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1.
J Med Primatol ; 52(3): 170-185, 2023 06.
Artigo em Inglês | MEDLINE | ID: mdl-37078442

RESUMO

BACKGROUND: Evaluation of blood parameters in captive non-human primates (NHPs) is crucial for monitoring their health and ensuring that their environment meets their physiological requirements. METHODS: We performed hemogram, serum biochemistry, and parasitological exams in 20 howler monkeys and 21 capuchin monkeys. RESULTS: In both species, over 50% of the individuals presented at least one parasite. There was a negative effect of age on red blood cell (RBC), white blood cell, platelets, total protein, globulin, and alkaline phosphatase, and a positive effect on the A:G ratio, gamma-glutamyl transferase, and mean platelet volume (MPV). Capuchin monkeys presented the highest platelets and alanine aminotransferase (ALT) values and howler monkeys presented the highest MPV, aspartate aminotransferase, ALT, amylase, glucose, bilirubin, and triglycerides values. We observed an interaction between species and sex on RBC, Htc, mean corpuscular hemoglobin concentration, and cholesterol. CONCLUSIONS: Species differences found in blood parameters may reflect differences in physiological adaptations associated with ecological and morphological traits and are clinically relevant for evaluating animal health and the suitability of breeding programs.


Assuntos
Alouatta caraya , Alouatta , Animais , Alouatta/fisiologia , Cebus , Sapajus apella , Eritrócitos
2.
J Med Primatol ; 51(2): 75-84, 2022 04.
Artigo em Inglês | MEDLINE | ID: mdl-34865231

RESUMO

BACKGROUND: Imaging diagnostic methods are essential for a complete understanding of the liver function and may aid in early detection of hepatic disorders. METHODS: Sixty-nine owl monkeys were grouped in three age groups (AG): AG1 (<2 years old, n = 14), 2 (2-6 years old, n = 21), and 3 (>7 years old, n = 34). The animals were evaluated by ultrasound, liver function tests, and hemogram. RESULTS: The ultrasound revealed abnormal findings in the parenchyma ("starry sky" and steatosis) and/or in the gallbladder (cholelithiasis, septum, and wall edema) in 17 individuals, with higher incidence in AG3 individuals. Seven hepatic individuals showed alterations in the blood tests. CONCLUSION: Our study reported the occurrence of abnormal liver findings by ultrasound in owl monkeys and their associated clinical analyses. We recommend the regular use of ultrasound to evaluate liver condition in owl monkeys, particularly in old individuals.


Assuntos
Aotidae , Hepatopatias , Animais , Vesícula Biliar/diagnóstico por imagem , Hepatopatias/diagnóstico por imagem , Hepatopatias/veterinária , Ultrassonografia/veterinária
3.
Trop Anim Health Prod ; 53(2): 273, 2021 Apr 20.
Artigo em Inglês | MEDLINE | ID: mdl-33880646

RESUMO

This research aimed to obtain a hematological and biochemical profile of buffalos (Bubalus bubalis) bred in a region of the Brazilian Legal Amazon. A total of 73 animals of mixed races, divided into three groups, were studied: group 1 (G1) comprised animals up to 11 months old; in group 2 (G2), subjects were 12 to 23 months old; and, in group 3 (G3), they were 24 months old and older. We performed the hematological analysis manually, and, for the biochemistry, we used biochemical analyzers. Age of the animals has influenced the monocyte count. The counting of these cells was higher (p > 0.05) in younger animals (G1). There was no difference (p > 0.05) in hematological parameters concerning sex. In regard to biochemistry, we found that age influenced results for albumin and urea. Animals from G1 had higher albumin values when compared to G2 and G3. Serum urea values were higher in animals from G3. Regarding the serum mineral parameters, there were no significant results when the different age groups and both sexes were compared. Thus, the hematological and biochemical values obtained can work as a reference for the bubaline species of animals bred in the studied region, under the same management and breeding conditions. Sex and age of the animals are necessary for the interpretation of the tests.


Assuntos
Bison , Hematologia , Animais , Brasil , Búfalos , Feminino , Masculino , Valores de Referência
4.
Mol Biol Rep ; 46(4): 3617-3623, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31201676

RESUMO

The callitrichids are non-human primates that feed on insects and plant matter in nature, but in captivity, they are fed mostly an artificial diet containing amounts of gluten, in their toxic forms in items such as wheat, barley and rye. The aim of this research was to estimate the blood ß-defensin and Toll like receptor 5 (TLR5) gene expressions and to analyze the stool consistency (firm, soft, diarrheic) in Leontocebus fuscicollis raised in captivity. Blood samples of animals under gluten-free and gluten diets were collected and their fecal output quality was periodically monitored and classified during the course of the study. Gene expression was evaluated using real-time PCR. The stool consistencies of individuals fed a gluten diet were most frequently soft or diarrheic, while it was mostly normal in individuals fed a gluten-free diet. ß-Defensin expression increased in individuals fed a gluten diet, but decreased after 15 days. Expression normalized between 30 and 45 days on a gluten-free diet. However, expression of the TLR5 gene did not change under a gluten diet. A gluten diet affects stool quality, and brings about an immediate increase in blood ß-defensin expression in the beginning but decreases after 15 days.


Assuntos
Dieta Livre de Glúten , Expressão Gênica/imunologia , Glutens/metabolismo , Animais , Callitrichinae , Diarreia , Fezes , Imunidade Inata , Inflamação , Receptor 5 Toll-Like/sangue , beta-Defensinas/sangue
5.
Rev Bras Med Trab ; 21(1): e2023818, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-37197342

RESUMO

Introduction: Safety in health care should be a priority of all health professionals. The occurrence of occupational accidents is many times attributed to a failure in following established standards, and identifying and correcting the risks to which professionals are exposed is important. Objectives: The aim of this study was to assess the level of understanding on the biological hazards to which workers of a clinical analysis laboratory are exposed. Methods: We applied a questionnaire for assessing knowledge on biological hazards, comprising an assessment of the understanding of biosafety and biological hazards, an investigation of the occurrence, types, and causes of accidents with biological material, and the employment of preventive measures. Data were tabulated in spreadsheets. All qualitative variables were tested with the chi-square test. Results: We verified that 100% of the workers reported having some knowledge on biosafety; 25% of them reported they had suffered an occupational accident; and 81% of the workers reported having received training on biosafety measures. As to the level of exposure of workers and the community to biological agents, we noticed a very low level of exposure in one of the laboratory sectors. Conclusions: Considering our results, we concluded that professionals at a clinical analysis laboratory are prone to occupational hazards, facing a low risk of exposure despite carrying out hazardous activities with potential exposure, which requires caution and exposure prevention measures.


Introdução: A segurança na assistência médica deve ser uma prioridade de todos os profissionais da área da saúde. Muitas vezes, a ocorrência de acidentes de trabalho é atribuída ao não seguimento dos padrões definidos, sendo importante identificar e corrigir os riscos a que os profissionais estão expostos. Objetivos: O objetivo deste estudo foi avaliar o nível de compreensão sobre os riscos biológicos a que os trabalhadores de um laboratório de análises clínicas estão expostos. Métodos: Foi aplicado um questionário para avaliação sobre o conhecimento dos riscos biológicos, composto por avaliação sobre o conhecimento dos conceitos de biossegurança e riscos biológicos; investigação da ocorrência, dos tipos e das causas de acidentes com materiais biológicos; e uso de medidas preventivas. Os dados foram tabulados em planilhas. Todas as variáveis qualitativas foram testadas usando o teste do qui-quadrado. Resultados: Verificou-se que 100% dos trabalhadores relataram que possuem conhecimento sobre biossegurança; 25% relataram já ter sofrido algum acidente em serviço; e 81% dos trabalhadores relataram que receberam treinamento sobre as medidas de biossegurança. Quanto ao nível de exposição dos trabalhadores e da comunidade aos agentes biológicos, constatou-se um nível de exposição muito baixo em um dos setores do laboratório. Conclusões: Diante do exposto, conclui-se que os profissionais de laboratório de análises clínicas têm predisposição aos riscos ocupacionais, enfrentando um risco baixo de exposição apesar de desempenharem atividades insalubres e com potencial de exposição, o que requer cuidados e medidas de prevenção à exposição.

6.
Am J Primatol ; 74(5): 482-90, 2012 May.
Artigo em Inglês | MEDLINE | ID: mdl-22511524

RESUMO

This study aimed to characterize anatomical and biochemical properties of owl monkey kidneys in order to provide normal reference values. Sixty-nine Aotus azarai infulatus (45 males and 24 females) were divided into four different age groups (AG1: 3 months-1 year; AG2: 2-3 years; AG3: 4-6 years; and AG4: over 7 years old). The monkeys were evaluated with a serum chemistry profile, focusing on serum creatinine (SCr) and blood urea nitrogen (BUN) and with ultrasound. Mean body mass differed among the age groups. This significance was attributed to AG1 body mass being significantly lower than in AG2 and that in both AG2 and AG3 being significantly lower than in the two older age groups (AG3 and AG4). SCr and BUN concentrations differed significantly between the sexes and SCr level correlated positively with age. In contrast, renal measurements did not differ between males and females. Left and right renal volumes did not differ significantly within age groups, or among AG2, AG3, and AG4. Renal volumes in AG1, however, while not differing from those in AG2, did differ significantly from those in AG3 and AG4. In conclusion, this study provides ultrasonographic reference values for the morphology the kidneys in A. a. infulatus. Evidence is also provided that SCr and BUN levels in owl monkeys are influenced by the sex and age of the individual, factors that should be considered when interpreting test results.


Assuntos
Aotidae/anatomia & histologia , Aotidae/sangue , Nitrogênio da Ureia Sanguínea , Creatinina/sangue , Rim/anatomia & histologia , Rim/diagnóstico por imagem , Distribuição por Idade , Animais , Feminino , Masculino , Exame Físico/normas , Exame Físico/veterinária , Padrões de Referência , Distribuição por Sexo , Ultrassonografia
7.
J Ethnopharmacol ; 111(2): 378-82, 2007 May 04.
Artigo em Inglês | MEDLINE | ID: mdl-17254726

RESUMO

Lippia sidoides in Northeastern Brazil is widely used in the social medicine program named "Live Pharmacies" run by the municipal governments of country towns, to help poor people with phytotherapy, performed with local plants that are inexpensive but very effective. This plant is mainly used as a general antiseptic due to its strong action against many microorganisms. In order to evaluate the action spectrum of this plant, pharmacological studies were performed on acute toxicity, topical inflammation and ethanol-induced gastric lesions in mice, using the leaf essential oil (EO) of Lippia sidoides. The topical application of EO at doses of 1 and 10mg/ear, respectively, significantly reduced (P<0.05) in 45.93 and 35.26% the acute ear edema induced by 12-otetradecanoylphorbol 13-acetate (TPA). The gastroprotective effect was demonstrated by oral pretreatment with EO at doses of 10, 50 and 100mg/kg, which, respectively, significantly inhibited (P<0.05) by 58.33, 45.83 and 41.66% the damage produced by ethanol, but altered neither the weight nor the protein gastric mucus induced by ethanol administration. This study confirmed the great potential of this plant for medicinal proposals.


Assuntos
Anti-Inflamatórios/farmacologia , Antioxidantes/farmacologia , Lippia/química , Óleos Voláteis/farmacologia , Folhas de Planta/química , Gastropatias/tratamento farmacológico , Administração Oral , Administração Tópica , Animais , Anti-Inflamatórios/uso terapêutico , Relação Dose-Resposta a Droga , Avaliação Pré-Clínica de Medicamentos , Etanol/toxicidade , Feminino , Camundongos , Óleos Voláteis/administração & dosagem , Óleos Voláteis/uso terapêutico , Fitoterapia , Plantas Medicinais , Gastropatias/etiologia , Testes de Toxicidade Aguda
8.
Vet Clin Pathol ; 46(1): 126-131, 2017 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-28165629

RESUMO

BACKGROUND: The combined use of renal biochemistry and ultrasonographic imaging may improve the correct management of renal disease. Although renal disease is frequently observed in nonhuman primates, renal function markers have not yet been studied in Squirrel monkeys (Saimiri collinsi). OBJECTIVES: The aim of this study was to establish normal renal biochemistry variables and ultrasonographic features in Squirrel monkeys. MATERIAL AND METHODS: Renal biochemistry variables and ultrasonographic images were documented in 29 healthy Squirrel monkeys (15 males and 14 females). Urea, serum creatinine (SCr), and uric acid (UA) concentrations were measured by kinetic assay. Cystatin C (CysC) was analyzed by immunonephelometry. A multiple frequency linear array probe (5-12 MHz) was used for ultrasonographic imaging. The studied indicators of renal function were related to sex, age, and body mass. RESULTS: Serum creatinine was influenced by sex and body mass. Serum concentration of urea, UA, and CysC were not influenced by sex, age, and body mass. Ultrasonographic images provided accurate and comprehensive data for making clinical decisions for Squirrel monkeys. The total renal volume was only influenced by the body mass nested in sex and was positively correlated to body mass. Right renal volume was bigger than the left one. CONCLUSION: Normative standards for the renal evaluation, including biochemistry and ultrasonography, in the Squirrel monkey have been established correlated to age, sex, and body mass.


Assuntos
Cistatina C/análise , Animais , Biomarcadores/análise , Feminino , Rim/química , Rim/diagnóstico por imagem , Masculino , Saimiri , Ultrassonografia/veterinária
9.
Rev. bras. ciênc. vet ; 28(4): 211-217, out./dez. 2021. il.
Artigo em Português | LILACS, VETINDEX | ID: biblio-1363493

RESUMO

O objetivo do presente estudo foi validar intervalos de referências (IRs) para hematologia e bioquímica sanguínea de cães domiciliados da Amazônia Oriental e estabelecer novos IRs para os parâmetros não validados. Foram utilizadas amostras de 44 cães adultos clinicamente saudáveis de diferentes raças e sexos. Na validação, foi utilizada a metodologia proposta pelo Clinical and Laboratory Standards Institute (CLSI) e na determinação dos IRs dos parâmetros não validados foi utilizada a metodologia estatística proposta pela American Society for Clinical Veterinary Pathology (ASCPV). Os IRs foram determinados por meio do programa Excel com o suplemento Reference Value Advisor (versão 2.1). O software realiza os cálculos de acordo com as recomendações CLSI, conforme sugerido pelas diretrizes da ASCVP. Um total de 25 parâmetros (13 hematológicos e 12 bioquímicos) foram submetidos ao processo de validação. Desse total, seis (24%) parâmetros não foram validados (Hemoglobina, CHGM, eosinófilos, linfócitos, albumina e GGT) e para estes foram estabelecidos novos intervalos. Para a maioria dos parâmetros hematológicos e bioquímicos (76%), os IRs estabelecidos previamente na literatura ainda são válidos para utilização em cães criados na Amazônia Oriental. Para os parâmetros não validados, apenas para eosinófilos e linfócitos recomendamos que outros trabalhos, com um número maior de animais, sejam realizados para confirmar os nossos resultados ou estabelecer novos IRs. Os IRs estabelecidos para hemoglobina, CHGM, albumina e GGT podem ser utilizados em substituição aos intervalos antigos.


The aim of the present study was to validate reference intervals (RIs) for hematology and blood biochemistry of dogs domiciled in the Eastern Amazon and to establish new RIs for parameters not yet validated. Samples from 44 clinically healthy adult dogs of different breeds and sexes were used. The methodology proposed by the Clinical and Laboratory Standards Institute (CLSI) was used for validation, and the statistical methodology proposed by the American Society for Clinical Veterinary Pathology (ASCPV) was used to determine the RIs of the non-validated parameters. The IRs were determined using the Excel program with the Reference Value Advisor add-in (version 2.1). The software performs calculations according to CLSI recommendations as suggested by the ASCVP guidelines. A total of 25 parameters (13 hematological and 12 biochemical) were submitted to the validation process. Of this total, six (24%) parameters were not validated (Hemoglobin, CHGM, eosinophils, lymphocytes, albumin and GGT) and new intervals were established for these. For most hematological and biochemical parameters (76%), the RIs previously established in the literature are still valid for use in dogs raised in the Eastern Amazon. For non-validated parameters, just for eosinophils and lymphocytes, we recommend that other studies, with a larger number of animals, be carried out to confirm our results or establish new IRs. The established IRs for hemoglobin, CHGM, albumin and GGT can be used in place of the old ranges.


Assuntos
Animais , Cães , Valores de Referência , Cães , Testes Hematológicos/veterinária , Bioquímica , Ecossistema Amazônico , Hematologia
10.
J Ethnopharmacol ; 144(2): 346-52, 2012 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-23000170

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: The lack of ethnoveterinary surveys in Brazil, especially in the Amazon region, results in losses in the veterinary phytopharmacology field and in scientific documentation of the cultural traditions of plant use in the treatment of animal diseases. AIM OF THE STUDY: To catalog, analyze and disseminate the ethnoveterinary knowledge of the inhabitants of Colares Island, Pará state, eastern Amazon, Brazil. MATERIALS AND METHODS: A total of 72 interviews were conducted, and semi-structured questionnaires were answered by 18 men and 54 women. The data obtained were quantitatively analyzed using the informant consensus factor (ICF) and use value (UV). The plants with a reported medicinal use for domestic animals were harvested, herbalized and botanically identified. RESULTS: Fifty-six plants, distributed in 49 genera and 35 families, were indicated to have 23 different medicinal uses, divided into six categories of use. The highest ICF (0.80) was obtained for the antiparasitic class. The Euphorbiaceae family exhibited the highest number of citations, and the species with the highest UVs were Caladium cf. bicolor, Bixa orellana, Carapa guianensis, Jatropha curcas and Cymbopogon citratus. The parts of the 56 plants that were most frequently used to prepare ethnoveterinary medications were the leaves (46%), bark (15%), roots and fruit (10%). The use of the macerated leaves was the most common method of application, used by 43% of the interviewees, and the majority of the preparations (87.3%) used a single plant. In addition to medicinal plants, the interviewees reported the use of products of animal and mineral origin. CONCLUSION: The present study contributed to the establishment of an inventory of plants used in ethnoveterinary practices in this region of the Brazilian eastern Amazon. Future phytochemical and pharmacological studies are needed to confirm the efficacy and safety of the identified plants, enabling communities to use them in a more economic, effective and safe manner.


Assuntos
Conhecimentos, Atitudes e Prática em Saúde , Fitoterapia/veterinária , Plantas Medicinais , Drogas Veterinárias , Adulto , Idoso , Animais , Brasil , Etnofarmacologia , Feminino , Humanos , Masculino , Medicina Tradicional , Pessoa de Meia-Idade , Inquéritos e Questionários
11.
Rev Bras Parasitol Vet ; 20(2): 155-60, 2011.
Artigo em Inglês | MEDLINE | ID: mdl-21722491

RESUMO

Tanniferous plants represent a promising alternative for controlling gastrointestinal nematodes of small ruminants. This experiment evaluated the effects of extracts from the leaf and stem of Anadenanthera colubrina, Leucaena leucocephala and Mimosa tenuiflora on larval exsheathment of Haemonchus contortus in vitro and verified the role of tannins in this process. Third-stage larvae of H. contortus were incubated with extracts for 3 hours and were exposed to sodium hypochlorite solution. The extracts were tested at 300 µg.mL(-1) and accompanied by controls: phosphate buffer solution (PBS) and polyvinyl polypyrrolidone (PVPP). The larval exsheathment was evaluated for 60 minutes, and the results were subjected to the Kruskal-Wallis test (p < 0.05). The six extracts blocked larval exsheathment. After PVPP addition, a tannin inhibitor, the exsheathment percentage was similar to the PBS (p > 0.05), except for L. leucocephala and M. tenuiflora leaf extracts. However, pre-incubation with PVPP of these two extracts significantly changed larval exsheathment when compared to the non-treated extracts (p < 0.05). These results suggest that A. colubrina, L. leucocephala and M. tenuiflora could be useful in gastrointestinal nematode control and that tannins are probably the main compounds involved in the observed effects. However, in vivo and toxicological studies should be conducted.


Assuntos
Haemonchus/efeitos dos fármacos , Extratos Vegetais/farmacologia , Taninos/farmacologia , Animais , Larva/efeitos dos fármacos
12.
Braz. j. vet. res. anim. sci ; 52(1): 34-40, abr. 2015. graf, tab
Artigo em Português | LILACS | ID: lil-786782

RESUMO

Libidibia ferrea é uma planta muito utilizada popularmente para fins terapêuticos, inclusive para acelerar processos de cicatrização de feridas cutâneas. O presente trabalho pesquisou a composição química e avaliou o potencial cicatrizante do extrato etanólico dos frutos de L. ferrea (Mart. ex Tul.) em ratos. Foram utilizados 24 ratos Wistar divididos em quatro grupos. De todos os animais, foi retirado um fragmento de pele do dorso e cada grupo recebeu um tratamento diferente: solução de NaCl 0,9%, digliconato de clorexidina 1%, extrato etanólico dos frutos de Libidibia ferrea 12,5% e 50%. O processo de cicatrização foi avaliado macro e microscopicamente. Para a cicatrização de pele em ratos o extrato etanólico dos frutos de L. ferrea a 12,5% é significativamente mais eficiente do que a 50%. Saponinas, ácidos orgânicos, açúcares redutores, fenóis e taninos, sesquiterpenolactonas e outras lactonas, e antraquinonas foram encontrados no extrato.


Libidibia ferrea is a plant popularly used for therapeutic purposes, including processes to accelerate wound healing. The present investigation analyzed the chemical composition and the healing potential of ethanolic extract of the fruits of L. ferrea (Mart. ex Tul.) in rats. This study used 24 Wistar rats divided into four groups. In all animals a piece of skin on the back was removed and each group received a different treatment: NaCl 0.9%, Chlorhexidine digluconate 1%, ethanol extract of the fruits of Libidibia ferrea 12.5% and 50%. The healing process was evaluated macroscopically and microscopically. The ethanolic extract of the fruits of L. ferrea 12.5% was significantly more efficient than the 50% healing in rat skin.


Assuntos
Animais , Caesalpinia/química , Cicatrização , Ratos Wistar/lesões , Regeneração , Extratos Vegetais/uso terapêutico , Fitoterapia/veterinária
13.
Acta amaz ; 45(3): 317-322, July-Sept. 2015. tab
Artigo em Português | LILACS, VETINDEX | ID: biblio-1455257

RESUMO

Blood count and biochemical tests are used to diagnose diseases in domestic animals. These tests can be influenced by age, gender, nutrition, breed, species and environmental conditions. Thus, data from one region should not be extrapolated to animals raised in other regions. The objective of this study was to determine the hematological and biochemical values of different ages and genders of Santa Inês sheep, raised in the eastern Amazon. There were examined 91 sheep that were assigned to three groups: G1 (three to six months old, n = 31); G2 (seven to 24 months old, n = 30) and G3 (above 24 months old, n = 30). The blood cell count, white blood count and biochemical determinations were made with an automatic counter and a semi-automatic analyzer, respectively. Mean values were compared using the Tukey test. The number of erythrocytes, the red blood indices, the thrombogram, eosinophils, total protein, urea and creatinine concentrations were influenced by the age of the animals. The erythrocyte coefficient of variation and the creatinine concentration were influenced by gender, and were greater in males. The neutrophil:lymphocyte (N:L) ratio was greater than one for all age groups. This study led to the determination of reference values for sheep raised in the Eastern Amazon and demonstrated that when interpreting hematological and biochemical tests of sheep, age and gender must be considered.


O hemograma e o perfil bioquímico são usados para diagnosticar doenças em animais domésticos. Esses exames podem ser influenciados pela idade, sexo, nutrição, raça, espécie e condições ambientais. Portanto, dados de uma região não podem ser totalmente extrapolados para animais criados em regiões geograficamente distintas. Isso se aplica a ovinos criados no Bioma Amazônico. O objetivo deste estudo foi determinar os valores hematológicos e bioquímicos de ovinos Santa Inês, de diferentes idades e gêneros criados na Amazônia oriental. Foram examinados 91 ovinos divididos em três grupos: G1 (3-6 meses de idade, n = 31); G2 (7 a 24 meses de idade, n = 30) e G3 (mais de 24 meses de idade, n = 30). O hemograma e as determinações bioquímicas foram realizados com um contador automático e um analisador semi-automático, respectivamente. Os resultados foram comparados pelo teste de Tukey. O número de eritrócitos, índices eritrocitários, plaquetograma, número de eosinófilos, teor de proteína total, de ureia e de creatinina foram influenciados pela idade dos animais. O coeficiente de variação dos eritrócitos e a concentração de creatinina foram influenciados pelo sexo, sendo maiores nos machos. A relação neutrófilos:linfócitos (N:L) foi maior que um para todos os grupos etários. Neste estudo foram determinados valores de referência para ovinos criados na Amazônia Oriental. Além disso, demonstrou-se que ao interpretar exames hematológicos e bioquímicos de ovinos, a idade e o sexo devem ser considerados.


Assuntos
Animais , Fatores Etários , Ovinos/crescimento & desenvolvimento , Ovinos/sangue , Contagem de Células Sanguíneas/veterinária , Contagem de Leucócitos/veterinária , Técnicas e Procedimentos Diagnósticos/veterinária , Valores de Referência
14.
Acta amaz ; 41(2): 233-242, 2011. tab
Artigo em Inglês | LILACS, VETINDEX | ID: lil-586478

RESUMO

There have been ethnoveterinary reports from around the world investigating plant usage in therapeutic protocols; however, there is no information regarding the ethnoveterinary practices in Brazilian Amazonia. The objective of this work was to register and document the ethnoveterinary knowledge of the inhabitants of the Island of Marajó, eastern Amazonia, Brazil. In the study, interviews were conducted with 50 individuals, with the application of semi-structured questionnaires that were quantitatively analyzed using descriptive statistic methods of frequency distribution. Use-value was calculated to determine the most important species. Samples of plants that were reported to have medicinal value were collected and identified by botanical classification. Fifty plants, distributed among 48 genera and 34 families, were indicated for 21 different medicinal uses. The family Asteraceae had the largest number of reported species; Carapa guianensis Aubl., Copaifera martii Hayne, Crescentia cujete L., Caesalpinia ferrea Mart., Chenopodium ambrosioides L., Jatropha curcas L. and Momordica charantia L. were species with highest use- value. The plant parts that were more commonly utilized for the preparation of ethnoveterinary medicines were the leaves (56 percent), bark (18 percent), roots (14 percent), seeds (14 percent) and fruit (8 percent). With regard to usage, tea was reported as a usage method by 56 percent of the informants; most preparations (90.9 percent) utilized only a single plant. In addition to medicinal plants, informants reported using products of animal and mineral origin. The present study contributed to the construction of an inventory of Marajó Island's ethnoveterinary plants, which might be the basis for future scientific validation studies.


Em várias partes do mundo existem relatos etnoveterinários sobre a utilização de plantas em protocolos terapêuticos, entretanto não existem informações disponíveis sobre a etnoveterinária praticada na Amazônia brasileira. Desta forma, objetivou-se documentar o conhecimento etnoveterinário de habitantes da Ilha do Marajó, Amazônia Oriental. Foram realizadas 50 entrevistas individuais com aplicação de questionários semi-estruturados que foram analisados quantitativamente através de estatística descritiva utilizando freqüência de distribuição. O valor de uso foi calculado para determinar as espécies mais importantes. Amostras de plantas com relatos de uso medicinal foram coletadas e identificadas botanicamente. Cinqüenta plantas, distribuídas em 48 gêneros e 34 famílias, foram indicadas para 21 diferentes usos medicinais. A família Asteraceae foi a que teve maior número de espécies citadas e Carapa guianensis Aubl, Crescentia cujete L., Copaifera martii Hayne, Caesalpinia ferrea Mart., Chenopodium ambrosioides L., Jatropha curcas L. e Momordica charantia L. foram as espécies com maiores valor de uso. As partes das plantas mais utilizadas para preparo dos medicamentos etnoveterinários foram folhas (56 por cento), cascas (18 por cento), raizes (14 por cento), sementes (14 por cento) e frutos (8 por cento). Quanto à forma de uso o chá foi citado por 56 por cento dos entrevistados e a maioria das preparações (90,9 por cento) utiliza uma só planta. Além das plantas medicinais, os entrevistados relataram o uso de produtos de origem animal e mineral. Esse trabalho contribui para realização de um inventário das plantas utilizadas na etnoveterinária marajoara que pode servir de base de dados para futuros estudos de validação científica.


Assuntos
Humanos , Plantas Medicinais , Medicina Veterinária , Ecossistema Amazônico , Etnobotânica
15.
Rev. bras. parasitol. vet ; 20(2): 155-160, Apr.-June 2011. graf, tab
Artigo em Inglês | LILACS | ID: lil-604698

RESUMO

Tanniferous plants represent a promising alternative for controlling gastrointestinal nematodes of small ruminants. This experiment evaluated the effects of extracts from the leaf and stem of Anadenanthera colubrina, Leucaena leucocephala and Mimosa tenuiflora on larval exsheathment of Haemonchus contortus in vitro and verified the role of tannins in this process. Third-stage larvae of H. contortus were incubated with extracts for 3 hours and were exposed to sodium hypochlorite solution. The extracts were tested at 300 µg.mL-1 and accompanied by controls: phosphate buffer solution (PBS) and polyvinyl polypyrrolidone (PVPP). The larval exsheathment was evaluated for 60 minutes, and the results were subjected to the Kruskal-Wallis test (p < 0.05). The six extracts blocked larval exsheathment. After PVPP addition, a tannin inhibitor, the exsheathment percentage was similar to the PBS (p > 0.05), except for L. leucocephala and M. tenuiflora leaf extracts. However, pre-incubation with PVPP of these two extracts significantly changed larval exsheathment when compared to the non-treated extracts (p < 0.05). These results suggest that A. colubrina, L. leucocephala and M. tenuiflora could be useful in gastrointestinal nematode control and that tannins are probably the main compounds involved in the observed effects. However, in vivo and toxicological studies should be conducted.


Plantas taniníferas representam uma promissora alternativa de controle dos nematóides gastrintestinais de pequenos ruminantes. Esse experimento avaliou in vitro os efeitos dos extratos das folhas e caules de Anadenanthera colubrina, Leucaena leucocephala e Mimosa tenuiflora sobre o desembainhamento larvar de Haemonchus contortus e verificou o papel dos taninos nesse processo. Larvas de terceiro estádio de H. contortus foram incubadas com 300 µg.mL-1 de extrato por 3 horas e expostas a uma solução de hipoclorito de sódio. O ensaio foi acompanhado por controles: solução salina tamponada com fosfato (PBS) e polivinilpolipirrolidona (PVPP). O desembainhamento larvar foi avaliado durante 60 minutos e os resultados submetidos ao teste Kruskal-Wallis (p < 0,05). Os seis extratos bloquearam o desembainhamento larvar. Após adição de PVPP, um inibidor de taninos, o percentual de desembainhamento foi similar ao PBS (p > 0,05), exceto nos extratos das folhas de L. leucocephala e M. tenuiflora. Entretanto, a pré-incubação desses dois extratos com PVPP alterou significativamente o desembainhamento quando comparado com extratos não-tratados (p < 0,05). Esses resultados sugerem que A. colubrina, L. leucocephala e M. tenuiflora podem ser úteis no controle de nematóides gastrintestinais e que os taninos são provavelmente os principais compostos envolvidos nos efeitos. Contudo, estudos toxicológicos e in vivo são necessários.


Assuntos
Animais , Haemonchus/efeitos dos fármacos , Extratos Vegetais/farmacologia , Taninos/farmacologia , Larva/efeitos dos fármacos
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