RESUMO
Magnetic field exposure was consistently found to affect pain inhibition (i.e. analgesia). Recently, we showed that an extreme reduction of the ambient magnetic and electric environment, by mu-metal shielding, also affected stress-induced analgesia (SIA) in C57 mice. Using CD1 mice, we report here the same findings from replication studies performed independently in Pisa, Italy and London, ON, Canada. Also, neither selective vector nulling of the static component of the ambient magnetic field with Helmholtz coils, nor copper shielding of only the ambient electric field, affected SIA in mice. We further show that a pre-stress exposure to the mu-metal box is necessary for the anti-analgesic effects to occur. The differential effects of the two near-zero magnetic conditions may depend on the elimination (obtained only by mu-metal shielding) of the extremely weak time-varying component of the magnetic environment. This would provide the first direct and repeatable evidence for a behavioural and physiological effect of very weak time-varying magnetic fields, suggesting the existence of a very sensitive magnetic discrimination in the endogenous mechanisms that underlie SIA. This has important implications for other reported effects of exposures to very weak magnetic fields and for the theoretical work that considers the mechanisms underlying the biological detection of weak magnetic fields.
Assuntos
Analgesia , Campos Eletromagnéticos , Animais , Canadá , Estimulação Elétrica , Feminino , Itália , Masculino , Camundongos , Medição da Dor , Estresse Fisiológico , Reino UnidoRESUMO
The toxicity of gadolinium (Gd) based MRI contrast agents, is based upon the amount of Gd that dissociates from its chelate and deposits in tissues. In this study, the toxicities of two contrast agents were tested using different injection strategies in two animal models. Following a bolus injection of 0.2 mmol/kg of Gd-DTPA in a pilot study with a single canine, Gd levels were as high as 2.05 +/- 0.17 ppm and 0.47 +/- 0.11 ppm 2 weeks post injection in the kidney and liver tissues, respectively. To evaluate the role that the injection strategy plays in toxicity, 0.8 mmol/kg of Gd-(HP-DO3A) was injected into rats, in a second study, via bolus and constant infusion techniques. Gd was only detected in the kidney in the bolus injected rats but in the lung as well in the constant infusion injected rats. Concentrations detected in the kidney for both strategies, were comparable within error: 1.37 +/- 0.46 ppm for the bolus and 1.24 +/- 0.39 ppm for the bolus/constant infusion strategy and 0.16 +/- 0.14 ppm in the lung for the constant infusion technique. The contrast infusion technique does not appear to present an increased risk of toxicity over the bolus technique except perhaps to a small degree in the lung.
Assuntos
Meios de Contraste/toxicidade , Gadolínio DTPA/toxicidade , Compostos Heterocíclicos/toxicidade , Imageamento por Ressonância Magnética , Compostos Organometálicos/toxicidade , Animais , Meios de Contraste/administração & dosagem , Cães , Gadolínio , Gadolínio DTPA/administração & dosagem , Compostos Heterocíclicos/administração & dosagem , Infusões Intravenosas , Injeções Intravenosas , Análise de Ativação de Nêutrons , Compostos Organometálicos/administração & dosagem , RatosRESUMO
Dielectric relaxation data covering a temperature range from above room temperature to below the glass transition for 40% (w/w) and 75% (w/w) glucose/water solutions in the frequency range between 5 and 13 MHz are presented. These data are used to obtain correlation times for the dielectric relaxation in the viscous liquid and the glass and are compared with correlation times determined from deuterium nuclear spin relaxation times [J. Chem. Phys., 110 (1999) 3472-3483]. The two sets of results have the same temperature dependence, but differ in magnitude by a factor of 3, implying that the relaxation is a small-step rotational diffusion. Both the structural relaxation (alpha process) and the slow beta process are present. In the 40% glucose/water sample, there is a dielectric relaxation attributable to the ice that forms at low temperature. It is shown that the reciprocal of the viscosity, the correlation time derived from the dielectric relaxation, and the dc conductivity have a similar dependence on temperature.
Assuntos
Glucose/química , Condutividade Elétrica , Eletroquímica , Géis , Soluções , Eletricidade Estática , Temperatura , ViscosidadeRESUMO
The tissue homogeneity model, which describes tissue in terms of two compartments, one intravascular (iv) and one extravascular (ev), is solved by Laplace transformation of two coupled differential equations. By assuming a functional form for the arterial input function (AIF), or by fitting to an experimentally determined AIF, this function is introduced into the solution as a boundary condition describing the time dependent input to the capillary. The solution to the tissue homogeneity model equations in Laplace space are numerically inverted to obtain the concentration of tracer in the ev space as a function of time and in the iv space as a function of both position and time. Magn Reson Med 45:42-45, 2001.
Assuntos
Simulação por Computador , Meios de Contraste/administração & dosagem , Meios de Contraste/farmacocinética , Imageamento por Ressonância Magnética , Modelos Biológicos , Injeções Intravenosas , Fluxo Sanguíneo RegionalRESUMO
A simulation is presented which calculates the MRI signal expected from a model tissue for a given pulse sequence after a bolus injection of a contrast agent. The calculation assumes two physiologic compartments only, the intravascular and extravascular spaces. The determination of the concentration of contrast in each compartment as a function of time and position has been outlined in a previous publication (Moran and Prato, Magn Reson Med 2001;45:42-45). These contrast agent concentrations are used here to determine the NMR relaxation times as a function of time and position within the tissue. Knowledge of this simulated tissue 'map' of relaxation times as a function of time provides the information required to determine whether the proton exchange rate is fast or slow on the NMR timescale. Since with a bolus injection the concentration of contrast and hence the relaxation time may vary with position along the capillary, some segments of the capillary are allowed to be in fast exchange with the extravascular space, while others may be in slow exchange. Using this information, and parameters specific to a given tissue, the MRI signal for a given pulse sequence is constructed which correctly accounts for differences in proton exchange across the length of the capillary. It is shown that extravascular contrast agents show less signal dependence on water exchange, and thus may be more appropriate for quantitative imaging when using fast exchange assumptions. It is also shown that nondistributed compartment models can incorrectly estimate the water exchange that is occurring at the capillary level if exchange-minimizing pulse sequences are not used.
Assuntos
Meios de Contraste , Hidrogênio/farmacocinética , Aumento da Imagem/métodos , Imageamento por Ressonância Magnética/métodos , Modelos Biológicos , Algoritmos , Água Corporal/metabolismo , Capilares/metabolismo , Permeabilidade Capilar , Simulação por Computador , Meios de Contraste/farmacocinética , Gadolínio DTPA/farmacocinética , Humanos , Espectroscopia de Ressonância Magnética , Força Próton-Motriz , Fatores de Tempo , Distribuição TecidualRESUMO
We report a PCR deletion mutagenesis method for the exact positioning of a foreign gene (pobA) in the lac operon of an expression plasmid in place of the lacZ protein code. This method requires the synthesis of four oligonucleotides and three PCR reactions to delete unwanted bases and retain the nucleotide sequence naturally found between the lac promoter and the protein code. The engineered plasmid results in the production of at least 40% of the cellular protein as the foreign polypeptide. In the example presented the expression of the protein is high even with a substantial difference in codon usage between the host (Escherichia coli) and a foreign gene from Pseudomonas aeruginosa. Some of the polypeptide produced has the ame properties as native protein and is easily purified. The remainder is present as insoluble inclusion bodies. This method of plasmid refinement may be applicable to the expression of many proteins.
Assuntos
4-Hidroxibenzoato-3-Mono-Oxigenase/biossíntese , Proteínas de Bactérias/biossíntese , Regulação Bacteriana da Expressão Gênica , Mutagênese , Reação em Cadeia da Polimerase , Proteínas Recombinantes de Fusão/biossíntese , Deleção de Sequência , 4-Hidroxibenzoato-3-Mono-Oxigenase/genética , Proteínas de Bactérias/genética , Sequência de Bases , Códon , Escherichia coli/genética , Genes Bacterianos , Genes Sintéticos , Vetores Genéticos , Óperon Lac , Dados de Sequência Molecular , Pseudomonas aeruginosa/genéticaRESUMO
A continuous fluorometric assay for tryptophan hydroxylase activity based on the different spectral characteristics of tryptophan and 5-hydroxytryptophan is presented. Hydroxylation of tryptophan at the 5-position results in a large increase in the fluorescence of the molecule. The assay selectively monitors the fluorescence yield of 5-hydroxytryptophan by exciting the reaction mix at 300 nm. The rate of increase of the emission signal was found to be directly proportional to the enzyme concentration. Inner filter effects due to quinonoid dihydropterin accumulation were eliminated by the inclusion of a thiol reductant. Activity measured using this assay method was found to be the same as that determined by established discontinuous HPLC assay methods. The application of the assay to routine activity measurements and to steady-state determinations with the substrates tryptophan and tetrahydropterin is described.
Assuntos
Espectrometria de Fluorescência/métodos , Triptofano Hidroxilase/análise , Triptofano Hidroxilase/metabolismo , 5-Hidroxitriptofano/química , 5-Hidroxitriptofano/metabolismo , Cinética , Pterinas/química , Pterinas/metabolismo , Reprodutibilidade dos Testes , Espectrometria de Fluorescência/normas , Triptofano/química , Triptofano/metabolismoRESUMO
Tryptophan hydroxylase is a pterin-dependent amino acid hydroxylase that catalyzes the incorporation of one atom of molecular oxygen into tryptophan to form 5-hydroxytryptophan. The substrate specificity and hydroxylation regiospecificity of tryptophan hydroxylase have been investigated using tryptophan analogues that have methyl substituents or nitrogens incorporated into the indole ring. The products of the reactions show that the regiospecificity of tryptophan hydroxylase is stringent. Hydroxylation does not occur at the 4 or 6 carbon in response to changes in substrate topology or atomic charge. 5-Hydroxymethyltryptophan and 5-hydroxy-4-methyltryptophan are the products from 5-methyltryptophan. These products establish that the hydroxylating intermediate is sufficiently potent to hydroxylate benzylic carbons and that the direction of the NIH shift in tryptophan hydroxylase is from carbon 5 to carbon 4. The effects on the V/K values for the amino acids indicate that the enzyme is most sensitive to changes at position 5 of the indole ring. The V(max) values for amino acid hydroxylation differ at most by a factor of 3 from that observed for tryptophan, while the efficiencies of hydroxylation with respect to tetrahydropterin consumption vary 6-fold, consistent with oxygen transfer to the amino acid being partially or fully rate limiting in productive catalysis.
Assuntos
Triptofano Hidroxilase/química , Triptofano/análogos & derivados , Triptofano/química , Hidroxilação , Cinética , Proteínas Recombinantes/química , Escatol/química , Eletricidade Estática , Estereoisomerismo , Relação Estrutura-Atividade , Especificidade por Substrato , Triptofano Hidroxilase/genéticaRESUMO
Wild type rabbit tryptophan hydroxylase (TRH) and two truncated mutant proteins have been expressed in Escherichia coli. The wild type protein was only expressed at low levels, whereas the mutant protein lacking the 101 amino-terminal regulatory domain was predominantly found in inclusion bodies. The protein that also lacked the carboxyl-terminal 28 amino acids, TRH102-416, was expressed as 30% of total cell protein. Analytical ultracentrifugation showed that TRH102-416 was predominantly a monomer in solution. The enzyme exhibited an absolute requirement for iron (ferrous or ferric) for activity and did not turn over in the presence of cobalt or copper. With either phenylalanine or tryptophan as substrate, stoichiometric formation of the 4a-hydroxypterin was found. Steady state kinetic parameters were determined with both of these amino acids using both tetrahydrobiopterin and 6-methyltetrahydropterin.
Assuntos
Triptofano Hidroxilase/metabolismo , Animais , Catálise , Cromatografia por Troca Iônica , Cinética , Metais/metabolismo , Pterinas/metabolismo , Coelhos , Proteínas Recombinantes/química , Proteínas Recombinantes/isolamento & purificação , Proteínas Recombinantes/metabolismo , Triptofano Hidroxilase/química , Triptofano Hidroxilase/isolamento & purificação , UltracentrifugaçãoRESUMO
A hybrid two-dimensional relaxometry (2DR) sequence was used to simultaneously measure both the spin-spin (R2) and spin-lattice relaxation rates (R1) of skeletal muscle in vivo. The 2DR sequence involved a 180 degrees inversion pulse followed by a variable delay time (30 values from 40 to 7000 ms); a projection presaturation (PP) scheme to localize a 16-ml cylindrical voxel; and a CPMG sequence (950 even echoes, effective echo spacing = 1.2 ms, equilibrium time = 12 s). The 2DR data were collected at 3.0 Tesla from the flexor digitorum profundus of eight healthy males, 26 +/- 2 years old. Analysis was performed with a 2D version of the non-negative least-squares algorithm and a one-way ANOVA. All subjects exhibited at least three spin-groups (R2 < 200 s(-1)), designated B, C, and D, with R2 values of 42.7, 26.5, and 8.1 s(-1), and fractional volumes of 52, 35, and 7%, respectively. The R1 values of B and C were similar, congruent with0.7 s(-1), but different from that of D (P < 0.001), which had an R1 of 1.0 s(-1). The results suggest that exchange between B and C ranges from 0.7-16.2 s(-1), while exchange between either of these spin-groups with D is slower. If the data are interpreted with a compartment model, in which spin-groups with short and long R2 values are attributed to extra- and intracellular fluid, respectively, the exchange of water across the cell membrane in living skeletal muscle is slow or intermediate relative to both R1 and R2.
Assuntos
Algoritmos , Imageamento por Ressonância Magnética/métodos , Músculo Esquelético/anatomia & histologia , Adulto , Humanos , Masculino , Músculo Esquelético/metabolismoRESUMO
Results of simulations are shown which illustrate how the concentration-time curves of an extravascular extracellular (EVEC) contrast agent, such as Gd-DTPA, vary in myocardial tissue. The simulations show that the variable permeability of dead myocytes within a recent myocardial infarction will significantly alter delayed enhancement patterns following a bolus injection, invariably reducing the sensitivity of this technique for the detection of permanently damaged tissue. It is further predicted that if the bolus injection is followed by a suitably selected constant infusion, the infarct size and infarct volume of distribution may be more accurately determined, even though the degree of enhancement of an infarcted region (with normal flow) above normal tissue is slightly higher for the bolus technique within the first 30 min following the injection. The degree of enhancement of an infarcted region (with normal flow) above normal tissue was comparable between the two techniques at the point in the constant infusion at which the volume of contrast injected was the same as in the bolus case, i.e., at approximately 30 min after the bolus injection. The constant infusion approach became superior thereafter as overall tissue concentrations became greater in both normal and infarcted tissue, and these concentrations remained more stable with the constant infusion approach. Preliminary experimental results in a canine model of infarction/reperfusion illustrated a delayed wash-in of contrast agent in infarcted tissue, which may be explained by a physiological model in which dead myocytes in infarcted myocardium have non-infinite permeability.
Assuntos
Meios de Contraste/metabolismo , Gadolínio DTPA , Imageamento por Ressonância Magnética , Modelos Cardiovasculares , Infarto do Miocárdio/metabolismo , Miocárdio/metabolismo , Animais , Meios de Contraste/administração & dosagem , Cães , Gadolínio DTPA/administração & dosagem , Gadolínio DTPA/metabolismo , Monitorização Fisiológica , Infarto do Miocárdio/diagnóstico , Infarto do Miocárdio/fisiopatologia , Projetos PilotoRESUMO
p-Hydroxybenzoate hydroxylase (EC 1.14.13.2) is a flavoprotein monooxygenase that catalyzes the incorporation of one atom of molecular oxygen into p-hydroxybenzoate to form 3,4-dihydroxybenzoate. The enzyme activates the substrate at the 3 position to electrophilic substitution by lowering the pKa of the phenolic oxygen. The results presented here indicate that regions of positive potential in the active site facilitate this substrate activation, which is necessary for rapid hydroxylation. We have neutralized a positive point charge by mutating lysine 297 to methionine (K297M). This mutation changes an amino acid near the active site, but not directly in contact with the flavin or the substrate. A variety of transient state kinetic and static parameters have been determined with two substrates. The results indicate that the K297M mutant does not activate the substrate through phenolic ionization to the same extent as wild-type (WT) and yet remains a competent hydroxylase. However, catalysis by the mutant is slow compared to that of WT, particularly in the oxidative half-reaction. Thus, normally quite labile oxygenated flavin intermediates encountered in the hydroxylation pathway of WT p-hydroxybenzoate hydroxylase are stabilized and their decay is rate limiting in the K297M turnover. Electrostatic potential calculations offer an explanation for the lack of substrate activation. The stability of the oxidative reaction intermediates seems to be related to a lower degree of substrate activation.
Assuntos
4-Hidroxibenzoato-3-Mono-Oxigenase/química , 4-Hidroxibenzoato-3-Mono-Oxigenase/metabolismo , Lisina , Metionina , Mutagênese , 4-Hidroxibenzoato-3-Mono-Oxigenase/genética , Sítios de Ligação , Catálise , Eletroquímica , Ativação Enzimática/efeitos dos fármacos , Hidroxilação , Cinética , Modelos Moleculares , Estrutura Molecular , Parabenos/metabolismo , Relação Estrutura-AtividadeRESUMO
The isoalloxazine ring system of the FAD cofactor of p-hydroxybenzoate hydroxylase must be secluded from solvent at specific stages of catalysis in order to form and stabilize a flavin C4a-hydroperoxide. This species may then react with the activated phenolate of p-hydroxybenzoate. A number of crystal structures of the enzyme with alterations to active site substituents or complexes with analogue benzoates have revealed an alternate position for the isoalloxazine (Gatti et al. (1994) Science 266, 110-114; Schreuder et al. (1994) Biochemistry 33, 10161-10170). This new flavin conformation is 7 A "out" toward solvent and may open a passage for substrate entry to the active site. Arginine 220 is one of the few residues in the structure to demonstrate conformational changes when the flavin is "out". In this study we have made the Arg220Lys mutant to test the significance of this residue in flavin movement. The R220K mutation has brought about dramatic alterations to all aspects of catalysis. Stopped flow kinetic characterization of the mutant has revealed that, while the effector role for the substrate is maintained, there exists an order of magnitude decrease in the limiting rate of reduction, even though there is 40-fold increase in association with NADPH. The mutant enzyme has only a fraction of its reductive half-reaction coupled to product formation, and the hydroxylation process is slow. This occurs despite a higher proportion of the more activated substrate phenolate in the active site. Many of the observed changes can be attributed to a decrease in the stability of the "in" conformation of the flavin during the catalysis and indicate a role for flavin conformational states in many of the catalytic processes of the enzyme.
Assuntos
4-Hidroxibenzoato-3-Mono-Oxigenase/química , 4-Hidroxibenzoato-3-Mono-Oxigenase/metabolismo , Flavinas/metabolismo , 4-Hidroxibenzoato-3-Mono-Oxigenase/genética , Arginina/metabolismo , Sítios de Ligação , Catálise , Flavina-Adenina Dinucleotídeo/metabolismo , Flavinas/química , Concentração de Íons de Hidrogênio , Cinética , Lisina/metabolismo , Modelos Moleculares , Estrutura Molecular , Mutagênese Sítio-Dirigida , Mutação , NADP/metabolismo , Oxirredução , Parabenos/metabolismo , Conformação Proteica , EspectrofotometriaRESUMO
The flavin of p-hydroxybenzoate hydroxylase (PHBH) adopts two conformations [Gatti, D. L., Palfey, B. A., Lah, M.-S., Entsch, B., Massey, V., Ballou, D. P., and Ludwig, M. L. (1994) Science 266, 110-114; Schreuder, H. A., Mattevi, A., Obmolova, G., Kalk, K. H., Hol, W. G. J., van der Bolt, F. J. T., and van Berkel, W. J. H. (1994) Biochemistry 33, 10161-10170]. Kinetic studies detected the movement of the flavin from the buried conformation to the exposed conformation caused by the binding of NADPH prior to its reaction with the flavin. The pH dependence of the rate constant for flavin reduction in wild-type PHBH and the His72Asn mutant indicates that the deprotonation of bound p-hydroxybenzoate is also required for flavin movement, and is accomplished by the same internal proton transport network previously found to be involved in substrate oxidation. The linkage of substrate deprotonation to flavin movement constitutes a novel mode of molecular recognition in which the enzyme tests the suitability of aromatic substrates before committing to the catalytic cycle.
Assuntos
4-Hidroxibenzoato-3-Mono-Oxigenase/química , 4-Hidroxibenzoato-3-Mono-Oxigenase/metabolismo , Conformação Proteica , Substituição de Aminoácidos , Sítios de Ligação , Flavina-Adenina Dinucleotídeo/metabolismo , Concentração de Íons de Hidrogênio , Cinética , Modelos Moleculares , Mutagênese Sítio-Dirigida , NAD/metabolismo , Pseudomonas aeruginosa/enzimologia , Pseudomonas aeruginosa/genética , Proteínas Recombinantes/química , Proteínas Recombinantes/metabolismoRESUMO
In the crystal structure of native p-hydroxybenzoate hydroxylase, Ser212 is within hydrogen bonding distance (2.7 A) of one of the carboxylic oxygens of p-hydroxybenzoate. In this study, we have mutated residue 212 to alanine to study the importance of the serine hydrogen bond to enzyme function. Comparisons between mutant and wild type (WT) enzymes with the natural substrate p-hydroxybenzoate showed that this residue contributes to substrate binding. The dissociation constant for this substrate is 1 order of magnitude higher than that of WT, but the catalytic process is otherwise unchanged. When the alternate substrate, 2,4-dihydroxybenzoate, is used, two products are formed (2,3,4-trihydroxybenzoate and 2,4, 5-trihydroxybenzoate), which demonstrates that this substrate can be bound in two orientations. Kinetic studies provide evidence that the intermediate with a high extinction coefficient previously observed in the oxidative half-reaction of the WT enzyme with this substrate is composed of contributions from both the dienone form of the product and the C4a-hydroxyflavin. During the reduction of the enzyme-2,4-dihydroxybenzoate complex by NADPH with 2, 4-dihydroxybenzoate, a rapid transient increase in flavin absorbance is observed prior to hydride transfer from NADPH to FAD. This is direct evidence for movement of the flavin before reduction occurs.