Assessment of cardiovascular risk in patients with metabolic syndrome working in the agricultural sector.

INTRODUCTION: Cardiovascular diseases are the main death cause in Poland. Several clinical studies showed association between metabolic syndrome and higher prevalence of diabetes mellitus, cardiac events and mortality. The aim of the study was to estimate cardiovascular complications and death risk in subjects with metabolic syndrome (MS) working in agriculture. MATERIAL AND METHODS: The study included 332 people working in agriculture in Lodz voivodeship, 231 with MS and 101 healthy ones. Increased risk of cardiovascular complications was determined for pulse pressure (pp) >63 mmHg. Based on the SCORE index, 10-year death risk due to cardiovascular complications was estimated taking into account sex, age, smoking, systolic blood pressure and total cholesterol concentration. A value ≥5% was accepted as high risk of death within 10 years. RESULTS: Increased risk of cardiovascular complications (pulse pressure >63 mmHg) was found in 31.60% subjects with MS and 6.93% healthy ones. CONCLUSIONS: High risk of cardiovascular complications and death occurs statistically more frequently in subjects with MS than in the rest of the population.

[Estimation of cardiovascular risk in patients with metabolic syndrome]. / Ocena ryzyka sercowo-naczyniowego u chorych z objawami zespolu metabolicznego.

Metabolic syndrome (MS) is a coexistence of metabolic risk factors affecting development of cardiovascular diseases. The SCORE system estimates fatal cardiovascular disease events over a ten-year period. AIM: The aim of the study was to estimate cardiovascular risk in patients with symptoms of MS. MATERIALS AND METHODS: The study included 268 patients with symptoms of MS according to International Diabetes Federation criteria (2005), 136 men and 132 women, mean age 59,62±9,21 years. The cardiovascular risk was estimated on based of pulse pressure and SCORE table in these patients. RESULTS: In subjects with MS the mean value of pulse pressure (pp) was 58,96±2,34 mmHg, increased values of pp was found in 33,21% subjects with MS. High and very high cardiovascular death risk occurred in 80,97% subjects with MS, significantly more often in men than women. CONCLUSIONS: In majority of patients with MS high cardiovascular risk was found. In every three subjects with MS high pulse pressure was stated.

[Estimation of plasma 25(OH)D vitamin level in residents of Lodz]. / Ocena stezenia witaminy 25(OH)D w osoczu mieszkanców Lodzi.

UNLABELLED: Recent evidence for the pleiotropic differentiated effects of vitamin D, coupled with recognition that vitamin D deficiency is common, has revived interest in this hormone. Vitamin D is produced by skin exposed to ultraviolet B radiation or obtained from dietary sources, including supplements. Persons commonly at risk for vitamin D deficiency include those with inadequate sun exposure, limited oral intake, or impaired intestinal absorption. THE AIM: of this study was to evaluate plasma 25(OH)D level in residents of Lodz. MATERIALS AND METHODS: The study included 326 residents of Lodz, 156 men and 170 women, aged 30-65 years (mean 57,25±8,24 years). The study group was divided according to the age and season. The concentration of 25-hydroxy vitamin D (25-OH-D) was assessed with the application of the LIAISON® test using chemiluminescent immunoassay (CLIA) technology. RESULTS: The mean plasma level of 25(OH)D was 24,96±9,84 ng/ml in studied population and it was not significantly higher in women than men (25,24 ±3,31 ng/ml vs. 23,97±3,71 ng/ml, p>0,05); The highest 25(OH)D concentration was noted in the oldest age group (29,12±2,78 ng/ml)and in summer months (33,21±2,91 ng/ml). CONCLUSIONS: The concentration of vitamin D in the plasma of residents of Lodz was significantly lower than its recommendations. Age and season of the year were the factors strongly affecting the level of 25(OH)D in studied group.

[Evaluation of prevalece of plasma 25(OH)D vitamin deficiency in residents of Lodz]. / Ocena czestosci wystepowania niedoborów witaminy 25(OH)D w osoczu mieszkanców Lodzi.

UNLABELLED: Only scarce data have been published regarding serum vitamin D status in adult population in Poland. Observational studies have suggested relationship between 25(OH)D deficiency and many diseases, such as cardiovascular diseases, autoimmunological diseases or cancers. Still little is known about plasma 25(OH)D deficiency in Polish healthy adults. THE AIM: The aim of the study was to assess plasma 25(OH)D vitamin deficiency in residents of Lodz. MATERIALS AND METHODS: The study included 326 residents of Lodz, 156 men and 170 women, aged 30-65 years (mean 57,25±8,24 years). The study group was divided according to the age and season. The plasma level of 25(OH)D above 30 ng/ml was considered normal, between 21 ng/ml and 30 ng/ml - suboptimal (hypovitaminosis) and below 20 ng/ml - insufficient (deficiency). RESULTS: Plasma 25(OH)D vitamin deficiency was observed in 23,01% participants, hipovitaminosis - in 46,32%, the recommended 25(OH)D concentration in the plasma was observed only in 30,67%. Plasma 25(OH)D vitamin deficiency was observed more often in men than women (25,64% vs. 20,59%, p<0,05);the highest percentage of patients with recommended 25(OH)D vitamin level was observed during summer months (60%). CONCLUSIONS: Plasma 25(OH)D vitamin deficiency was very high in residents of Lodz, especially in men, people over 55 years and during winter months.

[Estimation of plasma 25(OH)D vitamin deficiency in patients with metabolic syndrome]. / Ocena czestosci niedoborów witaminy 25(OH)D w osoczu chorych z objawami zespolu metabolicznego.

UNLABELLED: Cardiovascular diseases have been the main cause of mortality in Poland for many years, including premature death and the incidence is systematically growing. These diseases contribute to an increase in the number of disabled people and the cost of medical care. The problem of the so called metabolic syndrome (MS), which includes metabolic risks of atherosclerosis, has been known by doctors for a long time. Results of studies which have been conducted for some years confirm that vitamin D deficiency is a risk factor of MS disorders, including obesity, arterial hypertension, diabetes. AIM: The aim of the study was to assess plasma 25(OH)D vitamin deficiency in patients with MS. MATERIALS AND METHODS: The study included 268 patients with MS, 136 men and 132 women, aged 30-65 years (mean 59,62±9,21 years). The study group was divided according to the age and season. The plasma level of 25(OH)D above 30 ng/ml was considered normal, between 21 ng/ml and 30 ng/ml - suboptimal (hypovitaminosis) and below 20 ng/ml - insufficient (deficiency). RESULTS: Plasma 25(OH)D vitamin deficiency was observed in 80,97% patients with MS, hipovitaminosis - in 17,16%. The recommended 25(OH)D concentration in the plasma was confirmed only in 1,87%. Plasma 25(OH)D vitamin deficiency was detected more often in men than women (93,38% vs. 68,18%, p<0,05);the lowest percentage of patients with 25(OH)D vitamin deficiency was observed during summer months (47,14%). CONCLUSIONS: Plasma 25(OH)D vitamin deficiency was very high in patients with metabolic syndrome, especially in men, people over 55 years and during winter months.

Should antioxidant vitamin supplementation be applied in patients with metabolic syndrome? A case-control study.

INTRODUCTION: All cells in the human body are exposed to reactive oxygen species (ROS), which disturb the metabolic reactions in the organism. The antioxidant system in the human body consists of enzymatic and non-enzymatic mechanisms, among which vitamins A, C, and E play a major role. THE AIM OF THE STUDY: The aim of the study was to evaluate the supply of vitamins A, C, and E from daily food rations (DFR) in postmenopausal women with metabolic syndrome (MS) in relation to current nutrition standards. MATERIAL AND METHODS: The study involved 184 women with MS, aged 45-68 years (mean 57.38 ±8.17 years). The control group comprised 90 women, aged 41-65 years (mean 57.48 ±5.79 years) without MS. The food intake was assessed using 24-hour dietary recalls. RESULTS: The evaluation of intake of vitamins measured with daily food rations (DFR) demonstrated that the optimal level of 90-110% according to standards was achieved only in 3.62% of women with metabolic syndrome for vitamin A, in 8.88% for vitamin C, and in 11.41% for vitamin E, which was significantly less often found than in the control group (p < 0.001). CONCLUSIONS: Women with MS are characterised by diversified intake of vitamins A, C and E, and a subgroup of this patients present low level of antioxidant vitamins intake. Supplementation with antioxidant vitamins should be prescribed individually to postmenopausal women with MS.

Usefulness of Somatostatin Receptor Scintigraphy (Tc-[HYNIC, Tyr3]-Octreotide) and 123I-Metaiodobenzylguanidine Scintigraphy in Patients with SDHx Gene-Related Pheochromocytomas and Paragangliomas Detected by Computed Tomography.

AIMS: The aim of this study was to assess the usefulness of somatostatin receptor scintigraphy (SRS) using (99m)Tc-[HYNIC, Tyr3]-octreotide (TOC) and 123I-metaiodobenzylguanidine (mIBG) in patients with SDHx-related syndromes in which paragangliomas were detected by computed tomography and to establish an optimal imaging diagnostic algorithm in SDHx mutation carriers. METHODS: All carriers with clinical and radiological findings suggesting paragangliomas were screened by SRS and 123I-mIBG. Lesions were classified by body regions, i.e. head and neck, chest, abdomen with pelvis and adrenal gland as well as metastasis. RESULTS: We evaluated 46 SDHx gene mutation carriers (32 index cases and 14 relatives; 28 SDHD, 16 SDHB and 2 SDHC). In this group, 102 benign tumors were found in 39 studied patients, and malignant disease was diagnosed in 7 patients. In benign tumors, the sensitivity of SRS was estimated at 77% and of 123I-mIBG at 22.0%. The SRS and mIBG sensitivity was found to be clearly region dependent (p < 0.001). The highest SRS sensitivity was found in head and neck paragangliomas (HNP; 91.4%) and the lowest was found in abdominal paragangliomas and pheochromocytomas (40 and 42.9%, respectively). The highest 123I-mIBG sensitivity was found in pheochromocytomas (sensitivity of 100%) and the lowest in HNP (sensitivity of 3.7%). In metastatic disease, SRS was superior to mIBG (sensitivity of 95.2 vs. 23.8%, respectively). CONCLUSION: SRS and 123I-mIBG single photon emission computed tomography (SPECT) sensitivity in SDHx patients is highly body region dependent. In malignant tumors, SRS is superior to 123I-mIBG SPECT.

[Estimation of plasma 25(OH)D vitamin level in patients with metabolic syndrome]. / Ocena stezenia 25(OH)D w osoczu chorych z objawami zespolu metabolicznego.

UNLABELLED: Cardiovascular diseases are a major public health problem in developed and developing countries. A number of recent studies point to the pleiotropic differentiated effects of vitamin D, the deficiency of which positively correlates with the increased risk for cardiovascular diseases, hypertension, dyslipidemia, obesity or metabolic syndrome (MS). Vitamin D deficiency has also been proven to affect the progression of atherosclerosis. THE AIM: of this study was to evaluate plasma 25(OH)D level in patients with MS. MATERIALS AND METHODS: The study included 268 patients with MS, 136 men and 132 women, aged 30-65 years (mean 59,62±9,21 years). The study group was divided according to the age and season. The concentration of 25-hydroxy vitamin D (25-OH-D) was assessed with the application of the LIAISON® test using chemiluminescent immunoassay (CLIA) technology. RESULTS: The mean plasma level of 25(OH)D was 14,56±7,81 ng/ml in MS patients and it was not significantly higher in women than men (15,14 ±3,29 ng/ml vs. 13,97±3,41 ng/ml, p>0,05). The lowest 25(OH)D concentration was noted in the oldest age group (9,24±4,78 ng/ml). The highest 25(OH)D concentration was noted in summer months (23,71±3,82 ng/ml). CONCLUSIONS: The concentration of vitamin D in the plasma of patients with MS was significantly lower than its recommendations. Age and season of the year were the factors strongly affecting the level of 25(OH)D in MS patients.

[Physical activity in patients with symptoms of metabolic syndrome reduces the concentration of plasma antioxidant vitamins - protective effect of vitamin C]. / Aktywnosc fizyczna u chorych z objawami zespolu metabolicznego zmniejsza stezenia witamin antyoksydacyjnych w osoczu - ochronny efekt witaminy C.

UNLABELLED: Patients with cardiovascular diseases, including those with the symptoms of metabolic syndrome (MS), are recommended regular exercise but many studies indicate its role in the production of reactive oxygen species. Vitamin C supplementation may enhance the antioxidant barrier in MS patients. AIM: The aim of the study was to assess the impact of regular physical activity (PA)and vitamin C supplementation on plasma vitamin A, C and E levels in patients with MS. MATERIALS AND METHODS: The study included 62 patients with MS according to International Diabetes Federation criteria, 32 men and 30 women, aged 38-57 years (mean age 51,24 ± 5,29 years). The patients were divided in two groups: group I (MS+PA) - 31 patients with recommended regular physical activity; group II ( MS+PA+C) - 31 patients with recommended regular physical activity and vitamin C supplementation per os. The control group consisted of 23 healthy individuals without MS, 17 men and 6 women, aged 49-56 years (mean age 53,21 ± 3,6 years), who were not recommended any vitamin supplementation nor physical activity. Plasma vitamin A, C and E levels were estimated in MS patients with spectrophotometry using T60V spectrophotometer (PG Instruments) before and after regular exercise with and without vitamin C supplementation. In the control group plasma levels of antioxidant vitamins were assessed only once. RESULTS: The plasma vitamin A, C and E levels were significantly lower (p<0,05) in MS patients than in the control group. After 6 weeks of regular physical activity a significant fall in plasma levels of antioxidant vitamins was observed in MS patients. In the group of patients with regular physical activity and vitamin C supplementation there was detected a significant rise in the level of all the tested vitamins close to the levels in control group. CONCLUSIONS: Regular physical activity enhances the decrease in plasma antioxidant vitamin level in patients with MS. Vitamin C supplementation conducted in parallel with regular physical activity normalize plasma vitamin A, C and E levels in these patients.

Serum uric acid concentration is associated with early changes of glomerular filtration rate in patients with diabetes type 1 without increased albumin excretion.

UNLABELLED: The early loss of renal function in patients with type 1 diabetes may begin before proteinuria. Only 30% of patients with diabetes manifest overt proteinuria. According to the previous studies, increased urinary albumin excretion, which is considered a classic marker of progression of diabetic kidney disease, can regress to normal urine albumin excretion. The current studies conducted in patients with type 1 diabetes without increased urine albumin excretion showed that the uric acid concentration was an independent factor for the development of diabetic kidney disease. The aim of study was to assess the impact of uric acid concentration and to identify risk factors of the early glomerular filtration loss in patients with type 1 diabetes and normal urinary albumin excretion. MATERIALS AND METHODS: 147 patients (61 women and 86 men) with type 1 diabetes without increased urine albumin excretion were analysed. GFR (gromerular filtration rate) was estimated based on the serum cystatin C concentration. Centile charts were used to determine the variation of uric acid concentration depending on GFR and gender. RESULTS: The mean value of the filtration rate for the study group was 117 ml/min/m2. The uric acid level above 90th percentile in relation to GFR was diagnosed in 8.2% of women and 0% of men, between 90th and 50th percentile in 44.3 % of women and 5.8% of men and below 50th percentile in 47.5% of women and 94.2% of men. Contrary to men in women higher serum acid concentration was strongly associated with higher glomerular filtration rate. Hyperfiltraion was diagnosed in 15 of women and 19 of men. CONCLUSIONS: The high normal uric acid concentration in women with type 1 diabetes might play a crucial role in development of hyperfiltration.

[Estimation of plasma vitamin A, C and E levels in patients with metabolic syndrome]. / Ocena stezenia witamin A, C i E w osoczu chorych z objawami zespolu metabolicznego.

UNLABELLED: Metabolic syndrome (MS) is a coexistence of metabolic risk factors affecting development of cardiovascular diseases. In the pathogenesis of MS there participate reactive oxygen species which are excessively produced in such elements of MS as hyperglycemia, insulin resistance and obesity. Vitamins A, C and E are an important part of the non-enzymatic antioxidative barrier in humans. The aim of the study was to estimate plasma vitamin A, C and E levels in patients with symptoms of MS. MATERIAL AND METHODS: The study included 68 patients with symptoms of MS according to International Diabetes Federation criteria (2005), 37 men and 31 women, aged 34-65 years (mean age 57, 76 +/- 8, 29 years). The control group consisted of 24 healthy individuals without MS, 18 men and 6 women, aged 49-67 (mean age 58, 5 +/- 5, 6 years). Plasma vitamin A, C and E levels were estimated in patients and the control group with spectrophotometry using T60V spectrophotometer (PG Instruments). RESULTS: The plasma vitamin A, C and E levels were significantly lower (p < 0.05) in MS patients than in the healthy individuals without symptoms of MS. The most significant differences in the level of antioxidative vitamins in both groups were related to vitamin C and vitamin E. CONCLUSIONS: The decreased level of vitamins A, C and E points to the weakening of antioxidative barrier in patients with MS.

Assessment of 25(OH)D vitamin concentration in plasma of residents of Lodz with metabolic syndrome in pre- and postmenopausal period.

INTRODUCTION: Vitamin D deficiency is a risk factor for metabolic syndrome disorders and the occurrence of these disorders greatly contributes to the deficiency of vitamin D. Postmenopausal women are particularly prone to that deficiency. AIM: The aim of the study was to assess vitamin D concentration in the plasma of pre- and postmenopausal women, with or without metabolic syndrome. MATERIAL AND METHODS: The study included 141 women aged 26-77 (the mean age 58.74 years old), divided into 4 groups depending on the pre- or postmenopausal period and diagnosed or not with metabolic syndrome according to the International Diabetes Federation criteria (2005). Vitamin D concentration was assessed by LIAISON(®) test using chemiluminescent immunoassay (CLIA) technology. RESULTS: The mean vitamin D concentration was the highest among premenopausal women without metabolic syndrome (24.32 ng/ml), it was insignificantly higher than in postmenopausal women without metabolic syndrome (23.52 ng/ml) and significantly higher than in both groups with metabolic syndrome - premenopausal (19.86 ng/ml) and postmenopausal women (9.32 ng/ml). The recommended plasma 25(OH)D concentration was not found in any of postmenopausal women with diagnosed metabolic syndrome. CONCLUSIONS: Postmenopausal women with metabolic syndrome had a significantly lower 25(OH)D vitamin concentration in plasma than postmenopausal women without metabolic syndrome. The frequency of vitamin D deficiency in women with metabolic syndrome was very high, significantly higher than in women without metabolic syndrome.

Working memory impairment as a common component in recurrent depressive disorder and certain somatic diseases.

OBJECTIVES: Deterioration of the working memory is regarded as one of the most important deficits in a number of somatic diseases. The purpose of the present study was to compare the effectiveness of working memory in 4 groups of patients: 1) diagnosed with recurrent depressive disorder (rDD), 2) with diabetes type 1 (DM1), 3) with diabetes type 2 (DM2), 4) with arterial hypertension (HA) and in healthy controls (HC). METHODS: The study comprised 300 subjects: rDD (n=99), DM1 (n=31), DM2 (n=31), HA (n=30) and HC (n=109).Cognitive function assessment was based on Trail Making Test (TMT) and the Stroop test. RESULTS: Analysis of variance (ANOVA) indicated statistically significant differences of the mean values among particular groups for each of the analysed results of the Stroop Test and TMT (p<0.0001). Patients with DM1 performed better in both TMT and Stroop tests, when compared to those diagnosed with HA. Patients with HA obtained better results than patients with DM2. Patients with rDD performed significantly worse than those with DM1 in both parts of TMT (A/time: p=0.022, B/time: p<0.001) and in the Stroop test (RCNb/time: p<0.001; NCWd/time: p=0.001; NCWd/errors: p=0.443). They also obtained worse results than patients with DM2 and HA, however, the differences were not statistically significant. CONCLUSIONS: 1) Our study has confirmed previous results showing association between depressive disorder and cognitive impairment. 2) Patients with rDD had worse performance on working memory tasks than the patients with DM type 1, DM type 2 and HA. 3) Further investigation is needed to clarify the role of inflammatory and oxidative and nitrosative stress (O&NS) processes in neurocognitive dysfunctions occurring in recurrent depression and somatic disease.

Patient with lupus anticoagulant caused aPTT prolongation corrected with prednisolone treatment and later anticoagulation treatment due to chronic atrial fibrillation.

Key Clinical Message: Lupus anticoagulant caused aPTT prolongation in rare case can cause bleeding tendency especially when combined with other hemostasis abnormalities. In such cases, aPTT value can be corrected by immunosuppressants within several days of treatment. When anticoagulation therapy is needed vitamin K antagonist are a good option for the initial treatment. Abstract: Lupus anticoagulant antibodies despite causing aPTT prolongation are commonly associated with increased risk of thrombosis. We present a rare case of patient when these autoantibodies resulted in dramatic aPTT prolongation and combined with associated thrombocytopenia resulted in minor bleeding events. In presented case treatment with oral steroids resulted in aPTT values correction followed by resolution of bleeding tendency within several days. Later, the patient developed chronic atrial fibrillation and was started on anticoagulation treatment initially with vitamin K antagonist without bleeding complications during follow-up period. Corresponding changes in patient's aPTT time in a course of whole treatment is presented.

When "diabetic nephropathy" is not always of diabetic origin: a case report.

The etiology and pathogenesis of fibrillary glomerulonephritis (FGN) remains unknown. The presented case shows an extremely rare FGN in association with commonly diagnosed diabetes. A 74-year-old, non-smoking, obese and diabetic woman was hospitalized due to a progressive and accelerated decrease in the renal function. The primary cause of chronic kidney disease was believed to be of diabetic origin. In the renal biopsy, light microscopy showed glomerular changes resembling diabetic nephropathy, however electron microscopy evaluation revealed linear, randomly arranged fibrils present in the glomerular mesangium and in peripheral capillary loops. The biopsy confirmed fibrillary glomerulopathy.

Association of chemotactic cytokine receptor 5 (CCR5) gene polymorphism (59029 A/G, rs1799987) with diabetic kidney disease in patients with type 2 diabetes from Poland.

INTRODUCTION: Diabetic kidney disease (DKD) pathogenesis is multifactorial and is a combination of metabolic, genetic, and environmental factors. Due to a long period of asymptomatic course, it is often diagnosed late when advanced stages of the disease are present. Among patients with diabetes, the presence of chemotactic cytokine receptor 5 (CCR5) gene polymorphism is suspected to be associated with the risk of DKD occurrence; however, the results of the research conducted so far are inconclusive. The aim of this study was to evaluate the CCR5 gene polymorphism (rs1799987, 59029 A/G) association with DKD among patients with type 2 diabetes mellitus (T2DM), who are residents of the Upper Silesia region of Poland. MATERIAL AND METHODS: CCR5 gene polymorphism (rs1799987, 59029 A/G) was assessed among consecutive patients with type 2 diabetes mellitus (T2DM) treated in a single outpatient diabetology clinic in Upper Silesia, Poland. Its association with DKD was examined. Additionally, selected clinical and demographic data were included in the analysis. RESULTS: Among 467 eligible study patients, there was no association between examined CCR5 gene polymorphism and the presence of DKD in relation both to the American Diabetes Association definition (p = 0.6) and to the National Kidney Foundation definition (p = 0.3) of this complication. CONCLUSION: The presented study did not confirm the association between the examined gene polymorphism and the risk of DKD; further studies in this area are needed in order to establish or explicitly exclude this association.

Selected diabetes control indicators and working memory efficacy.

OBJECTIVES: The goal of the study was an evaluation of differences in working memory effectiveness between patients with type 1 and type 2DM. It was also attempted to ascertain whether the level of diabetes control is associated with working memory effectiveness. METHODS: 62 subjects were enrolled into the study. All patients were divided into two groups: patients with type 1DM (n=31) and with type 2DM (n=31). The Trail Making Test (TMT) and the Stroop Test were used for evaluation of working memory effectiveness. Diabetes control indicators included: glycated haemoglobin (HbA1C) level, total cholesterol concentration, HDL and LDL cholesterol concentration and body mass index (BMI). RESULTS: The patients with type 1DM obtained a significantly lower time in the execution of TMT, part B (p=0.01) and of RCNb (p=0.01) and NCWd (p=0.01) versions of the Stroop Test, while making significantly less errors in NCWd version (p=0.01). Significant correlations were demonstrated between BMI values and the rate of execution of TMT, part B (p=0.03), as well as the rate of execution of RCNb (p=0.04) and NCWd (p=0.01) versions of the Stroop Test. Total cholesterol level was significantly correlated with the rate of execution of TMT, part A (p=0.04) and B (p=0.01). No significant correlations were found between cholesterol fraction levels in blood of the studied patients and the results of performed tests, with one exception, regarding the relationship between LDL cholesterol fraction and the rate of the Stroop Test execution in RCNb version (p=0.04). CONCLUSIONS: 1). Higher working memory efficacy was demonstrated among the patients with type 1DM vs. those with type 2DM. 2). The level of diabetes control is an influential factor for working memory effectiveness in diabetic patients.

[An assessment of the antioxidant vitamins concentration in people with metabolic syndrome working in agriculture]. / Ocena stezenia witamin antyoksydacyjnych u osób z zespolem metabolicznym pracujacych w rolnictwie.

BACKGROUND: Vitamins A, C and E are important parts of the antioxidant barrier. Polish data on antioxidant vitamins deficiency in the population are rare, especially among physically active people with metabolic disorders. The aim of this study was to evaluate the serum concentrations of vitamins A, C and E in people with metabolic syndrome (MS) working in agriculture, the prevalence of their deficiency in these workers, and the correlation between antioxidant vitamins concentration and traditional biomarkers of cardiovascular diseases. MATERIAL AND METHODS: The study included 332 residents of the Lódz Voivodeship working in agriculture: 231 people with MS and 101 healthy ones. The serum concentrations of vitamins A, C and E were assessed using the chemiluminescent immunoassay technology. The antioxidant vitamins intake was assessed by means of a 24-h recall questionnaire using Diet 5.0 software. RESULTS: The mean serum concentrations of vitamins A, C and E were significantly lower in the MS workers than in the healthy ones No correlation was found between the antioxidant vitamins concentration and the dietary intake but there was a correlation between the antioxidant vitamins concentration and high-density lipoprotein cholesterol (HDL-C). A correlation was found between the serum concentrations of vitamins A and C, and vitamins A and E, in the MS workers. CONCLUSIONS: The serum concentrations of antioxidant vitamins in the MS workers were significantly lower than in the healthy controls, despite the similar physical activity level. The dietary intake had no impact on the serum concentrations of these vitamins. The HDL-C concentration in the MS workers correlated with the concentrations of all antioxidant vitamins. Med Pr. 2021;72(2):123-9.

[Assessment of 25(OH)D concentration in people with metabolic syndrome working in agriculture]. / Ocena stezenia 25(OH)D u osób z zespolem metabolicznym pracujacych w rolnictwie.

BACKGROUND: Polish data on vitamin D deficiency in the population are incomplete. Vitamin D deficiency seems to be common, but there is a lack of studies concerning the concentration of 25(OH)D in people with high UV exposure. The aim of this study was to evaluate the plasma concentration of 25(OH)D in people with and without metabolic syndrome (MS), working in agriculture, the prevalence of its deficiency in these workers, and the correlation between the plasma concentration of 25(OH)D and traditional biomarkers of cardiovascular diseases. MATERIAL AND METHODS: The study included 332 people working in agriculture in the Lódz voivodeship, including 231 people with MS and 101 healthy ones. The plasma concentration of 25(OH)D was assessed using the chemiluminescent immunoassay technology. The vitamin D intake was assessed using a 24-h recall questionnaire using Diet 5.0 software. RESULTS: The mean plasma concentration of 25(OH)D was 13.64±8.01 ng/ml in MS workers, and it was significantly lower than in the healthy ones (26.61±10.12 ng/ml, p < 0.00001); the highest concentration of 25(OH)D was noted in summer months. Deficient plasma concentrations of 25(OH)D were found significantly more often in MS workers than in the controls (81.82% and 20.79%, respectively, p < 0.00001). No correlation was found between the plasma vitamin D concentration and its dietary intake. The plasma concentration of 25(OH)D correlated with age of the examined workers (r = -0.28, p = 0.023), high density lipoprotein concentration (r = 0.19, p = 0.036) and glucose concentration (Rho = -0.24, p = 0.02). A multivariate analysis of variance demonstrated that the body mass index affected significantly the mean value of the 25(OH)D concentration in MS workers. CONCLUSIONS: The concentration of vitamin D in the plasma of workers with MS was significantly lower than in the healthy controls despite the same high UV exposure; these workers also manifested significantly higher 25(OH)D deficiency than the control subjects. This study indicates the need for further research on the concentration of 25(OH)D in people with metabolic disorders regardless of UV exposure and vitamin D intake with a diet. Med Pr. 2021;72(1):9-18.

No evidence for change in expression of TBC1D1 and TBC1D4 genes in cultured human adipocytes stimulated by myokines and adipokines.

TBC1D1 and TBC1D4 proteins play analogous, but not identical role in governing insulin-signalling pathway. Little is known about changes in expression levels of TBC1D1 and TBC1D4 genes in mammals, including humans. Number of factors were studied, but data remain controversial. The aim of this study was to evaluate the effect of selected cytokines, adipokines and myokines with known or putative insulin sensitivity regulation activity (adiponectin, irisin, omentin, interleukin 6, leptin, resistin, and tumour necrosis factor) on TBC1D1 and TBC1D4 expression levels in cultured differentiated human adipocytes. No significant differences were found between the adipocytes treated with different stimuli and this effect was determined not dose dependent. It is reasonable to conclude that relative shortage of data showing no change in TBC1D1 and TBC1D4 from literature results from publication bias; therefore, our finding provides additional insight into the role of both genes.