Estimating the heritability of female lifetime fecundity in a locally adapted Drosophila melanogaster population.

The heritability of genome-wide fitness that is expected in finite populations is poorly understood, both theoretically and empirically, despite its relevance to many fundamental concepts in evolutionary biology. In this study, we used two independent methods of estimating the heritability of lifetime female fecundity (the predominant female fitness component in this population) in a large, outbred population of Drosophila melanogaster that had adapted to the laboratory environment for over 400 generations. Despite strong directional selection on adult female fecundity, we uncovered high heritability for this trait that cannot be explained by antagonistic pleiotropy with juvenile fitness. The evolutionary significance of this high heritability of lifetime fecundity is discussed.

Fungicide selective for basidiomycetes.

Concentrations of 2,3-dihydro-5-carboxanilido-6-methyl-1,4-oxathiin lower than 8 parts per million prevented mycelial growth of a number of Basidiomycetes. By contrast, mycelial growth of various other fungi-Phycomycetes, Ascomycetes, and Deuteromycetes-was 50 percent inhibited only by concentrations of 32 ppm or higher. Two exceptions to this pattern of selective fungitoxicity were found:an isolate of Rhizoctonia solani was not as sensitive as other Basidiomycetes, and the deuteromycete Verticillium alboatrum was inhibited by lower concentrations than affected other fungi in this group. Spore germination of two Basidiomycetes, Uromyces phaseoli and Ustilago nuda, was inhibited 95 percent or more at 10 ppm.

Alcohol consumption, %CDT, GGT and blood pressure change during alcohol treatment.

AIMS: Blood pressure (BP) changes in alcohol-dependent individuals during a 12-week alcohol relapse prevention study were examined in light of drinking status and biomarkers of alcohol consumption [carbohydrate-deficient transferrin (%CDT) and gamma-glutamyl transpeptidase (GGT)]. METHODS: Of 160 randomized alcoholic individuals, 120 who had hypertension and in whom daily drinking data was available, at 6 and 12 weeks of treatment were included. The impact of alcohol consumption on change in systolic BP (SBP) and diastolic BP (DBP) was examined. Further analysis determined the relationship between BP and alcohol-use biomarkers. RESULTS: A significant effect of complete abstinence on both SBP (-10 mmHg; P = 0.003) and DBP (-7 mmHg; P = 0.001) when compared to any drinking (SBP and DBP = -1 mmHg) was observed. At week 12, participants with a positive %CDT (> or =2.6) had 7 mmHg greater SBP (P = 0.01) and DBP (P < 0.001) than those with negative %CDT. Participants with positive GGT (> or =50 IU) had 10 mmHg greater SBP (P = 0.12) and 9 mmHg greater DBP (P = 0.03) than those with negative GGT. The percent change in SBP was correlated with percent change in %CDT (P = 0.003) but not GGT (P = ns). The percent change in DBP was correlated with both percent change in %CDT (P < 0.0001) and GGT (P = 0.03). CONCLUSIONS: Abstinence from alcohol significantly decreased the BP and a positive relationship between BP and both alcohol-use biomarkers was illustrated. Since %CDT is more specific than GGT for heavy alcohol consumption, clinicians may monitor the role of alcohol in hypertension using %CDT as a supplemental aid, providing an objective assessment of drinking to influence BP treatment decisions.

CO2 Exchange and Growth of the Crassulacean Acid Metabolism Plant Opuntia ficus-indica under Elevated CO2 in Open-Top Chambers.

CO2 uptake, water vapor conductance, and biomass production of Opuntia ficus-indica, a Crassulacean acid metabolism species, were studied at CO2 concentrations of 370, 520, and 720 [mu]L L-1 in open-top chambers during a 23-week period. Nine weeks after planting, daily net CO2 uptake for basal cladodes at 520 and 720 [mu]L L-1 of CO2 was 76 and 98% higher, respectively, than at 370 [mu]L L-1. Eight weeks after daughter cladodes emerged, their daily net CO2 uptake was 35 and 49% higher at 520 and 720 [mu]L L-1 of C02, respectively, than at 370 [mu]L L-1. Daily water-use efficiency was 88% higher under elevated CO2 for basal cladodes and 57% higher for daughter cladodes. The daily net CO2 uptake capacity for basal cladodes increased for 4 weeks after planting and then remained fairly constant, whereas for daughter cladodes, it increased with cladode age, became maximal at 8 to 14 weeks, and then declined. The percentage enhancement in daily net CO2 uptake caused by elevated CO2 was greatest initially for basal cladodes and at 8 to 14 weeks for daughter cladodes. The chlorophyll content per unit fresh weight of chlorenchyma for daughter cladodes at 8 weeks was 19 and 62% lower in 520 and 720 [mu]L L-1 of CO2, respectively, compared with 370 [mu]L L-1. Despite the reduced chlorophyll content, plant biomass production during 23 weeks in 520 and 720 [mu]L L-1 of CO2 was 21 and 55% higher, respectively, than at 370 [mu]L L-1. The root dry weight nearly tripled as the C02 concentration was doubled, causing the root/shoot ratio to increase with CO2 concentration. During the 23-week period, elevated CO2 significantly increased CO2 uptake and biomass production of O. ficus-indica.

A behavioral intervention program for chronic public drunkenness offenders.

A reinforcement contingency management system for ten chronic public drunkenness offenders was evaluated for short-term effects. Chronic inebriates were provided with required goods and services through skid row community agencies contingent on their sobriety. Intoxication resulted in a five-day suspension of all goods and services. Excessive drinking behavior was assessed by direct observations of intoxication and by randomly administered breath alcohol analyses. As a result of this intervention, subjects substantially decreased their number of public drunkenness arrests and their alcohol consumption, and increased their number of hours employed. No such changes were observed in a control group that received services on a noncontingent basis. Longer-term research studies of one to two years rather than a few months would be required before any widespread use of this approach would be warranted.

Treatment of essential tremor with methazolamide.

We treated 28 patients (16 women and 12 men) who had essential tremor with methazolamide. Their median age was 69 years (range, 34 to 89 years), and the median duration of tremor was 16 years (range, less than 1 to 69 years). Fifteen cases were familial and 13 were sporadic. Improvement in 10 patients who continued taking the drug ranged from moderate to complete relief. In addition, four patients had marked improvement and two had moderate improvement but discontinued use of the drug because of side effects. Five patients with a mild response and seven with no response also discontinued methazolamide therapy. The maximal mean daily dose was 203 mg for all patients and 129 mg (maintenance dose) for the patients who continued taking the drug. Side effects consisted primarily of somnolence, nausea, epigastric discomfort, anorexia, paresthesias, and numbness. No aplastic anemia was noted in any of the patients. The median duration of follow-up was 6 months (range, 10 weeks to 29 months). The therapeutic effect seemed unrelated to a family history of tremor, the effect of alcohol, or the responsiveness to propranolol or primidone. Methazolamide may be an effective drug in the treatment of some patients with essential tremor, particularly those with head and voice tremor.

Self-esteem, life stress and psychiatric disorder.

Using a special subsample from a survey of women in Edinburgh investigations were carried out into (a) which types of life event are associated with lowered self-esteem; (b) the role of life events and self-esteem in onset of psychiatric disorder; and (c) the additional significance of prior psychiatric consultation in determining onset. Stressors involving impaired relationships with others were the only ones clearly associated with lowered self-esteem. Minor psychiatric illness was predicted by stress of uncertain outcome, and, to a lesser extent, by impaired relationship stress. Onset of major depression was best predicted by an interaction between total stress experienced and low self-esteem. There was evidence that such onset involves a pre-existing low level of self-esteem on which life stress impinges, rather than life stress generating low self-esteem and then onset. A small group of subjects characterised by low self-esteem, prior psychiatric consultation and maladaptive coping seemed to be fluctuating in and out of psychiatric illness irrespective of stress.

Life events and other factors implicated in onset and in remission of psychiatric illness in women.

Life stressors for 574 Edinburgh women were assessed for uncertain outcome, impaired relationships and other characteristics. Thirteen weeks were covered either with no illness present or before a transient episode of Research Diagnostic Criteria (RDC)-defined anxiety/depression (duration less than 13 weeks) or before a longer episode or before illness remission or during continuing illness. Exploratory analysis suggested that stressors of uncertain outcome preceded longer illness onset. Impaired relationships went with continuing illness. Stressors with neither of these, and with fewer than two other characteristics, preceded transient illness. Remaining stressors predicted remission, as did ending of long-term difficulties. Self-esteem, support, coping, previous illness and marital status also discriminated between the groups.

Life events and the onset of affective disorder. A longitudinal general population study.

This paper examines the relationship between life events (ascertained by the Bedford College method) and the onset of affective disorder (defined according to the RDC scheme) in a longitudinal general population survey of women. Fall-off in the reporting of minor events is examined and discussed. Event rates, proportions of women challenged by events, and measures of the risk of RDC disorder associated with the experience of particular events are reported. The results based upon data from an initial interview were largely consistent with those based upon follow-up data, and underpinned earlier work. For both data sets, major difficulties were associated with illness onset. Severe dependent events showed stronger effects than severe independent events but both categories were rare. New substantive findings arising from short-term general population event research are unlikely.

Parkinson's disease monotherapy with controlled-release MK-458 (PHNO): double-blind study and comparison to carbidopa/levodopa.

The potent and selective dopamine D-2 agonist, MK-458 [PHNO; (+)-4-propyl-9-hydroxynaphthoxazine] was administered as monotherapy to nine patients with Parkinson's disease in a double-blind, placebo-controlled 12-week investigation; ten other patients were randomized to placebo. MK-458 was formulated as a controlled-release preparation, using a hydroxypropyl methylcellulose-lactase (HPMC) matrix. Patients receiving MK-458/HPMC improved on a variety of measures of parkinsonism, compared to their baseline scores; in contrast, only trivial improvement was seen within the placebo group. We subsequently compared the anti-Parkinson response to MK-458/HPMC with the response to chronic carbidopa/levodopa monotherapy in an open label trial. Carbidopa/levodopa improved parkinsonism to a significantly greater degree than MK-458/HPMC. Doses of MK-458 used in these studies (up to 60 mg per day) were substantially higher than those in previously reported preliminary studies of this medication. We conclude that monotherapy with MK-458/HPMC results in a significant anti-Parkinson effect; however, the response falls short of that seen with carbidopa/levodopa.

Seasonal progression of plant water relations in fynbos in the western Cape Province, South Africa.

Plant xylem pressure potentials and leaf conductances to water loss were measured in the spring and summer at two sites in the western Cape Province, Republic of South Africa. The measurements were to test the hypothesis that the fynbos of South Africa was equivalent to the chaparral of California, therefore a period of plant water stress, similar to the period measured in chaparral species, should occur in flynbos species during the dry summer. The measurements indicated little or no plant water stress in most fynbos species at both sites. The sites were similar in plant water relations in spite of a more than twofold difference in annual precipitation. The minor differences in plant water relations can be explained in terms of different vegetation cover and different vapor pressure deficits during the measurements. The results support the conclusion that the arid fynbos may be equivalent to chaparral, but the majority of the fynbos is equivalent to coniferous and broad-leaved evergreen forest in California.

Self-referral to primary care: symptoms and social factors.

Previous analyses of data obtained from a comparative study of Health Centre consulters and controls have indicated that the influence of social and demographic factors upon consulting behaviour is slight compared with that of symptom severity. However, the samples were very heterogeneous and some variables may be influential for certain sub-groups but not for others. Subjects were classified according to whether they thought their symptoms were caused by internal physical, external physical or psychological factors. For all groups the likelihood of consultation was highest for those who said they had no idea what caused their ill-health and for those who thought it had an internal physical cause. A significant interaction between marital status and attributed cause showed that the reduced likelihood of consultation amongst women living in a stable relationship with husband or cohabitee, did not apply to those who attributed their symptoms to an external physical cause. Changes in patients' concepts of causation following the consultation with their doctor supported the idea that the GP often succeeds in reassuring patients who cannot understand their symptoms or who, without reason, fear they may be suffering from serious physical illness.

Self-referral: social and demographic determinants of consulting behaviour.

This report, which is one of a series, is concerned with socio-demographic correlates of self-referral, and the extent to which the observed associations can be explained by variations in symptom prevalence and severity. Social class, marital status, employment status, and distance from health centre, all show small but significant associations with self-referral. For social class the effect appears to be mediated by symptoms. People in social classes IV and V, women who are widowed, divorced or separated, and people who live near the health centre are more likely to visit their general practitioner.

Dimensions of experience and symptomatology.

Six characteristics of life events and difficulties, namely loss (L), threat (T), anti-social act (A), hopeless situation (H), uncertain outcome (U) and choice of action (C), were used to score life situations experienced by 1060 adults over three months. Certain patterns of these, together with the respondents' sex, close and more superficial support discriminated significantly between subjects who had: depression the predominant symptom, anxiety predominant, tiredness predominant, backache predominant, none of these reaching pathological level. A hierarchy emerged from depression down through anxiety to tiredness and backache such that more severe life situations were associated with symptoms higher up the hierarchy. Situations with both choice of action (C) and loss (L) tended to be associated with depression. Anxiety related situations were mainly those containing threat (T) and at least two other characteristics. Tiredness went with situations characterised purely as CUH or CH or UH, and backache with minor situations containing only one characteristic. Lack of close confidant was most associated with depression; being a woman was associated with tiredness and anxiety equally and lack of superficial support with anxiety and depression equally. A parallel was drawn with Finlay-Jones and Brown.

Effect of parity on periparturient neutrophil function in dairy cows.

Impairment of periparturient neutrophil function in dairy cows has been described. This may contribute to increased susceptibility of cows to bacterial infections during the periparturient period. Susceptibility to many complications of the postpartum period is influenced by parity. The aim of this experiment was to determine whether parity influenced periparturient neutrophil function in Holstein cows. Cows (n = 46, of which 26 were calving for the first, second or third time, and 20 were of more advanced parity) were sampled during the last prepartum week, the first postpartum week, 3 weeks postpartum and 6 weeks postpartum. Neutrophils were isolated by centrifugation and hypotonic lysis of erythrocytes. Neutrophil superoxide anion production was determined by the superoxide dismutase inhibitable reduction of ferricytochrome c, and stimulus-induced shape change was determined at 0, 30, 60 and 120 s after exposure to zymosan activated bovine plasma. Superoxide anion production was significantly depressed in the first postpartum week in all cows. At this time, superoxide anion production was significantly lower in cows in their fourth or greater lactation than in cows in the first three lactations. Neutrophil shape change responsiveness was also influenced by parity; immediately prepartum, the shape change responsiveness differed between groups at 120 s after application of the stimulus, and during the first postpartum week shape change responsiveness was less in cows of advanced parity at 30, 60 and 120 s after application of the stimulus. We concluded that cows in the fourth or greater lactation suffered more profound periparturient impairment of neutrophil function than younger cows and that this may be a factor mediating their increased susceptibility to some postpartum complications.

Ethylenediaminetetra(methylenephosphonic acid): genotoxicity, biodistribution, and subchronic and chronic toxicity in rats.

Ethylenediaminetetra(methylenephosphonic acid), EDITEMPA, was tested for oral toxicity in rats in a 13-wk feeding study (at doses of 0, 5, 50 and 500 mg/kg/day) and in a chronic feeding study (at doses of 0, 4, 20 and 100 mg/kg/day). EDITEMPA was also tested for genotoxicity in the Ames, mouse lymphoma, unscheduled DNA synthesis, and in vivo cytogenetics assays. Additionally, absorption, distribution and excretion (ADE) studies were conducted following administration of [14C]EDITEMPA to rats by gavage and via the feed and drinking-water. The principal finding in the 13-wk study was mild anaemia in male and female rats given 500 mg/kg/day, which was resolved during a 9-wk recovery period. In the chronic study, there was no substantial evidence of any treatment-related toxicity or carcinogenicity. Differences in survival of control and treated females (noted late in the study) were interpreted to represent unusually good survival in controls; however, a compound-related increase in mortality could not be completely ruled out. Tests for genotoxicity were all negative. ADE studies revealed that [14C]EDITEMPA was poorly absorbed from the gastro-intestinal tract and that most of the absorbed dose was rapidly excreted by the kidneys or sequestered in bone. The gavage route of administration led to four- to six-fold increases in bone EDITEMPA levels as compared with administration in the feed and drinking-water, respectively. These results suggest that no significant toxicity or carcinogenicity concerns arise from EDITEMPA when it is administered in the feed at the concentrations tested. Reversible anaemia was seen only at very high doses and was interpreted as being secondary to EDITEMPA's ability to interfere with iron absorption and utilization. Localization of EDITEMPA in bone indicated a high degree of affinity for mineralizing tissues, consistent with its chelating properties. There was, however, no effect on bone resorption or mineralization. A comparison of human drinking-water levels of 3500 ppm EDITEMPA (based on a no-effect level of 100 mg/kg/day in rats) with the estimated worst-case exposure in humans of 0.01 ppm suggested a safety margin greater than 1 x 10(5).

Verbal and nonverbal marital interaction patterns of alcoholics and nonalcoholics.

In a study of the interaction of hospitalized alcoholic and nonalcoholic husbands and their wives, husbands tended to speak more during alcohol-related conversations, while wives spoke more when discussing other topics. Wives in both groups looked at their spouses more than their husbands did during alcohol-focused periods.

Emergence of alcohol expectancies in childhood: a possible critical period.

Previous investigations with adolescents (aged 12-19) have shown alcohol-related expectancies to develop in childhood prior to significant drinking experience and to covary directly with drinking behavior. To chart the development of alcohol expectancies in children as young as age 6, a procedure was developed to be as independent as possible of age-related variation in reading and language development. This instrument was administered to 114 elementary school children of both genders, distributed across grades 1 to 5. Psychometric analysis provided evidence of the test's reliability and validity. Evaluation of the developmental pattern produced two primary findings: (1) there was an overall trend of increasingly positive expectancies with age; and (2) strikingly, the bulk of the increase was observed in the third and fourth grades. Children's expectancies may be less differentiated than adolescent or adult expectancies. These findings suggest that the precursors for later alcohol use and abuse are formed in childhood and that prevention efforts may need to begin as early as third grade.