Single-shot slightly off-axis digital holographic microscopy with add-on module based on beamsplitter cube.

Slightly off-axis digital holographic microscopy (SO-DHM) has recently emerged as a novel experimental arrangement for quantitative phase imaging (QPI). It offers improved capabilities in conventional on-axis and off-axis interferometric configurations. In this contribution, we report on a single-shot SO-DHM approach based on an add-on module adapted to the exit port of a regular microscope. The module employs a beamsplitter (BS) cube interferometer and includes, in addition, a Stokes lens (SL) for astigmatism compensation. Each recorded frame contains two fields of view (FOVs) of the sample, where each FOV is a hologram which is phase shifted by π rads with respect to the other. These two simultaneously recorded holograms are numerically processed, in order to retrieve complex amplitude distribution with enhanced quality. The tradeoff is done in the FOV which becomes penalized as a consequence of the simultaneous recording of the two holograms in a single snapshot. Experimental validation is presented for a wide variety of samples using a regular Olympus BX-60 upright microscope. The proposed approach provides an optimized use of the imaging system, in terms of the space-bandwidth product, in comparison with off-axis configuration; allows the analysis of fast-dynamic events, owing to its single-shot capability when compared with on-axis arrangement; and becomes easily implementable in conventional white-light microscopes for upgrading them into holographic microscopes for QPI.

Comparison of Seven Healthy Lifestyle Scores Cardiometabolic Health: Age, Sex, and Lifestyle Interactions in the NutrIMDEA Web-Based Study.

BACKGROUND: Global health status concerns both the assessment of wellbeing as well as the associated individualized determinants including quality of life and lifestyle factors. This study aimed to evaluate seven cardiometabolic health related scores and the influence, as well as interactions of lifestyle, heart-related and health-related quality of life (HRQoL) factors in order to inform the future implementation of precision public health (PPH). METHODS: Data collected from 17,333 participants who were enrolled of the NutrIMDEA study. The data collection period was between May 2020 and November 2020 through an online survey. The baseline questionnaire collected information on socio-demographic data, cardiometabolic history, anthropometric variables and lifestyle aspects. Also, physical and mental component scores of SF12 Health Survey (PCS12/MCS12) were assessed as HRQoL features, which were applied to estimated seven scores (LS7, HLS, 20-years DRS %, FBS, CLI, WAI derived, LWB-I). RESULTS: Most indices (except FBS, CLI, 20-years DRS % and WAI derived) showed that cardiometabolic outcomes and HRQoL measures were dependent on interactions by age and sex. The largest ponderal effect was found in PA total and Mediterranean Diet Score (MEDAS-14) interaction using LS7 as reference. However, using LWB-I as standard, the greatest effect was found in the quality-of-life feature MCS12. Noteworthy, LS7 showed good discrimination against PCS12, while LWB-I demonstrated excellent discrimination to MCS12. CONCLUSIONS: A major finding was the interplay between MEDAS-14 and PA on the LS7 scale as well as major effects of lifestyle factors and MCS12/PCS12 among scores, which need to be accounted with precision when implementing cardiometabolic screenings with PPH purposes.

Dual-mode holographic microscopy imaging platform.

We report on a novel layout capable of dual-mode imaging in real time with different magnifications and resolution capabilities in lensless microscopy. The concept is based on wavelength multiplexing for providing two illuminations with different wavefront curvatures: one is collimated, allowing a large field of view (FOV) with a poor resolution limit, and the other is divergent, to achieve a better resolution limit (micron range) over a small FOV. Moreover, our recently reported concept of MISHELF microscopy [M. Sanz, J. Á. Picazo-Bueno, L. Granero, J. García and V. Micó, Sci. Rep., 2017, 7, 43291] is applied to the divergent illumination case, improving the image quality by noise averaging and twin image minimization. The performance of this novel dual-mode lensless microscopy imaging platform is validated through experiments using both static amplitude (resolution test targets) and dynamic complex (flowing microbeads and live sperm cells) samples. The proposed imaging platform represents the background technology for a new generation of lensless microscopes with improved performance and capabilities.