RESUMO
Transposition and homologous recombination of IS6110 appear in Mycobacterium tuberculosis along in vivo sequential infections. These events were checked in different clones of a successful strain, M. tuberculosis Zaragoza, with the focus on a variant in which integration of a copy of IS6110 in the origin of replication (oriC) region occurred.
Assuntos
Elementos de DNA Transponíveis , Evolução Molecular , Variação Genética , Mycobacterium tuberculosis/classificação , Mycobacterium tuberculosis/genética , Tuberculose/microbiologia , Análise por Conglomerados , Impressões Digitais de DNA , Genótipo , Humanos , Repetições Minissatélites , Tipagem Molecular , Mycobacterium tuberculosis/isolamento & purificação , Polimorfismo de Fragmento de Restrição , Recombinação Genética , Origem de ReplicaçãoRESUMO
The purpose of this study was to increase our knowledge about Mycobacterium africanum and report the incidence and characteristics of tuberculosis (TB) due to their lineages in Aragon, Spain, over the period 2003-2019. The study includes all the cases in our region, where all the M. tuberculosis complex isolates are systematically characterised. We detected 31 cases of M. africanum among 2598 cases of TB in the period studied. TB caused by M. africanum is rare (1.19%) in our population, and it affects mainly men of economically productive age coming from West African countries. Among the isolates, Lineage (L) 6 was more frequent than L5. The genotyping of these strains identified five clusters and 13 strains with a unique pattern. The isolates' characterisation identified a copy of IS6110 within the moaX gene, which turned out to be specific for L6. It will allow the differentiation of this lineage from the rest of MTBC with a simple PCR reaction. It remains to be established whether this polymorphism may limit M. africanum transmission. Furthermore, a mutation in the mutT2 promoter was found as specific for L6 strains, which could be related to the high variability found for L6 compared to L5.
Assuntos
Emigrantes e Imigrantes/estatística & dados numéricos , Mycobacterium tuberculosis/genética , Tuberculose/epidemiologia , África Ocidental , Proteínas de Bactérias/genética , Elementos de DNA Transponíveis/genética , DNA Bacteriano/genética , DNA Bacteriano/isolamento & purificação , Técnicas de Genotipagem , Humanos , Incidência , Tipagem Molecular/métodos , Mycobacterium tuberculosis/isolamento & purificação , Estudos Retrospectivos , Espanha/epidemiologia , Sulfurtransferases/genética , Tuberculose/diagnóstico , Tuberculose/microbiologiaRESUMO
OBJECTIVE: To investigate the serological diagnosis of congenital toxoplasmosis and the prevalence of antibodies against toxoplasma in pregnant women in our area. METHOD: Toxoplasmosis seroprevalence was analyzed in 68 712 serum samples from 47 635 pregnant women living in the catchment area of Hospital Miguel Servet during the period of 1992 to 2008. Detection of toxoplasma-specific immunoglobulins (IgM, IgA, IgG) and IgG avidity studies were carried out in the microbiology laboratory. RESULTS: Toxoplasmosis seroprevalence was 31.9%. The incidence of primary infection was 0.01% in susceptible pregnant women. Fifteen women seroconverted during pregnancy, and 9 of them had infants with congenital toxoplasmosis. CONCLUSIONS: The cases of congenital toxoplasmosis were detected by maternal seroconversion during pregnancy. IgA was the most sensitive marker for the detection of congenital infection in neonates.