary carcinoma samples were analyzed and compared to normal ovarian control tissues using oligonucleotide microarrays complementary to 14,500 human genes. Supervised analysis of gene expression profiling data identified 225 genes that are down-regulated and 635 that are up-regulated in malignant compared to normal ovarian tissues. Class-prediction analysis identified 40 differentially expressed genes for further investigation as potential classifiers for ovarian cancer, including 20 novel candidates. Our findings provide a glimpse into the potential of population isolate genomics in oncological research.
Assuntos
Cistadenocarcinoma Seroso/genética , Neoplasias Epiteliais e Glandulares/genética , Neoplasias Ovarianas/genética , Ovário/metabolismo , Grupos Populacionais/genética , Biomarcadores Tumorais/genética , Biomarcadores Tumorais/metabolismo , Estudos de Casos e Controles , Cistadenocarcinoma Seroso/metabolismo , Cistadenocarcinoma Seroso/patologia , Feminino , Perfilação da Expressão Gênica , Regulação Neoplásica da Expressão Gênica , Humanos , Itália , Neoplasias Epiteliais e Glandulares/metabolismo , Neoplasias Epiteliais e Glandulares/patologia , Análise de Sequência com Séries de Oligonucleotídeos , Neoplasias Ovarianas/metabolismo , Neoplasias Ovarianas/patologia , Ovário/patologia , RNA Neoplásico/genética , RNA Neoplásico/metabolismoRESUMO
Fertiloscopy is a useful first level alternative procedure in order to diagnose infertility. It is a new diagnostic technique, which combines culdoscopy, dye-test, salpingoscopy and hysteroscopy and uses a specific disposable kit (Fertiloscopy Soprane). Fertiloscopy has the advantage of limiting pelviscopy only when strictly indicated, while maintaining a high diagnostic quality. Furthermore, it is more cost-effective by reducing the use of the operating theatre, the number of doctors and nurses involved and also by decreasing surgical risks and therefore leading to a better compliance by the patients.
Assuntos
Culdoscopia , Histeroscopia , Infertilidade Feminina/diagnóstico , Corantes , Feminino , HumanosRESUMO
The Hellp syndrome is a rare complication of hypertension in pregnancy, characterized by haemolysis, elevated liver enzymes and thrombocytopenia. Since the disease progresses very rapidly, it is important for successful treatment, to make an early diagnosis, evaluating the typical laboratory data changes, which are evident prior to clinical manifestations. Means of diagnosis and therapeutic approach are described, on the basis of personal experience.
Assuntos
Anemia Hemolítica/etiologia , Hipertensão/complicações , Complicações Cardiovasculares na Gravidez/diagnóstico , Trombocitopenia/etiologia , Feminino , Humanos , Fígado/enzimologia , Pré-Eclâmpsia/complicações , Gravidez , Complicações Cardiovasculares na Gravidez/terapia , SíndromeRESUMO
Water labor and/or birth are more and more requested and with the increased number of pregnant women choosing water-birth it is necessary to define advice for a safe use of this method of birth-assistance, in order to prevent any possible connected complication. The criteria for admission or exclusion to water labor and/or birth, correlated with maternal-fetal clinical aspects, hygienic aspects, legal-medicine aspects, are indicated. The structural, technical, organizing, specific requirements, in addition those of every delivery room, and considered important for water birth places, are underlined.
Assuntos
Parto Obstétrico/métodos , Imersão , Guias de Prática Clínica como Assunto , Água , Feminino , Humanos , Satisfação do Paciente , Gravidez , Fatores de RiscoRESUMO
The technical details are described for preparing and carrying out plasty of the ventral medial portion of the elevator ani. These are essential for a successful outcome of this stage of Lahodny's procedure for the surgical treatment of female urinary incontinence and descensus vaginae using an exclusively vaginal approach.
Assuntos
Incontinência Urinária/cirurgia , Prolapso Uterino/cirurgia , Canal Anal/cirurgia , Feminino , Humanos , Métodos , Músculos/cirurgiaRESUMO
BACKGROUND: To indicate the simplicity and the feasibility of this new diagnostic technique for infertility investigation. Ferti-test is a technique which combines dye-test and pelvic post coital test, using inexpensive surgical material. Ferti-test allows to perform laparoscopy only in very necessary cases, without loosing diagnostic quality. METHODS: Up to September 2001, 30 patients aged from 24 to 46 years, with infertile problems were enrolled. All the patients, in a preovulatory period, underwent dye-test combined with pPCT. Before the operation, all the patients were investigated for endocrinological assessment; an evaluation of sperm liquid in all the partners and a PCT have been carried out. All these data were organized in a specific diagram, which allowed to recognize the normality or the specific class of pathology of infertile couple. RESULTS: The purpose of this study is to remark the simplicity and the feasibility of this new diagnostic method. In our study, no patients who underwent dye-test and pelvic PCT had any complications. Only 7 patients with negative dye-test and pelvic PCT underwent diagnostic laparoscopy. CONCLUSIONS: The results of this study indicate that Ferti-test is an effective and reliable technique for the first level evaluation in infertile couples. Ferti-test is well tolerated and requires minimal cost. With Ferti-test it is possible to screen the patients who actually need laparoscopy and to reduce risks and costs.