RESUMO
In the field of nanomedicine, biogenic metal nanoparticles are commonly synthesized using edible plant products as bio-reducing or stabilizing agents. In this study, discarded shell of velvet tamarind fruit is explored as a potent reducing agent for biogenic synthesis of silver nanoparticles (VeV-AgNPs). Silver nanoparticles were formed in minutes under sunlight exposure, which was considerably fast compared to under ambient conditions. The optical, structural and morphological studies revealed that the nanoparticle colloidal solution consisted of particles with quasi-spherical and rodlike morphologies. To investigate antimicrobial properties, eight microorganisms were exposed to the VeV-AgNPs. The results indicated that VeV-AgNPs had enhanced antimicrobial activity, with a recorded minimum inhibitory concentration (MIC) of 3.9 µg/mL against E. coli. Further studies were conducted to examine the biofilm inhibition properties and synergistic effect of the VeV-AgNPs. The findings showed a biofilm inhibition potential of around 98% against E. coli, and the particles were also found to increase the efficacy of standard antimicrobial agents. The combinatory effect with standard antifungal and antibacterial agents ranged from synergistic to antagonistic effects against the tested microorganisms. These results suggest that silver nanoparticles produced from discarded shells of velvet tamarind are potent and could be used as a potential drug candidate to combat antimicrobial resistance.
Assuntos
Anti-Infecciosos , Nanopartículas Metálicas , Tamarindus , Prata/farmacologia , Prata/química , Nanopartículas Metálicas/química , Escherichia coli , Frutas , Anti-Infecciosos/farmacologia , Antibacterianos/química , Biofilmes , Testes de Sensibilidade Microbiana , Extratos Vegetais/farmacologia , Extratos Vegetais/químicaRESUMO
Surfaces serve as the communication link between the adsorbate and the substrate. Hence, a thorough understanding of the surface chemistries directly interfacing with biological molecules and other adsorbates would provide insight into the fabrication approach as well as the adsorption characteristics of biomolecules adsorbed on the surface. This paper presents a surface-enhanced Raman spectroscopy (SERS) method for high-sensitivity detection and reading of protein microarrays based on gold nanoparticle labels. The reagent employed was 30 nm gold nanoparticles modified with a bifunctional Raman reporter molecule, 5,5'-dithiobis(succinimidyl-2-nitrobenzoate) (DSNB), to integrate anti-bovine IgG for an antigen response in the immunoassay and generate an intense SERS signal. The signal from the DSNB reporter molecule, particularly the strong symmetric nitro stretch was used for the detection of antigen-antibody interactions. Issues related to the sensitivity and selectivity of the assay were also addressed. This work provides useful insights into SERS-based immunoassays and serves as the basis for an eventful adventure into interfacial biomolecular interactions.
Assuntos
Nanopartículas Metálicas , Análise Espectral Raman , Análise Espectral Raman/métodos , Ouro/química , Nanopartículas Metálicas/químicaRESUMO
This study characterizes and evaluates the constituents of produced water at production wells and dumping sites. The study examined the impact of offshore petroleum mining activities on aquatic systems for regulatory compliance and the selection of management and disposal options. The physicochemical analyses of produced water from the three study locations were within the permissible range for pH, temperature, and conductivity. Of the four heavy metals detected, mercury had the lowest concentration at 0.002 mg/L, while arsenic the metalloid, and iron had the highest concentrations at 0.038 mg/L and 36.1 mg/L, respectively. The total alkalinity values for the produced water in this study are about six-fold compared to the other three locations (Cape Three Point, Dixcove, University of Cape Coast). Compared to the other locations, produced water had higher toxicity to Daphnia, with an EC50 value of 80.3 %. The levels of polycyclic aromatic hydrocarbons (PAHs), volatile hydrocarbons, and polychlorinated biphenyls (PCBs) analyzed in this study were all insignificant in terms of toxicity. The total hydrocarbon concentrations indicated a high level of environmental impact. However, considering the possible breakdown of total hydrocarbons over time, and the marine ecosystem's high pH and salinity conditions, further recordings and observations should be conducted to ascertain the overall cumulative effects of oil drilling activities at the Jubilee oil fields along the shores of Ghana.
Assuntos
Monitoramento Ambiental , Poluentes Químicos da Água , Ecossistema , Hidrocarbonetos/análise , Indústria de Petróleo e Gás , Hidrocarbonetos Policíclicos Aromáticos/análise , Água/análise , Poluentes Químicos da Água/análiseRESUMO
Glancing angle deposition was used to produce approximately 150-nm-thick silver nanoparticle films, which were evaluated as localized surface plasmon resonance (LSPR) biosensors. The films have a strong extinction peak around 368 nm in air due to LSPR. As the refractive index of the surrounding environment is increased, the extinction peak red-shifts with a linear dependence. The films were functionalized with 11-amino-1-undecanethiol and rabbit immunoglobulin G (rIgG) to allow for the detection of anti-rIgG binding. Binding of biomolecules to the nanoparticle surface increases the local refractive index and results in a red-shifting of the extinction peak. The wavelength shift at varying concentrations of anti-rIgG was measured and fit to the Langmuir isotherm. This yielded approximate values for the saturation response, Delta lambda max = 29.4 +/- 0.7 nm, and the surface confined binding constant, Ka = (2.7 +/- 0.3) x 10(6) M(-1). The response to nonspecific binding was also investigated.