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Models help to describe and predict complex processes and scenarios that are difficult to understand or measure in environmental management systems. Thus, model simulations were performed (i) to calibrate HYDRUS-2D for water and solute movement as a possible decision support system for Candler and Immokalee fine sand using data from microsprinkler and drip irrigation methods, (ii) to validate the performance of HYDRUS-2D using field data of microsprinkler and drip irrigation methods, and (iii) to investigate Br, NO, and water movement using annual or seasonal weather data and variable fertigation scenarios. The model showed reasonably good agreement between measured and simulated values for soil water content ( = 0.87-1.00), Br ( = 0.63-0.96), NO-N ( = 0.66-0.98), P ( = 0.25-0.78), and K ( = 0.44-0.99) movement. The model could be successfully used for scheduling irrigation and predicting nutrient leaching for both microsprinkler and drip irrigation systems on Florida's sandy soils.
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Monitoramento Ambiental/métodos , Modelos Químicos , Nitrogênio/análise , Fósforo/análise , Poluentes do Solo/análise , Movimentos da Água , Poluentes Químicos da Água/análise , Irrigação Agrícola , Agricultura/estatística & dados numéricos , Fertilizantes , Florida , SoloRESUMO
A procedure was developed to extract Imidacloprid (IMD) from newly-flushed and fully-expanded citrus leaves. The extraction was conducted in a bullet blender, using a small sample mass (0.5 g of fresh tissue), stainless-steel beads (24 g), and methanol as extractant (10 mL). The extracts did not require further clean-up before analysis by HPLC-MS/MS. The method was validated with control samples from IMD-untreated Hamlin orange trees. The method limit of detection and limit of quantitation were 0.04 and 0.12 µg g(-1), respectively. IMD recoveries from fortified leaf tissue were between 92 % and 102 %, with relative standard deviations of <8 %. The method was further evaluated by extracting leaves from Hamlin orange trees treated with IMD. The treated trees showed maximum concentrations of 10.8 and 21.8 µg g(-1), observed at 20 days after applying two soil-drenching rates (0.51 and 1.02 kg IMD ha(-1)), respectively. This extraction technique will generate useful data on IMD plant uptake, foliar concentration, and correlations with Asian citrus psyllid (ACP) mortality or control. The method could be used to generate baseline data to improve IMD soil-drenching applications as the main management practice to control the ACP.
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Citrus , Imidazóis/análise , Inseticidas/análise , Nitrocompostos/análise , Folhas de Planta/química , Cromatografia Líquida de Alta Pressão , Imidazóis/química , Inseticidas/química , Neonicotinoides , Nitrocompostos/química , Espectrometria de Massas em TandemRESUMO
Flooding is one of the major abiotic stresses for vegetable production in Florida. Hydroponic and pot trials were conducted with snap bean to evaluate the effects of oxygen fertilization on the biochemical and physiological status of flooded snap bean plants. There were three treatments in the hydroponic trials were: (1) flooded (control), (2) bubble aeration with ambient air, and (3) hydrogen peroxide (H2O2) applied at the beginning of the trial. Plant health was evaluated by determining nitrogen (N) and phosphorus (P) uptake rates. The greenhouse pot trials were used to quantify the effects of three different application rates of solid oxygen fertilizers as calcium peroxide (CaO2) and magnesium peroxide (MgO2). The results showed that plant N and P uptake rates were significantly greater (p < 0.05) with H2O2 than without H2O2. The N uptake rates with H2O2 were like that of those with bubbling. The uptake rate of NH4+ was significantly greater than that of NO3- with the bubbling and H2O2 conditions, but the uptake rate of NO3- was significantly greater than that of NH4+ in the flooding condition. The plant height, leaf greenness, shoot biomass, and yield were all significantly greater with CaO2 or MgO2 than without either solid oxygen fertilizer. The minimum damage of flooded snap bean was found with 2 g CaO2 or 4 g MgO2 per pot. These results indicated that oxygen fertilization may potentially improve yield of flooded snap bean plants.
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Phaseolus , Fertilização , Fertilizantes , Peróxido de Hidrogênio , Óxido de Magnésio , Oxigênio , Phaseolus/fisiologiaRESUMO
Citrus greening (huanglonbing (HLB)) disease has drastically reduced citrus fruit production in Florida over the last two decades. Scientists have developed sustainable nutrient management practices to live with the disease and continue fruit production. Best pesticide management practices have been devised to reduce the spread of HLB by Asian citrus psyllid (ACP). However, soil application of excessive nutrients and the use of soil drench application of pesticides to huanglongbing-infected citrus groves have been a serious environmental concern since the recent development of resistance to some pesticides. It is important to understand the consequences of applying pesticides and nutrients beyond the recommended application rates with an inappropriate method for citrus growth and development. Alternatively, foliar sprays of some nutrients proved effective to meet plants' requirements, and foliar sprays of effective insecticide products could provide an adequate mode of action for group rotation to minimize insecticide resistance by ACP and other pests. Sustainability in citrus production systems should include best management practices that improve pesticide and nutrient efficiency by including the total maximum daily load exiting the grove to reduce pesticide and nutrient exports into waterbodies.
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The fate of foliar and ground-applied essential nutrients is the least studied topic under citrus greening or Huanglongbing (HLB)-affected citrus, which is inherently suffering from severe root decline because of HLB-associated problems. The objective of this study was to evaluate if ground-applied coupled with foliar spray of essential nutrients can reverse the decline in tree growth and understand the fate of the nutrients in the soil-root-tree interfaces. The treatments were arranged in a split-split plot design in which nitrogen (N) was ground-applied in 20 splits biweekly and Mn, Zn, and B were foliar and /or ground-applied in three splits following the spring, summer, and late summer flush seasons. Soil nutrients in three depths (0-15, 15-30, and 30-45 cm), root, and leaf nutrient concentrations of the essential nutrients, leaf area index (LAI), and tree canopy volume (TCV) data were studied twice (spring and summer) for two years. A significantly higher soil NH4-N and NO3-N concentrations were detected in the topsoil depth than the two lower soil depths (15-30 and 30-45 cm) indicating lesser nutrient leaching as trees received moderate (224 kg ha-1) N rate. Except for soil zinc (Zn) concentration, all the nutrient concentrations were significantly higher in the topsoil (0-15 cm), compared with two lower soil depths indicating that Zn was intricate by changes in soil environmental conditions, root acquisition, and/or leaching to lower soil depth. Leaf N concentration significantly increased over time following seasonal environmental fluctuations, tree growth, and development. Thus, leaf N concentration remained above the optimum nutrient range implying lower N requirement under irrigation scheduling with SmartIrrigation, an App used to determine the daily irrigation duration to meet tree water requirement and split fertigation techniques. Root Manganese (Mn) and Zn concentrations were significantly higher in the root tissues of the treated than the control trees and translocated to the leaves accordingly. Meanwhile, a significantly higher LAI for trees budded on Swingle (Swc) rootstock however, larger TCV for trees budded on Volkameriana (Volk) rootstocks. The trees had significantly larger TCV when the trees received a moderate N rate during early study years and under foliar 9 kg ha-1 coupled with the ground 9 kg ha-1 Mn and Zn treatments during the late study years. Therefore, split ground application of 224 kg ha-1 of N, foliar applied 9 kg ha-1 coupled with ground-applied 9 kg ha-1 Mn and Zn were the suggested rates to sustain the essential leaf nutrient concentration within the optimum ranges and improve the deterioration of vegetative growth associated with HLB-induced problems of citrus trees.
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Water flow and P dynamics in a low-relief landscape manipulated by extensive canal and ditch drainage systems were modeled utilizing an ontology-based simulation model. In the model, soil water flux and processes between three soil inorganic P pools (labile, active, and stable) and organic P are represented as database objects. And user-defined relationships among objects are used to automatically generate computer code (Java) for running the simulation of discharge and P loads. Our objectives were to develop ontology-based descriptions of soil P dynamics within sugarcane- (Saccharum officinarum L.) grown farm basins of the Everglades Agricultural Area (EAA) and to calibrate and validate such processes with water quality monitoring data collected at one farm basin (1244 ha). In the calibration phase (water year [WY] 99-00), observed discharge totaled 11,114 m3 ha(-1) and dissolved P 0.23 kg P ha(-1); and in the validation phase (WY 02-03), discharge was 10,397 m3 ha(-1) and dissolved P 0.11 kg P ha(-). During WY 99-00 the root mean square error (RMSE) for monthly discharge was 188 m3 ha(-1) and for monthly dissolved P 0.0077 kg P ha(-1); whereas during WY 02-03 the RMSE for monthly discharge was 195 m3 ha(-1) and monthly dissolved P 0.0022 kg P ha(-1). These results were confirmed by Nash-Sutcliffe Coefficient of 0.69 (calibration) and 0.81 (validation) comparing measured and simulated P loads. The good model performance suggests that our model has promise to simulate P dynamics, which may be useful as a management tool to reduce P loads in other similar low-relief areas.
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Fósforo/análise , Saccharum/química , FloridaRESUMO
Citrus yields have declined by almost 56% since Huanglongbing (HLB) was first found in Florida (2005). That reduction forced citrus growers to replant trees at much higher densities to counter-balance tree loss. The current project aims to determine how much water is required to grow citrus trees at higher planting densities without reducing their productivity. The study was initiated in November 2017 on eight-month-old sweet orange (Citrus sinensis) trees grafted on the 'US-897' (Cleopatra mandarin × Flying Dragon trifoliate orange) citrus rootstock planted in the University of Florida, Southwest Florida Research and Education Center (SWFREC) demonstration grove, in Immokalee, FL (lat. 26.42° N, long. 81.42° W). The soil in the grove is Immokalee fine sand (Sandy, siliceous, hyperthermic Arenic Alaquods). The demonstration grove included three densities on two rows of beds (447, 598, and 745 trees per ha) replicated four times each and three densities of three rows of beds (512, 717, 897 trees per ha) replicated six times. Each density treatment was irrigated at one of two irrigation rates (62% or 100%) during the first 15 months (2017-2019) then adjusted (2019-2020) to represent 26.5, 40.5, 53, and 81% based on recommended young citrus trees evapotranspiration (ETc). Tree growth measurements including trunk diameter, height, canopy volume, leaf area, and root development were evaluated. During the first year, reducing the irrigation rate from 100% to 62% ETc did not significantly reduce the young citrus tree growth. Conversely, the lower irrigation rate (62% ETc) had increased citrus tree's leaf area, canopy volume and tree heights, root lifespan, and root length by 4, 9, 1, 2, and 24% compared with the higher irrigation rate (100%), respectively. Furthermore, the root lifespan was promoted by increasing planting density. For instance, the average root lifespan increased by 12% when planting density increased from 447 to 897 trees per ha, indicating that planting young trees much closer to each other enhanced the root's longevity. However, when treatments were adjusted from April 2019 through June 2020, results changed. Increasing the irrigation rate from 26.5% to 81% ETc significantly enhanced the young citrus tree growth by increasing citrus tree's canopy volume (four fold), tree heights (29%), root lifespan (86%), and root length (two fold), respectively. Thus, the application of 81% ETc irrigation rate in commercial citrus groves is more efficient for trees from two to four years of age.
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Florida ranks first among US states in fresh-market tomato production with annual production exceeding one-third of the total annual production in the country. Although tomato is a signature crop in Florida, current and future ambient temperatures could impose a major production challenge, especially during the fall growing season. This problem is increasingly becoming an important concern among tomato growers in south Florida, but studies addressing these concerns have not been conducted until now. Therefore, this study was conducted to determine the impacts of the present ambient temperature conditions and planting dates on tomato productivity in south Florida. The study was conducted using crop simulation model CROPGRO-Tomato of DSSAT (Decision Support System for Agricultural Transfer) version 4.7. Five treatments were evaluated, and included AT (simulated treatment using 14 years of actual daily weather conditions at the study location) while other treatments were conducted based on a percentage (-20%, -10%, +10%, +20%) of AT to simulate cooler and warmer temperature regimes. The results suggested that under the current temperature conditions during the fall growing season in south Florida, average tomato yield was up to 29% lower compared to the cooler temperature regimes. Tomato yield further decreased by 52% to 85% at air temperatures above the current condition. Yield reduction under high temperature was primarily due to lower fruit production. Contrary to yield, both tomato biomass accumulation and leaf area index increased with increase in temperature. Results also indicated that due to changes in air temperature pattern, tomato yield increased as planting date increased from July to December. Therefore, planting date modification during the fall season from the current July-September to dates between November and December will reduce the impacts of heat stress and increase tomato productivity in south Florida.
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Understanding citrus tree root development and dynamics are critical in determining crop best nutrient management practices. The role of calcium (Ca) and magnesium (Mg), manganese (Mn), Zinc (Zn), and boron (B) on huanglongbing (HLB) affected citrus trees' root growth and lifespan in Florida is not fully documented. Thus, the objective of this study was to determine the impact of foliar and ground-applied essential nutrients on seasonal fine root length density (FRLD; diameter (d) < 2 mm) and coarse roots (d > 2 mm), FRLD dynamics, root survival probability (lifespan), and root-zone soil pH of HLB-affected sweet orange trees. Results indicated that Ca treated trees budded on Cleopatra (Cleo) and Ca and Mg combined treatments on Swingle (Swc) rootstocks significantly increased seasonal FRLD of fine (< 2 mm) and coarse roots. The highest median root lifespan of Ca treated trees was 325 and 339 days for trees budded on Cleo and Swc rootstocks, respectively. In the second study, the coarse roots showed a significantly higher reaction to the nutrition applied than the fine roots. Meanwhile, the 2× (1× foliar and 1× ground-applied) treated trees showed a significantly higher median root lifespan compared to the other treatments. Thus, the current study unwraps future studies highlighting the combined soil and/or foliar application of the above nutrients to stimulate FRLD and improve root lifespan on HLB-affected sweet oranges with emphasis on root-zone soil pH.
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The following study was conducted to determine the impact of frequent foliar Cu applications on water relations of Huanglongbing (HLB)-affected Citrus sinensis cv. 'Valencia'. HLB in Florida is putatively caused by Candidatus Liberibacter asiaticus that is vectored by the Asian citrus psyllid. The experiment was conducted in a psyllid-free greenhouse with trees grown in Immokalee fine sand soil with the trees well-maintained to promote health. Cu was applied to the foliage at 0×, 0.5×, 1×, and 2× the commercially recommended rates, which were 0, 46, 92, and 184 mM, respectively, with applications made 3× in both 2016 and 2017. Previous studies indicate that HLB causes roots to decline before the canopy develops symptoms, which increases the ratio between the evaporative surface area of the canopy to the uptake surface area of roots and increases the hydraulic strain within the tree. In the current study, overall growth was suppressed substantially by HLB and Cu treatments but the ratio between evaporative surface area (leaf surface area) and the uptake surface area of roots (feeder root surface area) was not affected by either treatment. Stem water potential (Ψxylem), which was used as a measure of plant water deficits and the hydraulic strain within the tree, was significantly 13% lower for HLB-affected trees than the non-HLB controls but were not affected by Cu treatments. All Ψxylem measurements were in a range typical of well-watered trees conditions. Stomatal conductance (ks) and root and soil resistances (Rr+s) were not affected by HLB and Cu. The results of this experiment suggest that tree leaf area and feeder roots are reduced when the trees are affected by HLB or are treated with foliar Cu applications such that plant water deficits are not significantly different over that of the controls.
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Imidacloprid (IM) is used to control the Asian Citrus Psyllid (ACP) and citrus leafminer (CLM), which are related to the spread of huanglongbing (HLB or citrus greening) and citrus canker diseases, respectively. In Florida citrus, imidacloprid is mainly soil-drenched around the trees for proper root uptake and translocation into plant canopy to impact ACP and CLM. The objective of this study was to determine the effect of imidacloprid rate, and irrigate amount on concentration of imidacloprid in the soil following drench application to citrus trees in three age classes. The plots were established at the Southwest Florida Research and Education Center, Immokalee, using a randomized complete-block design for three age classes of trees: one-year-old trees (B1), three to five-year-old trees (B2), and eight-year-old trees (B3). The treatments were a combination of two rates each of imidacloprid (1D, 2D) and micro-sprinkling irrigation (1I, 2I). Imidacloprid and bromide (Br-) used as tracer were applied simultaneously. Soil moisture and concentrations of imidacloprid and Br were monitored using soil cores from hand held augers. Soil moisture content (θV) did not differ under two irrigation rates at any given observation day or depth, except following heavy rainfall events. Br- was lost from the observation depths (0-45 cm) about two weeks after soil-drench. Contrarily, imidacloprid persisted for a much longer time (4-8 weeks) at all soil depths, regardless of treatment combinations. The higher retardation of imidacloprid was related to the predominantly unsaturated conditions of the soil (which in turn reduced soil hydraulic conductivities by orders of magnitude), the imidacloprid sorption on soil organic matter, and the citrus root uptake. Findings of this study are important for citrus growers coping with the citrus greening and citrus canker diseases because they suggest that imidacloprid soil drenches can still be an effective control measure of ACP and CLM, and the potential for imidacloprid leaching to groundwater is minimal.
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Irrigação Agrícola/métodos , Citrus/parasitologia , Hemípteros/fisiologia , Lepidópteros/fisiologia , Neonicotinoides/metabolismo , Nitrocompostos/metabolismo , Solo/química , Irrigação Agrícola/instrumentação , Agricultura/métodos , Animais , Citrus/crescimento & desenvolvimento , Florida , Interações Hospedeiro-Parasita/efeitos dos fármacos , Inseticidas/farmacologia , Neonicotinoides/farmacologia , Nitrocompostos/farmacologia , Doenças das Plantas/parasitologia , Doenças das Plantas/prevenção & controle , Raízes de Plantas/crescimento & desenvolvimento , Raízes de Plantas/parasitologia , Reprodutibilidade dos TestesRESUMO
Imidacloprid (IMD) is a neonicotinoid pesticide soil-drenched to many crops to control piercing-sucking insects such as the Asian citrus psyllid (ACP). Neonicotinoids are persistent in the environment and transport analyses are helpful estimate leaching potential from soils that could result in groundwater pollution. The objective of this study was to analyze IMD breakthrough under saturated water flow in soil columns packed with three horizons (A, E, Bh) of Immokalee Fine Sand (IFS). Also, we used the dimensionless form of the convective-dispersive model (CD-Model) to compare the optimized transport parameters from each column experiment (retardation factor, R; fraction of instantaneous-to-total retardation, ß; and mass transfer coefficient, ω) with the parameters obtained from sorption batch equilibria and sorption kinetics. The tracer (Cl-) breakthrough curves (BTCs) were symmetrical and properly described by the CD-Model. IMD BTCs from A, Bh, and multilayered [A+E+Bh] soil columns showed steep fronts and tailing that were well described by the one-site nonequilibrium (OSNE) model, which was an evidence of non-ideal transport due to IMD mass transfer into the soil organic matter. In general, IMD was weakly-sorbed in the A and Bh horizons (R values of 3.72 ± 0.04 and 3.08 ± 0.07, respectively), and almost no retardation was observed in the E horizon (R = 1.20 ± 0.02) due to its low organic matter content (0.3%). Using the HYDRUS-1D package, optimized parameters (R, ß, ω) from the individual columns successfully simulated IMD transport in a multilayered column mimicking an IFS soil profile. These column studies and corresponding simulations agreed with previous findings from batch sorption equilibria and kinetics experiments, where IMD showed one-site kinetic mass transfer between soil surfaces and soil solution. Ideally, sandy soils should be maintained unsaturated by crop irrigation systems and rainfall monitoring during and after soil-drench application. The unsaturated soil will increase IMD retardation factors and residence time for plant uptake, lowering leaching potential from soil layers with low sorption capacity, such as the E horizon.
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Imidazóis/química , Inseticidas/química , Nitrocompostos/química , Poluentes do Solo/química , Solo/química , Poluentes Químicos da Água/química , Cinética , Modelos Teóricos , Neonicotinoides , Movimentos da ÁguaRESUMO
Nutrient assessment and management are important to maintain productivity in citrus orchards. In this study, laser-induced breakdown spectroscopy (LIBS) was applied for rapid and real-time detection of citrus anomalies. Laser-induced breakdown spectroscopy spectra were collected from citrus leaves with anomalies such as diseases (Huanglongbing, citrus canker) and nutrient deficiencies (iron, manganese, magnesium, zinc), and compared with those of healthy leaves. Baseline correction, wavelet multivariate denoising, and normalization techniques were applied to the LIBS spectra before analysis. After spectral pre-processing, features were extracted using principal component analysis and classified using two models, quadratic discriminant analysis and support vector machine (SVM). The SVM resulted in a high average classification accuracy of 97.5%, with high average canker classification accuracy (96.5%). LIBS peak analysis indicated that high intensities at 229.7, 247.9, 280.3, 393.5, 397.0, and 769.8 nm were observed of 11 peaks found in all the samples. Future studies using controlled experiments with variable nutrient applications are required for quantification of foliar nutrients by using LIBS-based sensing.
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Imidacloprid (IMD) is a neonicotinoid insecticide soil-drenched on sandy soils of southwest Florida for the control of Diaphorina citri Kuwayama or Asian citrus psyllid (ACP). The ACP vectors causal pathogens of a devastating citrus disease called citrus greening. Understanding the behavior of IMD in these soils and plants is critical to its performance against target pests. Samples from Immokalee fine sand (IFS) were used for sorption kinetics and equilibria experiments. IMD kinetics data were described by the one-site mass transfer (OSMT) model and reached equilibrium between 6 and 12 h. Batch equilibrium and degradation studies revealed that IMD was weakly sorbed (K(OC) = 163-230) and persistent, with a half-life of 1.0-2.6 years. Consequently, IMD has the potential to leach below the citrus root zone after the soil-drench applications.