Interactions Between Heavy Metal Exposure and Blood Biochemistry in an Urban Population of the Black Swan (Cygnus atratus) in Australia.

There is growing recognition of the threat posed to wildlife by pollutants. Waterbirds are robust bioindicators of ecosystem health, and metal toxicity is a threat to these species in waterways worldwide. Urban waterbirds are likely to be at the highest risk of heavy metal exposure, but this issue has not been widely explored in Australia. Our aim was to estimate contemporary heavy metal exposure in a sedentary urban waterbird population: black swans (Cygnus atratus) inhabiting an inner-city wetland in one of Australia's largest cities, Melbourne. To investigate the physiological implications of legacy heavy metal exposure in these birds, we quantified blood biochemistry profiles and examined their relationships with metal concentrations in feathers. We caught 15 swans in 2021 and took feather samples to measure the concentration of eight heavy metals (chromium (Cr), manganese (Mn), iron (Fe), nickel (Ni), copper (Cu), zinc (Zn), lead (Pb), and mercury (Hg)), and blood samples to measure the concentration of 13 plasma analytes. Multivariate regression analysis revealed few associations between heavy metals and biochemistry markers, and no differences between sexes or age classes. This study presents a baseline dataset of these contaminants and blood biochemical profiles of swans at this wetland that can be used for future monitoring and is an important step toward a better understanding of the threat posed by heavy metals to Australian urban waterbirds.

The Toxicity of Nanoparticles to Organisms in Freshwater.

Nanotechnology is a rapidly growing industry yielding many benefits to society. However, aquatic environments are at risk as increasing amounts of nanoparticles (NPs) are contaminating waterbodies causing adverse effects on aquatic organisms. In this review, the impacts of environmental exposure to NPs, the influence of the physicochemical characteristics of NPs and the surrounding environment on toxicity and mechanisms of toxicity together with NP bioaccumulation and trophic transfer are assessed with a focus on their impacts on bacteria, algae and daphnids. We identify several gaps which need urgent attention in order to make sound decisions to protect the environment. These include uncertainty in both estimated and measured environmental concentrations of NPs for reliable risk assessment and for regulating the NP industry. In addition toxicity tests and risk assessment methodologies specific to NPs are still at the research and development stage. Also conflicting and inconsistent results on physicochemical characteristics and the fate and transport of NPs in the environment suggest the need for further research. Finally, improved understanding of the mechanisms of NP toxicity is crucial in risk assessment of NPs, since conventional toxicity tests may not reflect the risks associated with NPs. Behavioural effects may be more sensitive and would be efficient in certain situations compared with conventional toxicity tests due to low NP concentrations in field conditions. However, the development of such tests is still lacking, and further research is recommended.

The PAH body burdens and biomarkers of wild mussels in Port Phillip Bay, Australia and their food safety implications.

Wild Mediterranean mussels (Mytilus galloprovincialis) were collected from 10 coastal sites of Port Phillip Bay, Australia, and 16 types of Polycyclic aromatic hydrocarbons (PAHs) in their bodies were measured. Also, mussels from 4 of the 10 sites were sampled and the activities of Metallothioneins (MTs), superoxide dismutase (SOD), glutathione S-transferase (GST), and Ethoxyresorufin -o- deethylase (EROD) in the gills were tested. The results demonstrated spatial variation of different PAHs in mussels, which differed from trace metal accumulations to mussels reported in previous studies. Besides, the results of SOD, GST, and EROD showed highly similar features among mussels from all the sites while the results of MTs appeared to be different. The estimation of possible excessive cancer risks caused by wild mussel consumption using the results in this study demonstrated that the PAHs in wild mussels in Port Phillip Bay is a moderate concern for consumers.

Population- and sex-specific sensitivity of the marine amphipod Allorchestes compressa to metal exposure.

The sensitivity to contaminants of natural populations varies greatly depending on their historical exposure and on the sex of the individual. These factors result in great uncertainty in ecotoxicological risk assessments and challenge the protection of marine biodiversity. This study investigated the role of background pollution in the environment in shaping the sensitivity of males and females of the common marine amphipod Allorchestes compressa to the common trace marine pollutant, copper (Cu). Female and male amphipods were collected from two sites: Geelong (the polluted site) and Clifton Springs (the clean site). Amphipods were exposed to Cu treatments of 0, 50, 100, and 250 µg/L for 10 days, followed by a 10-day recovery period. Cu-exposed males from Geelong showed a reduction in feeding rate at a higher Cu concentration than males from Clifton Springs, suggesting that they have a higher tolerance to Cu than males from Clifton Springs. This can be explained by their higher base level of metallothioneins (MTs) and glutathione-S-transferase (GST), the key physiological responses for detoxification and defence against damages from Cu toxicity. Males showed a higher tolerance to Cu than females. This pattern was similar in both populations, which may be associated with a higher level of GST. During the recovery period, only males from Geelong fully recovered to the control level. Our results emphasize the importance of considering population- and sex-specific sensitivity of invertebrates to contaminants in ecotoxicological risk assessments.

Effects of perfluorooctanoic acid (PFOA) on the thyroid status, vitellogenin, and oxidant-antioxidant balance in the Murray River rainbowfish.

Perfluorooctanoic acid's (PFOA) widespread use, presence and persistence in the aquatic environment has led to an increasing number of studies focusing on its toxicological effects. In Australia, PFOA has been detected in the aquatic environment, however its effects on Australian native fauna are unknown. In this study, male Australian native fish Murray River rainbowfish (Melanotaenia fluviatilis) were exposed to four different concentrations of PFOA (0.01, 0.1, 1 and 10 mg L-1). Variations in thyroid hormones (Triiodothyronine (T3)/Thyroxine (T4)) and the presence of vitellogenin were determined in plasma. Oxidative stress responses were evaluated in gills and liver. Exposure of male fish to PFOA resulted in altered T3/T4 ratios and the presence of vitellogenin in the plasma. Activities of catalase (CAT) and glutathione- S-transferase (GST) were significantly increased in the gills and significantly reduced in the liver. Lipid peroxidation was observed in both tissues showing that vital organs could not neutralize the peroxides generated by oxidative stress resulting from exposure to PFOA. In natural populations exposed to PFOA, such hormonal disturbances can have negative effects, notably through altered capacity to respond to changes in environmental conditions.

The toxicity of non-aged and aged coated silver nanoparticles to the freshwater shrimp Paratya australiensis.

Nanoparticles (NPs) transform in the environment which result in alterations to their physicochemical properties. However, the effects of aging on the toxicity of NPs to aquatic organisms remain to be determined. Further the reports that have been published present contradictory results. The aim of this study was to examine the stability of differently coated silver nanoparticles (AgNPs) in media and the influence of aging of these NP on potential toxicity to freshwater shrimp Paratya australiensis. Coating-dependent changes in the stability of AgNP were observed with aging. Curcumin (C) coated AgNPs were stable, while tyrosine (T) coated AgNPs and epigallocatechin gallate (E) coated AgNPs aggregated in the P. australiensis medium. Increased lipid peroxidation and catalase activity was noted in P. australiensis exposed to AgNPs, suggesting oxidative stress was associated with NP exposure. The enhanced oxidative stress initiated by aged C-AgNPs suggests that aging of these NPs produced different toxicological responses. In summary, data suggest that coating-dependent alterations in NPs, together with aging affect both persistence and subsequent toxicity of NPs to freshwater organisms. Thus, the coating-dependent fate and toxicity of AgNPs together with the effect of their aging need to be considered in assessing the environmental risk of AgNPs to aquatic organisms.

Acute and chronic toxicity of magnesium to the early life stages of two tropical freshwater mussel species.

Magnesium (Mg) is a common contaminant in mine water discharges. Although Mg is an essential element in biological processes, increased concentrations from anthropogenic sources can stress aquatic ecosystems. Additionally, studies evaluating the effects of Mg on north Australian freshwater species have indicated that in very soft waters there is a high risk to some species. Freshwater mussels are an ecologically and culturally important taxon in many freshwater environments, but knowledge of their sensitivity to Mg is limited. In the present study, the acute and chronic sensitivity of two freshwater mussel species, Velesunio angasi and an undescribed Velesunio species, to Mg was assessed (using MgSO4) on their early life stages, larval glochidia and post-parasitic juveniles. Acute 24-h exposures with glochidia generated a mean median lethal (LC50) toxicity estimate of 284mg/L for the five tests with V. angasi, and a mean LC50 of 300mg/L for the three tests with Velesunio sp. Mean chronic 14-d toxicity estimates resulting in 50% (EC50) and 10% (EC10) growth rate reductions for juveniles were 241 and 88mg/L respectively for the three tests with V. angasi juveniles, and 232 and 87mg/L respectively for the three tests with Velesunio sp. juveniles. The results represent the first acute and chronic Mg toxicity data for tropical freshwater mussels, and indicated that V. angasi and Velesunio sp. exhibited similar sensitivity and were moderately sensitive to Mg when compared to other tropical species. These results are a valuable contribution to the small existing dataset for Mg toxicity to tropical freshwater species, which can be used to inform water management in areas where Mg is a contaminant of concern, and ensure the protection of these taxa.

Assessing interactive mixture toxicity of carbamate and organophosphorus insecticides in the yabby (Cherax destructor).

Carbamate (CB) and organophosphorus (OP) pesticides are commonly detected in aquatic ecosystems and predominantly occur as mixtures of varying complexity. These pesticides inhibit the activity of total cholinesterase (ChE) and thus have the potential to interfere with behaviours that may be essential for the survival of aquatic species. Although the effects of individual ChE insecticides on aquatic species have been reported for decades, the neurotoxicity of mixtures is still poorly understood. This study examined the chronic toxicities of two OP insecticides (chlorpyrifos (CPF) and malathion (MAL)) and one carbamate insecticide (methomyl (METH)) in binary and ternary mixtures on the ChE activity of the yabby (C. destructor). Using the concentration addition approach to estimate mixture toxicity, the observed inhibition of ChE activity caused by all binary mixtures of CPF plus MAL, CPF plus METH and MAL plus METH was additive. In ternary mixtures, all combinations of CPF, MAL and METH were either additive or antagonistic depending on the relative ratios of these chemicals in the mixtures. The effect of mixtures of these three insecticides on C. destructor has not previously been assessed, and the data suggest that individual chemical risk assessments are likely to incorrectly estimate the effect of these insecticides on C. destructor in the aquatic environment where combinations of such chemicals occur.

Effects of environmental chemicals on fish thyroid function: Implications for fisheries and aquaculture in Australia.

Numerous environmental stressors exert acute or chronic effects on the fish thyroid cascade. Such effects could be mediated via thyroidal alterations, imbalance of plasma T4 and T3 levels or damage to the structure of the thyroidal tissues (thyroid hypertrophy, hyperplasia). The thyroidal system is intricately linked to other endocrine systems in vertebrates including the control of reproduction. Disruption of fish thyroid function by environmental stressors has the potential to result in deleterious effects including the inhibition of sperm production, reduction in egg production, gonad development, ovarian growth, swimming activity, fertilisation and increase in larval mortality. Thyroid hormones play a major role in the development and growth of fish, particularly during their early life stages, thus, thyroid disruption by environmental stressors could inhibit the growth of fish larvae and juveniles in wild fish and cultured species, limit fish seed production and result in a decline in wild fisheries. This review highlights the effects of several environmental toxicants including PBDE, PCBs, PCDD and PCDF, PAH/oil, phthalates, metals, pesticides, mixed pollutants/chemicals, cyanide; and other stressors including acid (low pH) and ammonia, on fish thyroid function. Environmental sources of chemical stressors and appropriate water quality guidelines to protect the freshwater and marine species for the relevant pollutants are also discussed including (when available) the Australian guidelines (2000) and Canadian water quality guidelines (where Australian guidelines are not available). To date there has been no published research on the effects of anthropogenic environmental pollutants on the thyroid system of any native Australian fish species. However, the detection of high risk chemicals (notably PBDEs, PCBs, PAHs, metals and pesticides) in Australian waterways and Australian fish and shellfish implies that thyroid disruption of Australian wild fish and aquacultured species could occur. It is therefore imperative that the effects of such pollutants on the thyroid system of Australian native fish be investigated.

Evaluating the non-lethal effects of organophosphorous and carbamate insecticides on the yabby (Cherax destructor) using cholinesterase (AChE, BChE), Glutathione S-Transferase and ATPase as biomarkers.

The toxicity of two organophosphorus insecticides, chlorpyrifos (CPF), malathion (MAL), and one carbamate insecticide, methomyl (METH), to the yabby (Cherax destructor) was assessed by measuring cholinesterase (AChE, BChE), Glutathione S-Transferase (GST) and Na+/K+ATPase activity after 96h of exposure. Yabbies exposed to all three insecticides at 2 and 5µgL-1 exhibited significant AChE, BChE, GST and Na+/K+ATPase inhibition. Based on these enzyme inhibition tests, the toxicity of the three insecticides to C. destructor was CPF > MAL > METH. After 14 days of recovery the yabbies enzymatic activities of AChE, BChE, GST and Na+/K+ATPase was measured. Recovery of The enzyme activity recovery was faster after the exposure to METH than for the yabbies exposed to CPF and MAL. Slow recovery of enzyme activity could affect the physical activities of organisms and produce indirect effects on populations if such crayfish are less able to elude predators or search for food.

Chemical Pollutants Sorbed to Ingested Microbeads from Personal Care Products Accumulate in Fish.

The prevalence of microplastics (<5 mm) in natural environments has become a widely recognized global problem. Microplastics have been shown to sorb chemical pollutants from their surrounding environment, thus raising concern as to their role in the movement of these pollutants through the food chain. This experiment investigated whether organic pollutants sorbed to microbeads (MBs) from personal care products were assimilated by fish following particle ingestion. Rainbow fish (Melanotaenia fluviatilis) were exposed to MBs with sorbed polybrominated diphenyl ethers (PBDEs; BDE-28, -47, -100, -99, -153, -154, -183, 200 ng g(-1); BDE-209, 2000 ng g(-1)) and sampled at 0, 21, 42, and 63 days along with two control treatments (food only and food + clean MBs). Exposed fish had significantly higher Σ8PBDE concentrations than both control treatments after just 21 days, and continued exposure resulted in increased accumulation of the pollutants over the experiment (ca. 115 pg g(-1) ww d(-1)). Lower brominated congeners showed the highest assimilation whereas higher brominated congeners did not appear to transfer, indicating they may be too strongly sorbed to the plastic or unable to be assimilated by the fish due to large molecular size or other factors. Seemingly against this trend, however, BDE-99 did not appear to bioaccumulate in the fish, which may be due to partitioning from the MBs or it being metabolized in vivo. This work provides evidence that MBs from personal care products are capable of transferring sorbed pollutants to fish that ingest them.

Automated Lab-on-a-Chip Technology for Fish Embryo Toxicity Tests Performed under Continuous Microperfusion (µFET).

The fish embryo toxicity (FET) biotest has gained popularity as one of the alternative approaches to acute fish toxicity tests in chemical hazard and risk assessment. Despite the importance and common acceptance of FET, it is still performed in multiwell plates and requires laborious and time-consuming manual manipulation of specimens and solutions. This work describes the design and validation of a microfluidic Lab-on-a-Chip technology for automation of the zebrafish embryo toxicity test common in aquatic ecotoxicology. The innovative device supports rapid loading and immobilization of large numbers of zebrafish embryos suspended in a continuous microfluidic perfusion as a means of toxicant delivery. Furthermore, we also present development of a customized mechatronic automation interface that includes a high-resolution USB microscope, LED cold light illumination, and miniaturized 3D printed pumping manifolds that were integrated to enable time-resolved in situ analysis of developing fish embryos. To investigate the applicability of the microfluidic FET (µFET) in toxicity testing, copper sulfate, phenol, ethanol, caffeine, nicotine, and dimethyl sulfoxide were tested as model chemical stressors. Results obtained on a chip-based system were compared with static protocols performed in microtiter plates. This work provides evidence that FET analysis performed under microperfusion opens a brand new alternative for inexpensive automation in aquatic ecotoxicology.

Assimilation of polybrominated diphenyl ethers from microplastics by the marine amphipod, Allorchestes compressa.

Microplastic particles (MPPs; <5 mm) are found in skin cleansing soaps and are released into the environment via the sewage system. MPPs in the environment can sorb persistent organic pollutants (POPs) that can potentially be assimilated by organisms mistaking MPPs for food. Amphipods (Allorchestes compressa) exposed to MPPs isolated from a commercial facial cleansing soap ingested ≤45 particles per animal and evacuated them within 36 h. Amphipods were exposed to polybrominated diphenyl ether (PBDEs) congeners (BDE-28, -47, -99, -100, -153, -154, and -183) in the presence or absence of MPPs. This study has demonstrated that PBDEs derived from MPPs can be assimilated into the tissue of a marine amphipod. MPPs reduced PBDE uptake compared to controls, but they caused greater proportional uptake of higher-brominated congeners such as BDE-154 and -153 compared to BDE-28 and -47. While MPPs in the environment may lower PBDE uptake compared to unabsorbed free chemicals, our study has demonstrated they can transfer PBDEs into a marine organism. Therefore, MPPs pose a risk of contaminating aquatic food chains with the potential for increasing public exposure through dietary sources. This study has demonstrated that MPPs can act as a vector for the assimilation of POPs into marine organisms.

A national survey of trace organic contaminants in Australian rivers.

Trace organic contaminant (TrOC) studies in Australia have, to date, focused on wastewater effluents, leaving a knowledge gap of their occurrence and risk in freshwater environments. This study measured 42 TrOCs including industrial compounds, pesticides, and pharmaceuticals and personal care products by liquid chromatography tandem mass spectrometry at 73 river sites across Australia quarterly for 1 yr. Trace organic contaminants were found in 92% of samples, with a median of three compounds detected per sample (maximum 18). The five most commonly detected TrOCs were the pharmaceuticals salicylic acid (82%, maximum = 1530 ng/L), paracetamol (also known as acetaminophen; 45%, maximum = 7150 ng/L), and carbamazepine (27%, maximum = 682 ng/L), caffeine (65%, maximum = 3770 ng/L), and the flame retardant (2-chloroethyl) phosphate (44%, maximum = 184 ng/L). Pesticides were detected in 28% of the samples. To determine the risk posed by the detected TrOCs to the aquatic environment, hazard quotients were calculated by dividing the maximum concentration detected for each compound by the predicted no-effect concentrations. Three of the 42 compounds monitored (the pharmaceuticals carbamazepine and sulfamethoxazole and the herbicide simazine) had a hazard quotient >1, suggesting that they may be causing adverse effects at the most polluted sites. A further 10 compounds had hazard quotients >0.1, indicating a potential risk; these included four pharmaceuticals, three personal care products, and three pesticides. Most compounds had hazard quotients significantly <0.1. The number of TrOCs measured in this study was limited and further investigations are required to fully assess the risk posed by complex mixtures of TrOCs on exposed biota.

Background concentrations and spatial distribution of PFAS in surface waters and sediments of the greater Melbourne area, Australia.

A survey of per- and polyfluoroalkyl substances (PFAS) was conducted in Melbourne, Australia to determine background concentrations in residential, industrial, municipal wastewater treatment plants, and rural land uses. Surface water and sediment samples collected from 65 sites with different catchment land uses were analysed for thirty-three PFAS. Twenty-two out of thirty-three targeted PFAS were detected, with at least one PFAS species was detected in 98% water samples and 8% sediment samples. One site was determined to have point-source pollution from an airport (surface water Σ33PFAS = 4261 ng/L) and was excluded from statistical analyses. The median Σ33PFAS concentration in surface water was 63.5 ng/L and the average was 78.6 ng/L (range < DL-526 ng/L). PFAS species with the highest median concentrations were PFBA (11.3 ng/L), PFHxA (9.2 ng/L), PFOA (8.3 ng/L), PFOS (8.0 ng/L), PFPeA (7.5 ng/L), PFHpA (3.2 ng/L), and PFHxS (2.9 ng/L). The average Σ4PFAS in sediments was 0.35 ng/g d.w. (range =

Environmentally relevant concentrations of chemically complex shale gas wastewater led to reduced fitness of water fleas (Daphnia carinata): Multiple lines of evidence approach.

Shale gas hydraulic fracturing generates flowback waters that pose a threat to aquatic organisms if released into the environment. In order to prevent adverse effects on aquatic ecosystems, multiple lines of evidence are needed to guide better decisions and management actions. This study employed a multi-disciplinary approach, combining direct toxicity assessment (DTA) on the water flea Daphnia carinata and LC-MS metabolomics analysis to determine the impact of a major ion salinity control (SC) and a cumulative flowback shale gas wastewater (SGW) from a well in the Beetaloo Sub-basin, Northern Territory, Australia. The exposures included a culture water control, simply further referred to as 'control', SC at 1% and 2% (v/v) and SGW at 0.125, 0.25, 0.5, 1% and 2% (v/v). The results showed that reproduction was significantly increased at SGW 0.5%, and significantly decreased when exposed to SC 2%. SGW 2% was found to be acutely toxic for the D. carinata (< 48-h). Second generation (F1) of D. carinata exposed to 0.125-1% SGW generally saw reduced activity in four oxidative biomarkers: glutathione S-transferase, lipid peroxidation, reactive oxygen species, and superoxide dismutase. At the metabolomics level, we observed significant changes in 103 metabolites in Daphnia exposed to both SGW and elevated salinity, in comparison to the control group. These changes indicate a range of metabolic disturbances induced by SGW and salinity, such as lipid metabolism, amino acid metabolism, nucleotide synthesis, energy production, and the biosynthesis of crucial molecules like hormones and pigments. These multiple lines of evidence approach not only highlights the complexities of SGW's impact on aquatic ecosystems but also underscores the importance of informed decision-making and management practices to safeguard the environment and its inhabitants.

Exposure to Persistent Organic Pollutants in Australian Waterbirds.

There is growing worldwide recognition of the threat posed by persistent organic pollutants (POPs) to wildlife populations. We aimed to measure exposure levels to POPs in a Southern Hemisphere aquatic waterbird species, the nomadic gray teal (Anas gracilis), which is found across Australia. We collected wings from 39 ducks harvested by recreational hunters at two sites (one coastal, one inland) in Victoria, southeastern Australia, in 2021. We examined three groups of POPs: nine congeners of polychlorinated biphenyls (PCBs), 13 organochlorine pesticides (OCPs), and 12 polycyclic aromatic hydrocarbons (PAHs). The PCBs, OCPs, and PAHs were detected at quantifiable levels in 13%, 72%, and 100% of birds, respectively. Of the congeners we tested for in PCBs, OCPs, and PAHs, 33%, 38%, and 100% were detected at quantifiable levels, respectively. The highest levels of exposure to POPs that we found were to the PAH benzo[b]fluoranthene, occurring at a concentration range of 1.78 to 161.05 ng/g wet weight. There were some trends detected relating to differences between geographical sites, with higher levels of several PAHs at the coastal versus inland site. There were several strong, positive associations among PAHs found. We discuss potential sources for the POPs detected, including industrial and agricultural sources, and the likely role of large-scale forest fires in PAH levels. Our results confirm that while Australian waterbirds are exposed to a variety of POPs, exposure levels are currently relatively low. Additional future investigations are required to further characterize POPs within Australian waterbird species. Environ Toxicol Chem 2024;43:736-747. © 2023 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.

Mixture Toxicity of Three Unconventional Gas Fracking Chemicals, Barium, O-Cresol, and Sodium Chloride, to the Freshwater Shrimp Paratya australiensis.

The 96-h acute toxicity of barium (Ba2+ ), o-cresol, and sodium chloride (NaCl) to Paratya australiensis was assessed in single, binary, and ternary combinations in addition to three biochemical assays: glutathione S-transferase, acetylcholinesterase, and sodium-potassium adenosine triphosphatase. The 96-h lethal concentrations that expressed 50% mortality (LC50) in the single-toxicant exposures were Ba2+ = 23.4 mg/L, o-cresol = 12.2 mg/L, and NaCl = 4198 mg/L. Mortality from o-cresol exposure occurred between 11 and 22 mg/L, whereas Ba2+ was more gradual across 10-105 mg/L, and most of the NaCl mortality occurred between 2050 and 4100 mg/L. Toxic units were used to assess the binary and ternary interactions of the toxicants. A more than additive effect was observed for most combinations in the binary chemical exposures, with the ternary combinations yielding highly synergistic interactions. Greater synergism was observed with the 96-h LC50 of o-cresol in combination with the three concentrations of NaCl (1025, 2050, and 3075 mg/L) compared with Ba2+ , with toxic units of 0.38, 0.48, and 0.10 (o-cresol) and 0.71, 0.67, and 0.50 (Ba2+ ). No notable enzyme activity trends were observed in the enzyme biomarker responses from both individual and mixture exposures. Although acute single-species toxicity tests tend to underestimate the effects of Ba2+ , o-cresol, and NaCl on populations, communities, and ecosystems in seminatural (e.g., mesocosms) and natural systems, there are currently no published acute toxicity data available for P. australiensis and the three toxicants used in the present study. The present study shows that chemicals with different toxicity mechanisms can potentially lead to more synergistic responses. Environ Toxicol Chem 2023;42:481-494. © 2022 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.

Environmental organic contaminant body burdens and GC-MS based untargeted metabolomics in mediterranean mussels from Port Phillip Bay, Australia☆.

Mussels were collected from four coastal sites around Port Phillip Bay, Australia in Mar and Apr 2021). Body burdens of Polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs), and organochlorine pesticides (OCPs) were measured and the possible sources of toxicants discussed. In addition, a gas chromatography-mass spectrometry (GC-MS) based untargeted metabolomics analysis was performed using the mantle tissues of mussels. Correlations between the results of contaminant body burdens and metabolic variations were investigated. The results demonstrated that high accumulations of low-molecular-weight PAHs were found in mussels. High body burdens of PCBs and OCPs were only found at mussels from the site close to the river mouth. Some of the metabolic pathways were correlated with the accumulation of PAHs. No correlations were found between PCB and OCP accumulations and metabolic abundances. According to the food and environmental standards of the European Union (EU), the PAH, PCB, and OCP accumulation in mussels in this study are a serious food safety concern.

Changes in metabolic profiles of amphipods Allorchestes compressa after acute exposures to copper, pyrene, and their mixtures.

Amphipods are ideal indicators for biomonitoring and ecotoxicological studies of environmental contaminants because they are extensively distributed in aquatic environments, are easy to collect and are important in nutrient cycling. Marine amphipods (Allorchestes compressa) were exposed to two concentrations of copper and pyrene, and their mixtures, for 24 and 48 h. Changes in polar metabolites were assessed using Gas Chromatography Mass Spectrometry (GC-MS)-based untargeted metabolomics. Generally, limited metabolite changes were observed for copper and pyrene single exposures (eight and two significant metabolites, respectively), while 28 metabolites had changed following exposures to mixtures. Furthermore, changes were mainly observed after 24 h but had seemingly returned to control levels after 48 h. Multiple types of metabolites were affected including amino acids, Tricarboxylic acid (TCA) cycle intermediates, sugars, fatty acids, and hormones. This study highlights the sensitivity of metabolomics in assessing the impacts of low concentrations of chemicals compared to traditional ecotoxicological endpoints.