RESUMO
The study of the neural basis of predatory behavior has been largely neglected over the recent years. Using an ethologically based approach, we presently delineate the prosencephalic systems mobilized during predation by examining Fos immunoreactivity in rats performing insect hunting. These results were further compared with those obtained from animals killed after the early nocturnal surge of food ingestion. First, predatory behavior was associated with a distinct Fos up-regulation in the ventrolateral caudoputamen at intermediate rostro-caudal levels, suggesting a possible candidate to organize the stereotyped sequence of actions seen during insect hunting. Insect predation also presented conspicuous mobilization of a neural network formed by a distinct amygdalar circuit (i.e. the postpiriform-transition area, the anterior part of cortical nucleus, anterior part of basomedial nucleus, posterior part of basolateral nucleus, and medial part of central nucleus) and affiliated sites in the bed nuclei of the stria terminalis (i.e. the rhomboid nucleus) and in the hypothalamus (i.e. the parasubthalamic nucleus). Accordingly, this network is likely to encode prey-related motivational values, such as prey's odor and taste, and to influence autonomic and motor control accompanying predatory eating. Notably, regular food intake was also associated with a relatively weak Fos up-regulation in this network. However, during regular surge of food intake, we observed a much larger mobilization in hypothalamic sites related to the homeostatic control of eating, namely, the arcuate nucleus and autonomic parts of the paraventricular nucleus. Overall, the present findings suggest potential neural systems involved in integrating prey-related motivational values and in organizing the stereotyped sequences of action seen during predation. Moreover, the comparison with regular food intake contrasts putative neural mechanisms controlling predatory related eating vs. regular food intake.
Assuntos
Comportamento Alimentar/fisiologia , Rede Nervosa/fisiologia , Vias Neurais/fisiologia , Comportamento Predatório/fisiologia , Prosencéfalo/fisiologia , Tonsila do Cerebelo/anatomia & histologia , Tonsila do Cerebelo/fisiologia , Animais , Mapeamento Encefálico , Ingestão de Alimentos/fisiologia , Hipotálamo/anatomia & histologia , Hipotálamo/fisiologia , Imuno-Histoquímica , Masculino , Modelos Neurológicos , Neostriado/anatomia & histologia , Neostriado/fisiologia , Rede Nervosa/anatomia & histologia , Vias Neurais/anatomia & histologia , Prosencéfalo/anatomia & histologia , Proteínas Proto-Oncogênicas c-fos/metabolismo , Ratos , Ratos Wistar , Núcleos Septais/anatomia & histologia , Núcleos Septais/fisiologia , Regulação para Cima/fisiologiaRESUMO
The efferent concentrations of the olfactory bulb (OB) in the opossum (Didelphis marsupialis aurita) were studied by the aid of the Fink-Heimer technique. Following lesions restricted to the OB, ipsilateral degenerating fibers entered the lateral olfactory tract and were treated to terminal fields essentially limited to the outer portion of the plexiform layer (sublamina IA) of the following structures: all the subdivisions of the anterior olfactory nucleus, the rostroventral tenia tecta, the full extent of the olfactory tubercle, the nucleus of the lateral olfactory tract, the anterior portion of the medial amygdaloid nucleus, the whole cortical amygdaloid nucleus (in the posteromedial subdivision of this structure the degeneration was very scanty), and the sulcal, piriform, and lateral entorhinal cortices. Some degree of topographical organization in the OB projections was noticed in the rostral portion of the lateral olfactory tract and within the external and lateral subdivisions of the anterior olfactory nucleus. In another series of experiments, when the lesion also involved the accessory olfactory bulb, heavy terminal degeneration occurred along the whole extent of the medial amygdaloid nucleus and in the posteromedial subdivision of the cortical amygdaloid nucleus. These findings indicate that, although very similar to those described in other mammals, the OB efferent connections in the opossum present some peculiarities; namely, the existence of dense terminal fields in the sulcal cortex and in the rostral district of the medial amygdaloid nucleus.
Assuntos
Bulbo Olfatório/anatomia & histologia , Gambás/anatomia & histologia , Tonsila do Cerebelo/anatomia & histologia , Animais , Dominância Cerebral/fisiologia , Vias Eferentes/anatomia & histologia , Feminino , Masculino , Degeneração Neural , Fibras Nervosas/ultraestrutura , Condutos Olfatórios/anatomia & histologiaRESUMO
Restricted microelectrophoretic injections either of free horseradish peroxidase or of horseradish peroxidase conjugated with wheat germ agglutinin were given to albino rats in order to study the afferent connections of structures of the subthalamic region. The results suggest that the zona incerta receives its main input from several territories of the cerebral cortex, the mesencephalic reticular formation, deep cerebellar nuclei, regions of the sensory trigeminal nuclear complex and the dorsal column nuclei. Substantial input to the zona incerta appears to come from the superior colliculus, the anterior pretectal nucleus and the periaqueductal gray substance, whereas many other structures, among which hypothalamic nuclei, the locus coeruleus, the raphe complex, the parabrachial area and medial districts of the pontomedullary reticular formation, seem to represent relatively modest but consistent additional input sources. The afferentation of neurons in Forel's fields H1 and H2 appears to conform to the general pattern outlined above. As pointed out in the Discussion, the present results provide hodological support for the classic concept according to which the zona incerta can be regarded as a rostral extent of the midbrain reticular core. Some of the possible physiological correlates of the fiber connections of the zona incerta in the context of the sleep-waking cycle, ingestive behaviors, somatic motor mechanisms, visual functions and nociceptive behavior are briefly discussed.
Assuntos
Diencéfalo/anatomia & histologia , Vias Aferentes/anatomia & histologia , Vias Aferentes/fisiologia , Animais , Cerebelo/anatomia & histologia , Diencéfalo/fisiologia , Feminino , Bulbo/anatomia & histologia , Mesencéfalo/anatomia & histologia , Ponte/anatomia & histologia , Ratos , Formação Reticular/anatomia & histologia , Telencéfalo/anatomia & histologiaRESUMO
The afferent connections of the nuclei reticularis pontis oralis and caudalis were studied experimentally in the rat by the aid of either free horseradish peroxidase or horseradish peroxidase conjugated with wheat germ agglutinin used as retrograde tracers. The results suggest that the nucleus reticularis pontis oralis receives its main input from the zona incerta and field H1 of Forel, the superior colliculus, the central gray substance, and the mesencephalic and magnocellular pontomedullary districts of the reticular formation. Many other structures seem to represent modest additional sources of projections to the nucleus reticularis pontis oralis; these structures include numerous cortical territories, the nucleus basalis, the central amygdaloid nucleus, hypothalamic districts, the anterior pretectal nucleus, the substantia nigra, the cuneiform, the accessory oculomotor and the deep cerebellar nuclei, trigeminal, parabrachial and vestibular sensory cell groups, the nuclei raphe dorsalis and magnus, the locus coeruleus, the dorsolateral tegmental nucleus, and the spinal cord. While the afferentation of the rostral portion of the nucleus reticularis pontis caudalis appears to conform to the general pattern outlined above, some deviations from that pattern emerge when the innervation of the caudal district of the nucleus reticularis pontis caudalis is considered; the most striking of these differences is the fact that both spinal and cerebellar inputs seem to distribute much more heavily to the referred caudal district than to the remaining magnocellular pontine reticular formation. The present results may contribute to the elucidation of the anatomical substrate of the functionally demonstrated involvement of the nuclei reticularis pontis oralis and caudalis in several domains that include the regulation of the sleep-waking cycle and cortical arousal, somatic motor mechanisms and nociceptive behavior.
Assuntos
Ponte/anatomia & histologia , Formação Reticular/anatomia & histologia , Animais , Encéfalo/anatomia & histologia , Feminino , Peroxidase do Rábano Silvestre , Vias Neurais/anatomia & histologia , Ratos , Conjugado Aglutinina do Germe de Trigo-Peroxidase do Rábano Silvestre , Aglutininas do Germe de TrigoRESUMO
The afferent connections of the mesencephalic reticular formation were studied experimentally in the rat by the aid of the retrograde horseradish peroxidase tracer technique. The results suggest that the rostral portion of the mesencephalic reticular formation receives its main input from the cerebral cortex, the zona incerta and the fields of Forel, the central gray substance, the nuclei reticularis pontis oralis and caudalis, and the deep cerebellar nuclei. Substantial input to the same territory of the mesencephalic reticular formation appears to come from the superior colliculus, the substantia nigra, the parabrachial area, the spinal trigeminal nucleus, and the nucleus reticularis gigantocellularis, whereas several other brain structures, among which the locus coeruleus and the raphe complex, seem to represent modest but consistent additional input sources. The afferentation of more caudal portions of the mesencephalic reticular formation appears to conform to the general pattern outlined above with only three exceptions; the cerebral cortex, the deep cerebellar nuclei and the spinal trigeminal nucleus seem to be relatively modest sources of projections to these levels. Considering that the mesencephalic reticular formation is a critical structure in the "ascending activating systems" the present results, confirming and extending those of many other investigators, characterize a set of pathways that seem to be an important part of the anatomical substrate of the sleep-walking cycle.
Assuntos
Mesencéfalo/anatomia & histologia , Formação Reticular/anatomia & histologia , Vias Aferentes/anatomia & histologia , Tonsila do Cerebelo/anatomia & histologia , Animais , Cerebelo/anatomia & histologia , Córtex Cerebral/anatomia & histologia , Dominância Cerebral/fisiologia , Feminino , Globo Pálido/anatomia & histologia , Peroxidase do Rábano Silvestre , Bulbo/anatomia & histologia , Neurônios/ultraestrutura , Ponte/anatomia & histologia , Área Pré-Óptica/anatomia & histologia , Ratos , Núcleos Talâmicos/anatomia & histologiaRESUMO
An event recorder for use in ethological studies which is of simple and inexpensive construction is described. The apparatus consists essentially of a constant speed paper transport device and an ethogram sheet holder. Up to 20 different behavioral items may be recorded continuously in an accurate time sequence. The items are coded as hand-drawn pencil lines of different length perpendicular to the direction of the paper movement. Since the ethogram code sheets are exchangeable, an unlimited number of different ethograms may be used.
Assuntos
Comportamento Animal , Etologia/instrumentação , Registros , AnimaisRESUMO
Electrocorticographic activity was automatically recorded in albino rats for 72 consecutive h and analyzed by procedures suitable to detect 24-h rhythms. Beta (alert wakefulness), theta (somnolence), delta (slow wave sleep) and sigma 1 (superficial synchronized sleep) activities showed a robust circadian rhythmic distribution. The acrophases (maxima of the adjusted cosine curve) occurred at 23:39, 07:59, 08:37 and 13:25 h, respectively. EMG atonia and extreme hypotonia (less than 10% of mean EMG level) episodes showed a 24-h rhythm peaking at 14:18 h. The temporal sequence within the circadian rest period, i.e., somnolence, slow wave sleep, superficial synchronized sleep and paradoxical sleep, is very similar to that known to occur during the nocturnal sleep of humans.
Assuntos
Córtex Cerebral/fisiologia , Ritmo Circadiano , Eletroencefalografia , Sono/fisiologia , Animais , Nível de Alerta/fisiologia , Humanos , Masculino , Monitorização Fisiológica , Ratos , Fases do Sono , Especificidade da Espécie , Lobo Temporal/fisiologiaRESUMO
It is proposed that the corpus callosum has a major role in the processes of memory; first, by providing access by each hemisphere to memory traces stored in the other, and second, by controlling the formation of memory traces in such a way that they are laid down in only one hemisphere instead of in both. This dual mechanism would have the effect of doubling the mnemonic storage capacity of the brain. Evidence in favor of this hypothesis in briefly reviewed. It is also supported by experiments using electrical excitation of the striate cortex as a conditional stimulus in macaques, and by experiments on maze behavior in the m e species. Experiments with the anterior commissure, on the other hand, show that it, in contrast to the splenium of the corpus callosum, can actually transfer an engram from one hemisphere to the other. Finally, it is shown that the splenium provides an effective path of communication between the central visual system in one hemisphere and the amygdala in the other.
Assuntos
Tonsila do Cerebelo/fisiologia , Corpo Caloso/fisiologia , Memória , Córtex Visual/fisiologia , Animais , Condicionamento Psicológico , Estimulação Elétrica , Lobo Frontal/fisiologia , Lateralidade Funcional , Generalização do Estímulo , Haplorrinos , Aprendizagem , Macaca , Motivação , Vias Neurais/fisiologia , Quiasma Óptico/fisiologia , ReflexoRESUMO
This work describes the results of two experimental procedures related to the short latency median nerve somatosensory evoked potentials. In the first one, it was possible to show that there is a different rate of increase in the amplitude of the different components related to different intensities of stimulation. In the second one, the simultaneous stimulation of the median and the ulnar nerves disclosed an occlusive interaction which was larger for the N18 than for the other components. These results may be a support to the hypothesis that the N18 component generator is related to the mechanism of presynaptic inhibition within the cuneiform nucleus.
Assuntos
Eletroencefalografia , Potenciais Somatossensoriais Evocados/fisiologia , Nervo Mediano/fisiologia , Tempo de Reação/fisiologia , Adulto , Vias Aferentes/fisiologia , Mapeamento Encefálico , Estimulação Elétrica , Feminino , Humanos , Masculino , Bulbo/fisiologia , Pessoa de Meia-Idade , Inibição Neural/fisiologia , Nervo Ulnar/fisiologiaRESUMO
The electrocorticographic (ECoG) behavior of prepyriform cortex (PPC) was studied during the spontaneous sleep-wakefulness cycle (SWC) of freely-behaving rats in which recording electrodes had been chronically implanted in PPC, neocortex (NEO), entorhinal area, olfactory bulb (OB), hippocampus, and dorsal neck muscles. The different phases of the SWC were discerned by taking into account the NEO, OB and neck musculature electrical activities, and criteria were set for the identification of various PPC ECoG patterns. The existence of rather regular associations between the PPC ECoG and the various SWC phases was demonstrated, but it was also seen that these associations defied simplistic generalizations. Thus, whereas the level of ECoG activity (in terms of synchronization vs. desynchronization) was, in the majority of instances, equivalent in PPC and NEO in some SWC phases (alert wakefulness, synchronized sleep and paradoxical sleep), the same was not true in the case of the relaxed wakefulness, drowsiness, intermediary and preparadoxical phases. In the same context, when the time relationships between the alternations of different SWC phases and PPC ECoG patterns were analyzed, it became clear that although only rarely the PPC transition occurred after the correspondent SWC one, the former could either precede, or be simultaneous with the latter, depending on the particular SWC transition being considered. The neural control mechanisms possibly responsible for the coupling of PPC and NEo EcoG activities are briefly discussed.
Assuntos
Sono/fisiologia , Córtex Somatossensorial/fisiologia , Vigília/fisiologia , Animais , Eletroencefalografia , Eletrofisiologia , Masculino , RatosAssuntos
Sistema Límbico/fisiologia , Córtex Visual/fisiologia , Vias Visuais/fisiologia , Vias Aferentes/fisiologia , Animais , Dominância Cerebral/fisiologia , Eletroencefalografia , Eletromiografia , Potenciais Evocados , Masculino , Bulbo Olfatório/fisiologia , Ratos , Privação Sensorial , Fases do Sono/fisiologiaAssuntos
Comportamento Animal , Córtex Cerebral/fisiologia , Fases do Sono , Sono , Animais , Nível de Alerta , Eletrodos Implantados , Eletroencefalografia , Eletromiografia , Movimentos Oculares , Hipocampo/fisiologia , Sistema Límbico/fisiologia , Músculos/fisiologia , Pescoço , Postura , Ratos , Ratos EndogâmicosRESUMO
The activities of malic enzyme (ME) and glucose-6-phosphate dehydrogenase (G-6-PDH), two NADPH-generating lipogenic enzymes, were measured in brown adipose tissue (BAT) of rats undergoing various neurohormonal manipulations. Methimazole-induced hypothyroidism doubled the activity of these two enzymes but, surprisingly, triiodothyronine (T3) given to hypothyroid rats caused a time- and dose-dependent stimulation of up to three- to fourfold. Unilateral BAT denervation modestly reduced the activity of these enzymes (approximately 30%) and failed to prevent the stimulation induced by hypothyroidism, whereas growth hormone (GH) successfully blocked this effect of hypothyroidism. Insulin stimulated both enzymes regardless of the thyroid status but failed to abolish the inhibitory effect of GH. In intact rats, cold exposure caused a time-dependent increase in the activity of both ME and G-6-PDH, which reached 5.2- and 3-fold, respectively, after 96 h. This cold-induced stimulation was not observed in hypothyroid rats, but it was restored by physiological doses of thyroxine (800 ng.100 g body wt-1.24 h-1). Replacement with T3 (300 ng.100 g body wt-1.24 h-1), in contrast, did not have this effect. In hypothyroid rats with hemidenervation of BAT, norepinephrine (NE) modestly increased ME and G-6-PDH activities in the denervated side, with little or no effect in the intact side. Receptor-saturating doses of T3 (50 micrograms.100 g body wt-1.day-1 over 48 h) stimulated two- and threefold both enzymes in both sides, reducing or obliterating the effect of denervation. The data suggest a complex neurohormonal regulation of the activity of ME and G-6-PDH in BAT.(ABSTRACT TRUNCATED AT 250 WORDS)
Assuntos
Tecido Adiposo Marrom/enzimologia , Glucosefosfato Desidrogenase/metabolismo , Malato Desidrogenase/metabolismo , Tiroxina/fisiologia , Tri-Iodotironina/fisiologia , Tecido Adiposo Marrom/inervação , Animais , Temperatura Baixa , Denervação , Relação Dose-Resposta a Droga , Hipotireoidismo/enzimologia , Lipídeos/biossíntese , Masculino , NADP/metabolismo , Ratos , Ratos Sprague-Dawley , SimpatectomiaRESUMO
OBJECTIVE: To study the interference of mechanical vibration of the palm of the hand on the median nerve short-latency SEP components. METHODS: Electrically-elicited short-latency median nerve SEP were obtained before and during mechanical vibration (120 Hz) of the palm in two groups of normal individuals (6 in group I and 9 in group II). The amplitude of the different components was compared between the two conditions through non-parametric statistical tests. RESULTS: A significant reduction in the amplitude of the N9, P13/14 and N20 components was detected, however no overall significant changes were detected for the N18 component. CONCLUSIONS: Vibration interference reduced all studied components except the N18, these findings are interpreted as supporting evidence for the proposed association between the N18 component and the inhibitory activities elicited in the dorsal column nuclei.
Assuntos
Potenciais Somatossensoriais Evocados , Nervo Mediano/fisiologia , Vibração , Adulto , Feminino , Mãos/inervação , Humanos , Masculino , Pessoa de Meia-Idade , Tempo de Reação/fisiologiaRESUMO
To investigate the role of type II 5'-deiodinase (5'D-II) in the expression of mitochondrial uncoupling protein (UCP) in brown adipose tissue (BAT), we injected intact male rats with reverse (r) 3,5,3'-triiodothyronine (T3; 100 micrograms. 100 g body wt-1. day-1), an inhibitor of 5'D-II, for 2-5 days. UCP decreased by approximately 20% in rats kept at 28 degreesC and failed to increase during cold exposure (4 degreesC). Next, thyroxine treatment (1-10 micrograms. 100 g body wt-1. day-1) increased nuclear T3 in rats kept at 28 or 4 degreesC. In these rats, nuclear T3 correlated positively with UCP. In addition, T3 (1-50 micrograms. 100 g body wt-1. day-1) given to intact rats (5-15 days; 28 degreesC) induced an approximately twofold increase in UCP. In these T3-treated animals, the interscapular BAT thermal response to norepinephrine infusion also correlated positively with T3 dose and UCP content. Treatment with propranolol or reserpine failed to block the T3 induction of UCP (approximately 1.8- and approximately 2.3-fold). The results emphasize the importance of local 5'D-II and reveal an independent role of T3 in the expression of UCP.
Assuntos
Tecido Adiposo Marrom/efeitos dos fármacos , Tecido Adiposo Marrom/metabolismo , Proteínas de Transporte/metabolismo , Proteínas de Membrana/metabolismo , Sistema Nervoso Simpático/fisiologia , Tri-Iodotironina/farmacologia , Tecido Adiposo Marrom/inervação , Tecido Adiposo Marrom/fisiologia , Antagonistas Adrenérgicos/farmacologia , Animais , Regulação da Temperatura Corporal/efeitos dos fármacos , Regulação da Temperatura Corporal/fisiologia , Núcleo Celular/metabolismo , Temperatura Baixa , Inibidores Enzimáticos/farmacologia , Iodeto Peroxidase/antagonistas & inibidores , Canais Iônicos , Isoenzimas/antagonistas & inibidores , Masculino , Proteínas Mitocondriais , Norepinefrina/farmacologia , Ratos , Ratos Wistar , Receptores dos Hormônios Tireóideos/metabolismo , Tiroxina/farmacologia , Tri-Iodotironina/metabolismo , Tri-Iodotironina Reversa/farmacologia , Proteína Desacopladora 1RESUMO
Macaques were trained to respond to electrical excitation applied through electrodes permanently implanted within or upon striate cortex. Threshold current for the animal to detect this stimulation was highly consistent from day to day and, in the absence of tissue encapsulation of the electrodes or deliberately inflicted damage, remained stable indefinitely, 38 months in the longest case so far. Stimulating continuously for 1-8h, however, produces an elevation of threshold, which may be permanent or temporary, depending upon a variety of conditions. A major cause of such injury is the hydrolysis commonly occurring consequent to passage of low-level currents between solutions and metal electrodes. Even when the hydrolytic reaction is eliminated by restricting the level of electrode polarization or by using capacitative stimulation with tantalum pentoxide electrodes, a rise in threshold often still occurs with protracted stimulation. With proper control in some instances, however, effective stimulation at 2-10 times the threshold level could be maintained indefinitely without apparent injury, e.g. in a blind monkey having a threshold of 290 muA that could respond immediately to an 80-muA diminution in 580-muA, 0.2-msec stimulus pulses which had been applied steadily for 1 h at 50 Hz.
Assuntos
Estimulação Elétrica/efeitos adversos , Córtex Visual/fisiologia , Animais , Comportamento Animal , Estimulação Elétrica/instrumentação , Eletrodos Implantados , Reação a Corpo Estranho , Haplorrinos , Macaca , Próteses e Implantes , Fatores de Tempo , Percepção VisualRESUMO
UNLABELLED: To study the thermal response of interscapular brown fat (IBF) to norepinephrine (NE), urethan-anesthetized rats (1.2 g/kg ip) maintained at 28-30 degrees C received a constant venous infusion of NE (0-2 x 10(4) pmol/min) over a period of 60 min. IBF temperatures (T(IBF)) were recorded with a small thermistor fixed under the IBF pad. Data were plotted against time and expressed as maximal variation (Deltat degrees C). Saline-injected rats showed a decrease in T(IBF) of approximately 0.6 degrees C. NE infusion increased T(IBF) by a maximum of approximately 3.0 degrees C at a dose of 10(4) pmol x min(-1) x 100 g body wt(-1). Surgically thyroidectomized (Tx) rats kept on 0.05% methimazole showed a flat response to NE. Treatment with thyroxine (T(4), 0.8 microg x 100 g(-1) x day(-1)) for 2-15 days normalized mitochondrial UCP1 (Western blotting) and IBF thermal response to NE, whereas iopanoic acid (5 mg x 100 g body wt(-1) x day(-1)) blocked the effects of T(4). Treatment with 3,5, 3'-triiodothyronine (T(3), 0.6 microg x 100 g body wt(-1) x day(-1)) for up to 15 days did not normalize UCP1 levels. However, these animals showed a normal IBF thermal response to NE. Cold exposure for 5 days or feeding a cafeteria diet for 20 days increased UCP1 levels by approximately 3.5-fold. Nevertheless, the IBF thermal response was only greater than that of controls when maximal doses of NE (2 x 10(4) pmol/min and higher) were used. CONCLUSIONS: 1) hypothyroidism is associated with a blunted IBF thermal response to NE; 2) two- to fourfold changes in mitochondrial UCP1 concentration are not necessarily translated into heat production during NE infusion.
Assuntos
Tecido Adiposo Marrom/metabolismo , Regulação da Temperatura Corporal/fisiologia , Proteínas de Transporte/metabolismo , Proteínas de Membrana/metabolismo , Norepinefrina/metabolismo , Tecido Adiposo Marrom/efeitos dos fármacos , Animais , Regulação da Temperatura Corporal/efeitos dos fármacos , Temperatura Baixa , Dieta , Relação Dose-Resposta a Droga , Infusões Intravenosas , Canais Iônicos , Ácido Iopanoico/farmacologia , Masculino , Metimazol/farmacologia , Mitocôndrias/metabolismo , Proteínas Mitocondriais , Norepinefrina/administração & dosagem , Ratos , Ratos Wistar , Tireoidectomia , Tiroxina/antagonistas & inibidores , Tiroxina/sangue , Tiroxina/farmacologia , Tri-Iodotironina/sangue , Proteína Desacopladora 1RESUMO
Electrocorticographic activity was automatically recorded in albino rats for 72 consecutive h and analyzed by procedures suitable to detect 24-h rhythms. Beta (alert wakefulness), theta (somnolence), delta (slow wave sleep) and sigma 1 (superficial synchronized sleep) activities showed a robust circadian rhythmic distribution. The acrophases (maxima of the adjusted cosine curve) occurred at 23:39, 07:59, 08:37 and 13:25 h, respectively. EMG atonia and extreme hypotonia (less than 10% of mean EMG level) episodes showed a 24-h rhythm peaking at 14:18 h. The temporal sequence within the circadian rest period, i.e., somnolence, slow, wave sleep, superficial synchronized sleep and paradoxical sleep, is very similar to that known to occur during the nocturnal sleep of humans