RESUMO
This paper aimed to investigate the effects of Jinwu Jiangu recipe total extract on the IL-17/STAT3 signals in rheumatoid arthritis synovial fibroblasts(RASF). The primary RASFs were cultured by tissue piece method in vitroï¼ and divided into blank control groupï¼ Jinwu Jiangu recipe low dose groupï¼ Jinwu Jiangu recipe middle dose groupï¼ Jinwu Jiangu recipe high dose groupï¼ and tripterygium glycosides control group. They were then treated with corresponding serum free mediumï¼ different doses of Jinwu Jiangu recipe total extract(0.06ï¼ 0.6ï¼ 6.0 g·L⻹)ï¼ and tripterygium glycosides(0.03 g·L⻹) respectively for 24 hours. The gene expression levels of RORαï¼ RORγtï¼ and STAT3 mRNA were detected by polymerase chain reaction(PCR)ï¼ and the protein activity of IL-17R and pSTAT3 were measured by Western blot assay. The results showed that as compared with blank control groupï¼ the expression levels of RORαï¼ RORγtï¼ IL-17R and STAT3 mRNA in RASF were significantly declined(P<0.01). As compared with tripterygium glycosides control groupï¼ Jinwu Jiangu recipe total extract middle dose group and high dose group can down-regulate the expression levels of RORαï¼ RORγtï¼ IL-17R and STAT3 mRNA(P<0.05)ï¼ and the effect was more obvious in high dose group(P<0.01). As compared with blank control groupï¼ the protein expression levels of IL-17R and pSTAT3 in each treatment group were obviously decreased(P<0.01). As compared with tripterygium glycosides control groupï¼ Jinwu Jiangu recipe high dose group had more obvious effect in down-regulating the protein expression of pSTAT3(P<0.01). Thereforeï¼ Miao medicine Jinwu Jiangu recipe total extract can down-regulate the expressions of RORαï¼ RORγtï¼ and STAT3 mRNAï¼ and inhibit the protein activity of IL-17R and pSTAT3 in RASF.