A dual streaming potential device used as an affinity sensor for monitoring hybridoma cell cultivations.

A dual streaming potential device was used for determining the content of monoclonal antibodies in cultivation medium for hybridoma cells. Samples of culture medium were analyzed as discrete pulses, as a continuous flow of constant concentration as well as with fluctuating concentrations. Tests were done with two subclasses of IgG as well as with IgM. Finally, the analytical device was applied to the registration of production of monoclonal antibodies in a cultivation.

Enantiomeric resolution of amino acid derivatives on molecularly imprinted polymers as monitored by potentiometric measurements.

Potentiometric measurements have been applied to the detection of enantiomeric separations on molecularly imprinted polymers. A flow-through column electrode, based on the use of polymers imprinted against L-phenylalanine anilide, is described. The electrode consisted of a glass column in which the polymer was packed and where the end frits constituted the electrodes. The flow stream potential across the column can be continuously recorded as solvent is pumped through the system. The column resolved the enantiomers of phenylalanine anilide as detected by both UV absorption and potentiometric measurements and the recorded signals could be correlated with the concentration of phenylalanine anilide. The calibration graphs obtained for the UV absorption of phenylalanine anilide were linear over the concentration range investigated, whereas the potentiometric signal was shown to be exponentially linear with concentration. The application of molecular imprints to the preparation of supports suitable for chromatographic separations of enantiomers and for the preparation of specific electrodes is discussed.