RESUMO
An efficient immune response to tularemia is dependent on a strong cell-mediated component. We tried to identify markers of cellular immune responses that indicate a vaccine efficacy against tularemia. BALB/c mice were immunized with mutant F. tularensis 15∆23A and/or F. tularensis 15 NIIEG strains and then were challenged i.n. with F. tularensis Schu. We compared the influence of F. tularensis antigens (tularinum) in vitro on production of IL-1, IL-5, IL-6, IL-17, IFN-γ, and TNF-α by splenocytes obtained from intact mice and mice immunized with mutant F. tularensis 15∆23A and/or F. tularensis 15 NIIEG strains. We also compared expression of CD28, CD154, TLR-2, and CD69 markers on CD4 and CD8 T-cells after activation with tularinum in vitro. We found that tularinum-induced CD4(+) T-cells increased TNF-α and IFN-γ synthesis and expression of CD69 only in group mice with high degree of post immunization protection against F. tularensis Schu challenge. Estimation of CD69 expression on CD3(+)CD4(+) cells and IFN-γ, TNF-α synthesis by CD4(+) T-lymphocytes could be useful for determination protect ability of antitularemia immunity.
Assuntos
Vacinas Bacterianas/administração & dosagem , Francisella tularensis/imunologia , Imunidade Celular , Linfócitos T/imunologia , Tularemia/prevenção & controle , Animais , Antígenos de Bactérias/imunologia , Antígenos de Bactérias/farmacologia , Antígenos CD/genética , Antígenos CD/imunologia , Vacinas Bacterianas/imunologia , Biomarcadores/metabolismo , Células Cultivadas , Expressão Gênica , Imunização , Imunofenotipagem , Interferon gama/genética , Interferon gama/imunologia , Interleucinas/genética , Interleucinas/imunologia , Camundongos , Camundongos Endogâmicos BALB C , Baço/citologia , Baço/efeitos dos fármacos , Baço/imunologia , Linfócitos T/citologia , Linfócitos T/efeitos dos fármacos , Receptor 2 Toll-Like/genética , Receptor 2 Toll-Like/imunologia , Tularemia/imunologia , Tularemia/microbiologia , Fator de Necrose Tumoral alfa/genética , Fator de Necrose Tumoral alfa/imunologia , Vacinas AtenuadasRESUMO
We report the draft genome sequences of three Francisella tularensis subsp. mediasiatica strains isolated in the Altai Territory, Russian Federation.