[Use of immunospecific components labelled with beta-lactamase and horseradish peroxidase for immunoenzyme analysis]. / Ispol'zovanie immunospetsificheskikh komponentov, mechennykh beta-laktamazoi i peroksidazoi khrena dlia immunofermentnogo analiza.

A system for enzyme immunoassay with the use of beta-lactamase as an enzyme marker is described. Similar sensitivity of the enzyme immunoassays with the use of beta-lactamase and horse-raddish peroxidase as enzyme markers was shown. The data on decrease of the antigen binding capacity of the antibodies in the conjugates with beta-lactamase are presented. It is suggested that such a decrease may be associated with glutaric dialdehyde conjugation. The use of the schemes for enzyme immunoassay with simultaneous application of two independently recorded enzymes i. e. horse-raddish peroxidase and beta-lactamase provided confirmation of univalency of the conjugate of the melted capsular antigen of the plague causative agent with beta-lactamase. Identity of the antigenic determinants of the melted antigen and capsular antigen of the plague microbe was demonstrated.

[Quantitative dansyl micromethod of amino acid analysis. Study of peptide maps of ribonuclease tryptic hydrolysate]. / Kolichestvennyi dansil'nyi mikrometod analiza aminokislot. issledovanie peptidnykh kart tripticheskogo gidrolizata ribonukleazy.

Some variants of the quantitative dansyl method in combination with thin-layer chromatography on polyamide plates have been compared, and principles of its optimization have been demonstrated. Sensitivity of the method is about 10(-11) mole, the error does not exceed 10%. Data are given on the analysis by the dansyl method of peptides of the tryptic hydrolysate of bovine pancreatic ribonuclease after their separation by peptide mapping procedure.