RESUMO
In May 2016, two cases of tick-borne encephalitis (TBE) were confirmed by serology (positive IgM and IgG antibodies against TBE virus (TBEV) in serum), with a possible link to raw milk and cheese from a goat farm in a region in Baden-Württemberg, Germany not previously known as TBE-endemic. The outbreak investigation identified 32 consumers of goat dairy products (29 consumers, one farm employee, two owners) of whom none had IgM antibodies against TBEV 3-8 weeks after consumption. Of the 27 notified TBE cases in the State, none reported consumption of raw goat milk or cheese from the suspected farm. Five of 22 cheese samples from 18 different batches were RT-qPCR-positive for TBEV -genome, and two of the five samples were confirmed by virus isolation, indicating viability of TBEV in the cheese. Nine of the 45 goats had neutralising TBEV antibodies, two of them with a high titre indicating recent infection. One of 412 Ixodes ricinus was RT-qPCR-positive, and sequencing of the E gene from nucleic acid extracted from the tick confirmed TBEV. Phylogenetic analyses of tick and cheese isolates showed 100% amino acid homology in the E gene and a close relation to TBEV strains from Switzerland and Austria.
Assuntos
Queijo/virologia , Vírus da Encefalite Transmitidos por Carrapatos/isolamento & purificação , Encefalite Transmitida por Carrapatos/diagnóstico , Ixodes/virologia , Leite/virologia , Animais , Anticorpos Antivirais/sangue , Vírus da Encefalite Transmitidos por Carrapatos/genética , Encefalite Transmitida por Carrapatos/virologia , Cabras , Humanos , RNA Viral/sangue , RNA Viral/isolamento & purificação , Reação em Cadeia da Polimerase em Tempo Real , Análise de Sequência de DNARESUMO
BACKGROUND: The production of IgE molecules specific to the carbohydrate galactose-α-1,3-galactose (alpha-gal) is known to induce delayed anaphylaxis against mammalian meat. Tick bites constitute the primary sensitization source, as ticks transfer alpha-gal in their saliva to a host during a bite. The reported prevalence of alpha-gal-specific IgE (alpha-gal-sIgE) positivity varies between different populations from diverse geographic regions. OBJECTIVE: To investigate the prevalence of alpha-gal-sIgE positivity in a population of forest service employees who are highly exposed to ticks in comparison with a residential population and a historic sample. METHODS: A cross-sectional study evaluating 300 forest service employees and hunters from southwest Germany was performed. Alpha-gal-sIgE levels were assessed by ImmunoCAP assay. The prevalence of alpha-gal-sIgE-positive individuals was compared with a matched cohort composed of a residential population and blood samples from forest service employees collected 15 years ago. RESULTS: In the study population, the prevalence of alpha-gal-sIgE-positive (≥0.10 kUA /L) individuals was 35.0%, whereas the prevalence of individuals with alpha-gal-sIgE levels ≥0.35 kUA /L was 19.3%. Alpha-gal-sIgE positivity was associated with total IgE levels and recent tick bites. Mammalian meat-induced delayed anaphylaxis was found in 8.6% of the participants with alpha-gal-sIgE levels ≥0.35 kUA /L. For forest service employees and hunters, the odds ratio for alpha-gal-sIgE positivity was 2.48 compared to the residential population. The prevalence of alpha-gal-sIgE positivity in the current and historic cohort was comparable. CONCLUSION: Forest service employees and hunters compose a population with a high prevalence of alpha-gal-sIgE positivity and carry a considerable risk of red meat allergy.
Assuntos
Alérgenos/imunologia , Florestas , Galactose/imunologia , Hipersensibilidade Imediata/epidemiologia , Hipersensibilidade Imediata/imunologia , Exposição Ocupacional/efeitos adversos , Adulto , Idoso , Especificidade de Anticorpos/imunologia , Exposição Ambiental/efeitos adversos , Feminino , Humanos , Imunoglobulina E/sangue , Imunoglobulina E/imunologia , Masculino , Pessoa de Meia-Idade , Prevalência , Vigilância em Saúde Pública , Estações do Ano , Picadas de CarrapatosRESUMO
Our report concerns a 24-year-old man with a chronic exsudative skin lesion after a journey to Southeast Asia. The diagnosis of melioidosis was made by the identification of Burkholderia pseudomallei from the ichor. The diagnosis was confirmed by polymerase change reaction. The patient was treated with meropenem i. v. for about 10 days and with trimethoprim/sulfamethoxazole for the following 12 weeks. Melioidosis is an endemic disease in Southeast Asia and North Australia which in some cases can run a severe course and can have a high fatality rate. The relevance of melioidosis becomes more important against the background of the increasing global movement of travelers and migration.
Assuntos
Burkholderia pseudomallei/isolamento & purificação , Melioidose/diagnóstico , Úlcera Cutânea/microbiologia , Doença Relacionada a Viagens , Antibacterianos/uso terapêutico , Sudeste Asiático , Doença Crônica , Humanos , Masculino , Melioidose/tratamento farmacológico , Meropeném , Úlcera Cutânea/tratamento farmacológico , Tienamicinas/uso terapêutico , Adulto JovemRESUMO
Ticks and tick-borne diseases are of great significance for the health of humans and animals. However, the factors influencing their distribution and dynamics are inadequately known. In a project financed by the Baden-Württemberg Ministry of the Environment, Climate and Energy Industry, as part of the program BWPLUS, interdisciplinary specialists work together to determine the influence of weather, (micro)climate, habitat, land use, human activities, and the population dynamics of host animals on the distribution and abundance of ticks and the diseases that they transmit in Baden-Württemberg. The project comprises four modules: the large-scale distribution of ticks in Baden-Württemberg (module 1), detailed studies of host-tick-pathogen interaction in relation to the microclimate (module 2), and the spatial occurrence of important tick-borne pathogens (module 3). The fourth module involves the comprehensive analysis and synthesis of all data in order to determine the relative importance of the factors studied and to develop a risk model. Recently, intensive investigations into tick control have been undertaken using various entomopathogenic fungi and nematodes as well as a parasitoid wasp. Our aim was to determine whether these natural enemies could be used to effectively reduce the number of free-living ticks.
Assuntos
Ecossistema , Controle Biológico de Vetores/estatística & dados numéricos , Vigilância da População/métodos , Controle de Ácaros e Carrapatos/métodos , Doenças Transmitidas por Carrapatos/epidemiologia , Doenças Transmitidas por Carrapatos/prevenção & controle , Clima , Alemanha/epidemiologia , Programas Governamentais , Humanos , Controle Biológico de Vetores/métodos , Prevalência , Medição de Risco , Análise Espaço-Temporal , Controle de Ácaros e Carrapatos/estatística & dados numéricosAssuntos
Controle de Doenças Transmissíveis/estatística & dados numéricos , Doenças Transmissíveis/epidemiologia , Doenças Transmissíveis/parasitologia , Saúde Global , Controle de Insetos/estatística & dados numéricos , Vigilância da População/métodos , Animais , Controle de Doenças Transmissíveis/métodos , Alemanha/epidemiologia , Humanos , Controle de Insetos/métodos , Insetos Vetores , Prevalência , Medição de Risco , Vacinação/métodosRESUMO
Canine babesiosis in Europe is generally caused by Babesia canis canis and Babesia canis rossi. Here we describe the first two autochthonous cases of Babesia gibsoni (Asian genotype) infection in Germany. Two American pit bull terriers showed clinical and hematologic signs consistent with babesiosis. Polymerase chain reaction (PCR) analysis of the 18S rDNA of blood samples revealed 486 bp fragments. The sequences were 100% identical to each other and 100% identical to Babesia gibsoni (Asian genotype). These results represent the first genetic evidence of Babesia gibsoni (Asian genotype) parasites in dogs in Western Europe.
Assuntos
Babesia/isolamento & purificação , Babesiose/veterinária , DNA de Protozoário/análise , Doenças do Cão/epidemiologia , Animais , Babesiose/epidemiologia , Babesiose/parasitologia , DNA Ribossômico/análise , Doenças do Cão/parasitologia , Cães , Genótipo , Alemanha/epidemiologia , Reação em Cadeia da Polimerase/métodos , Reação em Cadeia da Polimerase/veterinária , Alinhamento de SequênciaRESUMO
Clinical standards to confirm babesiosis in dogs include the direct identification of the infectious agent in blood smears and serological assays for Babesia canis-specific antibodies. Here, we demonstrate in seven cases (with data on anamnesis, clinics, laboratory diagnostics, and therapeutic outcomes) that a new diagnostic procedure is required. This is the molecular-genetic identification of babesia by real time PCR allowing an unequivocal identification of the infectious agents. Indeed, all seven patients presenting severe clinical symptoms were PCR-positive, but only two of them had specific antibodies and showed babesia in their bloodstream. Six of the dogs appeared to have acquired babesiosis while travelling abroad, and one in the Swiss canton of Schaffhausen.
Assuntos
Babesia/isolamento & purificação , Babesiose/veterinária , DNA de Protozoário/análise , Doenças do Cão/diagnóstico , Reação em Cadeia da Polimerase/veterinária , Animais , Anticorpos Antiprotozoários/sangue , Antiprotozoários/uso terapêutico , Babesia/genética , Babesiose/diagnóstico , Babesiose/tratamento farmacológico , Diagnóstico Diferencial , Doenças do Cão/tratamento farmacológico , Cães , Feminino , Masculino , Reação em Cadeia da Polimerase/métodos , Sensibilidade e Especificidade , Resultado do TratamentoRESUMO
This study examined the occurrence of Coxiella burnetii (C. burnetii), the infectious agent of Q-fever, in sheep and sheep-ticks in Baden-Wuerttemberg, Germany, as a possible source of infection in Q-fever outbreaks. Using PCR, we examined a total of 1066 Dermacentor ticks from 23 herds and 49 samples of tick excrement from 18 herds for C. burnetii. We found the infectious agent in one non-engorged tick and in one sample of tick excrement from the same herd, in Efringen-Kirchen (district Loerrach). Sequencing the PCR-products confirmed the amplifications as specific for C. burnetii. Further serological tests of random samples of the four districts of Baden-Wuerttemberg showed a seroprevalence from 0 to 1.4% using complement fixation test (CFT), as well as a 0.9 to 10.2% seroprevalence, using ELISA test. Serum samples from a Q-fever-suspicious herd resulted, however, in 6% (CFT) and 53% (ELISA) positive reactions. A comparison between CFT and ELISA showed both a correlation of the two test methods that increased with higher CFT titration levels and positive reactions using ELISA for 9.4% of the serums that had tested negative using CFT. The results of the present study reveal that ticks and their excrements are important vectors of transmission of Q-fever in Baden-Wuerttemberg. Investigations on C. burnetii using PCR as well as serological surveys of sheep are important instruments for diagnosis and disease control of Q-fever.
Assuntos
Vetores Aracnídeos/microbiologia , Coxiella burnetii/isolamento & purificação , Dermacentor/microbiologia , Febre Q/veterinária , Doenças dos Ovinos/epidemiologia , Animais , Anticorpos Antibacterianos/sangue , Testes de Fixação de Complemento/veterinária , Coxiella burnetii/imunologia , Ensaio de Imunoadsorção Enzimática/veterinária , Feminino , Alemanha/epidemiologia , Humanos , Masculino , Reação em Cadeia da Polimerase/veterinária , Febre Q/epidemiologia , Febre Q/microbiologia , Estudos Soroepidemiológicos , Ovinos , Doenças dos Ovinos/microbiologia , ZoonosesRESUMO
Hantavirus infections are known in Germany since the 1980s. While the overall antibody prevalence against hantaviruses in the general human population was estimated to be about 1-2%, an average of 100-200 clinical cases are recorded annually. In the years 2005 and 2007 in particular, a large increase of the number of human hantavirus infections in Germany was observed. The most affected regions were located in the federal states of Baden-Wuerttemberg, Bavaria, North Rhine Westphalia, and Lower Saxony. In contrast to the well-documented situation in humans, the knowledge of the geographical distribution and frequency of hantavirus infections in their rodent reservoirs as well as any changes thereof was very limited. Hence, the network "Rodent-borne pathogens" was established in Germany allowing synergistic investigations of the rodent population dynamics, the prevalence and evolution of hantaviruses and other rodent-associated pathogens as well as their underlying mechanisms in order to understand their impact on the frequency of human infections. A monitoring of hantaviruses in rodents from endemic regions (Baden-Wuerttemberg, Bavaria, North Rhine Westphalia, Lower Saxony) and regions with a low number of human cases (Mecklenburg Western-Pomerania, Brandenburg, Saxony, Saxony-Anhalt) was initiated. Within outbreak regions, a high prevalence of Puumala virus (PUUV) was detected in bank voles. Initial longitudinal studies in North Rhine Westphalia (city of Cologne), Bavaria (Lower Bavaria), and Lower Saxony (rural region close to Osnabrück) demonstrated a continuing presence of PUUV in the bank vole populations. These longitudinal studies will allow conclusions about the evolution of hantaviruses and other rodent-borne pathogens and changes in their distribution, which can be used for a risk assessment of human infections. This may become very important in order to evaluate changes in the epidemiology of rodent-borne pathogens in the light of expected global climate changes in the future.
Assuntos
Infecções por Hantavirus/veterinária , Virus Puumala/isolamento & purificação , Roedores/virologia , Animais , Geografia , Alemanha/epidemiologia , Infecções por Hantavirus/epidemiologia , Humanos , Incidência , Estudos Longitudinais , Virus Puumala/classificação , Virus Puumala/genética , Estudos SoroepidemiológicosRESUMO
A knowledge of the distribution of tick-borne agents and the related risks of contracting diseases are essential to ensure an appropriate response between hysteria and disregard, and to decide the appropriateness of vaccination. Illustrated by the German town of Lohr a. M. (Bavaria), the prevalence of TBE-virus and Borrelia burgdorferi in ticks was investigated and compared, and the respective risk of contracting disease from a bite assessed. Using the polymerase chain reaction (PCR), a total of 1657 ticks obtained from five different biotopes around Lohr were examined for the TBE-virus, and 408 ticks for Borrelia burgdorferi. The results were compared with earlier findings in other regions of Germany. The risk of contracting illness was estimated on the basis of transmission and manifestation rates, together with epidemiological data from the region. The prevalence of TBE-virus was 0.12% (95% CI: 0.05-0.44%) in the ticks investigated. This is comparable with that in 4 other regions rated as TBE-risk regions, but significantly lower than that in rated high-risk regions. Borrelia burgdorferi was detected in 14.9% (11.8-19.0%) of adult ticks, roughly twice the prevalence found in nymphs (7.2%, range 4.6-11.7%). On the basis of these prevalences, the risk of contracting meningitis/encephalitis from a tick bite is about 1:10,000, and the risk for Lyme Borreliosis is about 1:100, the latter requiring that the tick remains attached for at least 2-3 days.
Assuntos
Borrelia burgdorferi , Vírus da Encefalite Transmitidos por Carrapatos/isolamento & purificação , Encefalite Transmitida por Carrapatos/epidemiologia , Doença de Lyme/epidemiologia , Carrapatos/microbiologia , Carrapatos/virologia , Adulto , Animais , Borrelia burgdorferi/isolamento & purificação , Distribuição de Qui-Quadrado , Intervalos de Confiança , Alemanha/epidemiologia , Humanos , Incidência , Reação em Cadeia da Polimerase , Prevalência , Fatores de Risco , Fatores de TempoRESUMO
The molecular defect leading to Hb Köln has been analyzed by synthetic oligonucleotides. Thus, DNA of 19 nucleotides, in length corresponding to the normal and mutant beta-globin gene sequences, were used to develop a direct assay for the beta k-gene that codes for this most common form of the unstable hemoglobins. The use of synthetic oligonucleotides established that the Hb Köln mutation is due to a G-to-A transition. The conditions described here should result in the determination of all Hb Köln genotypes with a high level of confidence.
Assuntos
Anemia Hemolítica/genética , Hemoglobinopatias/genética , Hemoglobinas Anormais/genética , Anemia Hemolítica/diagnóstico , Sequência de Bases , Hemoglobinopatias/diagnóstico , Humanos , Mutação , Oligodesoxirribonucleotídeos/síntese química , Diagnóstico Pré-NatalRESUMO
DNA haplotype constellations of the beta-globin gene cluster have been analyzed in German families with hemoglobinopathies (Hb Freiburg, Hb Köln, Hb Presbyterian) and beta-thalassemias. The polymorphic patterns obtained were compared to those found in families from Greece, Italy, and Turkey affected by beta-thalassemia syndromes. With the combined analysis of seven restriction site polymorphisms a DNA-diagnostic prediction for additional offspring could be made with an overall frequency of 75% in the four ethnic groups.
Assuntos
DNA/genética , Globinas/genética , Hemoglobinopatias/genética , Polimorfismo Genético , Talassemia/genética , Enzimas de Restrição do DNA , Etnicidade , Feminino , Alemanha Ocidental , Hemoglobinas Anormais/genética , Humanos , Masculino , LinhagemRESUMO
The admixture of maternal tissue is a possible hazard of prenatal diagnosis from chorionic villi. Therefore the tolerable degree of maternal DNA contamination in prenatal diagnosis of sickle cell anemia was investigated by mixing various amounts of DNA from HbS carriers with DNA from normal individuals and sickle cell anemia patients. The results indicate that lower rate of admixed maternal DNA does not prevent an exact direct DNA-diagnosis of sickle cell anemia.
Assuntos
Anemia Falciforme/diagnóstico , Vilosidades Coriônicas , DNA/análise , Diagnóstico Pré-Natal/métodos , Enzimas de Restrição do DNA , Erros de Diagnóstico , Feminino , Globinas/genética , Humanos , GravidezRESUMO
Thalassemia major is a rare disorder in the German population. We describe here the characterization of the beta-globin genes of a German patient homozygous for beta-thalassemia. Gene cloning and sequencing revealed a G to T transversion at the intron 1 donor site of the beta-globin gene on both chromosomes.
Assuntos
Genes , Globinas/genética , Talassemia/genética , Adulto , Análise Mutacional de DNA , Feminino , Alemanha Ocidental , Homozigoto , Humanos , Íntrons , Masculino , LinhagemRESUMO
The molecular defect leading to Haemoglobin (Hb) Freiburg has been analysed using synthetic oligonucleotides. Oligonucleotide probes 19 residues and 16 residues long, corresponding to the normal and mutant beta-globin gene sequences, respectively, were used to develop a direct assay for the beta F-globin gene, which codes for an unstable haemoglobin. Under the conditions described here the use of the respective synthetic oligonucleotides should aid in the determination of all Hb Freiburg genotypes in families at risk with a high level of confidence.
Assuntos
DNA/genética , Hemoglobinas Anormais/genética , Oligonucleotídeos , Feminino , Genótipo , Globinas/genética , Humanos , Masculino , Hibridização de Ácido Nucleico , Linhagem , Homologia de Sequência do Ácido NucleicoRESUMO
Chromosomal DNA from three individuals with familial hemoglobin M (Hb M) Milwaukee was studied by restriction endonuclease analysis. The segregation of the mutant beta-globin gene could be followed through three generations by direct Sst I analysis at the gene level. Various restriction endonucleases were used to confirm the positions of Sst I sites in the delta-beta A- and delta-beta Mi-globin gene regions.
Assuntos
Genes , Globinas/genética , Hemoglobina M/genética , Hemoglobinopatias/genética , Hemoglobinas Anormais/genética , Mutação , Sequência de Bases , Mapeamento Cromossômico , DNA/análise , Enzimas de Restrição do DNA , Feminino , Genes Dominantes , Humanos , Masculino , LinhagemRESUMO
Restriction endonuclease mapping of cellular DNA has been used to identify chromosomes that carry the mutant Hb Presbyterian beta-globin genes in a family with individuals heterozygous for this disease. The presence of the polymorphic Hind III restriction site in the G gamma-globin gene and its absence in the A gamma-globin gene were shown to be in phase with the Hb Presbyterian mutation yielding a haplotype constellation that is diagnostic for any further affected offspring.
Assuntos
DNA/genética , Genes Reguladores , Globinas/genética , Hemoglobinas Anormais/genética , Mutação , Mapeamento Cromossômico , Enzimas de Restrição do DNA , Feminino , Hemoglobinopatias/genética , Humanos , Masculino , LinhagemRESUMO
Nuclear DNA has been analyzed by means of restriction endonuclease mapping procedure to identify chromosomes that carry mutant Hb Köln beta-globin genes in a family with individuals heterozygous for this disease. Inherited DNA polymorphisms within the beta-globin gene cluster yielded a direct linkage of the Hb Köln mutation to haplotype constellations that are diagnostic for further offspring.